##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
AseI AsnI Asp718I AspEI AspI
AviII BalI BamHI BcgI BseAI
BsiWI BsmBI BspDI BspEI BspLU11I
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraIII Eam1105I FseI FspI
HgaI KpnI MluI MluNI MroI
MscI NcoI NdeI NruI NspV
PacI PaeR7I PflFI PmeI PpuMI
PshBI Psp1406I PvuI RsrII SalI
SbfI SfiI SfuI SmaI SnaBI
Sse8387I Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 3442 GTCTAC GT^[AC][GT]AC
AcyI 424 GGCGCC G[GA]^CG[CT]C
AflII 3971 CTTAAG C^TTAAG
AgeI 325 ACCGGT A^CCGGT
Alw44I 2817 GTGCAC G^TGCAC
Aor51HI 72 AGCGCT AGC^GCT
ApaI 54 GGGCCC GGGCC^C
ApaLI 2817 GTGCAC G^TGCAC
AscI 190 GGCGCGCC GG^CGCGCC
AvrII 172 CCTAGG C^CTAGG
BbeI 424 GGCGCC GGCGC^C
BbrPI 2394 CACGTG CAC^GTG
BbvCI 2208 CCTCAGC CCTCAGC(-5/-2)
BclI 4189 TGATCA T^GATCA
BfrI 3971 CTTAAG C^TTAAG
BglII 2705 AGATCT A^GATCT
BlnI 172 CCTAGG C^CTAGG
BsaBI 4549 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 424 GGCGCC G[GA]^CG[CT]C
BsaWI 325 ACCGGT [AT]^CCGG[AT]
BsmI 4212 GCATTC GAATGC(1/-1)
BspMI 4335 ACCTGC ACCTGC(4/8)
BsrBI 226 CCGCTC CCGCTC(-3/-3)
BssHII 191 GCGCGC G^CGCGC
BstAPI 1683 GCAGATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2125 GGTCACC G^GT[ACGT]ACC
BstPI 2125 GGTCACC G^GT[ACGT]ACC
BstXI 568 CCACATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2873 GACATTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 72 AGCGCT AGC^GCT
EcoO65I 2125 GGTCACC G^GT[ACGT]ACC
EcoRI 901 GAATTC G^AATTC
FbaI 4189 TGATCA T^GATCA
Hin1I 424 GGCGCC G[GA]^CG[CT]C
HpaI 4286 GTTAAC GTT^AAC
KasI 424 GGCGCC G^GCGCC
MamI 4549 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3627 CAATTG C^AATTG
MunI 3627 CAATTG C^AATTG
NarI 424 GGCGCC GG^CGCC
NotI 156 GCGGCCGC GC^GGCCGC
PflMI 2152 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 325 ACCGGT A^CCGGT
PmaCI 2394 CACGTG CAC^GTG
PmlI 2394 CACGTG CAC^GTG
PshAI 1881 GACGACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2922 GAGCTC GAGCT^C
SexAI 3454 ACCTGGT A^CC[AT]GGT
SfoI 424 GGCGCC GGC^GCC
SmlI 3971 CTTAAG C^T[CT][GA]AG
SpeI 3014 ACTAGT A^CTAGT
SphI 3815 GCATGC GCATG^C
StuI 169 AGGCCT AGG^CCT
SwaI 4305 ATTTAAAT ATTT^AAAT
Van91I 2152 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 2499 ACACGT A^C[GA][CT]GT
3990 ACGTGT
AlwNI 2622 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
3194 CAGCATCTG
AocI 196 CCTGAGG CC^T[ACGT]AGG
3402 CCTCAGG
AspHI 2817 GTGCAC G[AT]GC[AT]^C
2922 GAGCTC
AvaI 252 CCCGAG C^[CT]CG[GA]G
402 CCCGAG
AvaII 1513 GGACC G^G[AT]CC
2190 GGACC
BciVI 866 GGATAC GTATCC(6/5)
1601 GGATAC
BcnI 23 CCGGG CC^[GC]GG
3472 CCCGG
BglI 162 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
383 GCCGGAGAGGC
BpmI 68 CTCCAG CTGGAG(16/14)
661 CTGGAG
BsiHKAI 2817 GTGCAC G[AT]GC[AT]^C
2922 GAGCTC
BsoBI 252 CCCGAG C^[CT]CG[GA]G
402 CCCGAG
Bsp1407I 2637 TGTACA T^GTACA
2653 TGTACA
BsrGI 2637 TGTACA T^GTACA
2653 TGTACA
Bsu36I 196 CCTGAGG CC^T[ACGT]AGG
3402 CCTCAGG
DsaI 280 CCGCGG C^C[GA][CT]GG
367 CCGCGG
EagI 157 CGGCCG C^GGCCG
185 CGGCCG
EclXI 157 CGGCCG C^GGCCG
185 CGGCCG
Eco52I 157 CGGCCG C^GGCCG
185 CGGCCG
Eco81I 196 CCTGAGG CC^T[ACGT]AGG
3402 CCTCAGG
EcoRV 1203 GATATC GAT^ATC
1323 GATATC
EcoT14I 172 CCTAGG C^C[AT][AT]GG
2086 CCAAGG
EcoT22I 2556 ATGCAT ATGCA^T
4210 ATGCAT
HaeII 72 AGCGCT [GA]GCGC^[CT]
424 GGCGCC
HincII 3492 GTCAAC GT[CT]^[GA]AC
4286 GTTAAC
HindII 3492 GTCAAC GT[CT]^[GA]AC
4286 GTTAAC
KspI 280 CCGCGG CCGC^GG
367 CCGCGG
NaeI 187 GCCGGC GCC^GGC
1666 GCCGGC
NciI 23 CCGGG CC^[GC]GG
3472 CCCGG
NgoMIV 187 GCCGGC G^CCGGC
1666 GCCGGC
NheI 3560 GCTAGC G^CTAGC
4054 GCTAGC
NsiI 2556 ATGCAT ATGCA^T
4210 ATGCAT
NspI 3496 ACATGC [GA]CATG^[CT]
3815 GCATGC
PstI 1645 CTGCAG CTGCA^G
4250 CTGCAG
PvuII 768 CAGCTG CAG^CTG
2641 CAGCTG
SacII 280 CCGCGG CCGC^GG
367 CCGCGG
SapI 887 GAAGAGC GCTCTTC(1/4)
2919 GAAGAGC
SgrAI 85 CACCGGCG C[GA]^CCGG[CT]G
148 CACCGGCG
SspBI 2637 TGTACA T^GTACA
2653 TGTACA
SspI 1834 AATATT AAT^ATT
4039 AATATT
StyI 172 CCTAGG C^C[AT][AT]GG
2086 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I BanI BanII
BbsI BbvI BfaI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiEI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI