################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII Acc65I AflII AgeI Aor51HI AscI Asp718I AviII AvrII BamHI BbrPI BcgI BciVI BfrI BglII BlnI BsiWI BspDI BspHI BstBI BstEII BstPI ClaI DrdI Eco47III EcoNI EcoO65I EcoT22I FseI FspI KpnI MluI NdeI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PvuI PvuII RcaI SalI SbfI SfuI SgrAI SnaBI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccIII 67 TCCGGA T^CCGGA AclI 846 AACGTT AA^CGTT AhdI 4064 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2243 GTGCAC G^TGCAC ApaLI 2243 GTGCAC G^TGCAC AseI 4915 ATTAAT AT^TAAT AsnI 4915 ATTAAT AT^TAAT Asp700I 3097 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4064 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 865 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 3687 TGGCCA TGG^CCA BbeI 100 GGCGCC GGCGC^C BclI 1248 TGATCA T^GATCA BlpI 736 GCTGAGC GC^T[ACGT]AGC Bpu1102I 736 GCTGAGC GC^T[ACGT]AGC BsaAI 3398 TACGTG [CT]AC^GT[GA] BsaBI 1243 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 67 TCCGGA T^CCGGA BsmI 1949 GCATTC GAATGC(1/-1) BspEI 67 TCCGGA T^CCGGA BsrDI 4865 CATTGC GCAATG(2/0) BssHII 298 GCGCGC G^CGCGC CelII 736 GCTGAGC GC^T[ACGT]AGC CpoI 350 CGGACCG CG^G[AT]CCG Eam1105I 4064 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 814 GATATC GAT^ATC FbaI 1248 TGATCA T^GATCA HindIII 1108 AAGCTT A^AGCTT KasI 100 GGCGCC G^GCGCC KspI 312 CCGCGG CCGC^GG MamI 1243 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2471 CAATTG C^AATTG MluNI 3687 TGGCCA TGG^CCA MroI 67 TCCGGA T^CCGGA MscI 3687 TGGCCA TGG^CCA MunI 2471 CAATTG C^AATTG NaeI 376 GCCGGC GCC^GGC NarI 100 GGCGCC GG^CGCC NgoMIV 376 GCCGGC G^CCGGC NheI 4334 GCTAGC G^CTAGC PaeR7I 202 CTCGAG C^TCGAG PflFI 865 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2470 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1269 GGGTCCC [GA]G^G[AT]CC[CT] PshBI 4915 ATTAAT AT^TAAT Psp1406I 846 AACGTT AA^CGTT RsrII 350 CGGACCG CG^G[AT]CCG SacII 312 CCGCGG CCGC^GG SapI 2866 GAAGAGC GCTCTTC(1/4) SexAI 866 ACCAGGT A^CC[AT]GGT SfiI 549 GGCCGGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 100 GGCGCC GGC^GCC SmaI 3008 CCCGGG CCC^GGG SpeI 2247 ACTAGT A^CTAGT SspI 4908 AATATT AAT^ATT StuI 3693 AGGCCT AGG^CCT Tth111I 865 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2470 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 4462 TCTAGA T^CTAGA XhoI 202 CTCGAG C^TCGAG XmaCI 3008 CCCGGG C^CCGGG XmaI 3008 CCCGGG C^CCGGG XmnI 3097 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccI 2802 GTATAC GT^[AC][GT]AC 4206 GTATAC AcyI 55 GACGCC G[GA]^CG[CT]C 100 GGCGCC AflIII 2306 ACATGT A^C[GA][CT]GT 4844 ACATGT AlwI 1286 GATCC GGATC(4/5) 4015 GATCC AocI 2729 CCTTAGG CC^T[ACGT]AGG 4570 CCTTAGG BanI 100 GGCGCC G^G[CT][GA]CC 210 GGTGCC BbvCI 2673 CCTCAGC CCTCAGC(-5/-2) 4419 GCTGAGG BsaHI 55 GACGCC G[GA]^CG[CT]C 100 GGCGCC BsaWI 32 TCCGGT [AT]^CCGG[AT] 67 TCCGGA BsiEI 533 CGGCCG CG[GA][CT]^CG 548 CGGCCG BsmBI 53 GAGACG CGTCTC(1/5) 126 GAGACG Bsp1407I 2030 TGTACA T^GTACA 2573 TGTACA BspLU11I 2306 ACATGT A^CATGT 4844 ACATGT BspMI 563 GCAGGT ACCTGC(4/8) 887 GCAGGT BsrFI 207 GCCGGT [GA]^CCGG[CT] 376 GCCGGC BsrGI 2030 TGTACA T^GTACA 2573 TGTACA BssSI 767 CACGAG CACGAG(-5/-1) 2216 CACGAG Bst1107I 2802 GTATAC GTA^TAC 4206 GTATAC BstXI 1213 CCAGTGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1781 CCATGGATATGG BstYI 1285 AGATCC [GA]^GATC[CT] 4014 AGATCC BstZ17I 2802 GTATAC GTA^TAC 4206 GTATAC Bsu36I 2729 CCTTAGG CC^T[ACGT]AGG 4570 CCTTAGG Cfr10I 207 GCCGGT [GA]^CCGG[CT] 376 GCCGGC DraIII 1598 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 4297 CACATAGTG EagI 533 CGGCCG C^GGCCG 548 CGGCCG EclXI 533 CGGCCG C^GGCCG 548 CGGCCG Eco52I 533 CGGCCG C^GGCCG 548 CGGCCG Eco81I 2729 CCTTAGG CC^T[ACGT]AGG 4570 CCTTAGG EcoRI 784 GAATTC G^AATTC 2131 GAATTC HaeII 100 GGCGCC [GA]GCGC^[CT] 414 GGCGCT HgaI 55 GACGC GACGC(5/10) 128 GACGC Hin1I 55 GACGCC G[GA]^CG[CT]C 100 GGCGCC HpaI 4116 GTTAAC GTT^AAC 4195 GTTAAC MflI 1285 AGATCC [GA]^GATC[CT] 4014 AGATCC NcoI 1781 CCATGG C^CATGG 4897 CCATGG ScaI 1317 AGTACT AGT^ACT 1441 AGTACT SmlI 202 CTCGAG C^T[CT][GA]AG 1089 CTTGAG SspBI 2030 TGTACA T^GTACA 2573 TGTACA XcmI 1781 CCATGGATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2960 CCAAAAGCATGATGG XhoII 1285 AGATCC [GA]^GATC[CT] 4014 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwNI ApaI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I