##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AhdI Aor51HI AscI AseI
AsnI Asp700I AspEI AvrII BamHI
BbeI BbrPI BcgI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiWI BspEI BspHI BssHII Bst1107I
BstBI BstUI BstZ17I CelII CpoI
DrdI Eam1105I Eco47III EcoRV EcoT22I
FbaI FseI HpaI KasI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SmaI SnaBI SphI Sse8387I
SspI SwaI XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 3221 GGTACC G^GTACC
AcyI 3959 GGCGTC G[GA]^CG[CT]C
AflIII 1421 ACATGT A^C[GA][CT]GT
AgeI 4267 ACCGGT A^CCGGT
ApaI 1376 GGGCCC GGGCC^C
Asp718I 3221 GGTACC G^GTACC
BanI 3221 GGTACC G^G[CT][GA]CC
BglII 1388 AGATCT A^GATCT
BsaAI 2931 TACGTG [CT]AC^GT[GA]
BsaHI 3959 GGCGTC G[GA]^CG[CT]C
BsiEI 1354 CGGCCG CG[GA][CT]^CG
BsmBI 797 GAGACG CGTCTC(1/5)
Bsp1407I 1554 TGTACA T^GTACA
BspDI 574 ATCGAT AT^CGAT
BspLU11I 1421 ACATGT A^CATGT
BspMI 3224 ACCTGC ACCTGC(4/8)
BsrBI 697 GAGCGG CCGCTC(-3/-3)
BsrFI 4267 ACCGGT [GA]^CCGG[CT]
BsrGI 1554 TGTACA T^GTACA
Cfr10I 4267 ACCGGT [GA]^CCGG[CT]
ClaI 574 ATCGAT AT^CGAT
EagI 1354 CGGCCG C^GGCCG
EclXI 1354 CGGCCG C^GGCCG
Eco52I 1354 CGGCCG C^GGCCG
EcoRI 161 GAATTC G^AATTC
HaeII 3035 GGCGCT [GA]GCGC^[CT]
Hin1I 3959 GGCGTC G[GA]^CG[CT]C
HincII 3948 GTTGAC GT[CT]^[GA]AC
HindII 3948 GTTGAC GT[CT]^[GA]AC
HindIII 1051 AAGCTT A^AGCTT
KpnI 3221 GGTACC GGTAC^C
NcoI 2833 CCATGG C^CATGG
PinAI 4267 ACCGGT A^CCGGT
PshAI 1015 GACAACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2849 GCTCTTC GCTCTTC(1/4)
ScaI 344 AGTACT AGT^ACT
SexAI 3804 ACCAGGT A^CC[AT]GGT
SgrAI 4266 CACCGGTG C[GA]^CCGG[CT]G
SpeI 954 ACTAGT A^CTAGT
SspBI 1554 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AocI 369 CCTGAGG CC^T[ACGT]AGG
1411 CCTCAGG
AviII 2339 TGCGCA TGC^GCA
3104 TGCGCA
BbvCI 4283 CCTCAGC CCTCAGC(-5/-2)
1009 GCTGAGG
BsrDI 2399 CATTGC GCAATG(2/0)
986 GCAATG
BssSI 2082 CACGAG CACGAG(-5/-1)
227 CTCGTG
BstEII 854 GGTCACC G^GT[ACGT]ACC
2559 GGTGACC
BstPI 854 GGTCACC G^GT[ACGT]ACC
2559 GGTGACC
Bsu36I 369 CCTGAGG CC^T[ACGT]AGG
1411 CCTCAGG
DraIII 2062 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3139 CACCCTGTG
Eco81I 369 CCTGAGG CC^T[ACGT]AGG
1411 CCTCAGG
EcoO65I 854 GGTCACC G^GT[ACGT]ACC
2559 GGTGACC
FspI 2339 TGCGCA TGC^GCA
3104 TGCGCA
MaeII 2932 ACGT A^CGT
3438 ACGT
PflMI 612 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1764 CCAAGCTCTGG
PvuII 334 CAGCTG CAG^CTG
1912 CAGCTG
StuI 2019 AGGCCT AGG^CCT
2301 AGGCCT
Van91I 612 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1764 CCAAGCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AspI AvaI AvaII BalI BanII
BbsI BbvI BciVI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII