##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI AscI Asp700I Asp718I
AspEI AvaI AviII BanI BbeI
BcgI BfrI BsaBI BsaWI BseAI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII BstBI BstUI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoRV FauI FseI FspI
HincII HindII HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 2665 GACGTC GACGT^C
Alw44I 472 GTGCAC G^TGCAC
Aor51HI 2597 AGCGCT AGC^GCT
ApaI 1882 GGGCCC GGGCC^C
ApaLI 472 GTGCAC G^TGCAC
AseI 1333 ATTAAT AT^TAAT
AsnI 1333 ATTAAT AT^TAAT
AvrII 902 CCTAGG C^CTAGG
BamHI 3551 GGATCC G^GATCC
BbrPI 424 CACGTG CAC^GTG
BciVI 3888 GGATAC GTATCC(6/5)
BlnI 902 CCTAGG C^CTAGG
BsaAI 424 CACGTG [CT]AC^GT[GA]
BsmBI 3809 CGTCTC CGTCTC(1/5)
BspHI 4179 TCATGA T^CATGA
BspMI 2921 ACCTGC ACCTGC(4/8)
Bst1107I 2917 GTATAC GTA^TAC
BstEII 1853 GGTCACC G^GT[ACGT]ACC
BstPI 1853 GGTCACC G^GT[ACGT]ACC
BstZ17I 2917 GTATAC GTA^TAC
DraIII 421 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2597 AGCGCT AGC^GCT
EcoO65I 1853 GGTCACC G^GT[ACGT]ACC
EcoT22I 2510 ATGCAT ATGCA^T
HindIII 2989 AAGCTT A^AGCTT
MspA1I 3796 CAGCTG C[AC]G^C[GT]G
NsiI 2510 ATGCAT ATGCA^T
PmaCI 424 CACGTG CAC^GTG
PmeI 3208 GTTTAAAC GTTT^AAAC
PmlI 424 CACGTG CAC^GTG
PshBI 1333 ATTAAT AT^TAAT
PvuII 3796 CAGCTG CAG^CTG
RcaI 4179 TCATGA T^CATGA
SpeI 1030 ACTAGT A^CTAGT
XbaI 1113 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AciI 1706 CCGC CCGC(-3/-1)
1569 GCGG
AcyI 2182 GACGCC G[GA]^CG[CT]C
2665 GACGTC
AlwNI 698 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2697 CAGCTACTG
AspHI 472 GTGCAC G[AT]GC[AT]^C
2170 GAGCAC
AspI 78 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3266 GACCAAGTC
BalI 1430 TGGCCA TGG^CCA
2318 TGGCCA
BbsI 367 GTCTTC GAAGAC(2/6)
2668 GTCTTC
BbvCI 546 GCTGAGG CCTCAGC(-5/-2)
1848 GCTGAGG
BclI 783 TGATCA T^GATCA
3914 TGATCA
BglI 2757 GCCTGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3085 GCCCAAAAGGC
BlpI 910 GCTGAGC GC^T[ACGT]AGC
2781 GCTGAGC
Bpu1102I 910 GCTGAGC GC^T[ACGT]AGC
2781 GCTGAGC
BpuAI 367 GTCTTC GAAGAC(2/6)
2668 GTCTTC
BsaHI 2182 GACGCC G[GA]^CG[CT]C
2665 GACGTC
BsgI 1225 CTGCAC GTGCAG(16/14)
1909 GTGCAG
BsiHKAI 472 GTGCAC G[AT]GC[AT]^C
2170 GAGCAC
BstAPI 1179 GCAATGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1708 GCACCCACTGC
BstXI 1421 CCAAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3795 CCAGCTGAGTGG
CelII 910 GCTGAGC GC^T[ACGT]AGC
2781 GCTGAGC
CfoI 2598 GCGC GCG^C
3392 GCGC
DsaI 1210 CCATGG C^C[GA][CT]GG
3742 CCATGG
EcoNI 1490 CCTTTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3605 CCTTGAGGAGG
EcoRI 3434 GAATTC G^AATTC
3700 GAATTC
FbaI 783 TGATCA T^GATCA
3914 TGATCA
HaeII 2597 AGCGCT [GA]GCGC^[CT]
3391 GGCGCT
HhaI 2598 GCGC GCG^C
3392 GCGC
Hin1I 2182 GACGCC G[GA]^CG[CT]C
2665 GACGTC
HinP1I 2598 GCGC G^CGC
3392 GCGC
MfeI 2339 CAATTG C^AATTG
2628 CAATTG
MluNI 1430 TGGCCA TGG^CCA
2318 TGGCCA
MscI 1430 TGGCCA TGG^CCA
2318 TGGCCA
MunI 2339 CAATTG C^AATTG
2628 CAATTG
NcoI 1210 CCATGG C^CATGG
3742 CCATGG
PflFI 78 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3266 GACCAAGTC
PflMI 231 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
420 CCACCACGTGG
SapI 459 GAAGAGC GCTCTTC(1/4)
1843 GAAGAGC
Tth111I 78 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3266 GACCAAGTC
Van91I 231 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
420 CCACCACGTGG
XcmI 423 CCACGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
732 CCAGCTACTTATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI AocI ApoI AvaII BanII
BbvI BcnI BfaI BglII BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII