################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII AccII AccIII AclI AgeI Aor51HI ApaI AscI AseI AsnI AspI AvaI AvrII BbeI BcgI BglI BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsoBI BspDI BspEI BspHI BssHII BstAPI BstBI BstEII BstPI BstUI CelII ClaI CpoI DrdI Eco47III EcoO65I EcoRV FseI HaeII HpaI KasI KspI MamI MluI MroI MvnI NarI NdeI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcyI 1593 GACGCC G[GA]^CG[CT]C AflII 2028 CTTAAG C^TTAAG AhdI 2779 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3048 GTGCAC G^TGCAC AocI 986 CCTGAGG CC^T[ACGT]AGG ApaLI 3048 GTGCAC G^TGCAC AspEI 2779 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 94 TGCGCA TGC^GCA BamHI 2370 GGATCC G^GATCC BciVI 1402 GGATAC GTATCC(6/5) BclI 395 TGATCA T^GATCA BfrI 2028 CTTAAG C^TTAAG BglII 4064 AGATCT A^GATCT BsaHI 1593 GACGCC G[GA]^CG[CT]C BsgI 3947 GTGCAG GTGCAG(16/14) BsiEI 305 CGGCCG CG[GA][CT]^CG BsmAI 1640 GTCTC GTCTC(1/5) BspLU11I 2982 ACATGT A^CATGT BspMI 1363 ACCTGC ACCTGC(4/8) BsrDI 2912 CATTGC GCAATG(2/0) BsrFI 2820 GCCGGC [GA]^CCGG[CT] BssSI 2315 CACGAG CACGAG(-5/-1) Bst1107I 3788 GTATAC GTA^TAC BstXI 4028 CCAGTGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3788 GTATAC GTA^TAC Bsu36I 986 CCTGAGG CC^T[ACGT]AGG Cfr10I 2820 GCCGGC [GA]^CCGG[CT] DraII 3172 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 3873 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 566 CCATGG C^C[GA][CT]GG EagI 305 CGGCCG C^GGCCG Eam1105I 2779 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 305 CGGCCG C^GGCCG Eco52I 305 CGGCCG C^GGCCG Eco81I 986 CCTGAGG CC^T[ACGT]AGG EcoO109I 3172 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 3093 GAATTC G^AATTC EcoT22I 4347 ATGCAT ATGCA^T FbaI 395 TGATCA T^GATCA FspI 94 TGCGCA TGC^GCA Hin1I 1593 GACGCC G[GA]^CG[CT]C HincII 325 GTCGAC GT[CT]^[GA]AC HindII 325 GTCGAC GT[CT]^[GA]AC MfeI 52 CAATTG C^AATTG MunI 52 CAATTG C^AATTG NaeI 2820 GCCGGC GCC^GGC NcoI 566 CCATGG C^CATGG NgoMIV 2820 GCCGGC G^CCGGC NsiI 4347 ATGCAT ATGCA^T SalI 325 GTCGAC G^TCGAC SbfI 1364 CCTGCAGG CCTGCA^GG SexAI 2753 ACCAGGT A^CC[AT]GGT SpeI 819 ACTAGT A^CTAGT SphI 891 GCATGC GCATG^C Sse8387I 1364 CCTGCAGG CCTGCA^GG StuI 736 AGGCCT AGG^CCT XbaI 2813 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 2371 GATCC GGATC(4/5) 2370 GGATC BbrPI 3983 CACGTG CAC^GTG 4462 CACGTG BbvCI 2324 CCTCAGC CCTCAGC(-5/-2) 3887 CCTCAGC BcnI 553 CCCGG CC^[GC]GG 4173 CCGGG Bsp1407I 2450 TGTACA T^GTACA 2797 TGTACA BsrGI 2450 TGTACA T^GTACA 2797 TGTACA BstYI 2370 GGATCC [GA]^GATC[CT] 4064 AGATCT EcoNI 1628 CCTTGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2420 CCTCAGTAAGG FauI 992 GCGGG CCCGC(4/6) 1994 GCGGG MflI 2370 GGATCC [GA]^GATC[CT] 4064 AGATCT NciI 553 CCCGG CC^[GC]GG 4173 CCGGG NheI 1736 GCTAGC G^CTAGC 3767 GCTAGC PmaCI 3983 CACGTG CAC^GTG 4462 CACGTG PmlI 3983 CACGTG CAC^GTG 4462 CACGTG SapI 148 GAAGAGC GCTCTTC(1/4) 2022 GAAGAGC SspBI 2450 TGTACA T^GTACA 2797 TGTACA XcmI 3151 CCAATACATACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3269 CCACTGAGCTATTGG XhoII 2370 GGATCC [GA]^GATC[CT] 4064 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### Acc65I AccI AciI AcsI AfaI AflIII AluI AlwNI ApoI Asp700I Asp718I AspHI AvaII BalI BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsmI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI