##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccII AgeI Aor51HI ApaI
AscI AspI AvrII BbrPI BciVI
BlnI BlpI Bpu1102I BsiWI BsmI
BspDI BssHII Bst1107I BstUI BstXI
BstZ17I CelII ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I EcoRI
EcoRV FseI HpaI KspI MfeI
MluI MunI MvnI NaeI NdeI
NgoMIV NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SbfI SfiI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 4062 GTCTAC GT^[AC][GT]AC
AccIII 3637 TCCGGA T^CCGGA
AclI 1158 AACGTT AA^CGTT
AhdI 3046 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3061 GTGCAC G^TGCAC
AocI 2365 CCTTAGG CC^T[ACGT]AGG
ApaLI 3061 GTGCAC G^TGCAC
AspEI 3046 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 971 TGCGCA TGC^GCA
BamHI 1344 GGATCC G^GATCC
BanII 1729 GAGCTC G[GA]GC[CT]^C
BbeI 898 GGCGCC GGCGC^C
BcgI 1782 GCATCCCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4399 GCCAGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1457 GAGACC GGTCTC(1/5)
BseAI 3637 TCCGGA T^CCGGA
BsiEI 1224 CGACCG CG[GA][CT]^CG
BsmBI 2882 CGTCTC CGTCTC(1/5)
Bsp1407I 2596 TGTACA T^GTACA
BspEI 3637 TCCGGA T^CCGGA
BsrBI 1227 CCGCTC CCGCTC(-3/-3)
BsrGI 2596 TGTACA T^GTACA
BssSI 2900 CTCGTG CACGAG(-5/-1)
BstBI 1610 TTCGAA TT^CGAA
BstEII 1103 GGTCACC G^GT[ACGT]ACC
BstPI 1103 GGTCACC G^GT[ACGT]ACC
Bsu36I 2365 CCTTAGG CC^T[ACGT]AGG
Eam1105I 3046 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2365 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1103 GGTCACC G^GT[ACGT]ACC
EcoT22I 1780 ATGCAT ATGCA^T
FauI 2643 CCCGC CCCGC(4/6)
FspI 971 TGCGCA TGC^GCA
KasI 898 GGCGCC G^GCGCC
MroI 3637 TCCGGA T^CCGGA
NarI 898 GGCGCC GG^CGCC
NcoI 2550 CCATGG C^CATGG
NsiI 1780 ATGCAT ATGCA^T
NspV 1610 TTCGAA TT^CGAA
Psp1406I 1158 AACGTT AA^CGTT
SacI 1729 GAGCTC GAGCT^C
SapI 3018 GCTCTTC GCTCTTC(1/4)
SexAI 4329 ACCTGGT A^CC[AT]GGT
SfoI 898 GGCGCC GGC^GCC
SfuI 1610 TTCGAA TT^CGAA
SpeI 3985 ACTAGT A^CTAGT
SspBI 2596 TGTACA T^GTACA
SspI 1252 AATATT AAT^ATT
TaqI 1611 TCGA T^CGA
TthHB8I 1611 TCGA T^CGA
XbaI 768 TCTAGA T^CTAGA
XcmI 1010 CCATTGACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1852 GGTACC G^GTACC
1909 GGTACC
AcyI 745 GACGCC G[GA]^CG[CT]C
898 GGCGCC
AflII 454 CTTAAG C^TTAAG
4552 CTTAAG
Asp700I 2435 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3413 GAAAAACTTC
Asp718I 1852 GGTACC G^GTACC
1909 GGTACC
BalI 987 TGGCCA TGG^CCA
1464 TGGCCA
BcnI 210 CCGGG CC^[GC]GG
1134 CCGGG
BfrI 454 CTTAAG C^TTAAG
4552 CTTAAG
BglII 3614 AGATCT A^GATCT
4418 AGATCT
BsaAI 2271 TACGTG [CT]AC^GT[GA]
4342 TACGTG
BsaBI 405 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3281 GATAGTGATC
BsaHI 745 GACGCC G[GA]^CG[CT]C
898 GGCGCC
BsaWI 2038 TCCGGT [AT]^CCGG[AT]
3637 TCCGGA
BspHI 2500 TCATGA T^CATGA
4620 TCATGA
BspLU11I 2730 ACATGT A^CATGT
4678 ACATGT
BsrFI 647 ACCGGC [GA]^CCGG[CT]
3468 ACCGGC
Cfr10I 647 ACCGGC [GA]^CCGG[CT]
3468 ACCGGC
DraII 449 AGGACCT [GA]G^G[ACGT]CC[CT]
2639 GGGACCC
DrdI 2641 GACCCGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4624 GACTGCACAGTC
EcoO109I 449 AGGACCT [GA]G^G[ACGT]CC[CT]
2639 GGGACCC
EcoT14I 2550 CCATGG C^C[AT][AT]GG
3974 CCTTGG
HaeII 726 GGCGCT [GA]GCGC^[CT]
898 GGCGCC
HgaI 745 GACGC GACGC(5/10)
1050 GCGTC
Hin1I 745 GACGCC G[GA]^CG[CT]C
898 GGCGCC
HindIII 1548 AAGCTT A^AGCTT
2915 AAGCTT
KpnI 1852 GGTACC GGTAC^C
1909 GGTACC
MamI 405 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3281 GATAGTGATC
MluNI 987 TGGCCA TGG^CCA
1464 TGGCCA
MscI 987 TGGCCA TGG^CCA
1464 TGGCCA
MslI 1203 CACTAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3278 CATGATAGTG
NciI 210 CCGGG CC^[GC]GG
1134 CCGGG
NheI 3940 GCTAGC G^CTAGC
4053 GCTAGC
PpuMI 449 AGGACCT [GA]G^G[AT]CC[CT]
2639 GGGACCC
PstI 622 CTGCAG CTGCA^G
2055 CTGCAG
RcaI 2500 TCATGA T^CATGA
4620 TCATGA
ScaI 2288 AGTACT AGT^ACT
4385 AGTACT
StuI 199 AGGCCT AGG^CCT
1705 AGGCCT
StyI 2550 CCATGG C^C[AT][AT]GG
3974 CCTTGG
XmnI 2435 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3413 GAAAAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaI AvaII BanI BbsI
BbvCI BbvI BclI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoNI
EcoRII FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PshBI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII