##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccI AccIII AclI AhdI Alw44I
ApaLI AscI AspEI AspI AviII
BbeI BsaBI BseAI BsmI BspDI
BspEI BsrBI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI Eam1105I
EcoRI EcoRV EcoT22I FseI FspI
HpaI KasI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PmeI Psp1406I RsrII SalI
ScaI SfoI SfuI SgrAI SnaBI
SpeI SphI SspI StuI SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2714 GACGTC GACGT^C
AcsI 4997 GAATTT [GA]^AATT[CT]
AflII 1767 CTTAAG C^TTAAG
AflIII 5023 ACATGT A^C[GA][CT]GT
AgeI 1654 ACCGGT A^CCGGT
ApoI 4997 GAATTT [GA]^AATT[CT]
AseI 399 ATTAAT AT^TAAT
AsnI 399 ATTAAT AT^TAAT
Asp700I 4719 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4549 CCTAGG C^CTAGG
BbvCI 3525 GCTGAGG CCTCAGC(-5/-2)
BclI 4518 TGATCA T^GATCA
BfrI 1767 CTTAAG C^TTAAG
BglII 997 AGATCT A^GATCT
BlnI 4549 CCTAGG C^CTAGG
BsiWI 961 CGTACG C^GTACG
Bsp1407I 3421 TGTACA T^GTACA
BspHI 4534 TCATGA T^CATGA
BspLU11I 5023 ACATGT A^CATGT
BsrGI 3421 TGTACA T^GTACA
BstAPI 4326 GCAGACGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 3535 CGGCCG C^GGCCG
EclXI 3535 CGGCCG C^GGCCG
Eco52I 3535 CGGCCG C^GGCCG
FbaI 4518 TGATCA T^GATCA
HincII 1536 GTCAAC GT[CT]^[GA]AC
HindII 1536 GTCAAC GT[CT]^[GA]AC
HindIII 4468 AAGCTT A^AGCTT
KspI 986 CCGCGG CCGC^GG
PinAI 1654 ACCGGT A^CCGGT
PshAI 3545 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 399 ATTAAT AT^TAAT
PvuI 3657 CGATCG CGAT^CG
RcaI 4534 TCATGA T^CATGA
SacII 986 CCGCGG CCGC^GG
SapI 3428 GCTCTTC GCTCTTC(1/4)
SbfI 5040 CCTGCAGG CCTGCA^GG
SexAI 2503 ACCTGGT A^CC[AT]GGT
SfiI 2967 GGCCTGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 5040 CCTGCAGG CCTGCA^GG
SspBI 3421 TGTACA T^GTACA
XbaI 4046 TCTAGA T^CTAGA
XmnI 4719 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 165 GGTACC G^GTACC
4000 GGTACC
AcyI 2714 GACGTC G[GA]^CG[CT]C
4754 GACGCC
AocI 494 CCTTAGG CC^T[ACGT]AGG
3698 CCTAAGG
Aor51HI 1102 AGCGCT AGC^GCT
1516 AGCGCT
Asp718I 165 GGTACC G^GTACC
4000 GGTACC
BbrPI 2256 CACGTG CAC^GTG
3069 CACGTG
BcgI 75 CGAGGAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3359 CGAACAGGCTGC
BciVI 1263 GTATCC GTATCC(6/5)
1627 GTATCC
BglI 2968 GCCTGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4361 GCCCTCTGGGC
BsaHI 2714 GACGTC G[GA]^CG[CT]C
4754 GACGCC
BsmBI 1410 CGTCTC CGTCTC(1/5)
1667 CGTCTC
BssSI 2518 CACGAG CACGAG(-5/-1)
2070 CTCGTG
Bsu36I 494 CCTTAGG CC^T[ACGT]AGG
3698 CCTAAGG
DraIII 1188 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1596 CACACGGTG
DrdI 564 GACTACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3588 GACACAGTGGTC
Eco47III 1102 AGCGCT AGC^GCT
1516 AGCGCT
Eco81I 494 CCTTAGG CC^T[ACGT]AGG
3698 CCTAAGG
Hin1I 2714 GACGTC G[GA]^CG[CT]C
4754 GACGCC
KpnI 165 GGTACC GGTAC^C
4000 GGTACC
NaeI 1065 GCCGGC GCC^GGC
2977 GCCGGC
NcoI 1702 CCATGG C^CATGG
4337 CCATGG
NgoMIV 1065 GCCGGC G^CCGGC
2977 GCCGGC
NspI 2996 GCATGT [GA]CATG^[CT]
5023 ACATGT
PmaCI 2256 CACGTG CAC^GTG
3069 CACGTG
PmlI 2256 CACGTG CAC^GTG
3069 CACGTG
SacI 824 GAGCTC GAGCT^C
1212 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI