##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AclI AflII AhdI Alw44I ApaLI
AscI AseI AsnI AspEI BcgI
BfrI BsiEI BsmBI BspDI BspHI
BsrBI BssHII BssSI ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRV
EcoT22I FseI HindIII HpaI MfeI
MluI MunI NheI NotI NruI
NsiI PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI ScaI SfiI SgrAI SnaBI
SphI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 3000 GACGTC GACGT^C
AcsI 3075 GAATTC [GA]^AATT[CT]
AgeI 228 ACCGGT A^CCGGT
Aor51HI 3103 AGCGCT AGC^GCT
ApoI 3075 GAATTC [GA]^AATT[CT]
Asp700I 2826 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 445 TGCGCA TGC^GCA
BbeI 62 GGCGCC GGCGC^C
BbrPI 4308 CACGTG CAC^GTG
BglI 2503 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4240 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4240 GCTCAGC GC^T[ACGT]AGC
BsaAI 4308 CACGTG [CT]AC^GT[GA]
BsaBI 2285 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 2464 CGTACG C^GTACG
BsmI 896 GCATTC GAATGC(1/-1)
Bsp1407I 4355 TGTACA T^GTACA
BspLU11I 3925 ACATGT A^CATGT
BsrGI 4355 TGTACA T^GTACA
Bst1107I 2204 GTATAC GTA^TAC
BstZ17I 2204 GTATAC GTA^TAC
CelII 4240 GCTCAGC GC^T[ACGT]AGC
DraI 4404 TTTAAA TTT^AAA
Eco47III 3103 AGCGCT AGC^GCT
EcoRI 3075 GAATTC G^AATTC
FspI 445 TGCGCA TGC^GCA
KasI 62 GGCGCC G^GCGCC
KspI 208 CCGCGG CCGC^GG
MamI 2285 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 62 GGCGCC GG^CGCC
PinAI 228 ACCGGT A^CCGGT
PmaCI 4308 CACGTG CAC^GTG
PmlI 4308 CACGTG CAC^GTG
SacII 208 CCGCGG CCGC^GG
SalI 696 GTCGAC G^TCGAC
SbfI 368 CCTGCAGG CCTGCA^GG
SexAI 3220 ACCTGGT A^CC[AT]GGT
SfoI 62 GGCGCC GGC^GCC
SmaI 718 CCCGGG CCC^GGG
SmlI 1668 CTTGAG C^T[CT][GA]AG
SpeI 813 ACTAGT A^CTAGT
Sse8387I 368 CCTGCAGG CCTGCA^GG
SspBI 4355 TGTACA T^GTACA
XbaI 3650 TCTAGA T^CTAGA
XmaCI 718 CCCGGG C^CCGGG
XmaI 718 CCCGGG C^CCGGG
XmnI 2826 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 1609 TCCGGA T^CCGGA
2197 TCCGGA
AflIII 425 ACACGT A^C[GA][CT]GT
3925 ACATGT
AocI 1005 CCTGAGG CC^T[ACGT]AGG
4060 CCTGAGG
ApaI 1722 GGGCCC GGGCC^C
4103 GGGCCC
AspHI 1194 GAGCAC G[AT]GC[AT]^C
3272 GAGCAC
AspI 519 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2574 GACCATGTC
AvrII 3260 CCTAGG C^CTAGG
3896 CCTAGG
BciVI 393 GGATAC GTATCC(6/5)
4420 GGATAC
BlnI 3260 CCTAGG C^CTAGG
3896 CCTAGG
BseAI 1609 TCCGGA T^CCGGA
2197 TCCGGA
BsiHKAI 1194 GAGCAC G[AT]GC[AT]^C
3272 GAGCAC
BspEI 1609 TCCGGA T^CCGGA
2197 TCCGGA
BsrDI 2114 CATTGC GCAATG(2/0)
4482 GCAATG
BstBI 577 TTCGAA TT^CGAA
1350 TTCGAA
Bsu36I 1005 CCTGAGG CC^T[ACGT]AGG
4060 CCTGAGG
DrdI 1413 GACCTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4175 GACTGTAGGGTC
Eco81I 1005 CCTGAGG CC^T[ACGT]AGG
4060 CCTGAGG
EcoNI 293 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3255 CCTGCCCTAGG
HincII 696 GTCGAC GT[CT]^[GA]AC
1737 GTCAAC
HindII 696 GTCGAC GT[CT]^[GA]AC
1737 GTCAAC
MroI 1609 TCCGGA T^CCGGA
2197 TCCGGA
MseI 4097 TTAA T^TAA
4405 TTAA
NaeI 838 GCCGGC GCC^GGC
4038 GCCGGC
NcoI 122 CCATGG C^CATGG
3148 CCATGG
NdeI 2015 CATATG CA^TATG
2491 CATATG
NgoMIV 838 GCCGGC G^CCGGC
4038 GCCGGC
NspV 577 TTCGAA TT^CGAA
1350 TTCGAA
PflFI 519 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2574 GACCATGTC
PflMI 641 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2806 CCATCAAGTGG
SapI 3354 GAAGAGC GCTCTTC(1/4)
1604 GCTCTTC
SfuI 577 TTCGAA TT^CGAA
1350 TTCGAA
Tru9I 4097 TTAA T^TAA
4405 TTAA
Tsp509I 324 AATT ^AATT
3076 AATT
Tth111I 519 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2574 GACCATGTC
Van91I 641 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2806 CCATCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
Acc65I AccI AccII AciI AcyI
AfaI AluI AlwI AlwNI Asp718I
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XcmI XhoII