################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AccI AccIII AclI AhdI Alw44I ApaLI AscI AspEI AspI AviII BbeI BsaBI BseAI BsmI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FseI FspI HpaI KasI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PmeI Psp1406I RsrII SalI ScaI SfoI SfuI SgrAI SnaBI SpeI SphI SspI StuI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 2714 GACGTC GACGT^C AcsI 4997 GAATTT [GA]^AATT[CT] AflII 1767 CTTAAG C^TTAAG AflIII 5023 ACATGT A^C[GA][CT]GT AgeI 1654 ACCGGT A^CCGGT ApoI 4997 GAATTT [GA]^AATT[CT] AseI 399 ATTAAT AT^TAAT AsnI 399 ATTAAT AT^TAAT Asp700I 4719 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 4549 CCTAGG C^CTAGG BbvCI 3525 GCTGAGG CCTCAGC(-5/-2) BclI 4518 TGATCA T^GATCA BfrI 1767 CTTAAG C^TTAAG BglII 997 AGATCT A^GATCT BlnI 4549 CCTAGG C^CTAGG BsiWI 961 CGTACG C^GTACG Bsp1407I 3421 TGTACA T^GTACA BspHI 4534 TCATGA T^CATGA BspLU11I 5023 ACATGT A^CATGT BsrGI 3421 TGTACA T^GTACA BstAPI 4326 GCAGACGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EagI 3535 CGGCCG C^GGCCG EclXI 3535 CGGCCG C^GGCCG Eco52I 3535 CGGCCG C^GGCCG FbaI 4518 TGATCA T^GATCA HincII 1536 GTCAAC GT[CT]^[GA]AC HindII 1536 GTCAAC GT[CT]^[GA]AC HindIII 4468 AAGCTT A^AGCTT KspI 986 CCGCGG CCGC^GG PinAI 1654 ACCGGT A^CCGGT PshAI 3545 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 399 ATTAAT AT^TAAT PvuI 3657 CGATCG CGAT^CG RcaI 4534 TCATGA T^CATGA SacII 986 CCGCGG CCGC^GG SapI 3428 GCTCTTC GCTCTTC(1/4) SbfI 5040 CCTGCAGG CCTGCA^GG SexAI 2503 ACCTGGT A^CC[AT]GGT SfiI 2967 GGCCTGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 5040 CCTGCAGG CCTGCA^GG SspBI 3421 TGTACA T^GTACA XbaI 4046 TCTAGA T^CTAGA XmnI 4719 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 165 GGTACC G^GTACC 4000 GGTACC AcyI 2714 GACGTC G[GA]^CG[CT]C 4754 GACGCC AocI 494 CCTTAGG CC^T[ACGT]AGG 3698 CCTAAGG Aor51HI 1102 AGCGCT AGC^GCT 1516 AGCGCT Asp718I 165 GGTACC G^GTACC 4000 GGTACC BbrPI 2256 CACGTG CAC^GTG 3069 CACGTG BcgI 75 CGAGGAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3359 CGAACAGGCTGC BciVI 1263 GTATCC GTATCC(6/5) 1627 GTATCC BglI 2968 GCCTGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4361 GCCCTCTGGGC BsaHI 2714 GACGTC G[GA]^CG[CT]C 4754 GACGCC BsmBI 1410 CGTCTC CGTCTC(1/5) 1667 CGTCTC BssSI 2518 CACGAG CACGAG(-5/-1) 2070 CTCGTG Bsu36I 494 CCTTAGG CC^T[ACGT]AGG 3698 CCTAAGG DraIII 1188 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG 1596 CACACGGTG DrdI 564 GACTACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3588 GACACAGTGGTC Eco47III 1102 AGCGCT AGC^GCT 1516 AGCGCT Eco81I 494 CCTTAGG CC^T[ACGT]AGG 3698 CCTAAGG Hin1I 2714 GACGTC G[GA]^CG[CT]C 4754 GACGCC KpnI 165 GGTACC GGTAC^C 4000 GGTACC NaeI 1065 GCCGGC GCC^GGC 2977 GCCGGC NcoI 1702 CCATGG C^CATGG 4337 CCATGG NgoMIV 1065 GCCGGC G^CCGGC 2977 GCCGGC NspI 2996 GCATGT [GA]CATG^[CT] 5023 ACATGT PmaCI 2256 CACGTG CAC^GTG 3069 CACGTG PmlI 2256 CACGTG CAC^GTG 3069 CACGTG SacI 824 GAGCTC GAGCT^C 1212 GAGCTC ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PaeR7I PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI