################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AclI AflII AgeI Alw44I ApaLI AscI Asp700I AspI AviII BamHI BbeI BbrPI BfrI BglII BsiEI BsiWI BspDI BsrBI BssHII BssSI BstBI ClaI CpoI DraIII DrdI EagI EclXI Eco52I EcoT22I FseI FspI HpaI KasI KspI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 49 GGTACC G^GTACC AccIII 3241 TCCGGA T^CCGGA AhdI 4411 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 629 CCTGAGG CC^T[ACGT]AGG Aor51HI 939 AGCGCT AGC^GCT ApaI 2505 GGGCCC GGGCC^C AseI 4552 ATTAAT AT^TAAT AsnI 4552 ATTAAT AT^TAAT Asp718I 49 GGTACC G^GTACC AspEI 4411 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbvCI 1602 CCTCAGC CCTCAGC(-5/-2) BcgI 572 CGAAATGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 2507 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 300 GCTTAGC GC^T[ACGT]AGC Bpu1102I 300 GCTTAGC GC^T[ACGT]AGC BsaAI 3794 TACGTG [CT]AC^GT[GA] BsaBI 830 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3241 TCCGGA T^CCGGA Bsp1407I 4518 TGTACA T^GTACA BspEI 3241 TCCGGA T^CCGGA BsrGI 4518 TGTACA T^GTACA Bst1107I 4464 GTATAC GTA^TAC BstAPI 1858 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3608 GGTTACC G^GT[ACGT]ACC BstPI 3608 GGTTACC G^GT[ACGT]ACC BstZ17I 4464 GTATAC GTA^TAC Bsu36I 629 CCTGAGG CC^T[ACGT]AGG CelII 300 GCTTAGC GC^T[ACGT]AGC Eam1105I 4411 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 939 AGCGCT AGC^GCT Eco81I 629 CCTGAGG CC^T[ACGT]AGG EcoNI 806 CCTTTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3608 GGTTACC G^GT[ACGT]ACC EcoRI 2773 GAATTC G^AATTC EcoRV 2195 GATATC GAT^ATC HgaI 61 GACGC GACGC(5/10) KpnI 49 GGTACC GGTAC^C MamI 830 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3498 CAATTG C^AATTG MroI 3241 TCCGGA T^CCGGA MunI 3498 CAATTG C^AATTG PshAI 4193 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4552 ATTAAT AT^TAAT SapI 2096 GAAGAGC GCTCTTC(1/4) SfiI 2506 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 4383 CTCAAG C^T[CT][GA]AG SspBI 4518 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AatII 3586 GACGTC GACGT^C 4784 GACGTC AccII 1492 CGCG CG^CG 2052 CGCG AcyI 3586 GACGTC G[GA]^CG[CT]C 4784 GACGTC AvrII 3025 CCTAGG C^CTAGG 4359 CCTAGG BalI 259 TGGCCA TGG^CCA 4499 TGGCCA BclI 3507 TGATCA T^GATCA 3567 TGATCA BlnI 3025 CCTAGG C^CTAGG 4359 CCTAGG BsaHI 3586 GACGTC G[GA]^CG[CT]C 4784 GACGTC BsaWI 2937 TCCGGT [AT]^CCGG[AT] 3241 TCCGGA BsmBI 59 GAGACG CGTCTC(1/5) 2450 GAGACG BsmFI 1837 GTCCC GGGAC(10/14) 2899 GTCCC BspLU11I 4323 ACATGT A^CATGT 4489 ACATGT BspMI 1254 ACCTGC ACCTGC(4/8) 2872 GCAGGT BstUI 1492 CGCG CG^CG 2052 CGCG DsaI 987 CCATGG C^C[GA][CT]GG 3288 CCATGG FbaI 3507 TGATCA T^GATCA 3567 TGATCA HaeII 939 AGCGCT [GA]GCGC^[CT] 3754 GGCGCT Hin1I 3586 GACGTC G[GA]^CG[CT]C 4784 GACGTC HincII 1309 GTCAAC GT[CT]^[GA]AC 2876 GTTGAC HindII 1309 GTCAAC GT[CT]^[GA]AC 2876 GTTGAC HindIII 1450 AAGCTT A^AGCTT 4031 AAGCTT MluI 1491 ACGCGT A^CGCGT 2051 ACGCGT MluNI 259 TGGCCA TGG^CCA 4499 TGGCCA MscI 259 TGGCCA TGG^CCA 4499 TGGCCA MslI 1979 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3083 CACTATTATG MspA1I 3354 CCGCTG C[AC]G^C[GT]G 4099 CAGCGG MvnI 1492 CGCG CG^CG 2052 CGCG NcoI 987 CCATGG C^CATGG 3288 CCATGG PpuMI 2481 AGGTCCT [GA]G^G[AT]CC[CT] 3307 AGGACCC SexAI 972 ACCTGGT A^CC[AT]GGT 4125 ACCAGGT SmaI 28 CCCGGG CCC^GGG 36 CCCGGG SphI 1654 GCATGC GCATG^C 3205 GCATGC XmaCI 28 CCCGGG C^CCGGG 36 CCCGGG XmaI 28 CCCGGG C^CCGGG 36 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmI BsoBI Bsp1286I BspHI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NaeI NciI NdeI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII