################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII Acc65I AclI AflII AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI BbeI BbvCI BciVI BclI BfrI BglII BsiWI BsmI BspDI BssHII Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRV FbaI FseI HindIII KasI KpnI KspI MluI NarI NotI NruI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 3371 GTCTAC GT^[AC][GT]AC AccIII 580 TCCGGA T^CCGGA AcyI 1274 GACGCC G[GA]^CG[CT]C AgeI 1323 ACCGGT A^CCGGT Aor51HI 3128 AGCGCT AGC^GCT AvrII 4243 CCTAGG C^CTAGG BamHI 2171 GGATCC G^GATCC BbrPI 85 CACGTG CAC^GTG BlnI 4243 CCTAGG C^CTAGG BsaAI 85 CACGTG [CT]AC^GT[GA] BsaBI 4989 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1274 GACGCC G[GA]^CG[CT]C BseAI 580 TCCGGA T^CCGGA BsmBI 3625 CGTCTC CGTCTC(1/5) BspEI 580 TCCGGA T^CCGGA BspHI 3854 TCATGA T^CATGA BspLU11I 2564 ACATGT A^CATGT BsrDI 3447 GCAATG GCAATG(2/0) BssSI 1268 CTCGTG CACGAG(-5/-1) DraIII 1016 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 3128 AGCGCT AGC^GCT EcoRI 4566 GAATTC G^AATTC EcoT22I 455 ATGCAT ATGCA^T Hin1I 1274 GACGCC G[GA]^CG[CT]C HpaI 3832 GTTAAC GTT^AAC MamI 4989 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 336 CAATTG C^AATTG MroI 580 TCCGGA T^CCGGA MunI 336 CAATTG C^AATTG NdeI 67 CATATG CA^TATG NheI 4456 GCTAGC G^CTAGC NsiI 455 ATGCAT ATGCA^T PinAI 1323 ACCGGT A^CCGGT PmaCI 85 CACGTG CAC^GTG PmlI 85 CACGTG CAC^GTG RcaI 3854 TCATGA T^CATGA SbfI 1443 CCTGCAGG CCTGCA^GG SfiI 4256 GGCCACTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 453 GCATGC GCATG^C Sse8387I 1443 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 4566 GAATTC [GA]^AATT[CT] 4734 GAATTT AflIII 86 ACGTGT A^C[GA][CT]GT 2564 ACATGT ApoI 4566 GAATTC [GA]^AATT[CT] 4734 GAATTT AspI 622 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4230 GACAGAGTC AviII 434 TGCGCA TGC^GCA 2363 TGCGCA BbsI 1763 GAAGAC GAAGAC(2/6) 4740 GAAGAC BpuAI 1763 GAAGAC GAAGAC(2/6) 4740 GAAGAC BsiEI 558 CGACCG CG[GA][CT]^CG 2904 CGGTCG Bsp1407I 4770 TGTACA T^GTACA 4975 TGTACA BsrGI 4770 TGTACA T^GTACA 4975 TGTACA DrdI 212 GACCCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1618 GACGTTGGTGTC FspI 434 TGCGCA TGC^GCA 2363 TGCGCA HincII 262 GTCAAC GT[CT]^[GA]AC 3832 GTTAAC HindII 262 GTCAAC GT[CT]^[GA]AC 3832 GTTAAC MseI 3833 TTAA T^TAA 4157 TTAA NcoI 3144 CCATGG C^CATGG 3191 CCATGG PaeR7I 956 CTCGAG C^TCGAG 3072 CTCGAG PflFI 622 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4230 GACAGAGTC SapI 3058 GAAGAGC GCTCTTC(1/4) 72 GCTCTTC SspBI 4770 TGTACA T^GTACA 4975 TGTACA Tru9I 3833 TTAA T^TAA 4157 TTAA Tth111I 622 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4230 GACAGAGTC XhoI 956 CTCGAG C^TCGAG 3072 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI