##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Aor51HI AscI AseI AsnI Asp700I
Asp718I BcgI BfrI BlpI Bpu1102I
BsaBI BseAI BsiWI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstZ17I
CelII CpoI DrdI Eco47III EcoRV
EcoT22I HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RcaI RsrII SbfI SexAI
SfiI SgrAI SnaBI SphI Sse8387I
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1862 GTCGAC GT^[AC][GT]AC
AclI 4541 AACGTT AA^CGTT
AflIII 3727 ACACGT A^C[GA][CT]GT
AhdI 1833 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3417 CCTGAGG CC^T[ACGT]AGG
AspEI 1833 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2089 GGCGCC GGCGC^C
BbvCI 3429 GCTGAGG CCTCAGC(-5/-2)
BciVI 3972 GTATCC GTATCC(6/5)
BclI 4128 TGATCA T^GATCA
BglII 505 AGATCT A^GATCT
BsmBI 3 CGTCTC CGTCTC(1/5)
BspDI 1272 ATCGAT AT^CGAT
BsrBI 2520 GAGCGG CCGCTC(-3/-3)
BstBI 4264 TTCGAA TT^CGAA
BstEII 2796 GGTCACC G^GT[ACGT]ACC
BstPI 2796 GGTCACC G^GT[ACGT]ACC
Bsu36I 3417 CCTGAGG CC^T[ACGT]AGG
ClaI 1272 ATCGAT AT^CGAT
Eam1105I 1833 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3417 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2796 GGTCACC G^GT[ACGT]ACC
EcoRI 4345 GAATTC G^AATTC
FbaI 4128 TGATCA T^GATCA
FseI 1448 GGCCGGCC GGCCGG^CC
HincII 1862 GTCGAC GT[CT]^[GA]AC
HindII 1862 GTCGAC GT[CT]^[GA]AC
KasI 2089 GGCGCC G^GCGCC
NarI 2089 GGCGCC GG^CGCC
NspV 4264 TTCGAA TT^CGAA
Psp1406I 4541 AACGTT AA^CGTT
SalI 1862 GTCGAC G^TCGAC
ScaI 1132 AGTACT AGT^ACT
SfoI 2089 GGCGCC GGC^GCC
SfuI 4264 TTCGAA TT^CGAA
SpeI 2922 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 4307 AAATTT [GA]^AATT[CT]
4345 GAATTC
Alw44I 137 GTGCAC G^TGCAC
2499 GTGCAC
ApaLI 137 GTGCAC G^TGCAC
2499 GTGCAC
ApoI 4307 AAATTT [GA]^AATT[CT]
4345 GAATTC
AviII 559 TGCGCA TGC^GCA
2740 TGCGCA
AvrII 71 CCTAGG C^CTAGG
2983 CCTAGG
BamHI 145 GGATCC G^GATCC
3361 GGATCC
BbrPI 417 CACGTG CAC^GTG
1014 CACGTG
BlnI 71 CCTAGG C^CTAGG
2983 CCTAGG
BsaAI 417 CACGTG [CT]AC^GT[GA]
1014 CACGTG
BsaWI 1837 TCCGGT [AT]^CCGG[AT]
2363 ACCGGA
Bsp1407I 3264 TGTACA T^GTACA
4290 TGTACA
BsrGI 3264 TGTACA T^GTACA
4290 TGTACA
BstAPI 195 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2873 GCACCCACTGC
DraI 3187 TTTAAA TTT^AAA
3656 TTTAAA
DraIII 1404 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4082 CACCCAGTG
EcoNI 2633 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3365 CCTCCTCTAGG
FspI 559 TGCGCA TGC^GCA
2740 TGCGCA
KspI 33 CCGCGG CCGC^GG
48 CCGCGG
NspI 1306 GCATGT [GA]CATG^[CT]
4512 GCATGT
PmaCI 417 CACGTG CAC^GTG
1014 CACGTG
PmlI 417 CACGTG CAC^GTG
1014 CACGTG
SacII 33 CCGCGG CCGC^GG
48 CCGCGG
SmaI 2346 CCCGGG CCC^GGG
4005 CCCGGG
SmlI 3960 CTTGAG C^T[CT][GA]AG
4499 CTTGAG
SspBI 3264 TGTACA T^GTACA
4290 TGTACA
StuI 1508 AGGCCT AGG^CCT
2413 AGGCCT
XcmI 999 CCAGAGCCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1941 CCAAAAAGAAAATGG
XmaCI 2346 CCCGGG C^CCGGG
4005 CCCGGG
XmaI 2346 CCCGGG C^CCGGG
4005 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII