##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AspEI AviII BbeI BciVI
BclI BfrI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII BstAPI
BstUI Bsu36I CfoI ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV FbaI FseI
FspI HaeII HhaI HinP1I HindIII
HpaI KasI KspI MfeI MluI
MroI MunI MvnI NarI NotI
NruI PacI PaeR7I PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SnaBI
Sse8387I SspBI StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2407 GGTACC G^GTACC
AcyI 592 GACGCC G[GA]^CG[CT]C
AseI 342 ATTAAT AT^TAAT
AsnI 342 ATTAAT AT^TAAT
Asp718I 2407 GGTACC G^GTACC
AspI 322 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2027 CCCGGG C^[CT]CG[GA]G
AvrII 2080 CCTAGG C^CTAGG
BbrPI 2291 CACGTG CAC^GTG
BbvCI 2677 CCTCAGC CCTCAGC(-5/-2)
BglII 4218 AGATCT A^GATCT
BlnI 2080 CCTAGG C^CTAGG
BpmI 1421 CTCCAG CTGGAG(16/14)
BsaAI 2291 CACGTG [CT]AC^GT[GA]
BsaBI 1048 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 592 GACGCC G[GA]^CG[CT]C
BsoBI 2027 CCCGGG C^[CT]CG[GA]G
Bst1107I 2530 GTATAC GTA^TAC
BstBI 569 TTCGAA TT^CGAA
BstEII 2136 GGTCACC G^GT[ACGT]ACC
BstPI 2136 GGTCACC G^GT[ACGT]ACC
BstZ17I 2530 GTATAC GTA^TAC
DraIII 2369 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2136 GGTCACC G^GT[ACGT]ACC
FauI 500 GCGGG CCCGC(4/6)
Hin1I 592 GACGCC G[GA]^CG[CT]C
KpnI 2407 GGTACC GGTAC^C
MamI 1048 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1489 GCCGGC GCC^GGC
NgoMIV 1489 GCCGGC G^CCGGC
NheI 4063 GCTAGC G^CTAGC
NspV 569 TTCGAA TT^CGAA
PflFI 322 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2291 CACGTG CAC^GTG
PmlI 2291 CACGTG CAC^GTG
PshBI 342 ATTAAT AT^TAAT
PvuII 2830 CAGCTG CAG^CTG
SexAI 3343 ACCTGGT A^CC[AT]GGT
SfuI 569 TTCGAA TT^CGAA
SmaI 2027 CCCGGG CCC^GGG
SpeI 657 ACTAGT A^CTAGT
SphI 1373 GCATGC GCATG^C
SspI 1209 AATATT AAT^ATT
Tth111I 322 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 446 TCTAGA T^CTAGA
XmaCI 2027 CCCGGG C^CCGGG
XmaI 2027 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2530 GTATAC GT^[AC][GT]AC
3060 GTAGAC
AflIII 3997 ACATGT A^C[GA][CT]GT
4497 ACATGT
BalI 1062 TGGCCA TGG^CCA
4141 TGGCCA
BamHI 1999 GGATCC G^GATCC
3182 GGATCC
BcgI 480 CGAGCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1746 CGAAGCCTGTGC
BglI 1790 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3261 GCCGTCTGGGC
BsgI 644 CTGCAC GTGCAG(16/14)
3300 GTGCAG
BspHI 1303 TCATGA T^CATGA
4465 TCATGA
BspLU11I 3997 ACATGT A^CATGT
4497 ACATGT
BspMI 1454 ACCTGC ACCTGC(4/8)
2161 ACCTGC
BsrDI 533 CATTGC GCAATG(2/0)
1728 GCAATG
BsrFI 935 GCCGGT [GA]^CCGG[CT]
1489 GCCGGC
Cfr10I 935 GCCGGT [GA]^CCGG[CT]
1489 GCCGGC
DraI 258 TTTAAA TTT^AAA
2723 TTTAAA
DrdI 372 GACAGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4293 GACAGTGCTGTC
DsaI 28 CCATGG C^C[GA][CT]GG
3187 CCATGG
EaeI 1062 TGGCCA [CT]^GGCC[GA]
4141 TGGCCA
EcoNI 81 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1516 CCTGTGTCAGG
HgaI 592 GACGC GACGC(5/10)
1725 GACGC
HincII 381 GTCAAC GT[CT]^[GA]AC
4312 GTCAAC
HindII 381 GTCAAC GT[CT]^[GA]AC
4312 GTCAAC
MluNI 1062 TGGCCA TGG^CCA
4141 TGGCCA
MscI 1062 TGGCCA TGG^CCA
4141 TGGCCA
MspA1I 779 CCGCTG C[AC]G^C[GT]G
2830 CAGCTG
NcoI 28 CCATGG C^CATGG
3187 CCATGG
PpuMI 1909 GGGACCT [GA]G^G[AT]CC[CT]
2076 AGGACCT
PshAI 975 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2382 GACTGTTGTC
RcaI 1303 TCATGA T^CATGA
4465 TCATGA
SacI 481 GAGCTC GAGCT^C
1386 GAGCTC
SapI 1355 GAAGAGC GCTCTTC(1/4)
3253 GAAGAGC
ScaI 2513 AGTACT AGT^ACT
2787 AGTACT
XcmI 1824 CCAGCAACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2998 CCAATTCTTGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BssSI BstF5I
BstNI BstXI BstYI Cac8I CelII
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI