##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AspI AviII BcgI
BfrI BsaBI BseAI BsiWI BspDI
BspEI BssHII Bst1107I BstZ17I ClaI
CpoI DrdI Eam1105I EcoRV FseI
FspI MamI MluI MroI NaeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SbfI
SfiI SgrAI SmaI Sse8387I Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 4186 GGTACC G^GTACC
Asp718I 4186 GGTACC G^GTACC
BbeI 409 GGCGCC GGCGC^C
BbvCI 4214 GCTGAGG CCTCAGC(-5/-2)
BglI 3873 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 180 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 180 GCTCAGC GC^T[ACGT]AGC
BsaWI 1682 ACCGGA [AT]^CCGG[AT]
BsmBI 4979 CGTCTC CGTCTC(1/5)
BsmI 2405 GAATGC GAATGC(1/-1)
BspHI 1340 TCATGA T^CATGA
BssSI 5508 CACGAG CACGAG(-5/-1)
BstAPI 2167 GCACTTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4694 GGTGACC G^GT[ACGT]ACC
BstPI 4694 GGTGACC G^GT[ACGT]ACC
CelII 180 GCTCAGC GC^T[ACGT]AGC
EagI 534 CGGCCG C^GGCCG
EclXI 534 CGGCCG C^GGCCG
Eco52I 534 CGGCCG C^GGCCG
EcoO65I 4694 GGTGACC G^GT[ACGT]ACC
EcoRI 1840 GAATTC G^AATTC
EcoT22I 3760 ATGCAT ATGCA^T
HpaI 3734 GTTAAC GTT^AAC
KasI 409 GGCGCC G^GCGCC
KpnI 4186 GGTACC GGTAC^C
NarI 409 GGCGCC GG^CGCC
NsiI 3760 ATGCAT ATGCA^T
RcaI 1340 TCATGA T^CATGA
ScaI 3494 AGTACT AGT^ACT
SfoI 409 GGCGCC GGC^GCC
SnaBI 4579 TACGTA TAC^GTA
SpeI 3117 ACTAGT A^CTAGT
SspI 3612 AATATT AAT^ATT
StuI 3770 AGGCCT AGG^CCT
SwaI 5690 ATTTAAAT ATTT^AAAT
XcmI 4699 CCAAGGAATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 315 GACGCC G[GA]^CG[CT]C
409 GGCGCC
AocI 3876 CCTCAGG CC^T[ACGT]AGG
5184 CCTCAGG
ApaI 148 GGGCCC GGGCC^C
1460 GGGCCC
AvaI 219 CTCGGG C^[CT]CG[GA]G
905 CTCGGG
AvrII 585 CCTAGG C^CTAGG
2765 CCTAGG
BalI 2042 TGGCCA TGG^CCA
4553 TGGCCA
BamHI 893 GGATCC G^GATCC
5078 GGATCC
BbrPI 2531 CACGTG CAC^GTG
3814 CACGTG
BciVI 621 GGATAC GTATCC(6/5)
3665 GTATCC
BclI 677 TGATCA T^GATCA
2934 TGATCA
BlnI 585 CCTAGG C^CTAGG
2765 CCTAGG
BsaHI 315 GACGCC G[GA]^CG[CT]C
409 GGCGCC
BsiEI 9 CGGTCG CG[GA][CT]^CG
534 CGGCCG
BsoBI 219 CTCGGG C^[CT]CG[GA]G
905 CTCGGG
Bsp1407I 833 TGTACA T^GTACA
1884 TGTACA
BsrFI 967 GCCGGT [GA]^CCGG[CT]
5318 GCCGGT
BsrGI 833 TGTACA T^GTACA
1884 TGTACA
BstBI 721 TTCGAA TT^CGAA
4352 TTCGAA
Bsu36I 3876 CCTCAGG CC^T[ACGT]AGG
5184 CCTCAGG
Cfr10I 967 GCCGGT [GA]^CCGG[CT]
5318 GCCGGT
DraIII 118 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2168 CACTTAGTG
Eco81I 3876 CCTCAGG CC^T[ACGT]AGG
5184 CCTCAGG
EcoNI 1551 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4682 CCTGGAAGAGG
FbaI 677 TGATCA T^GATCA
2934 TGATCA
Hin1I 315 GACGCC G[GA]^CG[CT]C
409 GGCGCC
MfeI 806 CAATTG C^AATTG
3587 CAATTG
MluNI 2042 TGGCCA TGG^CCA
4553 TGGCCA
MscI 2042 TGGCCA TGG^CCA
4553 TGGCCA
MunI 806 CAATTG C^AATTG
3587 CAATTG
NdeI 1443 CATATG CA^TATG
3065 CATATG
NspV 721 TTCGAA TT^CGAA
4352 TTCGAA
PmaCI 2531 CACGTG CAC^GTG
3814 CACGTG
PmlI 2531 CACGTG CAC^GTG
3814 CACGTG
PshAI 1810 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1892 GACCTAAGTC
SapI 4785 GCTCTTC GCTCTTC(1/4)
5437 GCTCTTC
SexAI 2547 ACCTGGT A^CC[AT]GGT
2737 ACCAGGT
SfuI 721 TTCGAA TT^CGAA
4352 TTCGAA
SphI 3762 GCATGC GCATG^C
3869 GCATGC
SspBI 833 TGTACA T^GTACA
1884 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApoI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BspMI BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII XmnI