################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AccIII AclI AhdI AseI AsnI Asp718I AspEI AspI AviII BalI BamHI BcgI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraIII Eam1105I FseI FspI HgaI KpnI MluI MluNI MroI MscI NcoI NdeI NruI NspV PacI PaeR7I PflFI PmeI PpuMI PshBI Psp1406I PvuI RsrII SalI SbfI SfiI SfuI SmaI SnaBI Sse8387I Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 3442 GTCTAC GT^[AC][GT]AC AcyI 424 GGCGCC G[GA]^CG[CT]C AflII 3971 CTTAAG C^TTAAG AgeI 325 ACCGGT A^CCGGT Alw44I 2817 GTGCAC G^TGCAC Aor51HI 72 AGCGCT AGC^GCT ApaI 54 GGGCCC GGGCC^C ApaLI 2817 GTGCAC G^TGCAC AscI 190 GGCGCGCC GG^CGCGCC AvrII 172 CCTAGG C^CTAGG BbeI 424 GGCGCC GGCGC^C BbrPI 2394 CACGTG CAC^GTG BbvCI 2208 CCTCAGC CCTCAGC(-5/-2) BclI 4189 TGATCA T^GATCA BfrI 3971 CTTAAG C^TTAAG BglII 2705 AGATCT A^GATCT BlnI 172 CCTAGG C^CTAGG BsaBI 4549 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 424 GGCGCC G[GA]^CG[CT]C BsaWI 325 ACCGGT [AT]^CCGG[AT] BsmI 4212 GCATTC GAATGC(1/-1) BspMI 4335 ACCTGC ACCTGC(4/8) BsrBI 226 CCGCTC CCGCTC(-3/-3) BssHII 191 GCGCGC G^CGCGC BstAPI 1683 GCAGATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2125 GGTCACC G^GT[ACGT]ACC BstPI 2125 GGTCACC G^GT[ACGT]ACC BstXI 568 CCACATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 2873 GACATTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 72 AGCGCT AGC^GCT EcoO65I 2125 GGTCACC G^GT[ACGT]ACC EcoRI 901 GAATTC G^AATTC FbaI 4189 TGATCA T^GATCA Hin1I 424 GGCGCC G[GA]^CG[CT]C HpaI 4286 GTTAAC GTT^AAC KasI 424 GGCGCC G^GCGCC MamI 4549 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3627 CAATTG C^AATTG MunI 3627 CAATTG C^AATTG NarI 424 GGCGCC GG^CGCC NotI 156 GCGGCCGC GC^GGCCGC PflMI 2152 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 325 ACCGGT A^CCGGT PmaCI 2394 CACGTG CAC^GTG PmlI 2394 CACGTG CAC^GTG PshAI 1881 GACGACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2922 GAGCTC GAGCT^C SexAI 3454 ACCTGGT A^CC[AT]GGT SfoI 424 GGCGCC GGC^GCC SmlI 3971 CTTAAG C^T[CT][GA]AG SpeI 3014 ACTAGT A^CTAGT SphI 3815 GCATGC GCATG^C StuI 169 AGGCCT AGG^CCT SwaI 4305 ATTTAAAT ATTT^AAAT Van91I 2152 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AflIII 2499 ACACGT A^C[GA][CT]GT 3990 ACGTGT AlwNI 2622 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG 3194 CAGCATCTG AocI 196 CCTGAGG CC^T[ACGT]AGG 3402 CCTCAGG AspHI 2817 GTGCAC G[AT]GC[AT]^C 2922 GAGCTC AvaI 252 CCCGAG C^[CT]CG[GA]G 402 CCCGAG AvaII 1513 GGACC G^G[AT]CC 2190 GGACC BciVI 866 GGATAC GTATCC(6/5) 1601 GGATAC BcnI 23 CCGGG CC^[GC]GG 3472 CCCGG BglI 162 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 383 GCCGGAGAGGC BpmI 68 CTCCAG CTGGAG(16/14) 661 CTGGAG BsiHKAI 2817 GTGCAC G[AT]GC[AT]^C 2922 GAGCTC BsoBI 252 CCCGAG C^[CT]CG[GA]G 402 CCCGAG Bsp1407I 2637 TGTACA T^GTACA 2653 TGTACA BsrGI 2637 TGTACA T^GTACA 2653 TGTACA Bsu36I 196 CCTGAGG CC^T[ACGT]AGG 3402 CCTCAGG DsaI 280 CCGCGG C^C[GA][CT]GG 367 CCGCGG EagI 157 CGGCCG C^GGCCG 185 CGGCCG EclXI 157 CGGCCG C^GGCCG 185 CGGCCG Eco52I 157 CGGCCG C^GGCCG 185 CGGCCG Eco81I 196 CCTGAGG CC^T[ACGT]AGG 3402 CCTCAGG EcoRV 1203 GATATC GAT^ATC 1323 GATATC EcoT14I 172 CCTAGG C^C[AT][AT]GG 2086 CCAAGG EcoT22I 2556 ATGCAT ATGCA^T 4210 ATGCAT HaeII 72 AGCGCT [GA]GCGC^[CT] 424 GGCGCC HincII 3492 GTCAAC GT[CT]^[GA]AC 4286 GTTAAC HindII 3492 GTCAAC GT[CT]^[GA]AC 4286 GTTAAC KspI 280 CCGCGG CCGC^GG 367 CCGCGG NaeI 187 GCCGGC GCC^GGC 1666 GCCGGC NciI 23 CCGGG CC^[GC]GG 3472 CCCGG NgoMIV 187 GCCGGC G^CCGGC 1666 GCCGGC NheI 3560 GCTAGC G^CTAGC 4054 GCTAGC NsiI 2556 ATGCAT ATGCA^T 4210 ATGCAT NspI 3496 ACATGC [GA]CATG^[CT] 3815 GCATGC PstI 1645 CTGCAG CTGCA^G 4250 CTGCAG PvuII 768 CAGCTG CAG^CTG 2641 CAGCTG SacII 280 CCGCGG CCGC^GG 367 CCGCGG SapI 887 GAAGAGC GCTCTTC(1/4) 2919 GAAGAGC SgrAI 85 CACCGGCG C[GA]^CCGG[CT]G 148 CACCGGCG SspBI 2637 TGTACA T^GTACA 2653 TGTACA SspI 1834 AATATT AAT^ATT 4039 AATATT StyI 172 CCTAGG C^C[AT][AT]GG 2086 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI Asp700I BanI BanII BbsI BbvI BfaI BlpI BmyI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI