##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AclI AflII AgeI Alw44I ApaLI
AscI Asp700I AspI AviII BamHI
BbeI BbrPI BfrI BglII BsiEI
BsiWI BspDI BsrBI BssHII BssSI
BstBI ClaI CpoI DraIII DrdI
EagI EclXI Eco52I EcoT22I FseI
FspI HpaI KasI KspI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI PvuII
RsrII SacII SalI SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 49 GGTACC G^GTACC
AccIII 3241 TCCGGA T^CCGGA
AhdI 4411 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 629 CCTGAGG CC^T[ACGT]AGG
Aor51HI 939 AGCGCT AGC^GCT
ApaI 2505 GGGCCC GGGCC^C
AseI 4552 ATTAAT AT^TAAT
AsnI 4552 ATTAAT AT^TAAT
Asp718I 49 GGTACC G^GTACC
AspEI 4411 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 1602 CCTCAGC CCTCAGC(-5/-2)
BcgI 572 CGAAATGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2507 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 300 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 300 GCTTAGC GC^T[ACGT]AGC
BsaAI 3794 TACGTG [CT]AC^GT[GA]
BsaBI 830 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3241 TCCGGA T^CCGGA
Bsp1407I 4518 TGTACA T^GTACA
BspEI 3241 TCCGGA T^CCGGA
BsrGI 4518 TGTACA T^GTACA
Bst1107I 4464 GTATAC GTA^TAC
BstAPI 1858 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3608 GGTTACC G^GT[ACGT]ACC
BstPI 3608 GGTTACC G^GT[ACGT]ACC
BstZ17I 4464 GTATAC GTA^TAC
Bsu36I 629 CCTGAGG CC^T[ACGT]AGG
CelII 300 GCTTAGC GC^T[ACGT]AGC
Eam1105I 4411 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 939 AGCGCT AGC^GCT
Eco81I 629 CCTGAGG CC^T[ACGT]AGG
EcoNI 806 CCTTTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3608 GGTTACC G^GT[ACGT]ACC
EcoRI 2773 GAATTC G^AATTC
EcoRV 2195 GATATC GAT^ATC
HgaI 61 GACGC GACGC(5/10)
KpnI 49 GGTACC GGTAC^C
MamI 830 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3498 CAATTG C^AATTG
MroI 3241 TCCGGA T^CCGGA
MunI 3498 CAATTG C^AATTG
PshAI 4193 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4552 ATTAAT AT^TAAT
SapI 2096 GAAGAGC GCTCTTC(1/4)
SfiI 2506 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 4383 CTCAAG C^T[CT][GA]AG
SspBI 4518 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 3586 GACGTC GACGT^C
4784 GACGTC
AccII 1492 CGCG CG^CG
2052 CGCG
AcyI 3586 GACGTC G[GA]^CG[CT]C
4784 GACGTC
AvrII 3025 CCTAGG C^CTAGG
4359 CCTAGG
BalI 259 TGGCCA TGG^CCA
4499 TGGCCA
BclI 3507 TGATCA T^GATCA
3567 TGATCA
BlnI 3025 CCTAGG C^CTAGG
4359 CCTAGG
BsaHI 3586 GACGTC G[GA]^CG[CT]C
4784 GACGTC
BsaWI 2937 TCCGGT [AT]^CCGG[AT]
3241 TCCGGA
BsmBI 59 GAGACG CGTCTC(1/5)
2450 GAGACG
BsmFI 1837 GTCCC GGGAC(10/14)
2899 GTCCC
BspLU11I 4323 ACATGT A^CATGT
4489 ACATGT
BspMI 1254 ACCTGC ACCTGC(4/8)
2872 GCAGGT
BstUI 1492 CGCG CG^CG
2052 CGCG
DsaI 987 CCATGG C^C[GA][CT]GG
3288 CCATGG
FbaI 3507 TGATCA T^GATCA
3567 TGATCA
HaeII 939 AGCGCT [GA]GCGC^[CT]
3754 GGCGCT
Hin1I 3586 GACGTC G[GA]^CG[CT]C
4784 GACGTC
HincII 1309 GTCAAC GT[CT]^[GA]AC
2876 GTTGAC
HindII 1309 GTCAAC GT[CT]^[GA]AC
2876 GTTGAC
HindIII 1450 AAGCTT A^AGCTT
4031 AAGCTT
MluI 1491 ACGCGT A^CGCGT
2051 ACGCGT
MluNI 259 TGGCCA TGG^CCA
4499 TGGCCA
MscI 259 TGGCCA TGG^CCA
4499 TGGCCA
MslI 1979 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3083 CACTATTATG
MspA1I 3354 CCGCTG C[AC]G^C[GT]G
4099 CAGCGG
MvnI 1492 CGCG CG^CG
2052 CGCG
NcoI 987 CCATGG C^CATGG
3288 CCATGG
PpuMI 2481 AGGTCCT [GA]G^G[AT]CC[CT]
3307 AGGACCC
SexAI 972 ACCTGGT A^CC[AT]GGT
4125 ACCAGGT
SmaI 28 CCCGGG CCC^GGG
36 CCCGGG
SphI 1654 GCATGC GCATG^C
3205 GCATGC
XmaCI 28 CCCGGG C^CCGGG
36 CCCGGG
XmaI 28 CCCGGG C^CCGGG
36 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmI BsoBI Bsp1286I BspHI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NaeI NciI
NdeI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PstI RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII