##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
Alw44I ApaLI AscI AseI AsnI
Asp718I AviII BamHI BclI BsaBI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BsrBI BssHII Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DraIII
EagI EclXI Eco52I EcoRI EcoRV
FbaI FseI FspI HgaI HpaI
KpnI KspI MamI MluI MroI
MvnI NaeI NdeI NgoMIV NotI
NruI NspV PacI PmeI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI Sse8387I SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 1451 GTCTAC GT^[AC][GT]AC
AcyI 3255 GGCGCC G[GA]^CG[CT]C
AflII 4222 CTTAAG C^TTAAG
AgeI 3789 ACCGGT A^CCGGT
AhdI 2663 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3225 AGCGCT AGC^GCT
AspEI 2663 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3391 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4211 CCTAGG C^CTAGG
BalI 556 TGGCCA TGG^CCA
BbeI 3255 GGCGCC GGCGC^C
BfrI 4222 CTTAAG C^TTAAG
BglII 1773 AGATCT A^GATCT
BlnI 4211 CCTAGG C^CTAGG
BlpI 3687 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3687 GCTCAGC GC^T[ACGT]AGC
BsaHI 3255 GGCGCC G[GA]^CG[CT]C
BsaWI 3789 ACCGGT [AT]^CCGG[AT]
BsmBI 3811 GAGACG CGTCTC(1/5)
BspHI 1141 TCATGA T^CATGA
BsrFI 3789 ACCGGT [GA]^CCGG[CT]
BssSI 527 CACGAG CACGAG(-5/-1)
BstEII 1028 GGTCACC G^GT[ACGT]ACC
BstPI 1028 GGTCACC G^GT[ACGT]ACC
CelII 3687 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3789 ACCGGT [GA]^CCGG[CT]
DraI 1702 TTTAAA TTT^AAA
Eam1105I 2663 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3225 AGCGCT AGC^GCT
EcoO65I 1028 GGTCACC G^GT[ACGT]ACC
EcoT22I 1956 ATGCAT ATGCA^T
Hin1I 3255 GGCGCC G[GA]^CG[CT]C
HindIII 2320 AAGCTT A^AGCTT
KasI 3255 GGCGCC G^GCGCC
MfeI 513 CAATTG C^AATTG
MluNI 556 TGGCCA TGG^CCA
MscI 556 TGGCCA TGG^CCA
MunI 513 CAATTG C^AATTG
NarI 3255 GGCGCC GG^CGCC
NcoI 2265 CCATGG C^CATGG
NheI 2646 GCTAGC G^CTAGC
NsiI 1956 ATGCAT ATGCA^T
PaeR7I 3423 CTCGAG C^TCGAG
PflFI 3391 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3789 ACCGGT A^CCGGT
PshAI 3494 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1141 TCATGA T^CATGA
SfoI 3255 GGCGCC GGC^GCC
SmaI 388 CCCGGG CCC^GGG
SpeI 3486 ACTAGT A^CTAGT
Tth111I 3391 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3423 CTCGAG C^TCGAG
XmaCI 388 CCCGGG C^CCGGG
XmaI 388 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 2086 AAATTT [GA]^AATT[CT]
4519 AAATTT
AflIII 1357 ACACGT A^C[GA][CT]GT
2786 ACGTGT
ApaI 1433 GGGCCC GGGCC^C
4014 GGGCCC
ApoI 2086 AAATTT [GA]^AATT[CT]
4519 AAATTT
Asp700I 3199 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4757 GAAGCCCTTC
BbrPI 1358 CACGTG CAC^GTG
1802 CACGTG
BciVI 1635 GGATAC GTATCC(6/5)
4438 GTATCC
BsaAI 1358 CACGTG [CT]AC^GT[GA]
1802 CACGTG
Bsp1407I 3311 TGTACA T^GTACA
4354 TGTACA
BsrDI 292 GCAATG GCAATG(2/0)
1459 GCAATG
BsrGI 3311 TGTACA T^GTACA
4354 TGTACA
DrdI 151 GACACCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4037 GACAACTAGGTC
DsaI 2265 CCATGG C^C[GA][CT]GG
3658 CCACGG
FauI 113 CCCGC CCCGC(4/6)
607 CCCGC
PmaCI 1358 CACGTG CAC^GTG
1802 CACGTG
PmlI 1358 CACGTG CAC^GTG
1802 CACGTG
SphI 1015 GCATGC GCATG^C
1088 GCATGC
SspBI 3311 TGTACA T^GTACA
4354 TGTACA
XmnI 3199 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4757 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII