##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbvCI BglI BlnI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI FauI FseI
FspI HaeII Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflII 4053 CTTAAG C^TTAAG
AflIII 2210 ACACGT A^C[GA][CT]GT
Alw44I 2578 GTGCAC G^TGCAC
AlwNI 4111 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1790 GGGCCC GGGCC^C
ApaLI 2578 GTGCAC G^TGCAC
AvaI 4133 CCCGAG C^[CT]CG[GA]G
BamHI 224 GGATCC G^GATCC
BcgI 2798 CGAGAATCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 272 TGATCA T^GATCA
BcnI 284 CCCGG CC^[GC]GG
BfrI 4053 CTTAAG C^TTAAG
BglII 3202 AGATCT A^GATCT
BsoBI 4133 CCCGAG C^[CT]CG[GA]G
Bsp1407I 618 TGTACA T^GTACA
BspMI 2706 GCAGGT ACCTGC(4/8)
BsrGI 618 TGTACA T^GTACA
CfoI 765 GCGC GCG^C
EcoNI 116 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 584 GATATC GAT^ATC
EcoT14I 1727 CCTTGG C^C[AT][AT]GG
EcoT22I 646 ATGCAT ATGCA^T
FbaI 272 TGATCA T^GATCA
HapII 285 CCGG C^CGG
HhaI 765 GCGC GCG^C
HinP1I 765 GCGC G^CGC
HpaII 285 CCGG C^CGG
MspI 285 CCGG C^CGG
NciI 284 CCCGG CC^[GC]GG
NheI 1284 GCTAGC G^CTAGC
NsiI 646 ATGCAT ATGCA^T
PshAI 1944 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2569 CTGCAG CTGCA^G
SspBI 618 TGTACA T^GTACA
StuI 14 AGGCCT AGG^CCT
StyI 1727 CCTTGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AocI 157 CCTAAGG CC^T[ACGT]AGG
3281 CCTAAGG
Asp700I 1177 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3088 GAAATTGTTC
BalI 2019 TGGCCA TGG^CCA
2129 TGGCCA
BbrPI 2211 CACGTG CAC^GTG
2575 CACGTG
BciVI 1302 GTATCC GTATCC(6/5)
2318 GTATCC
BlpI 2918 GCTCAGC GC^T[ACGT]AGC
3814 GCTGAGC
Bpu1102I 2918 GCTCAGC GC^T[ACGT]AGC
3814 GCTGAGC
BsaAI 2211 CACGTG [CT]AC^GT[GA]
2575 CACGTG
BsaI 3318 GGTCTC GGTCTC(1/5)
4092 GGTCTC
BspHI 1290 TCATGA T^CATGA
1622 TCATGA
BstAPI 2571 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2933 GCACCGACTGC
BstBI 299 TTCGAA TT^CGAA
1184 TTCGAA
BstXI 135 CCACAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1496 CCATCTCCGTGG
Bsu36I 157 CCTAAGG CC^T[ACGT]AGG
3281 CCTAAGG
CelII 2918 GCTCAGC GC^T[ACGT]AGC
3814 GCTGAGC
DraIII 2978 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3450 CACATAGTG
DsaI 1502 CCGTGG C^C[GA][CT]GG
1849 CCACGG
Eco81I 157 CCTAAGG CC^T[ACGT]AGG
3281 CCTAAGG
HgaI 2167 GACGC GACGC(5/10)
723 GCGTC
MluNI 2019 TGGCCA TGG^CCA
2129 TGGCCA
MscI 2019 TGGCCA TGG^CCA
2129 TGGCCA
NspI 648 GCATGT [GA]CATG^[CT]
752 ACATGC
NspV 299 TTCGAA TT^CGAA
1184 TTCGAA
PmaCI 2211 CACGTG CAC^GTG
2575 CACGTG
PmlI 2211 CACGTG CAC^GTG
2575 CACGTG
RcaI 1290 TCATGA T^CATGA
1622 TCATGA
SacI 3675 GAGCTC GAGCT^C
3817 GAGCTC
SapI 1871 GCTCTTC GCTCTTC(1/4)
2942 GCTCTTC
ScaI 2864 AGTACT AGT^ACT
3991 AGTACT
SfuI 299 TTCGAA TT^CGAA
1184 TTCGAA
SpeI 3994 ACTAGT A^CTAGT
4190 ACTAGT
SspI 1938 AATATT AAT^ATT
4337 AATATT
XmnI 1177 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3088 GAAATTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DrdI EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII