##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccI AccIII AgeI AocI
ApaI AscI AseI AsnI AvrII
BcgI BciVI BclI BglI BlnI
BseAI BsiWI BspDI BspEI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HpaI
MfeI MluI MroI MunI NheI
NruI NsiI NspV PaeR7I PinAI
PmeI PshAI PshBI PvuI RsrII
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1521 GGTACC G^GTACC
AclI 2827 AACGTT AA^CGTT
AhdI 928 GACTATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1899 AGCGCT AGC^GCT
Asp718I 1521 GGTACC G^GTACC
AspEI 928 GACTATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 286 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 606 TGCGCA TGC^GCA
BalI 576 TGGCCA TGG^CCA
BamHI 4128 GGATCC G^GATCC
BglII 834 AGATCT A^GATCT
Bsp1407I 1664 TGTACA T^GTACA
BspLU11I 3027 ACATGT A^CATGT
BsrGI 1664 TGTACA T^GTACA
BstAPI 987 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 1543 CGGCCG C^GGCCG
Eam1105I 928 GACTATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1543 CGGCCG C^GGCCG
Eco47III 1899 AGCGCT AGC^GCT
Eco52I 1543 CGGCCG C^GGCCG
EcoNI 83 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 606 TGCGCA TGC^GCA
HincII 2945 GTCAAC GT[CT]^[GA]AC
HindII 2945 GTCAAC GT[CT]^[GA]AC
HindIII 3714 AAGCTT A^AGCTT
KpnI 1521 GGTACC GGTAC^C
MluNI 576 TGGCCA TGG^CCA
MscI 576 TGGCCA TGG^CCA
NaeI 1050 GCCGGC GCC^GGC
NgoMIV 1050 GCCGGC G^CCGGC
NotI 1542 GCGGCCGC GC^GGCCGC
PacI 2999 TTAATTAA TTAAT^TAA
PflFI 286 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2827 AACGTT AA^CGTT
PvuII 2301 CAGCTG CAG^CTG
SapI 2650 GAAGAGC GCTCTTC(1/4)
SexAI 1416 ACCTGGT A^CC[AT]GGT
SphI 603 GCATGC GCATG^C
SspBI 1664 TGTACA T^GTACA
StuI 1239 AGGCCT AGG^CCT
Tth111I 286 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4513 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 1107 GGCGCC G[GA]^CG[CT]C
2915 GGCGCC
AflII 1673 CTTAAG C^TTAAG
3421 CTTAAG
Alw44I 873 GTGCAC G^TGCAC
3060 GTGCAC
ApaLI 873 GTGCAC G^TGCAC
3060 GTGCAC
Asp700I 672 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1738 GAACTGCTTC
BbeI 1107 GGCGCC GGCGC^C
2915 GGCGCC
BbrPI 876 CACGTG CAC^GTG
2866 CACGTG
BbsI 3238 GTCTTC GAAGAC(2/6)
3598 GTCTTC
BbvCI 1525 CCTCAGC CCTCAGC(-5/-2)
2187 CCTCAGC
BfrI 1673 CTTAAG C^TTAAG
3421 CTTAAG
BpuAI 3238 GTCTTC GAAGAC(2/6)
3598 GTCTTC
BsaAI 876 CACGTG [CT]AC^GT[GA]
2866 CACGTG
BsaBI 1276 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3959 GATTAGAATC
BsaHI 1107 GGCGCC G[GA]^CG[CT]C
2915 GGCGCC
BsiEI 304 CGGTCG CG[GA][CT]^CG
1543 CGGCCG
BsmBI 517 CGTCTC CGTCTC(1/5)
935 CGTCTC
BspHI 2084 TCATGA T^CATGA
3549 TCATGA
BspMI 1217 ACCTGC ACCTGC(4/8)
1827 ACCTGC
BsrBI 152 GAGCGG CCGCTC(-3/-3)
281 GAGCGG
BsrFI 1050 GCCGGC [GA]^CCGG[CT]
1787 ACCGGC
Cfr10I 1050 GCCGGC [GA]^CCGG[CT]
1787 ACCGGC
Hin1I 1107 GGCGCC G[GA]^CG[CT]C
2915 GGCGCC
KasI 1107 GGCGCC G^GCGCC
2915 GGCGCC
KspI 31 CCGCGG CCGC^GG
120 CCGCGG
MamI 1276 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3959 GATTAGAATC
NarI 1107 GGCGCC GG^CGCC
2915 GGCGCC
NdeI 2472 CATATG CA^TATG
4238 CATATG
PflMI 820 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1636 CCATGGTGTGG
PmaCI 876 CACGTG CAC^GTG
2866 CACGTG
PmlI 876 CACGTG CAC^GTG
2866 CACGTG
PpuMI 135 GGGACCC [GA]G^G[AT]CC[CT]
909 GGGACCC
RcaI 2084 TCATGA T^CATGA
3549 TCATGA
SacI 231 GAGCTC GAGCT^C
2653 GAGCTC
SacII 31 CCGCGG CCGC^GG
120 CCGCGG
ScaI 976 AGTACT AGT^ACT
3583 AGTACT
SfoI 1107 GGCGCC GGC^GCC
2915 GGCGCC
SspI 3287 AATATT AAT^ATT
3376 AATATT
Van91I 820 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1636 CCATGGTGTGG
XcmI 2389 CCAAGCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3356 CCACCACAGTCTTGG
XmnI 672 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1738 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI