################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AccIII AclI Alw44I Aor51HI ApaLI AscI AseI AsnI AvrII BbeI BcgI BlnI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrGI BssHII BstBI BstEII BstPI ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FseI HaeII HincII HindII HpaI KasI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PmeI PshBI Psp1406I PvuI RsrII SacI SalI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 2679 GACGTC GACGT^C AccI 1001 GTATAC GT^[AC][GT]AC AcsI 1182 AAATTT [GA]^AATT[CT] AflII 3913 CTTAAG C^TTAAG AgeI 35 ACCGGT A^CCGGT ApoI 1182 AAATTT [GA]^AATT[CT] Asp700I 3071 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 570 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2245 TGCGCA TGC^GCA BbrPI 1167 CACGTG CAC^GTG BclI 3122 TGATCA T^GATCA BfrI 3913 CTTAAG C^TTAAG BglII 2965 AGATCT A^GATCT BlpI 3102 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3102 GCTCAGC GC^T[ACGT]AGC BsaWI 35 ACCGGT [AT]^CCGG[AT] BsmBI 1708 CGTCTC CGTCTC(1/5) BsmI 2471 GAATGC GAATGC(1/-1) BspHI 1858 TCATGA T^CATGA BspLU11I 551 ACATGT A^CATGT BsrDI 3251 CATTGC GCAATG(2/0) BssSI 1200 CTCGTG CACGAG(-5/-1) Bst1107I 1001 GTATAC GTA^TAC BstZ17I 1001 GTATAC GTA^TAC CelII 3102 GCTCAGC GC^T[ACGT]AGC EcoT22I 4164 ATGCAT ATGCA^T FbaI 3122 TGATCA T^GATCA FspI 2245 TGCGCA TGC^GCA KspI 1076 CCGCGG CCGC^GG NsiI 4164 ATGCAT ATGCA^T PaeR7I 3334 CTCGAG C^TCGAG PflFI 570 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 35 ACCGGT A^CCGGT PmaCI 1167 CACGTG CAC^GTG PmlI 1167 CACGTG CAC^GTG RcaI 1858 TCATGA T^CATGA SacII 1076 CCGCGG CCGC^GG SbfI 3895 CCTGCAGG CCTGCA^GG SfiI 3940 GGCCCTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 418 CCCGGG CCC^GGG Sse8387I 3895 CCTGCAGG CCTGCA^GG Tth111I 570 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3334 CTCGAG C^TCGAG XmaCI 418 CCCGGG C^CCGGG XmaI 418 CCCGGG C^CCGGG XmnI 3071 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 789 GGTACC G^GTACC 1954 GGTACC AflIII 551 ACATGT A^C[GA][CT]GT 1166 ACACGT AhdI 706 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3388 GACAGACTGTC AocI 1772 CCTGAGG CC^T[ACGT]AGG 4203 CCTCAGG ApaI 1031 GGGCCC GGGCC^C 1485 GGGCCC Asp718I 789 GGTACC G^GTACC 1954 GGTACC AspEI 706 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3388 GACAGACTGTC AspHI 3240 GTGCTC G[AT]GC[AT]^C 3976 GAGCAC BbvCI 3783 CCTCAGC CCTCAGC(-5/-2) 4186 CCTCAGC BciVI 3128 GGATAC GTATCC(6/5) 1903 GTATCC BsaAI 1167 CACGTG [CT]AC^GT[GA] 2124 TACGTG BsaBI 2288 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3176 GATCAACATC BsiHKAI 3240 GTGCTC G[AT]GC[AT]^C 3976 GAGCAC BsrBI 1237 CCGCTC CCGCTC(-3/-3) 972 GAGCGG BstAPI 346 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1544 GCAGCCCCTGC Bsu36I 1772 CCTGAGG CC^T[ACGT]AGG 4203 CCTCAGG DraIII 452 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 477 CACTCGGTG Eam1105I 706 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3388 GACAGACTGTC Eco81I 1772 CCTGAGG CC^T[ACGT]AGG 4203 CCTCAGG HindIII 226 AAGCTT A^AGCTT 2160 AAGCTT KpnI 789 GGTACC GGTAC^C 1954 GGTACC MamI 2288 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3176 GATCAACATC NspI 305 GCATGT [GA]CATG^[CT] 551 ACATGT ScaI 3073 AGTACT AGT^ACT 3508 AGTACT SexAI 2203 ACCTGGT A^CC[AT]GGT 2872 ACCTGGT StuI 3809 AGGCCT AGG^CCT 3843 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaI AvaII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PshAI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII