################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AccII AccIII AgeI AhdI AscI AseI AsnI Asp718I AspEI AvrII BbvCI BcgI BciVI BlnI BlpI Bpu1102I BseAI BsiWI BsmBI BspDI BspEI BsrBI BsrFI BssHII BstBI BstUI CelII Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoNI EcoT22I FseI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SpeI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AclI 2642 AACGTT AA^CGTT AcyI 54 GGCGCC G[GA]^CG[CT]C AflII 1726 CTTAAG C^TTAAG AflIII 4474 ACATGT A^C[GA][CT]GT Alw44I 2434 GTGCAC G^TGCAC ApaI 3635 GGGCCC GGGCC^C ApaLI 2434 GTGCAC G^TGCAC AspI 325 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 337 TGCGCA TGC^GCA BbeI 54 GGCGCC GGCGC^C BbrPI 9 CACGTG CAC^GTG BfrI 1726 CTTAAG C^TTAAG BglI 361 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 4072 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 54 GGCGCC G[GA]^CG[CT]C BsaWI 44 TCCGGT [AT]^CCGG[AT] BsiEI 163 CGATCG CG[GA][CT]^CG BsmI 1334 GCATTC GAATGC(1/-1) Bsp1407I 4039 TGTACA T^GTACA BspHI 681 TCATGA T^CATGA BspLU11I 4474 ACATGT A^CATGT BsrGI 4039 TGTACA T^GTACA Bst1107I 4482 GTATAC GTA^TAC BstAPI 907 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 4482 GTATAC GTA^TAC DraIII 1364 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 770 GAATTC G^AATTC FspI 337 TGCGCA TGC^GCA HgaI 750 GCGTC GACGC(5/10) Hin1I 54 GGCGCC G[GA]^CG[CT]C HpaI 28 GTTAAC GTT^AAC KasI 54 GGCGCC G^GCGCC MamI 4072 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 54 GGCGCC GG^CGCC NcoI 3631 CCATGG C^CATGG NheI 4463 GCTAGC G^CTAGC PflFI 325 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 9 CACGTG CAC^GTG PmlI 9 CACGTG CAC^GTG PshAI 520 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2642 AACGTT AA^CGTT PvuI 163 CGATCG CGAT^CG RcaI 681 TCATGA T^CATGA SfoI 54 GGCGCC GGC^GCC SmaI 1136 CCCGGG CCC^GGG SnaBI 4313 TACGTA TAC^GTA SphI 3760 GCATGC GCATG^C SspBI 4039 TGTACA T^GTACA SspI 4120 AATATT AAT^ATT Tth111I 325 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3095 TCTAGA T^CTAGA XcmI 3639 CCAGATCGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1136 CCCGGG C^CCGGG XmaI 1136 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 1466 GTCTAC GT^[AC][GT]AC 4482 GTATAC AvaI 1136 CCCGGG C^[CT]CG[GA]G 2153 CCCGAG BalI 3132 TGGCCA TGG^CCA 3926 TGGCCA BclI 1275 TGATCA T^GATCA 1303 TGATCA BglII 2865 AGATCT A^GATCT 4077 AGATCT BsaAI 9 CACGTG [CT]AC^GT[GA] 4313 TACGTA BsgI 934 GTGCAG GTGCAG(16/14) 1753 GTGCAG BsoBI 1136 CCCGGG C^[CT]CG[GA]G 2153 CCCGAG BspMI 237 ACCTGC ACCTGC(4/8) 2902 ACCTGC BstEII 850 GGTGACC G^GT[ACGT]ACC 4363 GGTGACC BstPI 850 GGTGACC G^GT[ACGT]ACC 4363 GGTGACC BstXI 2284 CCAAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4648 CCATGTAACTGG DrdI 955 GACTCGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2832 GACACTTTTGTC DsaI 2908 CCACGG C^C[GA][CT]GG 3631 CCATGG EaeI 3132 TGGCCA [CT]^GGCC[GA] 3926 TGGCCA EcoO65I 850 GGTGACC G^GT[ACGT]ACC 4363 GGTGACC EcoRV 3379 GATATC GAT^ATC 3714 GATATC FbaI 1275 TGATCA T^GATCA 1303 TGATCA HincII 28 GTTAAC GT[CT]^[GA]AC 2666 GTTGAC HindII 28 GTTAAC GT[CT]^[GA]AC 2666 GTTGAC MluNI 3132 TGGCCA TGG^CCA 3926 TGGCCA MscI 3132 TGGCCA TGG^CCA 3926 TGGCCA PflMI 1324 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3929 CCAGCCCTTGG SacI 307 GAGCTC GAGCT^C 4256 GAGCTC ScaI 942 AGTACT AGT^ACT 2758 AGTACT Van91I 1324 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3929 CCAGCCCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI Aor51HI ApoI Asp700I AspHI AvaII BamHI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BssSI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI Eco47III Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI