################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AspI AviII BcgI BfrI BsaBI BseAI BsiWI BspDI BspEI BssHII Bst1107I BstZ17I ClaI CpoI DrdI Eam1105I EcoRV FseI FspI MamI MluI MroI NaeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI SfiI SgrAI SmaI Sse8387I Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 4186 GGTACC G^GTACC Asp718I 4186 GGTACC G^GTACC BbeI 409 GGCGCC GGCGC^C BbvCI 4214 GCTGAGG CCTCAGC(-5/-2) BglI 3873 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 180 GCTCAGC GC^T[ACGT]AGC Bpu1102I 180 GCTCAGC GC^T[ACGT]AGC BsaWI 1682 ACCGGA [AT]^CCGG[AT] BsmBI 4979 CGTCTC CGTCTC(1/5) BsmI 2405 GAATGC GAATGC(1/-1) BspHI 1340 TCATGA T^CATGA BssSI 5508 CACGAG CACGAG(-5/-1) BstAPI 2167 GCACTTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 4694 GGTGACC G^GT[ACGT]ACC BstPI 4694 GGTGACC G^GT[ACGT]ACC CelII 180 GCTCAGC GC^T[ACGT]AGC EagI 534 CGGCCG C^GGCCG EclXI 534 CGGCCG C^GGCCG Eco52I 534 CGGCCG C^GGCCG EcoO65I 4694 GGTGACC G^GT[ACGT]ACC EcoRI 1840 GAATTC G^AATTC EcoT22I 3760 ATGCAT ATGCA^T HpaI 3734 GTTAAC GTT^AAC KasI 409 GGCGCC G^GCGCC KpnI 4186 GGTACC GGTAC^C NarI 409 GGCGCC GG^CGCC NsiI 3760 ATGCAT ATGCA^T RcaI 1340 TCATGA T^CATGA ScaI 3494 AGTACT AGT^ACT SfoI 409 GGCGCC GGC^GCC SnaBI 4579 TACGTA TAC^GTA SpeI 3117 ACTAGT A^CTAGT SspI 3612 AATATT AAT^ATT StuI 3770 AGGCCT AGG^CCT SwaI 5690 ATTTAAAT ATTT^AAAT XcmI 4699 CCAAGGAATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AcyI 315 GACGCC G[GA]^CG[CT]C 409 GGCGCC AocI 3876 CCTCAGG CC^T[ACGT]AGG 5184 CCTCAGG ApaI 148 GGGCCC GGGCC^C 1460 GGGCCC AvaI 219 CTCGGG C^[CT]CG[GA]G 905 CTCGGG AvrII 585 CCTAGG C^CTAGG 2765 CCTAGG BalI 2042 TGGCCA TGG^CCA 4553 TGGCCA BamHI 893 GGATCC G^GATCC 5078 GGATCC BbrPI 2531 CACGTG CAC^GTG 3814 CACGTG BciVI 621 GGATAC GTATCC(6/5) 3665 GTATCC BclI 677 TGATCA T^GATCA 2934 TGATCA BlnI 585 CCTAGG C^CTAGG 2765 CCTAGG BsaHI 315 GACGCC G[GA]^CG[CT]C 409 GGCGCC BsiEI 9 CGGTCG CG[GA][CT]^CG 534 CGGCCG BsoBI 219 CTCGGG C^[CT]CG[GA]G 905 CTCGGG Bsp1407I 833 TGTACA T^GTACA 1884 TGTACA BsrFI 967 GCCGGT [GA]^CCGG[CT] 5318 GCCGGT BsrGI 833 TGTACA T^GTACA 1884 TGTACA BstBI 721 TTCGAA TT^CGAA 4352 TTCGAA Bsu36I 3876 CCTCAGG CC^T[ACGT]AGG 5184 CCTCAGG Cfr10I 967 GCCGGT [GA]^CCGG[CT] 5318 GCCGGT DraIII 118 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG 2168 CACTTAGTG Eco81I 3876 CCTCAGG CC^T[ACGT]AGG 5184 CCTCAGG EcoNI 1551 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4682 CCTGGAAGAGG FbaI 677 TGATCA T^GATCA 2934 TGATCA Hin1I 315 GACGCC G[GA]^CG[CT]C 409 GGCGCC MfeI 806 CAATTG C^AATTG 3587 CAATTG MluNI 2042 TGGCCA TGG^CCA 4553 TGGCCA MscI 2042 TGGCCA TGG^CCA 4553 TGGCCA MunI 806 CAATTG C^AATTG 3587 CAATTG NdeI 1443 CATATG CA^TATG 3065 CATATG NspV 721 TTCGAA TT^CGAA 4352 TTCGAA PmaCI 2531 CACGTG CAC^GTG 3814 CACGTG PmlI 2531 CACGTG CAC^GTG 3814 CACGTG PshAI 1810 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1892 GACCTAAGTC SapI 4785 GCTCTTC GCTCTTC(1/4) 5437 GCTCTTC SexAI 2547 ACCTGGT A^CC[AT]GGT 2737 ACCAGGT SfuI 721 TTCGAA TT^CGAA 4352 TTCGAA SphI 3762 GCATGC GCATG^C 3869 GCATGC SspBI 833 TGTACA T^GTACA 1884 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI Aor51HI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco47III EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII XmnI