##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
Alw44I ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI BbeI BbvCI
BciVI BclI BfrI BglII BsiWI
BsmI BspDI BssHII Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoRV
FbaI FseI HindIII KasI KpnI
KspI MluI NarI NotI NruI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SspI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 3371 GTCTAC GT^[AC][GT]AC
AccIII 580 TCCGGA T^CCGGA
AcyI 1274 GACGCC G[GA]^CG[CT]C
AgeI 1323 ACCGGT A^CCGGT
Aor51HI 3128 AGCGCT AGC^GCT
AvrII 4243 CCTAGG C^CTAGG
BamHI 2171 GGATCC G^GATCC
BbrPI 85 CACGTG CAC^GTG
BlnI 4243 CCTAGG C^CTAGG
BsaAI 85 CACGTG [CT]AC^GT[GA]
BsaBI 4989 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1274 GACGCC G[GA]^CG[CT]C
BseAI 580 TCCGGA T^CCGGA
BsmBI 3625 CGTCTC CGTCTC(1/5)
BspEI 580 TCCGGA T^CCGGA
BspHI 3854 TCATGA T^CATGA
BspLU11I 2564 ACATGT A^CATGT
BsrDI 3447 GCAATG GCAATG(2/0)
BssSI 1268 CTCGTG CACGAG(-5/-1)
DraIII 1016 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3128 AGCGCT AGC^GCT
EcoRI 4566 GAATTC G^AATTC
EcoT22I 455 ATGCAT ATGCA^T
Hin1I 1274 GACGCC G[GA]^CG[CT]C
HpaI 3832 GTTAAC GTT^AAC
MamI 4989 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 336 CAATTG C^AATTG
MroI 580 TCCGGA T^CCGGA
MunI 336 CAATTG C^AATTG
NdeI 67 CATATG CA^TATG
NheI 4456 GCTAGC G^CTAGC
NsiI 455 ATGCAT ATGCA^T
PinAI 1323 ACCGGT A^CCGGT
PmaCI 85 CACGTG CAC^GTG
PmlI 85 CACGTG CAC^GTG
RcaI 3854 TCATGA T^CATGA
SbfI 1443 CCTGCAGG CCTGCA^GG
SfiI 4256 GGCCACTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 453 GCATGC GCATG^C
Sse8387I 1443 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 4566 GAATTC [GA]^AATT[CT]
4734 GAATTT
AflIII 86 ACGTGT A^C[GA][CT]GT
2564 ACATGT
ApoI 4566 GAATTC [GA]^AATT[CT]
4734 GAATTT
AspI 622 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4230 GACAGAGTC
AviII 434 TGCGCA TGC^GCA
2363 TGCGCA
BbsI 1763 GAAGAC GAAGAC(2/6)
4740 GAAGAC
BpuAI 1763 GAAGAC GAAGAC(2/6)
4740 GAAGAC
BsiEI 558 CGACCG CG[GA][CT]^CG
2904 CGGTCG
Bsp1407I 4770 TGTACA T^GTACA
4975 TGTACA
BsrGI 4770 TGTACA T^GTACA
4975 TGTACA
DrdI 212 GACCCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1618 GACGTTGGTGTC
FspI 434 TGCGCA TGC^GCA
2363 TGCGCA
HincII 262 GTCAAC GT[CT]^[GA]AC
3832 GTTAAC
HindII 262 GTCAAC GT[CT]^[GA]AC
3832 GTTAAC
MseI 3833 TTAA T^TAA
4157 TTAA
NcoI 3144 CCATGG C^CATGG
3191 CCATGG
PaeR7I 956 CTCGAG C^TCGAG
3072 CTCGAG
PflFI 622 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4230 GACAGAGTC
SapI 3058 GAAGAGC GCTCTTC(1/4)
72 GCTCTTC
SspBI 4770 TGTACA T^GTACA
4975 TGTACA
Tru9I 3833 TTAA T^TAA
4157 TTAA
Tth111I 622 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4230 GACAGAGTC
XhoI 956 CTCGAG C^TCGAG
3072 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI