##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AccI AclI AflII AgeI Alw44I
ApaI ApaLI AscI AspHI AvaI
AviII AvrII BbeI BbrPI BcgI
BciVI BfrI BglI BlnI BsaAI
BsaBI BsgI BsiHKAI BsiWI BsmBI
BsoBI BspDI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
DraI DraII EcoO109I EcoO65I FseI
FspI HpaI KasI MamI MluI
NarI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PstI PvuI SacI SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I StuI
SwaI Van91I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 209 GACGTC GACGT^C
AccII 235 CGCG CG^CG
AcyI 209 GACGTC G[GA]^CG[CT]C
AhdI 100 GACGGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3076 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2600 CCTCAGG CC^T[ACGT]AGG
Aor51HI 740 AGCGCT AGC^GCT
AseI 3737 ATTAAT AT^TAAT
AsnI 3737 ATTAAT AT^TAAT
AspEI 100 GACGGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1498 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2723 GGATCC G^GATCC
BbvCI 23 CCTCAGC CCTCAGC(-5/-2)
BlpI 1679 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1679 GCTCAGC GC^T[ACGT]AGC
BsaHI 209 GACGTC G[GA]^CG[CT]C
BsmI 2179 GCATTC GAATGC(1/-1)
BspLU11I 618 ACATGT A^CATGT
BsrBI 81 CCGCTC CCGCTC(-3/-3)
BsrFI 239 GCCGGC [GA]^CCGG[CT]
BstUI 235 CGCG CG^CG
Bsu36I 2600 CCTCAGG CC^T[ACGT]AGG
CelII 1679 GCTCAGC GC^T[ACGT]AGC
CfoI 741 GCGC GCG^C
Cfr10I 239 GCCGGC [GA]^CCGG[CT]
CpoI 114 CGGTCCG CG^G[AT]CCG
DraIII 582 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 640 GACAGCTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 237 CGGCCG C^GGCCG
Eam1105I 100 GACGGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 237 CGGCCG C^GGCCG
Eco47III 740 AGCGCT AGC^GCT
Eco52I 237 CGGCCG C^GGCCG
Eco81I 2600 CCTCAGG CC^T[ACGT]AGG
EcoRI 3647 GAATTC G^AATTC
EcoT22I 466 ATGCAT ATGCA^T
HaeII 740 AGCGCT [GA]GCGC^[CT]
HgaI 294 GCGTC GACGC(5/10)
HhaI 741 GCGC GCG^C
Hin1I 209 GACGTC G[GA]^CG[CT]C
HinP1I 741 GCGC G^CGC
HincII 3455 GTCAAC GT[CT]^[GA]AC
HindII 3455 GTCAAC GT[CT]^[GA]AC
HindIII 1568 AAGCTT A^AGCTT
KspI 234 CCGCGG CCGC^GG
MfeI 2964 CAATTG C^AATTG
MunI 2964 CAATTG C^AATTG
MvnI 235 CGCG CG^CG
NaeI 239 GCCGGC GCC^GGC
NgoMIV 239 GCCGGC G^CCGGC
NsiI 466 ATGCAT ATGCA^T
PflFI 1498 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 431 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3737 ATTAAT AT^TAAT
RsrII 114 CGGTCCG CG^G[AT]CCG
SacII 234 CCGCGG CCGC^GG
SapI 3215 GCTCTTC GCTCTTC(1/4)
SphI 2956 GCATGC GCATG^C
Tth111I 1498 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3509 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 378 GGTACC G^GTACC
1803 GGTACC
AccIII 117 TCCGGA T^CCGGA
1071 TCCGGA
AflIII 618 ACATGT A^C[GA][CT]GT
733 ACACGT
Asp718I 378 GGTACC G^GTACC
1803 GGTACC
BclI 1868 TGATCA T^GATCA
3569 TGATCA
BsaWI 117 TCCGGA [AT]^CCGG[AT]
1071 TCCGGA
BseAI 117 TCCGGA T^CCGGA
1071 TCCGGA
BsiEI 201 CGGTCG CG[GA][CT]^CG
237 CGGCCG
BspEI 117 TCCGGA T^CCGGA
1071 TCCGGA
BspMI 677 GCAGGT ACCTGC(4/8)
1889 GCAGGT
BsrDI 463 GCAATG GCAATG(2/0)
1780 GCAATG
BssSI 41 CTCGTG CACGAG(-5/-1)
3631 CTCGTG
DsaI 222 CCGTGG C^C[GA][CT]GG
234 CCGCGG
EarI 3216 CTCTTC CTCTTC(1/4)
3431 GAAGAG
FauI 80 CCCGC CCCGC(4/6)
3177 GCGGG
FbaI 1868 TGATCA T^GATCA
3569 TGATCA
KpnI 378 GGTACC GGTAC^C
1803 GGTACC
Ksp632I 3216 CTCTTC CTCTTC(1/4)
3431 GAAGAG
MroI 117 TCCGGA T^CCGGA
1071 TCCGGA
MslI 619 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3863 CATGGAAATG
ScaI 2075 AGTACT AGT^ACT
2096 AGTACT
SfcI 801 CTACAG C^T[GA][CT]AG
1793 CTATAG
SmlI 2118 CTCAAG C^T[CT][GA]AG
2637 CTCAAG
SpeI 1932 ACTAGT A^CTAGT
3443 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspHI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EcoNI EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI