##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
Aor51HI ApaI AscI AseI AsnI
AspI AvaI AvrII BbeI BcgI
BglI BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BssHII
BstAPI BstBI BstEII BstPI BstUI
CelII ClaI CpoI DrdI Eco47III
EcoO65I EcoRV FseI HaeII HpaI
KasI KspI MamI MluI MroI
MvnI NarI NdeI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SwaI Tth111I Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AcyI 1593 GACGCC G[GA]^CG[CT]C
AflII 2028 CTTAAG C^TTAAG
AhdI 2779 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3048 GTGCAC G^TGCAC
AocI 986 CCTGAGG CC^T[ACGT]AGG
ApaLI 3048 GTGCAC G^TGCAC
AspEI 2779 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 94 TGCGCA TGC^GCA
BamHI 2370 GGATCC G^GATCC
BciVI 1402 GGATAC GTATCC(6/5)
BclI 395 TGATCA T^GATCA
BfrI 2028 CTTAAG C^TTAAG
BglII 4064 AGATCT A^GATCT
BsaHI 1593 GACGCC G[GA]^CG[CT]C
BsgI 3947 GTGCAG GTGCAG(16/14)
BsiEI 305 CGGCCG CG[GA][CT]^CG
BsmAI 1640 GTCTC GTCTC(1/5)
BspLU11I 2982 ACATGT A^CATGT
BspMI 1363 ACCTGC ACCTGC(4/8)
BsrDI 2912 CATTGC GCAATG(2/0)
BsrFI 2820 GCCGGC [GA]^CCGG[CT]
BssSI 2315 CACGAG CACGAG(-5/-1)
Bst1107I 3788 GTATAC GTA^TAC
BstXI 4028 CCAGTGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3788 GTATAC GTA^TAC
Bsu36I 986 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2820 GCCGGC [GA]^CCGG[CT]
DraII 3172 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 3873 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 566 CCATGG C^C[GA][CT]GG
EagI 305 CGGCCG C^GGCCG
Eam1105I 2779 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 305 CGGCCG C^GGCCG
Eco52I 305 CGGCCG C^GGCCG
Eco81I 986 CCTGAGG CC^T[ACGT]AGG
EcoO109I 3172 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 3093 GAATTC G^AATTC
EcoT22I 4347 ATGCAT ATGCA^T
FbaI 395 TGATCA T^GATCA
FspI 94 TGCGCA TGC^GCA
Hin1I 1593 GACGCC G[GA]^CG[CT]C
HincII 325 GTCGAC GT[CT]^[GA]AC
HindII 325 GTCGAC GT[CT]^[GA]AC
MfeI 52 CAATTG C^AATTG
MunI 52 CAATTG C^AATTG
NaeI 2820 GCCGGC GCC^GGC
NcoI 566 CCATGG C^CATGG
NgoMIV 2820 GCCGGC G^CCGGC
NsiI 4347 ATGCAT ATGCA^T
SalI 325 GTCGAC G^TCGAC
SbfI 1364 CCTGCAGG CCTGCA^GG
SexAI 2753 ACCAGGT A^CC[AT]GGT
SpeI 819 ACTAGT A^CTAGT
SphI 891 GCATGC GCATG^C
Sse8387I 1364 CCTGCAGG CCTGCA^GG
StuI 736 AGGCCT AGG^CCT
XbaI 2813 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 2371 GATCC GGATC(4/5)
2370 GGATC
BbrPI 3983 CACGTG CAC^GTG
4462 CACGTG
BbvCI 2324 CCTCAGC CCTCAGC(-5/-2)
3887 CCTCAGC
BcnI 553 CCCGG CC^[GC]GG
4173 CCGGG
Bsp1407I 2450 TGTACA T^GTACA
2797 TGTACA
BsrGI 2450 TGTACA T^GTACA
2797 TGTACA
BstYI 2370 GGATCC [GA]^GATC[CT]
4064 AGATCT
EcoNI 1628 CCTTGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2420 CCTCAGTAAGG
FauI 992 GCGGG CCCGC(4/6)
1994 GCGGG
MflI 2370 GGATCC [GA]^GATC[CT]
4064 AGATCT
NciI 553 CCCGG CC^[GC]GG
4173 CCGGG
NheI 1736 GCTAGC G^CTAGC
3767 GCTAGC
PmaCI 3983 CACGTG CAC^GTG
4462 CACGTG
PmlI 3983 CACGTG CAC^GTG
4462 CACGTG
SapI 148 GAAGAGC GCTCTTC(1/4)
2022 GAAGAGC
SspBI 2450 TGTACA T^GTACA
2797 TGTACA
XcmI 3151 CCAATACATACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3269 CCACTGAGCTATTGG
XhoII 2370 GGATCC [GA]^GATC[CT]
4064 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AflIII AluI AlwNI ApoI Asp700I
Asp718I AspHI AvaII BalI BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsmI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI