##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvrII BbrPI
BglII BlnI BsaAI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BstBI BstXI
ClaI CpoI DraI DrdI Eam1105I
EcoRI EcoT22I FseI HpaI KpnI
MfeI MunI NdeI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SalI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 1685 GTATAC GT^[AC][GT]AC
AccIII 1466 TCCGGA T^CCGGA
AflII 1360 CTTAAG C^TTAAG
AflIII 485 ACGCGT A^C[GA][CT]GT
AocI 3171 CCTGAGG CC^T[ACGT]AGG
Asp700I 4238 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 555 TGCGCA TGC^GCA
BamHI 2759 GGATCC G^GATCC
BciVI 4537 GGATAC GTATCC(6/5)
BfrI 1360 CTTAAG C^TTAAG
BsaBI 3512 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1466 TCCGGA T^CCGGA
BsmBI 1533 CGTCTC CGTCTC(1/5)
BspEI 1466 TCCGGA T^CCGGA
BsrDI 1650 GCAATG GCAATG(2/0)
Bst1107I 1685 GTATAC GTA^TAC
BstEII 1255 GGTTACC G^GT[ACGT]ACC
BstPI 1255 GGTTACC G^GT[ACGT]ACC
BstZ17I 1685 GTATAC GTA^TAC
Bsu36I 3171 CCTGAGG CC^T[ACGT]AGG
Eco81I 3171 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1255 GGTTACC G^GT[ACGT]ACC
EcoRV 1584 GATATC GAT^ATC
FspI 555 TGCGCA TGC^GCA
HindIII 3832 AAGCTT A^AGCTT
MamI 3512 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 485 ACGCGT A^CGCGT
MroI 1466 TCCGGA T^CCGGA
NaeI 3568 GCCGGC GCC^GGC
NgoMIV 3568 GCCGGC G^CCGGC
NotI 4329 GCGGCCGC GC^GGCCGC
NruI 4392 TCGCGA TCG^CGA
PaeR7I 4625 CTCGAG C^TCGAG
PflMI 852 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 304 CCTGCAGG CCTGCA^GG
ScaI 274 AGTACT AGT^ACT
SphI 1306 GCATGC GCATG^C
Sse8387I 304 CCTGCAGG CCTGCA^GG
Van91I 852 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 4625 CTCGAG C^TCGAG
XmnI 4238 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 2628 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3672 CAGCGCCTG
Aor51HI 3865 AGCGCT AGC^GCT
4055 AGCGCT
BcgI 3613 CGATGGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
694 GCAGAACCTTCG
BclI 1703 TGATCA T^GATCA
2135 TGATCA
BsaWI 104 TCCGGT [AT]^CCGG[AT]
1466 TCCGGA
BsrFI 2476 ACCGGC [GA]^CCGG[CT]
3568 GCCGGC
Cfr10I 2476 ACCGGC [GA]^CCGG[CT]
3568 GCCGGC
DraIII 728 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
4127 CACCGGGTG
Eco47III 3865 AGCGCT AGC^GCT
4055 AGCGCT
FbaI 1703 TGATCA T^GATCA
2135 TGATCA
NheI 1802 GCTAGC G^CTAGC
2402 GCTAGC
NspI 1145 ACATGC [GA]CATG^[CT]
1306 GCATGC
PpuMI 3251 AGGTCCT [GA]G^G[AT]CC[CT]
3321 AGGTCCT
PshAI 3246 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4359 GACGCAGGTC
SapI 3458 GCTCTTC GCTCTTC(1/4)
3738 GCTCTTC
SexAI 321 ACCTGGT A^CC[AT]GGT
1032 ACCAGGT
TfiI 427 GAATC G^A[AT]TC
1529 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrI
BssHII BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV PleI PstI PvuII RcaI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI