##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AclI        AflII       AgeI        Alw44I      ApaLI       
AscI        Asp700I     AspI        AviII       BamHI       
BbeI        BbrPI       BfrI        BglII       BsiEI       
BsiWI       BspDI       BsrBI       BssHII      BssSI       
BstBI       ClaI        CpoI        DraIII      DrdI        
EagI        EclXI       Eco52I      EcoT22I     FseI        
FspI        HpaI        KasI        KspI        NarI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        PvuII       
RsrII       SacII       SalI        SbfI        ScaI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspI        SwaI        Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I         49  GGTACC               G^GTACC
AccIII       3241  TCCGGA               T^CCGGA
AhdI         4411  GACTTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          629  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       939  AGCGCT               AGC^GCT
ApaI         2505  GGGCCC               GGGCC^C
AseI         4552  ATTAAT               AT^TAAT
AsnI         4552  ATTAAT               AT^TAAT
Asp718I        49  GGTACC               G^GTACC
AspEI        4411  GACTTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI        1602  CCTCAGC              CCTCAGC(-5/-2)
BcgI          572  CGAAATGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         2507  GCCCTGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          300  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      300  GCTTAGC              GC^T[ACGT]AGC
BsaAI        3794  TACGTG               [CT]AC^GT[GA]
BsaBI         830  GATAAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        3241  TCCGGA               T^CCGGA
Bsp1407I     4518  TGTACA               T^GTACA
BspEI        3241  TCCGGA               T^CCGGA
BsrGI        4518  TGTACA               T^GTACA
Bst1107I     4464  GTATAC               GTA^TAC
BstAPI       1858  GCACACACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3608  GGTTACC              G^GT[ACGT]ACC
BstPI        3608  GGTTACC              G^GT[ACGT]ACC
BstZ17I      4464  GTATAC               GTA^TAC
Bsu36I        629  CCTGAGG              CC^T[ACGT]AGG
CelII         300  GCTTAGC              GC^T[ACGT]AGC
Eam1105I     4411  GACTTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      939  AGCGCT               AGC^GCT
Eco81I        629  CCTGAGG              CC^T[ACGT]AGG
EcoNI         806  CCTTTGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3608  GGTTACC              G^GT[ACGT]ACC
EcoRI        2773  GAATTC               G^AATTC
EcoRV        2195  GATATC               GAT^ATC
HgaI           61  GACGC                GACGC(5/10)
KpnI           49  GGTACC               GGTAC^C
MamI          830  GATAAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3498  CAATTG               C^AATTG
MroI         3241  TCCGGA               T^CCGGA
MunI         3498  CAATTG               C^AATTG
PshAI        4193  GACTCACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4552  ATTAAT               AT^TAAT
SapI         2096  GAAGAGC              GCTCTTC(1/4)
SfiI         2506  GGCCCTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI         4383  CTCAAG               C^T[CT][GA]AG
SspBI        4518  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AatII        3586  GACGTC               GACGT^C
             4784  GACGTC              
AccII        1492  CGCG                 CG^CG
             2052  CGCG                
AcyI         3586  GACGTC               G[GA]^CG[CT]C
             4784  GACGTC              
AvrII        3025  CCTAGG               C^CTAGG
             4359  CCTAGG              
BalI          259  TGGCCA               TGG^CCA
             4499  TGGCCA              
BclI         3507  TGATCA               T^GATCA
             3567  TGATCA              
BlnI         3025  CCTAGG               C^CTAGG
             4359  CCTAGG              
BsaHI        3586  GACGTC               G[GA]^CG[CT]C
             4784  GACGTC              
BsaWI        2937  TCCGGT               [AT]^CCGG[AT]
             3241  TCCGGA              
BsmBI          59  GAGACG               CGTCTC(1/5)
             2450  GAGACG              
BsmFI        1837  GTCCC                GGGAC(10/14)
             2899  GTCCC               
BspLU11I     4323  ACATGT               A^CATGT
             4489  ACATGT              
BspMI        1254  ACCTGC               ACCTGC(4/8)
             2872  GCAGGT              
BstUI        1492  CGCG                 CG^CG
             2052  CGCG                
DsaI          987  CCATGG               C^C[GA][CT]GG
             3288  CCATGG              
FbaI         3507  TGATCA               T^GATCA
             3567  TGATCA              
HaeII         939  AGCGCT               [GA]GCGC^[CT]
             3754  GGCGCT              
Hin1I        3586  GACGTC               G[GA]^CG[CT]C
             4784  GACGTC              
HincII       1309  GTCAAC               GT[CT]^[GA]AC
             2876  GTTGAC              
HindII       1309  GTCAAC               GT[CT]^[GA]AC
             2876  GTTGAC              
HindIII      1450  AAGCTT               A^AGCTT
             4031  AAGCTT              
MluI         1491  ACGCGT               A^CGCGT
             2051  ACGCGT              
MluNI         259  TGGCCA               TGG^CCA
             4499  TGGCCA              
MscI          259  TGGCCA               TGG^CCA
             4499  TGGCCA              
MslI         1979  CATCTGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3083  CACTATTATG          
MspA1I       3354  CCGCTG               C[AC]G^C[GT]G
             4099  CAGCGG              
MvnI         1492  CGCG                 CG^CG
             2052  CGCG                
NcoI          987  CCATGG               C^CATGG
             3288  CCATGG              
PpuMI        2481  AGGTCCT              [GA]G^G[AT]CC[CT]
             3307  AGGACCC             
SexAI         972  ACCTGGT              A^CC[AT]GGT
             4125  ACCAGGT             
SmaI           28  CCCGGG               CCC^GGG
               36  CCCGGG              
SphI         1654  GCATGC               GCATG^C
             3205  GCATGC              
XmaCI          28  CCCGGG               C^CCGGG
               36  CCCGGG              
XmaI           28  CCCGGG               C^CCGGG
               36  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvI        BciVI       BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmI        BsoBI       Bsp1286I    BspHI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspI        MvaI        MwoI        NaeI        NciI        
NdeI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PstI        RcaI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII