##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AviII BbeI BbrPI BciVI
BclI BfrI BsaAI BsiWI BspLU11I
BsrDI BssHII Bst1107I BstAPI BstZ17I
CpoI Eco47III EcoRV FbaI FseI
FspI HaeII HpaI KasI KspI
MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI PacI
PinAI PmaCI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 72 enzymes) #
###################################
Acc65I 1703 GGTACC G^GTACC
AccII 3164 CGCG CG^CG
AccIII 78 TCCGGA T^CCGGA
AhdI 2048 GACACTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 4046 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1703 GGTACC G^GTACC
AspEI 2048 GACACTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1393 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4040 CCTAGG C^CTAGG
BalI 2884 TGGCCA TGG^CCA
BbvCI 1270 CCTCAGC CCTCAGC(-5/-2)
BcgI 1449 CGAACCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 83 AGATCT A^GATCT
BlnI 4040 CCTAGG C^CTAGG
BlpI 3809 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3809 GCTGAGC GC^T[ACGT]AGC
BsaBI 4158 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 78 TCCGGA T^CCGGA
BsmI 1365 GCATTC GAATGC(1/-1)
Bsp1407I 399 TGTACA T^GTACA
BspDI 1964 ATCGAT AT^CGAT
BspEI 78 TCCGGA T^CCGGA
BspHI 1203 TCATGA T^CATGA
BsrFI 847 ACCGGC [GA]^CCGG[CT]
BsrGI 399 TGTACA T^GTACA
BstBI 3523 TTCGAA TT^CGAA
BstEII 2586 GGTCACC G^GT[ACGT]ACC
BstPI 2586 GGTCACC G^GT[ACGT]ACC
BstUI 3164 CGCG CG^CG
CelII 3809 GCTGAGC GC^T[ACGT]AGC
CfoI 3163 GCGC GCG^C
Cfr10I 847 ACCGGC [GA]^CCGG[CT]
ClaI 1964 ATCGAT AT^CGAT
DraIII 1044 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3994 CGGCCG C^GGCCG
Eam1105I 2048 GACACTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3994 CGGCCG C^GGCCG
Eco52I 3994 CGGCCG C^GGCCG
EcoO65I 2586 GGTCACC G^GT[ACGT]ACC
EcoRI 1248 GAATTC G^AATTC
EcoT22I 2610 ATGCAT ATGCA^T
HhaI 3163 GCGC GCG^C
HinP1I 3163 GCGC G^CGC
KpnI 1703 GGTACC GGTAC^C
MamI 4158 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 725 CAATTG C^AATTG
MluNI 2884 TGGCCA TGG^CCA
MroI 78 TCCGGA T^CCGGA
MscI 2884 TGGCCA TGG^CCA
MunI 725 CAATTG C^AATTG
MvnI 3164 CGCG CG^CG
NsiI 2610 ATGCAT ATGCA^T
NspV 3523 TTCGAA TT^CGAA
PaeR7I 3486 CTCGAG C^TCGAG
PflFI 1393 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 1669 GTTTAAAC GTTT^AAAC
PstI 466 CTGCAG CTGCA^G
PvuII 201 CAGCTG CAG^CTG
RcaI 1203 TCATGA T^CATGA
SacI 2147 GAGCTC GAGCT^C
SapI 2266 GCTCTTC GCTCTTC(1/4)
SfuI 3523 TTCGAA TT^CGAA
SmaI 3628 CCCGGG CCC^GGG
SspBI 399 TGTACA T^GTACA
StuI 3274 AGGCCT AGG^CCT
Tth111I 1393 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2350 TCTAGA T^CTAGA
XcmI 2772 CCACATCAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3486 CTCGAG C^TCGAG
XmaCI 3628 CCCGGG C^CCGGG
XmaI 3628 CCCGGG C^CCGGG
XmnI 4046 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 1630 GGCGTC G[GA]^CG[CT]C
3986 GACGCC
AocI 749 CCTGAGG CC^T[ACGT]AGG
3461 CCTAAGG
BamHI 217 GGATCC G^GATCC
1892 GGATCC
BglI 3276 GCCTCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3427 GCCAGTGGGGC
BsaHI 1630 GGCGTC G[GA]^CG[CT]C
3986 GACGCC
BsaWI 78 TCCGGA [AT]^CCGG[AT]
963 ACCGGA
BsiEI 2174 CGACCG CG[GA][CT]^CG
3994 CGGCCG
BsrBI 2751 CCGCTC CCGCTC(-3/-3)
2582 GAGCGG
BssSI 3870 CACGAG CACGAG(-5/-1)
2997 CTCGTG
BstXI 1683 CCAATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2446 CCAAGAGTTTGG
Bsu36I 749 CCTGAGG CC^T[ACGT]AGG
3461 CCTAAGG
DrdI 2175 GACCGTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3115 GACTGTAGAGTC
DsaI 852 CCATGG C^C[GA][CT]GG
3804 CCATGG
Eco81I 749 CCTGAGG CC^T[ACGT]AGG
3461 CCTAAGG
FauI 3679 CCCGC CCCGC(4/6)
3974 CCCGC
HgaI 3986 GACGC GACGC(5/10)
1631 GCGTC
Hin1I 1630 GGCGTC G[GA]^CG[CT]C
3986 GACGCC
MaeII 1667 ACGT A^CGT
2672 ACGT
MslI 289 CATGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1000 CACTGGTGTG
NcoI 852 CCATGG C^CATGG
3804 CCATGG
PpuMI 3832 AGGACCC [GA]G^G[AT]CC[CT]
3840 GGGACCT
PshAI 3218 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3652 GACTGGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII