##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
ApaI ApaLI AscI Asp718I AspI
AvrII BbeI BbrPI BbvCI BciVI
BglII BlnI BsaBI BseAI BsiWI
BsmI BspEI BssHII BstEII BstPI
DrdI EcoO65I EcoRI EcoRV FseI
HindIII KasI KpnI KspI MamI
MluI MroI NarI NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI SacII SalI SbfI ScaI
SfiI SfoI SgrAI SnaBI SphI
Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 69 enzymes) #
###################################
AatII 208 GACGTC GACGT^C
AccI 1613 GTATAC GT^[AC][GT]AC
AccII 232 CGCG CG^CG
AflII 1096 CTTAAG C^TTAAG
Aor51HI 3277 AGCGCT AGC^GCT
AseI 1224 ATTAAT AT^TAAT
AsnI 1224 ATTAAT AT^TAAT
AviII 789 TGCGCA TGC^GCA
BalI 290 TGGCCA TGG^CCA
BanI 3491 GGTGCC G^G[CT][GA]CC
BcgI 2918 GCAGGAGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 495 TGATCA T^GATCA
BfrI 1096 CTTAAG C^TTAAG
BglI 3494 GCCCTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1978 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1978 GCTCAGC GC^T[ACGT]AGC
BsaAI 3972 TACGTG [CT]AC^GT[GA]
BsaI 92 GGTCTC GGTCTC(1/5)
BsaWI 2488 TCCGGT [AT]^CCGG[AT]
Bsp1407I 3717 TGTACA T^GTACA
BspDI 879 ATCGAT AT^CGAT
BspLU11I 3794 ACATGT A^CATGT
BsrGI 3717 TGTACA T^GTACA
BssSI 3208 CACGAG CACGAG(-5/-1)
Bst1107I 1613 GTATAC GTA^TAC
BstAPI 1903 GCATGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3433 TTCGAA TT^CGAA
BstUI 232 CGCG CG^CG
BstXI 3599 CCATTGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1613 GTATAC GTA^TAC
CelII 1978 GCTCAGC GC^T[ACGT]AGC
ClaI 879 ATCGAT AT^CGAT
CpoI 2976 CGGTCCG CG^G[AT]CCG
DraIII 2396 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3277 AGCGCT AGC^GCT
EcoT22I 1916 ATGCAT ATGCA^T
FbaI 495 TGATCA T^GATCA
FspI 789 TGCGCA TGC^GCA
HincII 508 GTTAAC GT[CT]^[GA]AC
HindII 508 GTTAAC GT[CT]^[GA]AC
HpaI 508 GTTAAC GTT^AAC
MfeI 2408 CAATTG C^AATTG
MluNI 290 TGGCCA TGG^CCA
MscI 290 TGGCCA TGG^CCA
MunI 2408 CAATTG C^AATTG
MvnI 232 CGCG CG^CG
NaeI 262 GCCGGC GCC^GGC
NdeI 3289 CATATG CA^TATG
NgoMIV 262 GCCGGC G^CCGGC
NsiI 1916 ATGCAT ATGCA^T
NspV 3433 TTCGAA TT^CGAA
PflMI 3951 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2964 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1224 ATTAAT AT^TAAT
PstI 3553 CTGCAG CTGCA^G
RsrII 2976 CGGTCCG CG^G[AT]CCG
SapI 62 GCTCTTC GCTCTTC(1/4)
SexAI 641 ACCTGGT A^CC[AT]GGT
SfuI 3433 TTCGAA TT^CGAA
SmaI 87 CCCGGG CCC^GGG
SpeI 1254 ACTAGT A^CTAGT
SspBI 3717 TGTACA T^GTACA
SspI 1658 AATATT AAT^ATT
StuI 2753 AGGCCT AGG^CCT
Van91I 3951 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 774 TCTAGA T^CTAGA
XcmI 2851 CCAAACGGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 87 CCCGGG C^CCGGG
XmaI 87 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwNI 307 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2740 CAGGGACTG
Asp700I 1310 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2601 GAAGAAATTC
BbsI 732 GTCTTC GAAGAC(2/6)
2428 GTCTTC
BpuAI 732 GTCTTC GAAGAC(2/6)
2428 GTCTTC
BsiEI 20 CGGCCG CG[GA][CT]^CG
727 CGGCCG
BspHI 1565 TCATGA T^CATGA
1580 TCATGA
BsrFI 262 GCCGGC [GA]^CCGG[CT]
3202 GCCGGT
Cfr10I 262 GCCGGC [GA]^CCGG[CT]
3202 GCCGGT
EagI 20 CGGCCG C^GGCCG
727 CGGCCG
EclXI 20 CGGCCG C^GGCCG
727 CGGCCG
Eco52I 20 CGGCCG C^GGCCG
727 CGGCCG
HaeII 2847 AGCGCC [GA]GCGC^[CT]
3277 AGCGCT
PvuII 1381 CAGCTG CAG^CTG
3955 CAGCTG
RcaI 1565 TCATGA T^CATGA
1580 TCATGA
SacI 247 GAGCTC GAGCT^C
3182 GAGCTC
XmnI 1310 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2601 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AhdI AluI AlwI AocI ApoI
AspEI AspHI AvaI AvaII BamHI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII