################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AgeI AocI AscI Asp700I Asp718I AvrII BbeI BbrPI BcgI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BsmBI Bsp1407I BspDI BsrGI BssHII Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DrdI EagI EclXI Eco52I Eco81I EcoNI EcoRI EcoRV FseI Hin1I HpaI KasI KpnI KspI MamI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 522 TCCGGA T^CCGGA AflII 2408 CTTAAG C^TTAAG Alw44I 1849 GTGCAC G^TGCAC Aor51HI 229 AGCGCT AGC^GCT ApaLI 1849 GTGCAC G^TGCAC AspI 2774 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 411 TGCGCA TGC^GCA BalI 2041 TGGCCA TGG^CCA BamHI 421 GGATCC G^GATCC BbvCI 259 GCTGAGG CCTCAGC(-5/-2) BclI 1909 TGATCA T^GATCA BfrI 2408 CTTAAG C^TTAAG BsaAI 3506 TACGTG [CT]AC^GT[GA] BseAI 522 TCCGGA T^CCGGA BspEI 522 TCCGGA T^CCGGA BsrBI 1836 GAGCGG CCGCTC(-3/-3) BsrFI 1049 GCCGGT [GA]^CCGG[CT] BssSI 2154 CACGAG CACGAG(-5/-1) BstEII 2873 GGTAACC G^GT[ACGT]ACC BstPI 2873 GGTAACC G^GT[ACGT]ACC Cfr10I 1049 GCCGGT [GA]^CCGG[CT] DraI 1118 TTTAAA TTT^AAA DraIII 1482 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2178 CCATGG C^C[GA][CT]GG Eco47III 229 AGCGCT AGC^GCT EcoO65I 2873 GGTAACC G^GT[ACGT]ACC FbaI 1909 TGATCA T^GATCA FspI 411 TGCGCA TGC^GCA HaeII 229 AGCGCT [GA]GCGC^[CT] MluNI 2041 TGGCCA TGG^CCA MroI 522 TCCGGA T^CCGGA MscI 2041 TGGCCA TGG^CCA NcoI 2178 CCATGG C^CATGG PaeR7I 1443 CTCGAG C^TCGAG PflFI 2774 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2323 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2295 AGGACCT [GA]G^G[AT]CC[CT] SacI 3931 GAGCTC GAGCT^C SapI 2480 GAAGAGC GCTCTTC(1/4) Tth111I 2774 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2323 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1443 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcsI 2845 AAATTC [GA]^AATT[CT] 3922 AAATTC AhdI 302 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2600 GACACAGAGTC ApaI 143 GGGCCC GGGCC^C 1779 GGGCCC ApoI 2845 AAATTC [GA]^AATT[CT] 3922 AAATTC AseI 3548 ATTAAT AT^TAAT 3653 ATTAAT AsnI 3548 ATTAAT AT^TAAT 3653 ATTAAT AspEI 302 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2600 GACACAGAGTC BbsI 1001 GAAGAC GAAGAC(2/6) 2639 GTCTTC BcnI 92 CCGGG CC^[GC]GG 152 CCGGG BpuAI 1001 GAAGAC GAAGAC(2/6) 2639 GTCTTC BsaI 428 GAGACC GGTCTC(1/5) 3668 GAGACC BsaWI 522 TCCGGA [AT]^CCGG[AT] 3675 ACCGGA BstAPI 1040 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1481 GCACAAGGTGC EaeI 246 TGGCCG [CT]^GGCC[GA] 2041 TGGCCA Eam1105I 302 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2600 GACACAGAGTC EcoT14I 1536 CCTTGG C^C[AT][AT]GG 2178 CCATGG EcoT22I 785 ATGCAT ATGCA^T 4173 ATGCAT HgaI 224 GACGC GACGC(5/10) 2977 GACGC MaeII 1270 ACGT A^CGT 3507 ACGT MfeI 4030 CAATTG C^AATTG 4241 CAATTG MunI 4030 CAATTG C^AATTG 4241 CAATTG NciI 92 CCGGG CC^[GC]GG 152 CCGGG NsiI 785 ATGCAT ATGCA^T 4173 ATGCAT PshBI 3548 ATTAAT AT^TAAT 3653 ATTAAT PstI 2279 CTGCAG CTGCA^G 4268 CTGCAG PvuII 66 CAGCTG CAG^CTG 3009 CAGCTG ScaI 517 AGTACT AGT^ACT 772 AGTACT SphI 787 GCATGC GCATG^C 1690 GCATGC SspI 1662 AATATT AAT^ATT 3512 AATATT StyI 1536 CCTTGG C^C[AT][AT]GG 2178 CCATGG XbaI 1078 TCTAGA T^CTAGA 4314 TCTAGA XcmI 1006 CCATAATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2568 CCAAAGAATACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BciVI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII