##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

AatII       Acc65I      AflII       AgeI        Aor51HI     
AscI        Asp718I     AviII       AvrII       BamHI       
BbrPI       BcgI        BciVI       BfrI        BglII       
BlnI        BsiWI       BspDI       BspHI       BstBI       
BstEII      BstPI       ClaI        DrdI        Eco47III    
EcoNI       EcoO65I     EcoT22I     FseI        FspI        
KpnI        MluI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       PvuI        PvuII       
RcaI        SalI        SbfI        SfuI        SgrAI       
SnaBI       SphI        Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccIII         67  TCCGGA               T^CCGGA
AclI          846  AACGTT               AA^CGTT
AhdI         4064  GACCAGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2243  GTGCAC               G^TGCAC
ApaLI        2243  GTGCAC               G^TGCAC
AseI         4915  ATTAAT               AT^TAAT
AsnI         4915  ATTAAT               AT^TAAT
Asp700I      3097  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        4064  GACCAGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          865  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         3687  TGGCCA               TGG^CCA
BbeI          100  GGCGCC               GGCGC^C
BclI         1248  TGATCA               T^GATCA
BlpI          736  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      736  GCTGAGC              GC^T[ACGT]AGC
BsaAI        3398  TACGTG               [CT]AC^GT[GA]
BsaBI        1243  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI          67  TCCGGA               T^CCGGA
BsmI         1949  GCATTC               GAATGC(1/-1)
BspEI          67  TCCGGA               T^CCGGA
BsrDI        4865  CATTGC               GCAATG(2/0)
BssHII        298  GCGCGC               G^CGCGC
CelII         736  GCTGAGC              GC^T[ACGT]AGC
CpoI          350  CGGACCG              CG^G[AT]CCG
Eam1105I     4064  GACCAGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV         814  GATATC               GAT^ATC
FbaI         1248  TGATCA               T^GATCA
HindIII      1108  AAGCTT               A^AGCTT
KasI          100  GGCGCC               G^GCGCC
KspI          312  CCGCGG               CCGC^GG
MamI         1243  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2471  CAATTG               C^AATTG
MluNI        3687  TGGCCA               TGG^CCA
MroI           67  TCCGGA               T^CCGGA
MscI         3687  TGGCCA               TGG^CCA
MunI         2471  CAATTG               C^AATTG
NaeI          376  GCCGGC               GCC^GGC
NarI          100  GGCGCC               GG^CGCC
NgoMIV        376  GCCGGC               G^CCGGC
NheI         4334  GCTAGC               G^CTAGC
PaeR7I        202  CTCGAG               C^TCGAG
PflFI         865  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2470  CCAATTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1269  GGGTCCC              [GA]G^G[AT]CC[CT]
PshBI        4915  ATTAAT               AT^TAAT
Psp1406I      846  AACGTT               AA^CGTT
RsrII         350  CGGACCG              CG^G[AT]CCG
SacII         312  CCGCGG               CCGC^GG
SapI         2866  GAAGAGC              GCTCTTC(1/4)
SexAI         866  ACCAGGT              A^CC[AT]GGT
SfiI          549  GGCCGGAGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          100  GGCGCC               GGC^GCC
SmaI         3008  CCCGGG               CCC^GGG
SpeI         2247  ACTAGT               A^CTAGT
SspI         4908  AATATT               AAT^ATT
StuI         3693  AGGCCT               AGG^CCT
Tth111I       865  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2470  CCAATTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         4462  TCTAGA               T^CTAGA
XhoI          202  CTCGAG               C^TCGAG
XmaCI        3008  CCCGGG               C^CCGGG
XmaI         3008  CCCGGG               C^CCGGG
XmnI         3097  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AccI         2802  GTATAC               GT^[AC][GT]AC
             4206  GTATAC              
AcyI           55  GACGCC               G[GA]^CG[CT]C
              100  GGCGCC              
AflIII       2306  ACATGT               A^C[GA][CT]GT
             4844  ACATGT              
AlwI         1286  GATCC                GGATC(4/5)
             4015  GATCC               
AocI         2729  CCTTAGG              CC^T[ACGT]AGG
             4570  CCTTAGG             
BanI          100  GGCGCC               G^G[CT][GA]CC
              210  GGTGCC              
BbvCI        2673  CCTCAGC              CCTCAGC(-5/-2)
             4419  GCTGAGG             
BsaHI          55  GACGCC               G[GA]^CG[CT]C
              100  GGCGCC              
BsaWI          32  TCCGGT               [AT]^CCGG[AT]
               67  TCCGGA              
BsiEI         533  CGGCCG               CG[GA][CT]^CG
              548  CGGCCG              
BsmBI          53  GAGACG               CGTCTC(1/5)
              126  GAGACG              
Bsp1407I     2030  TGTACA               T^GTACA
             2573  TGTACA              
BspLU11I     2306  ACATGT               A^CATGT
             4844  ACATGT              
BspMI         563  GCAGGT               ACCTGC(4/8)
              887  GCAGGT              
BsrFI         207  GCCGGT               [GA]^CCGG[CT]
              376  GCCGGC              
BsrGI        2030  TGTACA               T^GTACA
             2573  TGTACA              
BssSI         767  CACGAG               CACGAG(-5/-1)
             2216  CACGAG              
Bst1107I     2802  GTATAC               GTA^TAC
             4206  GTATAC              
BstXI        1213  CCAGTGATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1781  CCATGGATATGG        
BstYI        1285  AGATCC               [GA]^GATC[CT]
             4014  AGATCC              
BstZ17I      2802  GTATAC               GTA^TAC
             4206  GTATAC              
Bsu36I       2729  CCTTAGG              CC^T[ACGT]AGG
             4570  CCTTAGG             
Cfr10I        207  GCCGGT               [GA]^CCGG[CT]
              376  GCCGGC              
DraIII       1598  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4297  CACATAGTG           
EagI          533  CGGCCG               C^GGCCG
              548  CGGCCG              
EclXI         533  CGGCCG               C^GGCCG
              548  CGGCCG              
Eco52I        533  CGGCCG               C^GGCCG
              548  CGGCCG              
Eco81I       2729  CCTTAGG              CC^T[ACGT]AGG
             4570  CCTTAGG             
EcoRI         784  GAATTC               G^AATTC
             2131  GAATTC              
HaeII         100  GGCGCC               [GA]GCGC^[CT]
              414  GGCGCT              
HgaI           55  GACGC                GACGC(5/10)
              128  GACGC               
Hin1I          55  GACGCC               G[GA]^CG[CT]C
              100  GGCGCC              
HpaI         4116  GTTAAC               GTT^AAC
             4195  GTTAAC              
MflI         1285  AGATCC               [GA]^GATC[CT]
             4014  AGATCC              
NcoI         1781  CCATGG               C^CATGG
             4897  CCATGG              
ScaI         1317  AGTACT               AGT^ACT
             1441  AGTACT              
SmlI          202  CTCGAG               C^T[CT][GA]AG
             1089  CTTGAG              
SspBI        2030  TGTACA               T^GTACA
             2573  TGTACA              
XcmI         1781  CCATGGATATGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2960  CCAAAAGCATGATGG     
XhoII        1285  AGATCC               [GA]^GATC[CT]
             4014  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwNI       ApaI        ApoI        AspHI       AvaI        
AvaII       BanII       BbsI        BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I