##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AvrII BbvCI BcgI BciVI
BlnI BlpI Bpu1102I BseAI BsiWI
BsmBI BspDI BspEI BsrBI BsrFI
BssHII BstBI BstUI CelII Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoNI EcoT22I FseI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SpeI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AclI 2642 AACGTT AA^CGTT
AcyI 54 GGCGCC G[GA]^CG[CT]C
AflII 1726 CTTAAG C^TTAAG
AflIII 4474 ACATGT A^C[GA][CT]GT
Alw44I 2434 GTGCAC G^TGCAC
ApaI 3635 GGGCCC GGGCC^C
ApaLI 2434 GTGCAC G^TGCAC
AspI 325 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 337 TGCGCA TGC^GCA
BbeI 54 GGCGCC GGCGC^C
BbrPI 9 CACGTG CAC^GTG
BfrI 1726 CTTAAG C^TTAAG
BglI 361 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4072 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 54 GGCGCC G[GA]^CG[CT]C
BsaWI 44 TCCGGT [AT]^CCGG[AT]
BsiEI 163 CGATCG CG[GA][CT]^CG
BsmI 1334 GCATTC GAATGC(1/-1)
Bsp1407I 4039 TGTACA T^GTACA
BspHI 681 TCATGA T^CATGA
BspLU11I 4474 ACATGT A^CATGT
BsrGI 4039 TGTACA T^GTACA
Bst1107I 4482 GTATAC GTA^TAC
BstAPI 907 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4482 GTATAC GTA^TAC
DraIII 1364 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 770 GAATTC G^AATTC
FspI 337 TGCGCA TGC^GCA
HgaI 750 GCGTC GACGC(5/10)
Hin1I 54 GGCGCC G[GA]^CG[CT]C
HpaI 28 GTTAAC GTT^AAC
KasI 54 GGCGCC G^GCGCC
MamI 4072 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 54 GGCGCC GG^CGCC
NcoI 3631 CCATGG C^CATGG
NheI 4463 GCTAGC G^CTAGC
PflFI 325 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 9 CACGTG CAC^GTG
PmlI 9 CACGTG CAC^GTG
PshAI 520 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2642 AACGTT AA^CGTT
PvuI 163 CGATCG CGAT^CG
RcaI 681 TCATGA T^CATGA
SfoI 54 GGCGCC GGC^GCC
SmaI 1136 CCCGGG CCC^GGG
SnaBI 4313 TACGTA TAC^GTA
SphI 3760 GCATGC GCATG^C
SspBI 4039 TGTACA T^GTACA
SspI 4120 AATATT AAT^ATT
Tth111I 325 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3095 TCTAGA T^CTAGA
XcmI 3639 CCAGATCGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1136 CCCGGG C^CCGGG
XmaI 1136 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1466 GTCTAC GT^[AC][GT]AC
4482 GTATAC
AvaI 1136 CCCGGG C^[CT]CG[GA]G
2153 CCCGAG
BalI 3132 TGGCCA TGG^CCA
3926 TGGCCA
BclI 1275 TGATCA T^GATCA
1303 TGATCA
BglII 2865 AGATCT A^GATCT
4077 AGATCT
BsaAI 9 CACGTG [CT]AC^GT[GA]
4313 TACGTA
BsgI 934 GTGCAG GTGCAG(16/14)
1753 GTGCAG
BsoBI 1136 CCCGGG C^[CT]CG[GA]G
2153 CCCGAG
BspMI 237 ACCTGC ACCTGC(4/8)
2902 ACCTGC
BstEII 850 GGTGACC G^GT[ACGT]ACC
4363 GGTGACC
BstPI 850 GGTGACC G^GT[ACGT]ACC
4363 GGTGACC
BstXI 2284 CCAAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4648 CCATGTAACTGG
DrdI 955 GACTCGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2832 GACACTTTTGTC
DsaI 2908 CCACGG C^C[GA][CT]GG
3631 CCATGG
EaeI 3132 TGGCCA [CT]^GGCC[GA]
3926 TGGCCA
EcoO65I 850 GGTGACC G^GT[ACGT]ACC
4363 GGTGACC
EcoRV 3379 GATATC GAT^ATC
3714 GATATC
FbaI 1275 TGATCA T^GATCA
1303 TGATCA
HincII 28 GTTAAC GT[CT]^[GA]AC
2666 GTTGAC
HindII 28 GTTAAC GT[CT]^[GA]AC
2666 GTTGAC
MluNI 3132 TGGCCA TGG^CCA
3926 TGGCCA
MscI 3132 TGGCCA TGG^CCA
3926 TGGCCA
PflMI 1324 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3929 CCAGCCCTTGG
SacI 307 GAGCTC GAGCT^C
4256 GAGCTC
ScaI 942 AGTACT AGT^ACT
2758 AGTACT
Van91I 1324 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3929 CCAGCCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI Aor51HI ApoI Asp700I
AspHI AvaII BamHI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BssSI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI Eco47III Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI