################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Aor51HI AscI AseI AsnI AspEI BbrPI BcgI BciVI BclI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BssSI BstAPI BstBI ClaI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI HpaI KspI MamI MfeI MluI MroI MunI NcoI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI SacII SalI SexAI SfiI SfuI SnaBI SpeI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 741 GTATAC GT^[AC][GT]AC AflII 2553 CTTAAG C^TTAAG AflIII 2125 ACATGT A^C[GA][CT]GT Alw44I 3609 GTGCAC G^TGCAC ApaI 397 GGGCCC GGGCC^C ApaLI 3609 GTGCAC G^TGCAC Asp700I 204 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 3924 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2290 GGATCC G^GATCC BbeI 612 GGCGCC GGCGC^C BfrI 2553 CTTAAG C^TTAAG BlpI 2178 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2178 GCTGAGC GC^T[ACGT]AGC BspLU11I 2125 ACATGT A^CATGT BsrDI 3175 GCAATG GCAATG(2/0) BssHII 125 GCGCGC G^CGCGC Bst1107I 741 GTATAC GTA^TAC BstEII 3266 GGTCACC G^GT[ACGT]ACC BstPI 3266 GGTCACC G^GT[ACGT]ACC BstZ17I 741 GTATAC GTA^TAC CelII 2178 GCTGAGC GC^T[ACGT]AGC CpoI 700 CGGACCG CG^G[AT]CCG DraIII 3090 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 232 CCACGG C^C[GA][CT]GG EcoO65I 3266 GGTCACC G^GT[ACGT]ACC HindIII 2592 AAGCTT A^AGCTT KasI 612 GGCGCC G^GCGCC MaeII 2998 ACGT A^CGT NarI 612 GGCGCC GG^CGCC NdeI 1389 CATATG CA^TATG NheI 3879 GCTAGC G^CTAGC PflFI 3924 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 911 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 700 CGGACCG CG^G[AT]CCG SbfI 2906 CCTGCAGG CCTGCA^GG ScaI 874 AGTACT AGT^ACT SfoI 612 GGCGCC GGC^GCC SgrAI 131 CGCCGGCG C[GA]^CCGG[CT]G Sse8387I 2906 CCTGCAGG CCTGCA^GG Tth111I 3924 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3999 TCTAGA T^CTAGA XmnI 204 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 1770 GGTACC G^GTACC 3750 GGTACC AccII 126 CGCG CG^CG 174 CGCG AcsI 660 GAATTC [GA]^AATT[CT] 3413 GAATTC AocI 1236 CCTGAGG CC^T[ACGT]AGG 1920 CCTGAGG ApoI 660 GAATTC [GA]^AATT[CT] 3413 GAATTC Asp718I 1770 GGTACC G^GTACC 3750 GGTACC AvrII 1967 CCTAGG C^CTAGG 4050 CCTAGG BalI 832 TGGCCA TGG^CCA 1138 TGGCCA BglI 484 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2703 GCCCAGTTGGC BglII 536 AGATCT A^GATCT 3303 AGATCT BlnI 1967 CCTAGG C^CTAGG 4050 CCTAGG Bsp1407I 2128 TGTACA T^GTACA 3097 TGTACA BsrFI 132 GCCGGC [GA]^CCGG[CT] 584 GCCGGC BsrGI 2128 TGTACA T^GTACA 3097 TGTACA BstUI 126 CGCG CG^CG 174 CGCG BstXI 3 CCAAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3467 CCAGGGACCTGG Bsu36I 1236 CCTGAGG CC^T[ACGT]AGG 1920 CCTGAGG Cfr10I 132 GCCGGC [GA]^CCGG[CT] 584 GCCGGC Eco81I 1236 CCTGAGG CC^T[ACGT]AGG 1920 CCTGAGG EcoRI 660 GAATTC G^AATTC 3413 GAATTC KpnI 1770 GGTACC GGTAC^C 3750 GGTACC MluNI 832 TGGCCA TGG^CCA 1138 TGGCCA MscI 832 TGGCCA TGG^CCA 1138 TGGCCA MvnI 126 CGCG CG^CG 174 CGCG NaeI 132 GCCGGC GCC^GGC 584 GCCGGC NgoMIV 132 GCCGGC G^CCGGC 584 GCCGGC SphI 1087 GCATGC GCATG^C 1270 GCATGC SspBI 2128 TGTACA T^GTACA 3097 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII AviII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI