##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AgeI        AhdI        AocI        Aor51HI     ApaI        
AscI        AspEI       AviII       BbeI        BciVI       
BclI        BfrI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BsrBI       BsrGI       BssHII      BstAPI      
BstUI       Bsu36I      CfoI        ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoRI       EcoRV       FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HindIII     
HpaI        KasI        KspI        MfeI        MluI        
MroI        MunI        MvnI        NarI        NotI        
NruI        PacI        PaeR7I      PinAI       PmeI        
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SfiI        SfoI        SgrAI       SnaBI       
Sse8387I    SspBI       StuI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I       2407  GGTACC               G^GTACC
AcyI          592  GACGCC               G[GA]^CG[CT]C
AseI          342  ATTAAT               AT^TAAT
AsnI          342  ATTAAT               AT^TAAT
Asp718I      2407  GGTACC               G^GTACC
AspI          322  GACTAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2027  CCCGGG               C^[CT]CG[GA]G
AvrII        2080  CCTAGG               C^CTAGG
BbrPI        2291  CACGTG               CAC^GTG
BbvCI        2677  CCTCAGC              CCTCAGC(-5/-2)
BglII        4218  AGATCT               A^GATCT
BlnI         2080  CCTAGG               C^CTAGG
BpmI         1421  CTCCAG               CTGGAG(16/14)
BsaAI        2291  CACGTG               [CT]AC^GT[GA]
BsaBI        1048  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         592  GACGCC               G[GA]^CG[CT]C
BsoBI        2027  CCCGGG               C^[CT]CG[GA]G
Bst1107I     2530  GTATAC               GTA^TAC
BstBI         569  TTCGAA               TT^CGAA
BstEII       2136  GGTCACC              G^GT[ACGT]ACC
BstPI        2136  GGTCACC              G^GT[ACGT]ACC
BstZ17I      2530  GTATAC               GTA^TAC
DraIII       2369  CACCTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      2136  GGTCACC              G^GT[ACGT]ACC
FauI          500  GCGGG                CCCGC(4/6)
Hin1I         592  GACGCC               G[GA]^CG[CT]C
KpnI         2407  GGTACC               GGTAC^C
MamI         1048  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         1489  GCCGGC               GCC^GGC
NgoMIV       1489  GCCGGC               G^CCGGC
NheI         4063  GCTAGC               G^CTAGC
NspV          569  TTCGAA               TT^CGAA
PflFI         322  GACTAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2291  CACGTG               CAC^GTG
PmlI         2291  CACGTG               CAC^GTG
PshBI         342  ATTAAT               AT^TAAT
PvuII        2830  CAGCTG               CAG^CTG
SexAI        3343  ACCTGGT              A^CC[AT]GGT
SfuI          569  TTCGAA               TT^CGAA
SmaI         2027  CCCGGG               CCC^GGG
SpeI          657  ACTAGT               A^CTAGT
SphI         1373  GCATGC               GCATG^C
SspI         1209  AATATT               AAT^ATT
Tth111I       322  GACTAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          446  TCTAGA               T^CTAGA
XmaCI        2027  CCCGGG               C^CCGGG
XmaI         2027  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI         2530  GTATAC               GT^[AC][GT]AC
             3060  GTAGAC              
AflIII       3997  ACATGT               A^C[GA][CT]GT
             4497  ACATGT              
BalI         1062  TGGCCA               TGG^CCA
             4141  TGGCCA              
BamHI        1999  GGATCC               G^GATCC
             3182  GGATCC              
BcgI          480  CGAGCTCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1746  CGAAGCCTGTGC        
BglI         1790  GCCCCGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3261  GCCGTCTGGGC         
BsgI          644  CTGCAC               GTGCAG(16/14)
             3300  GTGCAG              
BspHI        1303  TCATGA               T^CATGA
             4465  TCATGA              
BspLU11I     3997  ACATGT               A^CATGT
             4497  ACATGT              
BspMI        1454  ACCTGC               ACCTGC(4/8)
             2161  ACCTGC              
BsrDI         533  CATTGC               GCAATG(2/0)
             1728  GCAATG              
BsrFI         935  GCCGGT               [GA]^CCGG[CT]
             1489  GCCGGC              
Cfr10I        935  GCCGGT               [GA]^CCGG[CT]
             1489  GCCGGC              
DraI          258  TTTAAA               TTT^AAA
             2723  TTTAAA              
DrdI          372  GACAGCCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4293  GACAGTGCTGTC        
DsaI           28  CCATGG               C^C[GA][CT]GG
             3187  CCATGG              
EaeI         1062  TGGCCA               [CT]^GGCC[GA]
             4141  TGGCCA              
EcoNI          81  CCTGGAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1516  CCTGTGTCAGG         
HgaI          592  GACGC                GACGC(5/10)
             1725  GACGC               
HincII        381  GTCAAC               GT[CT]^[GA]AC
             4312  GTCAAC              
HindII        381  GTCAAC               GT[CT]^[GA]AC
             4312  GTCAAC              
MluNI        1062  TGGCCA               TGG^CCA
             4141  TGGCCA              
MscI         1062  TGGCCA               TGG^CCA
             4141  TGGCCA              
MspA1I        779  CCGCTG               C[AC]G^C[GT]G
             2830  CAGCTG              
NcoI           28  CCATGG               C^CATGG
             3187  CCATGG              
PpuMI        1909  GGGACCT              [GA]G^G[AT]CC[CT]
             2076  AGGACCT             
PshAI         975  GACATATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2382  GACTGTTGTC          
RcaI         1303  TCATGA               T^CATGA
             4465  TCATGA              
SacI          481  GAGCTC               GAGCT^C
             1386  GAGCTC              
SapI         1355  GAAGAGC              GCTCTTC(1/4)
             3253  GAAGAGC             
ScaI         2513  AGTACT               AGT^ACT
             2787  AGTACT              
XcmI         1824  CCAGCAACAGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2998  CCAATTCTTGAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        Alw44I      
AlwI        AlwNI       ApaLI       ApoI        Asp700I     
AspHI       AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BssSI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CelII       
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRII      EcoT14I     EcoT22I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NdeI        NdeII       NlaIII      NlaIV       
NsiI        NspI        PflMI       PleI        PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       XmnI