##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AclI AflII AgeI AocI Aor51HI
AscI AseI AsnI AspI AviII
AvrII BalI BbrPI BclI BfrI
BlnI BsaBI BsiEI BsiWI BsmBI
BspDI BspLU11I BssHII Bst1107I BstZ17I
Bsu36I ClaI DraI EagI EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KspI MamI MfeI MluI MluNI
MscI MunI NaeI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI SacII SalI SbfI
SfiI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 750 GACGTC GACGT^C
Acc65I 3054 GGTACC G^GTACC
AccIII 379 TCCGGA T^CCGGA
AcsI 1143 GAATTC [GA]^AATT[CT]
AflIII 2080 ACACGT A^C[GA][CT]GT
AhdI 2475 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1143 GAATTC [GA]^AATT[CT]
Asp718I 3054 GGTACC G^GTACC
AspEI 2475 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 3010 GGCGCC GGCGC^C
BciVI 3658 GTATCC GTATCC(6/5)
BsaAI 3103 CACGTA [CT]AC^GT[GA]
BsaI 4008 GGTCTC GGTCTC(1/5)
BseAI 379 TCCGGA T^CCGGA
Bsp1407I 4301 TGTACA T^GTACA
BspEI 379 TCCGGA T^CCGGA
BsrBI 23 GAGCGG CCGCTC(-3/-3)
BsrFI 3028 ACCGGC [GA]^CCGG[CT]
BsrGI 4301 TGTACA T^GTACA
BssSI 3531 CACGAG CACGAG(-5/-1)
BstBI 2692 TTCGAA TT^CGAA
BstEII 1988 GGTGACC G^GT[ACGT]ACC
BstPI 1988 GGTGACC G^GT[ACGT]ACC
Cfr10I 3028 ACCGGC [GA]^CCGG[CT]
CpoI 2488 CGGACCG CG^G[AT]CCG
EaeI 704 TGGCCG [CT]^GGCC[GA]
Eam1105I 2475 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1988 GGTGACC G^GT[ACGT]ACC
EcoRI 1143 GAATTC G^AATTC
KasI 3010 GGCGCC G^GCGCC
KpnI 3054 GGTACC GGTAC^C
MroI 379 TCCGGA T^CCGGA
NarI 3010 GGCGCC GG^CGCC
NcoI 1522 CCATGG C^CATGG
NdeI 1411 CATATG CA^TATG
NspV 2692 TTCGAA TT^CGAA
PaeR7I 3245 CTCGAG C^TCGAG
RsrII 2488 CGGACCG CG^G[AT]CCG
ScaI 693 AGTACT AGT^ACT
SexAI 1429 ACCAGGT A^CC[AT]GGT
SfoI 3010 GGCGCC GGC^GCC
SfuI 2692 TTCGAA TT^CGAA
SmlI 3245 CTCGAG C^T[CT][GA]AG
SphI 1051 GCATGC GCATG^C
SspBI 4301 TGTACA T^GTACA
SspI 3690 AATATT AAT^ATT
XhoI 3245 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 2106 GTAGAC GT^[AC][GT]AC
2436 GTCTAC
AccII 80 CGCG CG^CG
670 CGCG
Alw44I 1074 GTGCAC G^TGCAC
3451 GTGCAC
ApaLI 1074 GTGCAC G^TGCAC
3451 GTGCAC
Asp700I 294 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
371 GAAGAAGTTC
BamHI 3570 GGATCC G^GATCC
3672 GGATCC
BcgI 598 GCAGGTCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1436 GCATACTCTTCG
BstUI 80 CGCG CG^CG
670 CGCG
DraIII 2574 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
3230 CACCCAGTG
DrdI 750 GACGTCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2679 GACAACAGCGTC
DsaI 1522 CCATGG C^C[GA][CT]GG
2485 CCACGG
HincII 1562 GTTGAC GT[CT]^[GA]AC
1736 GTTGAC
HindII 1562 GTTGAC GT[CT]^[GA]AC
1736 GTTGAC
MvnI 80 CGCG CG^CG
670 CGCG
PshAI 1686 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3815 GACCCATGTC
SacI 209 GAGCTC GAGCT^C
3248 GAGCTC
SapI 4094 GAAGAGC GCTCTTC(1/4)
2228 GCTCTTC
SmaI 113 CCCGGG CCC^GGG
1507 CCCGGG
Tsp45I 286 GTGAC ^GT[GC]AC
1989 GTGAC
XmaCI 113 CCCGGG C^CCGGG
1507 CCCGGG
XmaI 113 CCCGGG C^CCGGG
1507 CCCGGG
XmnI 294 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
371 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII