################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI AvrII BbrPI BglII BlnI BsaAI BsiWI Bsp1407I BspDI BspLU11I BsrGI BstBI BstXI ClaI CpoI DraI DrdI Eam1105I EcoRI EcoT22I FseI HpaI KpnI MfeI MunI NdeI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SalI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 1685 GTATAC GT^[AC][GT]AC AccIII 1466 TCCGGA T^CCGGA AflII 1360 CTTAAG C^TTAAG AflIII 485 ACGCGT A^C[GA][CT]GT AocI 3171 CCTGAGG CC^T[ACGT]AGG Asp700I 4238 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 555 TGCGCA TGC^GCA BamHI 2759 GGATCC G^GATCC BciVI 4537 GGATAC GTATCC(6/5) BfrI 1360 CTTAAG C^TTAAG BsaBI 3512 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1466 TCCGGA T^CCGGA BsmBI 1533 CGTCTC CGTCTC(1/5) BspEI 1466 TCCGGA T^CCGGA BsrDI 1650 GCAATG GCAATG(2/0) Bst1107I 1685 GTATAC GTA^TAC BstEII 1255 GGTTACC G^GT[ACGT]ACC BstPI 1255 GGTTACC G^GT[ACGT]ACC BstZ17I 1685 GTATAC GTA^TAC Bsu36I 3171 CCTGAGG CC^T[ACGT]AGG Eco81I 3171 CCTGAGG CC^T[ACGT]AGG EcoO65I 1255 GGTTACC G^GT[ACGT]ACC EcoRV 1584 GATATC GAT^ATC FspI 555 TGCGCA TGC^GCA HindIII 3832 AAGCTT A^AGCTT MamI 3512 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 485 ACGCGT A^CGCGT MroI 1466 TCCGGA T^CCGGA NaeI 3568 GCCGGC GCC^GGC NgoMIV 3568 GCCGGC G^CCGGC NotI 4329 GCGGCCGC GC^GGCCGC NruI 4392 TCGCGA TCG^CGA PaeR7I 4625 CTCGAG C^TCGAG PflMI 852 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SbfI 304 CCTGCAGG CCTGCA^GG ScaI 274 AGTACT AGT^ACT SphI 1306 GCATGC GCATG^C Sse8387I 304 CCTGCAGG CCTGCA^GG Van91I 852 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 4625 CTCGAG C^TCGAG XmnI 4238 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwNI 2628 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 3672 CAGCGCCTG Aor51HI 3865 AGCGCT AGC^GCT 4055 AGCGCT BcgI 3613 CGATGGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 694 GCAGAACCTTCG BclI 1703 TGATCA T^GATCA 2135 TGATCA BsaWI 104 TCCGGT [AT]^CCGG[AT] 1466 TCCGGA BsrFI 2476 ACCGGC [GA]^CCGG[CT] 3568 GCCGGC Cfr10I 2476 ACCGGC [GA]^CCGG[CT] 3568 GCCGGC DraIII 728 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG 4127 CACCGGGTG Eco47III 3865 AGCGCT AGC^GCT 4055 AGCGCT FbaI 1703 TGATCA T^GATCA 2135 TGATCA NheI 1802 GCTAGC G^CTAGC 2402 GCTAGC NspI 1145 ACATGC [GA]CATG^[CT] 1306 GCATGC PpuMI 3251 AGGTCCT [GA]G^G[AT]CC[CT] 3321 AGGTCCT PshAI 3246 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4359 GACGCAGGTC SapI 3458 GCTCTTC GCTCTTC(1/4) 3738 GCTCTTC SexAI 321 ACCTGGT A^CC[AT]GGT 1032 ACCAGGT TfiI 427 GAATC G^A[AT]TC 1529 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 139 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrBI BsrI BssHII BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV PleI PstI PvuII RcaI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI