################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AccII AccIII AciI AclI AcyI AgeI AhdI Aor51HI ApaI AscI AspEI AspI AviII AvrII BbeI BbvCI BcgI BglI BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FauI FseI FspI Hin1I HindIII KasI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NotI NruI NsiI NspV PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 4381 GGTACC G^GTACC AflII 2804 CTTAAG C^TTAAG Alw44I 423 GTGCAC G^TGCAC AocI 1937 CCTGAGG CC^T[ACGT]AGG ApaLI 423 GTGCAC G^TGCAC Asp700I 824 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4381 GGTACC G^GTACC AvaI 1538 CCCGGG C^[CT]CG[GA]G BamHI 182 GGATCC G^GATCC BbrPI 426 CACGTG CAC^GTG BciVI 2346 GTATCC GTATCC(6/5) BfrI 2804 CTTAAG C^TTAAG BseRI 2202 GAGGAG GAGGAG(10/8) BsoBI 1538 CCCGGG C^[CT]CG[GA]G BspMI 1529 GCAGGT ACCTGC(4/8) Bsu36I 1937 CCTGAGG CC^T[ACGT]AGG Eco81I 1937 CCTGAGG CC^T[ACGT]AGG EcoNI 2314 CCTGGAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1818 GATATC GAT^ATC HaeII 550 AGCGCC [GA]GCGC^[CT] HpaI 965 GTTAAC GTT^AAC KpnI 4381 GGTACC GGTAC^C MspA1I 1244 CAGCTG C[AC]G^C[GT]G NheI 1078 GCTAGC G^CTAGC PacI 1827 TTAATTAA TTAAT^TAA PmaCI 426 CACGTG CAC^GTG PmlI 426 CACGTG CAC^GTG PpuMI 1068 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 1244 CAGCTG CAG^CTG ScaI 3494 AGTACT AGT^ACT SexAI 3339 ACCTGGT A^CC[AT]GGT SmaI 1538 CCCGGG CCC^GGG SpeI 3062 ACTAGT A^CTAGT XmaCI 1538 CCCGGG C^CCGGG XmaI 1538 CCCGGG C^CCGGG XmnI 824 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 1090 GTATAC GT^[AC][GT]AC 3689 GTATAC BalI 118 TGGCCA TGG^CCA 4345 TGGCCA BbsI 2974 GAAGAC GAAGAC(2/6) 4302 GTCTTC BglII 2257 AGATCT A^GATCT 3753 AGATCT BpmI 2233 CTCCAG CTGGAG(16/14) 3749 CTCCAG BpuAI 2974 GAAGAC GAAGAC(2/6) 4302 GTCTTC BsaAI 426 CACGTG [CT]AC^GT[GA] 1087 CACGTA BsaI 1958 GAGACC GGTCTC(1/5) 3343 GGTCTC BsmI 1917 GAATGC GAATGC(1/-1) 3876 GCATTC Bst1107I 1090 GTATAC GTA^TAC 3689 GTATAC BstZ17I 1090 GTATAC GTA^TAC 3689 GTATAC CfoI 551 GCGC GCG^C 4724 GCGC DraIII 1899 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG 2952 CACCCGGTG DrdI 3396 GACCTCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3507 GACTCAAATGTC EaeI 118 TGGCCA [CT]^GGCC[GA] 4345 TGGCCA EcoRI 824 GAATTC G^AATTC 4096 GAATTC HhaI 551 GCGC GCG^C 4724 GCGC HinP1I 551 GCGC G^CGC 4724 GCGC MluNI 118 TGGCCA TGG^CCA 4345 TGGCCA MscI 118 TGGCCA TGG^CCA 4345 TGGCCA NcoI 3811 CCATGG C^CATGG 4466 CCATGG NdeI 311 CATATG CA^TATG 2630 CATATG PflMI 372 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1010 CCACAAGTTGG SapI 2531 GAAGAGC GCTCTTC(1/4) 2882 GCTCTTC TaqI 452 TCGA T^CGA 3107 TCGA TthHB8I 452 TCGA T^CGA 3107 TCGA Van91I 372 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1010 CCACAAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BanI BanII BbvI BclI BcnI BfaI BlpI BmyI Bpu1102I BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspHI BspLU11I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HgaI HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI PstI RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI XcmI XhoII