##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AflII AgeI Aor51HI
AscI Asp718I AviII AvrII BamHI
BbrPI BcgI BciVI BfrI BglII
BlnI BsiWI BspDI BspHI BstBI
BstEII BstPI ClaI DrdI Eco47III
EcoNI EcoO65I EcoT22I FseI FspI
KpnI MluI NdeI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PvuI PvuII
RcaI SalI SbfI SfuI SgrAI
SnaBI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccIII 67 TCCGGA T^CCGGA
AclI 846 AACGTT AA^CGTT
AhdI 4064 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2243 GTGCAC G^TGCAC
ApaLI 2243 GTGCAC G^TGCAC
AseI 4915 ATTAAT AT^TAAT
AsnI 4915 ATTAAT AT^TAAT
Asp700I 3097 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4064 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 865 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3687 TGGCCA TGG^CCA
BbeI 100 GGCGCC GGCGC^C
BclI 1248 TGATCA T^GATCA
BlpI 736 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 736 GCTGAGC GC^T[ACGT]AGC
BsaAI 3398 TACGTG [CT]AC^GT[GA]
BsaBI 1243 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 67 TCCGGA T^CCGGA
BsmI 1949 GCATTC GAATGC(1/-1)
BspEI 67 TCCGGA T^CCGGA
BsrDI 4865 CATTGC GCAATG(2/0)
BssHII 298 GCGCGC G^CGCGC
CelII 736 GCTGAGC GC^T[ACGT]AGC
CpoI 350 CGGACCG CG^G[AT]CCG
Eam1105I 4064 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 814 GATATC GAT^ATC
FbaI 1248 TGATCA T^GATCA
HindIII 1108 AAGCTT A^AGCTT
KasI 100 GGCGCC G^GCGCC
KspI 312 CCGCGG CCGC^GG
MamI 1243 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2471 CAATTG C^AATTG
MluNI 3687 TGGCCA TGG^CCA
MroI 67 TCCGGA T^CCGGA
MscI 3687 TGGCCA TGG^CCA
MunI 2471 CAATTG C^AATTG
NaeI 376 GCCGGC GCC^GGC
NarI 100 GGCGCC GG^CGCC
NgoMIV 376 GCCGGC G^CCGGC
NheI 4334 GCTAGC G^CTAGC
PaeR7I 202 CTCGAG C^TCGAG
PflFI 865 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2470 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1269 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 4915 ATTAAT AT^TAAT
Psp1406I 846 AACGTT AA^CGTT
RsrII 350 CGGACCG CG^G[AT]CCG
SacII 312 CCGCGG CCGC^GG
SapI 2866 GAAGAGC GCTCTTC(1/4)
SexAI 866 ACCAGGT A^CC[AT]GGT
SfiI 549 GGCCGGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 100 GGCGCC GGC^GCC
SmaI 3008 CCCGGG CCC^GGG
SpeI 2247 ACTAGT A^CTAGT
SspI 4908 AATATT AAT^ATT
StuI 3693 AGGCCT AGG^CCT
Tth111I 865 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2470 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4462 TCTAGA T^CTAGA
XhoI 202 CTCGAG C^TCGAG
XmaCI 3008 CCCGGG C^CCGGG
XmaI 3008 CCCGGG C^CCGGG
XmnI 3097 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 2802 GTATAC GT^[AC][GT]AC
4206 GTATAC
AcyI 55 GACGCC G[GA]^CG[CT]C
100 GGCGCC
AflIII 2306 ACATGT A^C[GA][CT]GT
4844 ACATGT
AlwI 1286 GATCC GGATC(4/5)
4015 GATCC
AocI 2729 CCTTAGG CC^T[ACGT]AGG
4570 CCTTAGG
BanI 100 GGCGCC G^G[CT][GA]CC
210 GGTGCC
BbvCI 2673 CCTCAGC CCTCAGC(-5/-2)
4419 GCTGAGG
BsaHI 55 GACGCC G[GA]^CG[CT]C
100 GGCGCC
BsaWI 32 TCCGGT [AT]^CCGG[AT]
67 TCCGGA
BsiEI 533 CGGCCG CG[GA][CT]^CG
548 CGGCCG
BsmBI 53 GAGACG CGTCTC(1/5)
126 GAGACG
Bsp1407I 2030 TGTACA T^GTACA
2573 TGTACA
BspLU11I 2306 ACATGT A^CATGT
4844 ACATGT
BspMI 563 GCAGGT ACCTGC(4/8)
887 GCAGGT
BsrFI 207 GCCGGT [GA]^CCGG[CT]
376 GCCGGC
BsrGI 2030 TGTACA T^GTACA
2573 TGTACA
BssSI 767 CACGAG CACGAG(-5/-1)
2216 CACGAG
Bst1107I 2802 GTATAC GTA^TAC
4206 GTATAC
BstXI 1213 CCAGTGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1781 CCATGGATATGG
BstYI 1285 AGATCC [GA]^GATC[CT]
4014 AGATCC
BstZ17I 2802 GTATAC GTA^TAC
4206 GTATAC
Bsu36I 2729 CCTTAGG CC^T[ACGT]AGG
4570 CCTTAGG
Cfr10I 207 GCCGGT [GA]^CCGG[CT]
376 GCCGGC
DraIII 1598 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4297 CACATAGTG
EagI 533 CGGCCG C^GGCCG
548 CGGCCG
EclXI 533 CGGCCG C^GGCCG
548 CGGCCG
Eco52I 533 CGGCCG C^GGCCG
548 CGGCCG
Eco81I 2729 CCTTAGG CC^T[ACGT]AGG
4570 CCTTAGG
EcoRI 784 GAATTC G^AATTC
2131 GAATTC
HaeII 100 GGCGCC [GA]GCGC^[CT]
414 GGCGCT
HgaI 55 GACGC GACGC(5/10)
128 GACGC
Hin1I 55 GACGCC G[GA]^CG[CT]C
100 GGCGCC
HpaI 4116 GTTAAC GTT^AAC
4195 GTTAAC
MflI 1285 AGATCC [GA]^GATC[CT]
4014 AGATCC
NcoI 1781 CCATGG C^CATGG
4897 CCATGG
ScaI 1317 AGTACT AGT^ACT
1441 AGTACT
SmlI 202 CTCGAG C^T[CT][GA]AG
1089 CTTGAG
SspBI 2030 TGTACA T^GTACA
2573 TGTACA
XcmI 1781 CCATGGATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2960 CCAAAAGCATGATGG
XhoII 1285 AGATCC [GA]^GATC[CT]
4014 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI ApaI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I