##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
BbrPI BcgI BciVI BclI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BssSI BstAPI
BstBI ClaI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI HpaI KspI
MamI MfeI MluI MroI MunI
NcoI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
SacII SalI SexAI SfiI SfuI
SnaBI SpeI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 741 GTATAC GT^[AC][GT]AC
AflII 2553 CTTAAG C^TTAAG
AflIII 2125 ACATGT A^C[GA][CT]GT
Alw44I 3609 GTGCAC G^TGCAC
ApaI 397 GGGCCC GGGCC^C
ApaLI 3609 GTGCAC G^TGCAC
Asp700I 204 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3924 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2290 GGATCC G^GATCC
BbeI 612 GGCGCC GGCGC^C
BfrI 2553 CTTAAG C^TTAAG
BlpI 2178 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2178 GCTGAGC GC^T[ACGT]AGC
BspLU11I 2125 ACATGT A^CATGT
BsrDI 3175 GCAATG GCAATG(2/0)
BssHII 125 GCGCGC G^CGCGC
Bst1107I 741 GTATAC GTA^TAC
BstEII 3266 GGTCACC G^GT[ACGT]ACC
BstPI 3266 GGTCACC G^GT[ACGT]ACC
BstZ17I 741 GTATAC GTA^TAC
CelII 2178 GCTGAGC GC^T[ACGT]AGC
CpoI 700 CGGACCG CG^G[AT]CCG
DraIII 3090 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 232 CCACGG C^C[GA][CT]GG
EcoO65I 3266 GGTCACC G^GT[ACGT]ACC
HindIII 2592 AAGCTT A^AGCTT
KasI 612 GGCGCC G^GCGCC
MaeII 2998 ACGT A^CGT
NarI 612 GGCGCC GG^CGCC
NdeI 1389 CATATG CA^TATG
NheI 3879 GCTAGC G^CTAGC
PflFI 3924 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 911 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 700 CGGACCG CG^G[AT]CCG
SbfI 2906 CCTGCAGG CCTGCA^GG
ScaI 874 AGTACT AGT^ACT
SfoI 612 GGCGCC GGC^GCC
SgrAI 131 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 2906 CCTGCAGG CCTGCA^GG
Tth111I 3924 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3999 TCTAGA T^CTAGA
XmnI 204 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1770 GGTACC G^GTACC
3750 GGTACC
AccII 126 CGCG CG^CG
174 CGCG
AcsI 660 GAATTC [GA]^AATT[CT]
3413 GAATTC
AocI 1236 CCTGAGG CC^T[ACGT]AGG
1920 CCTGAGG
ApoI 660 GAATTC [GA]^AATT[CT]
3413 GAATTC
Asp718I 1770 GGTACC G^GTACC
3750 GGTACC
AvrII 1967 CCTAGG C^CTAGG
4050 CCTAGG
BalI 832 TGGCCA TGG^CCA
1138 TGGCCA
BglI 484 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2703 GCCCAGTTGGC
BglII 536 AGATCT A^GATCT
3303 AGATCT
BlnI 1967 CCTAGG C^CTAGG
4050 CCTAGG
Bsp1407I 2128 TGTACA T^GTACA
3097 TGTACA
BsrFI 132 GCCGGC [GA]^CCGG[CT]
584 GCCGGC
BsrGI 2128 TGTACA T^GTACA
3097 TGTACA
BstUI 126 CGCG CG^CG
174 CGCG
BstXI 3 CCAAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3467 CCAGGGACCTGG
Bsu36I 1236 CCTGAGG CC^T[ACGT]AGG
1920 CCTGAGG
Cfr10I 132 GCCGGC [GA]^CCGG[CT]
584 GCCGGC
Eco81I 1236 CCTGAGG CC^T[ACGT]AGG
1920 CCTGAGG
EcoRI 660 GAATTC G^AATTC
3413 GAATTC
KpnI 1770 GGTACC GGTAC^C
3750 GGTACC
MluNI 832 TGGCCA TGG^CCA
1138 TGGCCA
MscI 832 TGGCCA TGG^CCA
1138 TGGCCA
MvnI 126 CGCG CG^CG
174 CGCG
NaeI 132 GCCGGC GCC^GGC
584 GCCGGC
NgoMIV 132 GCCGGC G^CCGGC
584 GCCGGC
SphI 1087 GCATGC GCATG^C
1270 GCATGC
SspBI 2128 TGTACA T^GTACA
3097 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII AviII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI