##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AhdI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       BalI        BamHI       BcgI        BseAI       
BsiWI       BsmBI       BspDI       BspEI       BspLU11I    
BssSI       Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraIII      Eam1105I    FseI        FspI        
HgaI        KpnI        MluI        MluNI       MroI        
MscI        NcoI        NdeI        NruI        NspV        
PacI        PaeR7I      PflFI       PmeI        PpuMI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SbfI        SfiI        SfuI        SmaI        SnaBI       
Sse8387I    Tth111I     XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccI         3442  GTCTAC               GT^[AC][GT]AC
AcyI          424  GGCGCC               G[GA]^CG[CT]C
AflII        3971  CTTAAG               C^TTAAG
AgeI          325  ACCGGT               A^CCGGT
Alw44I       2817  GTGCAC               G^TGCAC
Aor51HI        72  AGCGCT               AGC^GCT
ApaI           54  GGGCCC               GGGCC^C
ApaLI        2817  GTGCAC               G^TGCAC
AscI          190  GGCGCGCC             GG^CGCGCC
AvrII         172  CCTAGG               C^CTAGG
BbeI          424  GGCGCC               GGCGC^C
BbrPI        2394  CACGTG               CAC^GTG
BbvCI        2208  CCTCAGC              CCTCAGC(-5/-2)
BclI         4189  TGATCA               T^GATCA
BfrI         3971  CTTAAG               C^TTAAG
BglII        2705  AGATCT               A^GATCT
BlnI          172  CCTAGG               C^CTAGG
BsaBI        4549  GATTCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         424  GGCGCC               G[GA]^CG[CT]C
BsaWI         325  ACCGGT               [AT]^CCGG[AT]
BsmI         4212  GCATTC               GAATGC(1/-1)
BspMI        4335  ACCTGC               ACCTGC(4/8)
BsrBI         226  CCGCTC               CCGCTC(-3/-3)
BssHII        191  GCGCGC               G^CGCGC
BstAPI       1683  GCAGATATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2125  GGTCACC              G^GT[ACGT]ACC
BstPI        2125  GGTCACC              G^GT[ACGT]ACC
BstXI         568  CCACATTTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI         2873  GACATTGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III       72  AGCGCT               AGC^GCT
EcoO65I      2125  GGTCACC              G^GT[ACGT]ACC
EcoRI         901  GAATTC               G^AATTC
FbaI         4189  TGATCA               T^GATCA
Hin1I         424  GGCGCC               G[GA]^CG[CT]C
HpaI         4286  GTTAAC               GTT^AAC
KasI          424  GGCGCC               G^GCGCC
MamI         4549  GATTCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3627  CAATTG               C^AATTG
MunI         3627  CAATTG               C^AATTG
NarI          424  GGCGCC               GG^CGCC
NotI          156  GCGGCCGC             GC^GGCCGC
PflMI        2152  CCAGGCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         325  ACCGGT               A^CCGGT
PmaCI        2394  CACGTG               CAC^GTG
PmlI         2394  CACGTG               CAC^GTG
PshAI        1881  GACGACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         2922  GAGCTC               GAGCT^C
SexAI        3454  ACCTGGT              A^CC[AT]GGT
SfoI          424  GGCGCC               GGC^GCC
SmlI         3971  CTTAAG               C^T[CT][GA]AG
SpeI         3014  ACTAGT               A^CTAGT
SphI         3815  GCATGC               GCATG^C
StuI          169  AGGCCT               AGG^CCT
SwaI         4305  ATTTAAAT             ATTT^AAAT
Van91I       2152  CCAGGCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   41 enzymes) #
###################################

AflIII       2499  ACACGT               A^C[GA][CT]GT
             3990  ACGTGT              
AlwNI        2622  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3194  CAGCATCTG           
AocI          196  CCTGAGG              CC^T[ACGT]AGG
             3402  CCTCAGG             
AspHI        2817  GTGCAC               G[AT]GC[AT]^C
             2922  GAGCTC              
AvaI          252  CCCGAG               C^[CT]CG[GA]G
              402  CCCGAG              
AvaII        1513  GGACC                G^G[AT]CC
             2190  GGACC               
BciVI         866  GGATAC               GTATCC(6/5)
             1601  GGATAC              
BcnI           23  CCGGG                CC^[GC]GG
             3472  CCCGG               
BglI          162  GCCAGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              383  GCCGGAGAGGC         
BpmI           68  CTCCAG               CTGGAG(16/14)
              661  CTGGAG              
BsiHKAI      2817  GTGCAC               G[AT]GC[AT]^C
             2922  GAGCTC              
BsoBI         252  CCCGAG               C^[CT]CG[GA]G
              402  CCCGAG              
Bsp1407I     2637  TGTACA               T^GTACA
             2653  TGTACA              
BsrGI        2637  TGTACA               T^GTACA
             2653  TGTACA              
Bsu36I        196  CCTGAGG              CC^T[ACGT]AGG
             3402  CCTCAGG             
DsaI          280  CCGCGG               C^C[GA][CT]GG
              367  CCGCGG              
EagI          157  CGGCCG               C^GGCCG
              185  CGGCCG              
EclXI         157  CGGCCG               C^GGCCG
              185  CGGCCG              
Eco52I        157  CGGCCG               C^GGCCG
              185  CGGCCG              
Eco81I        196  CCTGAGG              CC^T[ACGT]AGG
             3402  CCTCAGG             
EcoRV        1203  GATATC               GAT^ATC
             1323  GATATC              
EcoT14I       172  CCTAGG               C^C[AT][AT]GG
             2086  CCAAGG              
EcoT22I      2556  ATGCAT               ATGCA^T
             4210  ATGCAT              
HaeII          72  AGCGCT               [GA]GCGC^[CT]
              424  GGCGCC              
HincII       3492  GTCAAC               GT[CT]^[GA]AC
             4286  GTTAAC              
HindII       3492  GTCAAC               GT[CT]^[GA]AC
             4286  GTTAAC              
KspI          280  CCGCGG               CCGC^GG
              367  CCGCGG              
NaeI          187  GCCGGC               GCC^GGC
             1666  GCCGGC              
NciI           23  CCGGG                CC^[GC]GG
             3472  CCCGG               
NgoMIV        187  GCCGGC               G^CCGGC
             1666  GCCGGC              
NheI         3560  GCTAGC               G^CTAGC
             4054  GCTAGC              
NsiI         2556  ATGCAT               ATGCA^T
             4210  ATGCAT              
NspI         3496  ACATGC               [GA]CATG^[CT]
             3815  GCATGC              
PstI         1645  CTGCAG               CTGCA^G
             4250  CTGCAG              
PvuII         768  CAGCTG               CAG^CTG
             2641  CAGCTG              
SacII         280  CCGCGG               CCGC^GG
              367  CCGCGG              
SapI          887  GAAGAGC              GCTCTTC(1/4)
             2919  GAAGAGC             
SgrAI          85  CACCGGCG             C[GA]^CCGG[CT]G
              148  CACCGGCG            
SspBI        2637  TGTACA               T^GTACA
             2653  TGTACA              
SspI         1834  AATATT               AAT^ATT
             4039  AATATT              
StyI          172  CCTAGG               C^C[AT][AT]GG
             2086  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     BanI        BanII       
BbsI        BbvI        BfaI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       EaeI        EarI        EcoNI       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RcaI        RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XbaI        XhoII       
XmnI