##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AclI AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BciVI BglI
BlnI BlpI Bpu1102I BsaBI BsiWI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI HaeII KasI KpnI MamI
MluI MluNI MscI NarI NdeI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2924 GACGTC GACGT^C
AccI 1319 GTAGAC GT^[AC][GT]AC
AccIII 859 TCCGGA T^CCGGA
AflII 4135 CTTAAG C^TTAAG
AflIII 606 ACGTGT A^C[GA][CT]GT
AhdI 701 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2049 GGGCCC GGGCC^C
AspEI 701 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2086 TGCGCA TGC^GCA
BcgI 1550 CGAGTTAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1510 TGATCA T^GATCA
BfrI 4135 CTTAAG C^TTAAG
BsaAI 2322 CACGTA [CT]AC^GT[GA]
BsaWI 859 TCCGGA [AT]^CCGG[AT]
BseAI 859 TCCGGA T^CCGGA
BsiEI 278 CGACCG CG[GA][CT]^CG
BspEI 859 TCCGGA T^CCGGA
BsrBI 662 GAGCGG CCGCTC(-3/-3)
BssHII 1604 GCGCGC G^CGCGC
DraI 3614 TTTAAA TTT^AAA
DrdI 425 GACCATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 701 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2774 GAATTC G^AATTC
EcoT22I 3967 ATGCAT ATGCA^T
FbaI 1510 TGATCA T^GATCA
FspI 2086 TGCGCA TGC^GCA
KspI 7 CCGCGG CCGC^GG
MfeI 3703 CAATTG C^AATTG
MroI 859 TCCGGA T^CCGGA
MunI 3703 CAATTG C^AATTG
NheI 2812 GCTAGC G^CTAGC
NsiI 3967 ATGCAT ATGCA^T
NspI 2259 GCATGC [GA]CATG^[CT]
PaeR7I 1164 CTCGAG C^TCGAG
PflMI 2858 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 7 CCGCGG CCGC^GG
SapI 1375 GCTCTTC GCTCTTC(1/4)
SpeI 3988 ACTAGT A^CTAGT
SphI 2259 GCATGC GCATG^C
SspI 3434 AATATT AAT^ATT
StuI 2983 AGGCCT AGG^CCT
TfiI 3666 GAATC G^A[AT]TC
Van91I 2858 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1164 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AocI 482 CCTCAGG CC^T[ACGT]AGG
974 CCTCAGG
AvaI 21 CCCGAG C^[CT]CG[GA]G
1164 CTCGAG
BsmBI 3235 CGTCTC CGTCTC(1/5)
965 GAGACG
BsmI 1056 GAATGC GAATGC(1/-1)
4464 GCATTC
BsoBI 21 CCCGAG C^[CT]CG[GA]G
1164 CTCGAG
BspHI 1474 TCATGA T^CATGA
2452 TCATGA
Bsu36I 482 CCTCAGG CC^T[ACGT]AGG
974 CCTCAGG
EaeI 282 CGGCCA [CT]^GGCC[GA]
778 TGGCCG
Eco81I 482 CCTCAGG CC^T[ACGT]AGG
974 CCTCAGG
HindIII 1941 AAGCTT A^AGCTT
2509 AAGCTT
MspA1I 7 CCGCGG C[AC]G^C[GT]G
133 CCGCTG
NaeI 182 GCCGGC GCC^GGC
252 GCCGGC
NgoMIV 182 GCCGGC G^CCGGC
252 GCCGGC
PpuMI 56 GGGTCCT [GA]G^G[AT]CC[CT]
2218 AGGTCCT
RcaI 1474 TCATGA T^CATGA
2452 TCATGA
XcmI 1599 CCATAGCGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2385 CCAAGCAAGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII XmnI