################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII AccII AccIII AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AspEI AviII BbeI BciVI BclI BfrI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII BstAPI BstUI Bsu36I CfoI ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FbaI FseI FspI HaeII HhaI HinP1I HindIII HpaI KasI KspI MfeI MluI MroI MunI MvnI NarI NotI NruI PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SnaBI Sse8387I SspBI StuI SwaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 2407 GGTACC G^GTACC AcyI 592 GACGCC G[GA]^CG[CT]C AseI 342 ATTAAT AT^TAAT AsnI 342 ATTAAT AT^TAAT Asp718I 2407 GGTACC G^GTACC AspI 322 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2027 CCCGGG C^[CT]CG[GA]G AvrII 2080 CCTAGG C^CTAGG BbrPI 2291 CACGTG CAC^GTG BbvCI 2677 CCTCAGC CCTCAGC(-5/-2) BglII 4218 AGATCT A^GATCT BlnI 2080 CCTAGG C^CTAGG BpmI 1421 CTCCAG CTGGAG(16/14) BsaAI 2291 CACGTG [CT]AC^GT[GA] BsaBI 1048 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 592 GACGCC G[GA]^CG[CT]C BsoBI 2027 CCCGGG C^[CT]CG[GA]G Bst1107I 2530 GTATAC GTA^TAC BstBI 569 TTCGAA TT^CGAA BstEII 2136 GGTCACC G^GT[ACGT]ACC BstPI 2136 GGTCACC G^GT[ACGT]ACC BstZ17I 2530 GTATAC GTA^TAC DraIII 2369 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 2136 GGTCACC G^GT[ACGT]ACC FauI 500 GCGGG CCCGC(4/6) Hin1I 592 GACGCC G[GA]^CG[CT]C KpnI 2407 GGTACC GGTAC^C MamI 1048 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 1489 GCCGGC GCC^GGC NgoMIV 1489 GCCGGC G^CCGGC NheI 4063 GCTAGC G^CTAGC NspV 569 TTCGAA TT^CGAA PflFI 322 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2291 CACGTG CAC^GTG PmlI 2291 CACGTG CAC^GTG PshBI 342 ATTAAT AT^TAAT PvuII 2830 CAGCTG CAG^CTG SexAI 3343 ACCTGGT A^CC[AT]GGT SfuI 569 TTCGAA TT^CGAA SmaI 2027 CCCGGG CCC^GGG SpeI 657 ACTAGT A^CTAGT SphI 1373 GCATGC GCATG^C SspI 1209 AATATT AAT^ATT Tth111I 322 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 446 TCTAGA T^CTAGA XmaCI 2027 CCCGGG C^CCGGG XmaI 2027 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 2530 GTATAC GT^[AC][GT]AC 3060 GTAGAC AflIII 3997 ACATGT A^C[GA][CT]GT 4497 ACATGT BalI 1062 TGGCCA TGG^CCA 4141 TGGCCA BamHI 1999 GGATCC G^GATCC 3182 GGATCC BcgI 480 CGAGCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1746 CGAAGCCTGTGC BglI 1790 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3261 GCCGTCTGGGC BsgI 644 CTGCAC GTGCAG(16/14) 3300 GTGCAG BspHI 1303 TCATGA T^CATGA 4465 TCATGA BspLU11I 3997 ACATGT A^CATGT 4497 ACATGT BspMI 1454 ACCTGC ACCTGC(4/8) 2161 ACCTGC BsrDI 533 CATTGC GCAATG(2/0) 1728 GCAATG BsrFI 935 GCCGGT [GA]^CCGG[CT] 1489 GCCGGC Cfr10I 935 GCCGGT [GA]^CCGG[CT] 1489 GCCGGC DraI 258 TTTAAA TTT^AAA 2723 TTTAAA DrdI 372 GACAGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4293 GACAGTGCTGTC DsaI 28 CCATGG C^C[GA][CT]GG 3187 CCATGG EaeI 1062 TGGCCA [CT]^GGCC[GA] 4141 TGGCCA EcoNI 81 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1516 CCTGTGTCAGG HgaI 592 GACGC GACGC(5/10) 1725 GACGC HincII 381 GTCAAC GT[CT]^[GA]AC 4312 GTCAAC HindII 381 GTCAAC GT[CT]^[GA]AC 4312 GTCAAC MluNI 1062 TGGCCA TGG^CCA 4141 TGGCCA MscI 1062 TGGCCA TGG^CCA 4141 TGGCCA MspA1I 779 CCGCTG C[AC]G^C[GT]G 2830 CAGCTG NcoI 28 CCATGG C^CATGG 3187 CCATGG PpuMI 1909 GGGACCT [GA]G^G[AT]CC[CT] 2076 AGGACCT PshAI 975 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2382 GACTGTTGTC RcaI 1303 TCATGA T^CATGA 4465 TCATGA SacI 481 GAGCTC GAGCT^C 1386 GAGCTC SapI 1355 GAAGAGC GCTCTTC(1/4) 3253 GAAGAGC ScaI 2513 AGTACT AGT^ACT 2787 AGTACT XcmI 1824 CCAGCAACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2998 CCAATTCTTGAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI Bpu1102I BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Cac8I CelII Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI