################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AccIII AflII AgeI AhdI Alw44I ApaLI AseI AsnI AspEI BciVI BfrI BlpI Bpu1102I BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I EcoRI EcoRV FseI HindIII HpaI MamI MluI MroI NdeI NotI NruI PacI PinAI PmeI PshBI PvuI RcaI RsrII SalI SapI SbfI SexAI SnaBI SpeI Sse8387I SspBI SwaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 2211 GACGTC GACGT^C Acc65I 4501 GGTACC G^GTACC AccI 2214 GTCTAC GT^[AC][GT]AC AclI 491 AACGTT AA^CGTT AflIII 3421 ACACGT A^C[GA][CT]GT AscI 102 GGCGCGCC GG^CGCGCC Asp700I 4536 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4501 GGTACC G^GTACC BbrPI 3422 CACGTG CAC^GTG BcgI 1730 GCAGGTCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 3666 TGATCA T^GATCA BsaAI 3422 CACGTG [CT]AC^GT[GA] BssHII 103 GCGCGC G^CGCGC BstAPI 1311 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 4355 TTCGAA TT^CGAA BstEII 3255 GGTCACC G^GT[ACGT]ACC BstPI 3255 GGTCACC G^GT[ACGT]ACC DraIII 1960 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 52 CGGCCG C^GGCCG EclXI 52 CGGCCG C^GGCCG Eco52I 52 CGGCCG C^GGCCG EcoO65I 3255 GGTCACC G^GT[ACGT]ACC EcoT22I 2335 ATGCAT ATGCA^T FbaI 3666 TGATCA T^GATCA KpnI 4501 GGTACC GGTAC^C KspI 135 CCGCGG CCGC^GG MfeI 1744 CAATTG C^AATTG MunI 1744 CAATTG C^AATTG NsiI 2335 ATGCAT ATGCA^T NspV 4355 TTCGAA TT^CGAA PaeR7I 3143 CTCGAG C^TCGAG PmaCI 3422 CACGTG CAC^GTG PmlI 3422 CACGTG CAC^GTG PshAI 3888 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 491 AACGTT AA^CGTT SacI 1408 GAGCTC GAGCT^C SacII 135 CCGCGG CCGC^GG SfuI 4355 TTCGAA TT^CGAA SgrAI 2038 CGCCGGCG C[GA]^CCGG[CT]G SspI 4347 AATATT AAT^ATT XbaI 2808 TCTAGA T^CTAGA XcmI 1016 CCACCGAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3143 CTCGAG C^TCGAG XmnI 4536 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Aor51HI 1416 AGCGCT AGC^GCT 3800 AGCGCT ApaI 2767 GGGCCC GGGCC^C 3794 GGGCCC AspHI 1408 GAGCTC G[AT]GC[AT]^C 2305 GAGCAC AspI 621 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2179 GACACTGTC AviII 485 TGCGCA TGC^GCA 1937 TGCGCA AvrII 820 CCTAGG C^CTAGG 4284 CCTAGG BamHI 17 GGATCC G^GATCC 1037 GGATCC BglII 2234 AGATCT A^GATCT 4470 AGATCT BlnI 820 CCTAGG C^CTAGG 4284 CCTAGG BsaWI 1509 TCCGGT [AT]^CCGG[AT] 3100 TCCGGT BsiEI 52 CGGCCG CG[GA][CT]^CG 132 CGACCG BsiHKAI 1408 GAGCTC G[AT]GC[AT]^C 2305 GAGCAC BsrDI 482 CATTGC GCAATG(2/0) 1458 GCAATG BsrFI 1955 GCCGGC [GA]^CCGG[CT] 2039 GCCGGC BssSI 646 CTCGTG CACGAG(-5/-1) 986 CTCGTG Cfr10I 1955 GCCGGC [GA]^CCGG[CT] 2039 GCCGGC Eco47III 1416 AGCGCT AGC^GCT 3800 AGCGCT FspI 485 TGCGCA TGC^GCA 1937 TGCGCA NaeI 1955 GCCGGC GCC^GGC 2039 GCCGGC NgoMIV 1955 GCCGGC G^CCGGC 2039 GCCGGC NheI 867 GCTAGC G^CTAGC 922 GCTAGC PflFI 621 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2179 GACACTGTC ScaI 2435 AGTACT AGT^ACT 4538 AGTACT SfiI 358 GGCCCCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2961 GGCCTACCAGGCC SphI 434 GCATGC GCATG^C 2333 GCATGC Tth111I 621 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2179 GACACTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI