##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AclI AflIII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BglII
BlnI BsaBI BsiWI BspDI BspHI
BspLU11I BssSI BstBI BstYI ClaI
CpoI DraIII DrdI Eam1105I EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MflI MluI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SexAI SfuI SnaBI SpeI Sse8387I
SwaI Tth111I XhoI XhoII
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 3490 GACGTC GACGT^C
AflII 2926 CTTAAG C^TTAAG
Aor51HI 576 AGCGCT AGC^GCT
BalI 658 TGGCCA TGG^CCA
BbsI 2312 GAAGAC GAAGAC(2/6)
BciVI 3120 GTATCC GTATCC(6/5)
BfrI 2926 CTTAAG C^TTAAG
BpuAI 2312 GAAGAC GAAGAC(2/6)
BsaAI 2842 TACGTG [CT]AC^GT[GA]
BsaI 669 GAGACC GGTCTC(1/5)
BsmBI 3488 GAGACG CGTCTC(1/5)
BsmI 2894 GAATGC GAATGC(1/-1)
BspMI 2223 ACCTGC ACCTGC(4/8)
BssHII 2028 GCGCGC G^CGCGC
BstAPI 1328 GCACTAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1072 GGTCACC G^GT[ACGT]ACC
BstPI 1072 GGTCACC G^GT[ACGT]ACC
EagI 403 CGGCCG C^GGCCG
EclXI 403 CGGCCG C^GGCCG
Eco47III 576 AGCGCT AGC^GCT
Eco52I 403 CGGCCG C^GGCCG
EcoNI 421 CCTCGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1072 GGTCACC G^GT[ACGT]ACC
EcoRI 2325 GAATTC G^AATTC
MfeI 1059 CAATTG C^AATTG
MluNI 658 TGGCCA TGG^CCA
MscI 658 TGGCCA TGG^CCA
MunI 1059 CAATTG C^AATTG
NcoI 515 CCATGG C^CATGG
NdeI 705 CATATG CA^TATG
PpuMI 2220 AGGACCT [GA]G^G[AT]CC[CT]
PstI 3308 CTGCAG CTGCA^G
SfiI 242 GGCCGGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 503 CGCCGGTG C[GA]^CCGG[CT]G
StuI 10 AGGCCT AGG^CCT
XbaI 1627 TCTAGA T^CTAGA
XcmI 2869 CCAAAGTGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 190 TCCGGA T^CCGGA
1704 TCCGGA
AocI 334 CCTGAGG CC^T[ACGT]AGG
3495 CCTTAGG
ApaI 288 GGGCCC GGGCC^C
398 GGGCCC
Asp700I 3225 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3277 GAACAACTTC
BanI 88 GGCGCC G^G[CT][GA]CC
330 GGCGCC
BbeI 88 GGCGCC GGCGC^C
330 GGCGCC
BfaI 1212 CTAG C^TAG
1628 CTAG
BlpI 1756 GCTGAGC GC^T[ACGT]AGC
2703 GCTTAGC
Bpu1102I 1756 GCTGAGC GC^T[ACGT]AGC
2703 GCTTAGC
BseAI 190 TCCGGA T^CCGGA
1704 TCCGGA
BsiEI 97 CGGTCG CG[GA][CT]^CG
403 CGGCCG
Bsp1407I 1991 TGTACA T^GTACA
2908 TGTACA
BspEI 190 TCCGGA T^CCGGA
1704 TCCGGA
BsrDI 957 CATTGC GCAATG(2/0)
2699 CATTGC
BsrGI 1991 TGTACA T^GTACA
2908 TGTACA
Bst1107I 1554 GTATAC GTA^TAC
2174 GTATAC
BstXI 877 CCAAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2628 CCAGTGAGATGG
BstZ17I 1554 GTATAC GTA^TAC
2174 GTATAC
Bsu36I 334 CCTGAGG CC^T[ACGT]AGG
3495 CCTTAGG
CelII 1756 GCTGAGC GC^T[ACGT]AGC
2703 GCTTAGC
DsaI 355 CCGTGG C^C[GA][CT]GG
515 CCATGG
Eco81I 334 CCTGAGG CC^T[ACGT]AGG
3495 CCTTAGG
EcoT14I 515 CCATGG C^C[AT][AT]GG
661 CCAAGG
KasI 88 GGCGCC G^GCGCC
330 GGCGCC
MaeI 1212 CTAG C^TAG
1628 CTAG
MroI 190 TCCGGA T^CCGGA
1704 TCCGGA
NaeI 243 GCCGGC GCC^GGC
405 GCCGGC
NarI 88 GGCGCC GG^CGCC
330 GGCGCC
NgoMIV 243 GCCGGC G^CCGGC
405 GCCGGC
PflMI 350 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
839 CCAAAATGTGG
ScaI 3340 AGTACT AGT^ACT
3804 AGTACT
SfoI 88 GGCGCC GGC^GCC
330 GGCGCC
SmaI 145 CCCGGG CCC^GGG
291 CCCGGG
SmlI 119 CTCAAG C^T[CT][GA]AG
2926 CTTAAG
SphI 1286 GCATGC GCATG^C
1761 GCATGC
SspBI 1991 TGTACA T^GTACA
2908 TGTACA
SspI 831 AATATT AAT^ATT
3839 AATATT
StyI 515 CCATGG C^C[AT][AT]GG
661 CCAAGG
Van91I 350 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
839 CCAAAATGTGG
XmaCI 145 CCCGGG C^CCGGG
291 CCCGGG
XmaI 145 CCCGGG C^CCGGG
291 CCCGGG
XmnI 3225 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3277 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanII BbvI
BclI BcnI BglI BmyI BpmI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I