##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AccIII AflII AgeI AhdI Alw44I
ApaLI AseI AsnI AspEI BciVI
BfrI BlpI Bpu1102I BsaBI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI Bst1107I
BstZ17I CelII ClaI CpoI DraI
DrdI Eam1105I EcoRI EcoRV FseI
HindIII HpaI MamI MluI MroI
NdeI NotI NruI PacI PinAI
PmeI PshBI PvuI RcaI RsrII
SalI SapI SbfI SexAI SnaBI
SpeI Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2211 GACGTC GACGT^C
Acc65I 4501 GGTACC G^GTACC
AccI 2214 GTCTAC GT^[AC][GT]AC
AclI 491 AACGTT AA^CGTT
AflIII 3421 ACACGT A^C[GA][CT]GT
AscI 102 GGCGCGCC GG^CGCGCC
Asp700I 4536 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4501 GGTACC G^GTACC
BbrPI 3422 CACGTG CAC^GTG
BcgI 1730 GCAGGTCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3666 TGATCA T^GATCA
BsaAI 3422 CACGTG [CT]AC^GT[GA]
BssHII 103 GCGCGC G^CGCGC
BstAPI 1311 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4355 TTCGAA TT^CGAA
BstEII 3255 GGTCACC G^GT[ACGT]ACC
BstPI 3255 GGTCACC G^GT[ACGT]ACC
DraIII 1960 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 52 CGGCCG C^GGCCG
EclXI 52 CGGCCG C^GGCCG
Eco52I 52 CGGCCG C^GGCCG
EcoO65I 3255 GGTCACC G^GT[ACGT]ACC
EcoT22I 2335 ATGCAT ATGCA^T
FbaI 3666 TGATCA T^GATCA
KpnI 4501 GGTACC GGTAC^C
KspI 135 CCGCGG CCGC^GG
MfeI 1744 CAATTG C^AATTG
MunI 1744 CAATTG C^AATTG
NsiI 2335 ATGCAT ATGCA^T
NspV 4355 TTCGAA TT^CGAA
PaeR7I 3143 CTCGAG C^TCGAG
PmaCI 3422 CACGTG CAC^GTG
PmlI 3422 CACGTG CAC^GTG
PshAI 3888 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 491 AACGTT AA^CGTT
SacI 1408 GAGCTC GAGCT^C
SacII 135 CCGCGG CCGC^GG
SfuI 4355 TTCGAA TT^CGAA
SgrAI 2038 CGCCGGCG C[GA]^CCGG[CT]G
SspI 4347 AATATT AAT^ATT
XbaI 2808 TCTAGA T^CTAGA
XcmI 1016 CCACCGAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3143 CTCGAG C^TCGAG
XmnI 4536 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Aor51HI 1416 AGCGCT AGC^GCT
3800 AGCGCT
ApaI 2767 GGGCCC GGGCC^C
3794 GGGCCC
AspHI 1408 GAGCTC G[AT]GC[AT]^C
2305 GAGCAC
AspI 621 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2179 GACACTGTC
AviII 485 TGCGCA TGC^GCA
1937 TGCGCA
AvrII 820 CCTAGG C^CTAGG
4284 CCTAGG
BamHI 17 GGATCC G^GATCC
1037 GGATCC
BglII 2234 AGATCT A^GATCT
4470 AGATCT
BlnI 820 CCTAGG C^CTAGG
4284 CCTAGG
BsaWI 1509 TCCGGT [AT]^CCGG[AT]
3100 TCCGGT
BsiEI 52 CGGCCG CG[GA][CT]^CG
132 CGACCG
BsiHKAI 1408 GAGCTC G[AT]GC[AT]^C
2305 GAGCAC
BsrDI 482 CATTGC GCAATG(2/0)
1458 GCAATG
BsrFI 1955 GCCGGC [GA]^CCGG[CT]
2039 GCCGGC
BssSI 646 CTCGTG CACGAG(-5/-1)
986 CTCGTG
Cfr10I 1955 GCCGGC [GA]^CCGG[CT]
2039 GCCGGC
Eco47III 1416 AGCGCT AGC^GCT
3800 AGCGCT
FspI 485 TGCGCA TGC^GCA
1937 TGCGCA
NaeI 1955 GCCGGC GCC^GGC
2039 GCCGGC
NgoMIV 1955 GCCGGC G^CCGGC
2039 GCCGGC
NheI 867 GCTAGC G^CTAGC
922 GCTAGC
PflFI 621 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2179 GACACTGTC
ScaI 2435 AGTACT AGT^ACT
4538 AGTACT
SfiI 358 GGCCCCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2961 GGCCTACCAGGCC
SphI 434 GCATGC GCATG^C
2333 GCATGC
Tth111I 621 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2179 GACACTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI