##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AspEI AviII AvrII BbeI
BbrPI BcgI BglI BlnI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrFI BssHII BstAPI BstBI BstEII
BstPI Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FseI
FspI KasI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 2693 GACGTC GACGT^C
Acc65I 669 GGTACC G^GTACC
AcyI 2693 GACGTC G[GA]^CG[CT]C
AflIII 4201 ACGTGT A^C[GA][CT]GT
Alw44I 3493 GTGCAC G^TGCAC
AocI 2990 CCTGAGG CC^T[ACGT]AGG
ApaLI 3493 GTGCAC G^TGCAC
Asp718I 669 GGTACC G^GTACC
BalI 1519 TGGCCA TGG^CCA
BbvCI 2441 GCTGAGG CCTCAGC(-5/-2)
BciVI 2005 GGATAC GTATCC(6/5)
BcnI 1559 CCCGG CC^[GC]GG
BlpI 32 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 32 GCTCAGC GC^T[ACGT]AGC
BsaAI 4200 TACGTG [CT]AC^GT[GA]
BsaHI 2693 GACGTC G[GA]^CG[CT]C
BsaI 3431 GGTCTC GGTCTC(1/5)
BsaWI 3372 ACCGGA [AT]^CCGG[AT]
BsgI 2234 GTGCAG GTGCAG(16/14)
BsmBI 903 CGTCTC CGTCTC(1/5)
BsmFI 1447 GGGAC GGGAC(10/14)
Bsp1407I 835 TGTACA T^GTACA
BspHI 409 TCATGA T^CATGA
BsrBI 2001 GAGCGG CCGCTC(-3/-3)
BsrGI 835 TGTACA T^GTACA
BssSI 4574 CTCGTG CACGAG(-5/-1)
Bsu36I 2990 CCTGAGG CC^T[ACGT]AGG
CelII 32 GCTCAGC GC^T[ACGT]AGC
DrdI 3277 GACATTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2990 CCTGAGG CC^T[ACGT]AGG
EcoRI 536 GAATTC G^AATTC
EcoT22I 2621 ATGCAT ATGCA^T
FauI 790 GCGGG CCCGC(4/6)
HgaI 902 GCGTC GACGC(5/10)
Hin1I 2693 GACGTC G[GA]^CG[CT]C
HindIII 3466 AAGCTT A^AGCTT
HpaI 595 GTTAAC GTT^AAC
KpnI 669 GGTACC GGTAC^C
MluNI 1519 TGGCCA TGG^CCA
MscI 1519 TGGCCA TGG^CCA
NciI 1559 CCCGG CC^[GC]GG
NruI 2091 TCGCGA TCG^CGA
NsiI 2621 ATGCAT ATGCA^T
PpuMI 2085 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 3515 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 409 TCATGA T^CATGA
SspBI 835 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1187 GTATAC GT^[AC][GT]AC
3654 GTATAC
AlwNI 1797 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2541 CAGAAACTG
ApaI 14 GGGCCC GGGCC^C
3876 GGGCCC
AseI 986 ATTAAT AT^TAAT
1727 ATTAAT
AsnI 986 ATTAAT AT^TAAT
1727 ATTAAT
AspI 185 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3299 GACCCAGTC
AvaI 1851 CTCGAG C^[CT]CG[GA]G
3071 CTCGAG
BamHI 935 GGATCC G^GATCC
3833 GGATCC
BbsI 572 GAAGAC GAAGAC(2/6)
3662 GTCTTC
BpuAI 572 GAAGAC GAAGAC(2/6)
3662 GTCTTC
BsaBI 1403 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2835 GATCTGTATC
BsoBI 1851 CTCGAG C^[CT]CG[GA]G
3071 CTCGAG
BspMI 2724 ACCTGC ACCTGC(4/8)
4138 GCAGGT
BsrDI 2437 CATTGC GCAATG(2/0)
4251 CATTGC
Bst1107I 1187 GTATAC GTA^TAC
3654 GTATAC
BstXI 174 CCAGTAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1350 CCAATGGTGTGG
BstZ17I 1187 GTATAC GTA^TAC
3654 GTATAC
EaeI 48 CGGCCA [CT]^GGCC[GA]
1519 TGGCCA
HapII 1560 CCGG C^CGG
3373 CCGG
HpaII 1560 CCGG C^CGG
3373 CCGG
MaeII 2694 ACGT A^CGT
4201 ACGT
MamI 1403 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2835 GATCTGTATC
MfeI 387 CAATTG C^AATTG
2503 CAATTG
MspI 1560 CCGG C^CGG
3373 CCGG
MunI 387 CAATTG C^AATTG
2503 CAATTG
NdeI 1200 CATATG CA^TATG
2279 CATATG
PaeR7I 1851 CTCGAG C^TCGAG
3071 CTCGAG
PflFI 185 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3299 GACCCAGTC
PshBI 986 ATTAAT AT^TAAT
1727 ATTAAT
PvuII 35 CAGCTG CAG^CTG
2847 CAGCTG
StuI 305 AGGCCT AGG^CCT
3075 AGGCCT
Tth111I 185 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3299 GACCCAGTC
XbaI 3666 TCTAGA T^CTAGA
3916 TCTAGA
XhoI 1851 CTCGAG C^TCGAG
3071 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI ApoI Asp700I AspHI
AvaII BanI BanII BbvI BclI
BfaI BfrI BglII BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI