##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AclI Alw44I Aor51HI ApaLI
AscI AseI AsnI AvrII BbeI
BcgI BlnI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
BstBI BstEII BstPI ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FseI HaeII
HincII HindII HpaI KasI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PmeI PshBI
Psp1406I PvuI RsrII SacI SalI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 2679 GACGTC GACGT^C
AccI 1001 GTATAC GT^[AC][GT]AC
AcsI 1182 AAATTT [GA]^AATT[CT]
AflII 3913 CTTAAG C^TTAAG
AgeI 35 ACCGGT A^CCGGT
ApoI 1182 AAATTT [GA]^AATT[CT]
Asp700I 3071 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 570 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2245 TGCGCA TGC^GCA
BbrPI 1167 CACGTG CAC^GTG
BclI 3122 TGATCA T^GATCA
BfrI 3913 CTTAAG C^TTAAG
BglII 2965 AGATCT A^GATCT
BlpI 3102 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3102 GCTCAGC GC^T[ACGT]AGC
BsaWI 35 ACCGGT [AT]^CCGG[AT]
BsmBI 1708 CGTCTC CGTCTC(1/5)
BsmI 2471 GAATGC GAATGC(1/-1)
BspHI 1858 TCATGA T^CATGA
BspLU11I 551 ACATGT A^CATGT
BsrDI 3251 CATTGC GCAATG(2/0)
BssSI 1200 CTCGTG CACGAG(-5/-1)
Bst1107I 1001 GTATAC GTA^TAC
BstZ17I 1001 GTATAC GTA^TAC
CelII 3102 GCTCAGC GC^T[ACGT]AGC
EcoT22I 4164 ATGCAT ATGCA^T
FbaI 3122 TGATCA T^GATCA
FspI 2245 TGCGCA TGC^GCA
KspI 1076 CCGCGG CCGC^GG
NsiI 4164 ATGCAT ATGCA^T
PaeR7I 3334 CTCGAG C^TCGAG
PflFI 570 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 35 ACCGGT A^CCGGT
PmaCI 1167 CACGTG CAC^GTG
PmlI 1167 CACGTG CAC^GTG
RcaI 1858 TCATGA T^CATGA
SacII 1076 CCGCGG CCGC^GG
SbfI 3895 CCTGCAGG CCTGCA^GG
SfiI 3940 GGCCCTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 418 CCCGGG CCC^GGG
Sse8387I 3895 CCTGCAGG CCTGCA^GG
Tth111I 570 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3334 CTCGAG C^TCGAG
XmaCI 418 CCCGGG C^CCGGG
XmaI 418 CCCGGG C^CCGGG
XmnI 3071 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 789 GGTACC G^GTACC
1954 GGTACC
AflIII 551 ACATGT A^C[GA][CT]GT
1166 ACACGT
AhdI 706 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3388 GACAGACTGTC
AocI 1772 CCTGAGG CC^T[ACGT]AGG
4203 CCTCAGG
ApaI 1031 GGGCCC GGGCC^C
1485 GGGCCC
Asp718I 789 GGTACC G^GTACC
1954 GGTACC
AspEI 706 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3388 GACAGACTGTC
AspHI 3240 GTGCTC G[AT]GC[AT]^C
3976 GAGCAC
BbvCI 3783 CCTCAGC CCTCAGC(-5/-2)
4186 CCTCAGC
BciVI 3128 GGATAC GTATCC(6/5)
1903 GTATCC
BsaAI 1167 CACGTG [CT]AC^GT[GA]
2124 TACGTG
BsaBI 2288 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3176 GATCAACATC
BsiHKAI 3240 GTGCTC G[AT]GC[AT]^C
3976 GAGCAC
BsrBI 1237 CCGCTC CCGCTC(-3/-3)
972 GAGCGG
BstAPI 346 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1544 GCAGCCCCTGC
Bsu36I 1772 CCTGAGG CC^T[ACGT]AGG
4203 CCTCAGG
DraIII 452 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
477 CACTCGGTG
Eam1105I 706 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3388 GACAGACTGTC
Eco81I 1772 CCTGAGG CC^T[ACGT]AGG
4203 CCTCAGG
HindIII 226 AAGCTT A^AGCTT
2160 AAGCTT
KpnI 789 GGTACC GGTAC^C
1954 GGTACC
MamI 2288 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3176 GATCAACATC
NspI 305 GCATGT [GA]CATG^[CT]
551 ACATGT
ScaI 3073 AGTACT AGT^ACT
3508 AGTACT
SexAI 2203 ACCTGGT A^CC[AT]GGT
2872 ACCTGGT
StuI 3809 AGGCCT AGG^CCT
3843 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PshAI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII