################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BanI BbeI BbvCI BglI BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI FauI FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflII 4053 CTTAAG C^TTAAG AflIII 2210 ACACGT A^C[GA][CT]GT Alw44I 2578 GTGCAC G^TGCAC AlwNI 4111 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1790 GGGCCC GGGCC^C ApaLI 2578 GTGCAC G^TGCAC AvaI 4133 CCCGAG C^[CT]CG[GA]G BamHI 224 GGATCC G^GATCC BcgI 2798 CGAGAATCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 272 TGATCA T^GATCA BcnI 284 CCCGG CC^[GC]GG BfrI 4053 CTTAAG C^TTAAG BglII 3202 AGATCT A^GATCT BsoBI 4133 CCCGAG C^[CT]CG[GA]G Bsp1407I 618 TGTACA T^GTACA BspMI 2706 GCAGGT ACCTGC(4/8) BsrGI 618 TGTACA T^GTACA CfoI 765 GCGC GCG^C EcoNI 116 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 584 GATATC GAT^ATC EcoT14I 1727 CCTTGG C^C[AT][AT]GG EcoT22I 646 ATGCAT ATGCA^T FbaI 272 TGATCA T^GATCA HapII 285 CCGG C^CGG HhaI 765 GCGC GCG^C HinP1I 765 GCGC G^CGC HpaII 285 CCGG C^CGG MspI 285 CCGG C^CGG NciI 284 CCCGG CC^[GC]GG NheI 1284 GCTAGC G^CTAGC NsiI 646 ATGCAT ATGCA^T PshAI 1944 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2569 CTGCAG CTGCA^G SspBI 618 TGTACA T^GTACA StuI 14 AGGCCT AGG^CCT StyI 1727 CCTTGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AocI 157 CCTAAGG CC^T[ACGT]AGG 3281 CCTAAGG Asp700I 1177 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3088 GAAATTGTTC BalI 2019 TGGCCA TGG^CCA 2129 TGGCCA BbrPI 2211 CACGTG CAC^GTG 2575 CACGTG BciVI 1302 GTATCC GTATCC(6/5) 2318 GTATCC BlpI 2918 GCTCAGC GC^T[ACGT]AGC 3814 GCTGAGC Bpu1102I 2918 GCTCAGC GC^T[ACGT]AGC 3814 GCTGAGC BsaAI 2211 CACGTG [CT]AC^GT[GA] 2575 CACGTG BsaI 3318 GGTCTC GGTCTC(1/5) 4092 GGTCTC BspHI 1290 TCATGA T^CATGA 1622 TCATGA BstAPI 2571 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2933 GCACCGACTGC BstBI 299 TTCGAA TT^CGAA 1184 TTCGAA BstXI 135 CCACAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1496 CCATCTCCGTGG Bsu36I 157 CCTAAGG CC^T[ACGT]AGG 3281 CCTAAGG CelII 2918 GCTCAGC GC^T[ACGT]AGC 3814 GCTGAGC DraIII 2978 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 3450 CACATAGTG DsaI 1502 CCGTGG C^C[GA][CT]GG 1849 CCACGG Eco81I 157 CCTAAGG CC^T[ACGT]AGG 3281 CCTAAGG HgaI 2167 GACGC GACGC(5/10) 723 GCGTC MluNI 2019 TGGCCA TGG^CCA 2129 TGGCCA MscI 2019 TGGCCA TGG^CCA 2129 TGGCCA NspI 648 GCATGT [GA]CATG^[CT] 752 ACATGC NspV 299 TTCGAA TT^CGAA 1184 TTCGAA PmaCI 2211 CACGTG CAC^GTG 2575 CACGTG PmlI 2211 CACGTG CAC^GTG 2575 CACGTG RcaI 1290 TCATGA T^CATGA 1622 TCATGA SacI 3675 GAGCTC GAGCT^C 3817 GAGCTC SapI 1871 GCTCTTC GCTCTTC(1/4) 2942 GCTCTTC ScaI 2864 AGTACT AGT^ACT 3991 AGTACT SfuI 299 TTCGAA TT^CGAA 1184 TTCGAA SpeI 3994 ACTAGT A^CTAGT 4190 ACTAGT SspI 1938 AATATT AAT^ATT 4337 AATATT XmnI 1177 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3088 GAAATTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DrdI EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII