##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccII AccIII AciI AclI
AcyI AgeI AhdI Aor51HI ApaI
AscI AspEI AspI AviII AvrII
BbeI BbvCI BcgI BglI BlnI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FauI FseI FspI Hin1I HindIII
KasI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NotI NruI NsiI NspV
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I StuI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 4381 GGTACC G^GTACC
AflII 2804 CTTAAG C^TTAAG
Alw44I 423 GTGCAC G^TGCAC
AocI 1937 CCTGAGG CC^T[ACGT]AGG
ApaLI 423 GTGCAC G^TGCAC
Asp700I 824 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4381 GGTACC G^GTACC
AvaI 1538 CCCGGG C^[CT]CG[GA]G
BamHI 182 GGATCC G^GATCC
BbrPI 426 CACGTG CAC^GTG
BciVI 2346 GTATCC GTATCC(6/5)
BfrI 2804 CTTAAG C^TTAAG
BseRI 2202 GAGGAG GAGGAG(10/8)
BsoBI 1538 CCCGGG C^[CT]CG[GA]G
BspMI 1529 GCAGGT ACCTGC(4/8)
Bsu36I 1937 CCTGAGG CC^T[ACGT]AGG
Eco81I 1937 CCTGAGG CC^T[ACGT]AGG
EcoNI 2314 CCTGGAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1818 GATATC GAT^ATC
HaeII 550 AGCGCC [GA]GCGC^[CT]
HpaI 965 GTTAAC GTT^AAC
KpnI 4381 GGTACC GGTAC^C
MspA1I 1244 CAGCTG C[AC]G^C[GT]G
NheI 1078 GCTAGC G^CTAGC
PacI 1827 TTAATTAA TTAAT^TAA
PmaCI 426 CACGTG CAC^GTG
PmlI 426 CACGTG CAC^GTG
PpuMI 1068 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1244 CAGCTG CAG^CTG
ScaI 3494 AGTACT AGT^ACT
SexAI 3339 ACCTGGT A^CC[AT]GGT
SmaI 1538 CCCGGG CCC^GGG
SpeI 3062 ACTAGT A^CTAGT
XmaCI 1538 CCCGGG C^CCGGG
XmaI 1538 CCCGGG C^CCGGG
XmnI 824 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1090 GTATAC GT^[AC][GT]AC
3689 GTATAC
BalI 118 TGGCCA TGG^CCA
4345 TGGCCA
BbsI 2974 GAAGAC GAAGAC(2/6)
4302 GTCTTC
BglII 2257 AGATCT A^GATCT
3753 AGATCT
BpmI 2233 CTCCAG CTGGAG(16/14)
3749 CTCCAG
BpuAI 2974 GAAGAC GAAGAC(2/6)
4302 GTCTTC
BsaAI 426 CACGTG [CT]AC^GT[GA]
1087 CACGTA
BsaI 1958 GAGACC GGTCTC(1/5)
3343 GGTCTC
BsmI 1917 GAATGC GAATGC(1/-1)
3876 GCATTC
Bst1107I 1090 GTATAC GTA^TAC
3689 GTATAC
BstZ17I 1090 GTATAC GTA^TAC
3689 GTATAC
CfoI 551 GCGC GCG^C
4724 GCGC
DraIII 1899 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
2952 CACCCGGTG
DrdI 3396 GACCTCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3507 GACTCAAATGTC
EaeI 118 TGGCCA [CT]^GGCC[GA]
4345 TGGCCA
EcoRI 824 GAATTC G^AATTC
4096 GAATTC
HhaI 551 GCGC GCG^C
4724 GCGC
HinP1I 551 GCGC G^CGC
4724 GCGC
MluNI 118 TGGCCA TGG^CCA
4345 TGGCCA
MscI 118 TGGCCA TGG^CCA
4345 TGGCCA
NcoI 3811 CCATGG C^CATGG
4466 CCATGG
NdeI 311 CATATG CA^TATG
2630 CATATG
PflMI 372 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1010 CCACAAGTTGG
SapI 2531 GAAGAGC GCTCTTC(1/4)
2882 GCTCTTC
TaqI 452 TCGA T^CGA
3107 TCGA
TthHB8I 452 TCGA T^CGA
3107 TCGA
Van91I 372 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1010 CCACAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BanI BanII BbvI BclI
BcnI BfaI BlpI BmyI Bpu1102I
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspHI
BspLU11I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HgaI
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI XcmI XhoII