################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AccII AccIII AclI Alw44I ApaLI AscI AseI AsnI Asp718I AviII BamHI BclI BsaBI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraIII EagI EclXI Eco52I EcoRI EcoRV FbaI FseI FspI HgaI HpaI KpnI KspI MamI MluI MroI MvnI NaeI NdeI NgoMIV NotI NruI NspV PacI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 1451 GTCTAC GT^[AC][GT]AC AcyI 3255 GGCGCC G[GA]^CG[CT]C AflII 4222 CTTAAG C^TTAAG AgeI 3789 ACCGGT A^CCGGT AhdI 2663 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3225 AGCGCT AGC^GCT AspEI 2663 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3391 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4211 CCTAGG C^CTAGG BalI 556 TGGCCA TGG^CCA BbeI 3255 GGCGCC GGCGC^C BfrI 4222 CTTAAG C^TTAAG BglII 1773 AGATCT A^GATCT BlnI 4211 CCTAGG C^CTAGG BlpI 3687 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3687 GCTCAGC GC^T[ACGT]AGC BsaHI 3255 GGCGCC G[GA]^CG[CT]C BsaWI 3789 ACCGGT [AT]^CCGG[AT] BsmBI 3811 GAGACG CGTCTC(1/5) BspHI 1141 TCATGA T^CATGA BsrFI 3789 ACCGGT [GA]^CCGG[CT] BssSI 527 CACGAG CACGAG(-5/-1) BstEII 1028 GGTCACC G^GT[ACGT]ACC BstPI 1028 GGTCACC G^GT[ACGT]ACC CelII 3687 GCTCAGC GC^T[ACGT]AGC Cfr10I 3789 ACCGGT [GA]^CCGG[CT] DraI 1702 TTTAAA TTT^AAA Eam1105I 2663 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3225 AGCGCT AGC^GCT EcoO65I 1028 GGTCACC G^GT[ACGT]ACC EcoT22I 1956 ATGCAT ATGCA^T Hin1I 3255 GGCGCC G[GA]^CG[CT]C HindIII 2320 AAGCTT A^AGCTT KasI 3255 GGCGCC G^GCGCC MfeI 513 CAATTG C^AATTG MluNI 556 TGGCCA TGG^CCA MscI 556 TGGCCA TGG^CCA MunI 513 CAATTG C^AATTG NarI 3255 GGCGCC GG^CGCC NcoI 2265 CCATGG C^CATGG NheI 2646 GCTAGC G^CTAGC NsiI 1956 ATGCAT ATGCA^T PaeR7I 3423 CTCGAG C^TCGAG PflFI 3391 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3789 ACCGGT A^CCGGT PshAI 3494 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1141 TCATGA T^CATGA SfoI 3255 GGCGCC GGC^GCC SmaI 388 CCCGGG CCC^GGG SpeI 3486 ACTAGT A^CTAGT Tth111I 3391 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3423 CTCGAG C^TCGAG XmaCI 388 CCCGGG C^CCGGG XmaI 388 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 2086 AAATTT [GA]^AATT[CT] 4519 AAATTT AflIII 1357 ACACGT A^C[GA][CT]GT 2786 ACGTGT ApaI 1433 GGGCCC GGGCC^C 4014 GGGCCC ApoI 2086 AAATTT [GA]^AATT[CT] 4519 AAATTT Asp700I 3199 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4757 GAAGCCCTTC BbrPI 1358 CACGTG CAC^GTG 1802 CACGTG BciVI 1635 GGATAC GTATCC(6/5) 4438 GTATCC BsaAI 1358 CACGTG [CT]AC^GT[GA] 1802 CACGTG Bsp1407I 3311 TGTACA T^GTACA 4354 TGTACA BsrDI 292 GCAATG GCAATG(2/0) 1459 GCAATG BsrGI 3311 TGTACA T^GTACA 4354 TGTACA DrdI 151 GACACCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4037 GACAACTAGGTC DsaI 2265 CCATGG C^C[GA][CT]GG 3658 CCACGG FauI 113 CCCGC CCCGC(4/6) 607 CCCGC PmaCI 1358 CACGTG CAC^GTG 1802 CACGTG PmlI 1358 CACGTG CAC^GTG 1802 CACGTG SphI 1015 GCATGC GCATG^C 1088 GCATGC SspBI 3311 TGTACA T^GTACA 4354 TGTACA XmnI 3199 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4757 GAAGCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII