##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccI AclI AgeI Aor51HI
AscI AseI AsnI Asp718I AspI
AviII BamHI BbeI BcgI BclI
BglI BglII BsiWI Bsp1407I BspDI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 970 GAATTT [GA]^AATT[CT]
AflII 2579 CTTAAG C^TTAAG
AhdI 1531 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 557 GTGCAC G^TGCAC
ApaLI 557 GTGCAC G^TGCAC
ApoI 970 GAATTT [GA]^AATT[CT]
AspEI 1531 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2328 CACGTG CAC^GTG
BbsI 987 GTCTTC GAAGAC(2/6)
BciVI 2898 GTATCC GTATCC(6/5)
BfrI 2579 CTTAAG C^TTAAG
BpuAI 987 GTCTTC GAAGAC(2/6)
BsaAI 2328 CACGTG [CT]AC^GT[GA]
BsgI 1245 GTGCAG GTGCAG(16/14)
BsiEI 1058 CGACCG CG[GA][CT]^CG
BsmBI 2492 GAGACG CGTCTC(1/5)
BspHI 3511 TCATGA T^CATGA
BsrFI 1479 ACCGGC [GA]^CCGG[CT]
Cfr10I 1479 ACCGGC [GA]^CCGG[CT]
DraI 3936 TTTAAA TTT^AAA
Eam1105I 1531 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 419 GATATC GAT^ATC
NheI 856 GCTAGC G^CTAGC
PaeR7I 1808 CTCGAG C^TCGAG
PflMI 3740 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2328 CACGTG CAC^GTG
PmlI 2328 CACGTG CAC^GTG
RcaI 3511 TCATGA T^CATGA
SbfI 571 CCTGCAGG CCTGCA^GG
SexAI 3769 ACCTGGT A^CC[AT]GGT
SphI 3108 GCATGC GCATG^C
Sse8387I 571 CCTGCAGG CCTGCA^GG
SspI 3655 AATATT AAT^ATT
Van91I 3740 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1808 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 1722 GACGTC GACGT^C
3894 GACGTC
AccII 2422 CGCG CG^CG
2511 CGCG
AflIII 2327 ACACGT A^C[GA][CT]GT
2495 ACGTGT
Asp700I 280 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2479 GAAGCAGTTC
AvrII 3671 CCTAGG C^CTAGG
4051 CCTAGG
BlnI 3671 CCTAGG C^CTAGG
4051 CCTAGG
BsaBI 175 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2764 GATCAAGATC
BsmI 889 GAATGC GAATGC(1/-1)
2991 GCATTC
BsrDI 913 CATTGC GCAATG(2/0)
893 GCAATG
BstAPI 893 GCAATGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1886 GCACGGGCTGC
BstUI 2422 CGCG CG^CG
2511 CGCG
DraIII 3183 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3195 CACCCCGTG
HincII 977 GTTGAC GT[CT]^[GA]AC
1325 GTCAAC
HindII 977 GTTGAC GT[CT]^[GA]AC
1325 GTCAAC
MamI 175 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2764 GATCAAGATC
MvnI 2422 CGCG CG^CG
2511 CGCG
NspI 1290 GCATGT [GA]CATG^[CT]
3108 GCATGC
PshAI 948 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
980 GACTCCTGTC
ScaI 960 AGTACT AGT^ACT
2136 AGTACT
SmaI 25 CCCGGG CCC^GGG
339 CCCGGG
StuI 2937 AGGCCT AGG^CCT
3821 AGGCCT
TfiI 926 GAATC G^A[AT]TC
1412 GAATC
XmaCI 25 CCCGGG C^CCGGG
339 CCCGGG
XmaI 25 CCCGGG C^CCGGG
339 CCCGGG
XmnI 280 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2479 GAAGCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccIII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII