##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Aor51HI ApaI AscI
Asp718I AviII BalI BbeI BcgI
BclI BfrI BglI BseAI BspDI
BspEI BspMI BssHII Bst1107I BstAPI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HpaI KasI
KpnI KspI MluNI MroI MscI
NarI NcoI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AhdI 2639 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2867 GTGCAC G^TGCAC
AocI 446 CCTCAGG CC^T[ACGT]AGG
ApaLI 2867 GTGCAC G^TGCAC
AseI 1055 ATTAAT AT^TAAT
AsnI 1055 ATTAAT AT^TAAT
AspEI 2639 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3177 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2238 CCTAGG C^CTAGG
BbrPI 63 CACGTG CAC^GTG
BbvCI 3590 CCTCAGC CCTCAGC(-5/-2)
BlnI 2238 CCTAGG C^CTAGG
BsaAI 63 CACGTG [CT]AC^GT[GA]
BsaBI 365 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4284 ACCGGA [AT]^CCGG[AT]
BsiEI 2388 CGGTCG CG[GA][CT]^CG
BsiWI 2694 CGTACG C^GTACG
BsmI 4174 GCATTC GAATGC(1/-1)
Bsp1407I 1822 TGTACA T^GTACA
BspHI 2784 TCATGA T^CATGA
BsrDI 3996 GCAATG GCAATG(2/0)
BsrGI 1822 TGTACA T^GTACA
BstBI 1077 TTCGAA TT^CGAA
BstXI 1369 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 446 CCTCAGG CC^T[ACGT]AGG
CfoI 2676 GCGC GCG^C
DraIII 2167 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3133 CCGTGG C^C[GA][CT]GG
Eam1105I 2639 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 446 CCTCAGG CC^T[ACGT]AGG
EcoRI 3249 GAATTC G^AATTC
HhaI 2676 GCGC GCG^C
HinP1I 2676 GCGC G^CGC
HincII 638 GTCAAC GT[CT]^[GA]AC
HindII 638 GTCAAC GT[CT]^[GA]AC
HindIII 2344 AAGCTT A^AGCTT
MamI 365 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2399 ACGCGT A^CGCGT
NaeI 2748 GCCGGC GCC^GGC
NgoMIV 2748 GCCGGC G^CCGGC
NspV 1077 TTCGAA TT^CGAA
PflFI 3177 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 63 CACGTG CAC^GTG
PmlI 63 CACGTG CAC^GTG
PshAI 1612 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1055 ATTAAT AT^TAAT
PstI 2212 CTGCAG CTGCA^G
RcaI 2784 TCATGA T^CATGA
SfuI 1077 TTCGAA TT^CGAA
SspBI 1822 TGTACA T^GTACA
SspI 1975 AATATT AAT^ATT
Tth111I 3177 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BamHI 1282 GGATCC G^GATCC
2546 GGATCC
BanI 1485 GGCACC G^G[CT][GA]CC
3710 GGCACC
BciVI 296 GGATAC GTATCC(6/5)
1937 GGATAC
BglII 716 AGATCT A^GATCT
3203 AGATCT
BlpI 573 GCTTAGC GC^T[ACGT]AGC
1886 GCTCAGC
BpmI 3274 CTCCAG CTGGAG(16/14)
944 CTGGAG
Bpu1102I 573 GCTTAGC GC^T[ACGT]AGC
1886 GCTCAGC
BsgI 2141 CTGCAC GTGCAG(16/14)
4215 GTGCAG
BsmBI 4191 GAGACG CGTCTC(1/5)
4222 GAGACG
BspLU11I 3808 ACATGT A^CATGT
4447 ACATGT
BsrBI 230 GAGCGG CCGCTC(-3/-3)
3676 GAGCGG
BssSI 1091 CACGAG CACGAG(-5/-1)
3767 CACGAG
CelII 573 GCTTAGC GC^T[ACGT]AGC
1886 GCTCAGC
DraII 383 AGGTCCC [GA]G^G[ACGT]CC[CT]
2953 AGGACCT
EaeI 2384 TGGCCG [CT]^GGCC[GA]
4000 TGGCCG
EcoNI 2258 CCTTAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2466 CCTGCTACAGG
EcoO109I 383 AGGTCCC [GA]G^G[ACGT]CC[CT]
2953 AGGACCT
FauI 490 CCCGC CCCGC(4/6)
501 CCCGC
MfeI 2412 CAATTG C^AATTG
2682 CAATTG
MunI 2412 CAATTG C^AATTG
2682 CAATTG
NdeI 1223 CATATG CA^TATG
1958 CATATG
PflMI 800 CCACAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
916 CCAAAATGTGG
PpuMI 383 AGGTCCC [GA]G^G[AT]CC[CT]
2953 AGGACCT
SfcI 2212 CTGCAG C^T[GA][CT]AG
2470 CTACAG
SmaI 240 CCCGGG CCC^GGG
2427 CCCGGG
Van91I 800 CCACAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
916 CCAAAATGTGG
XbaI 1493 TCTAGA T^CTAGA
4259 TCTAGA
XmaCI 240 CCCGGG C^CCGGG
2427 CCCGGG
XmaI 240 CCCGGG C^CCGGG
2427 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI Hin1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI