##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AgeI AocI AscI Asp700I Asp718I
AvrII BbeI BbrPI BcgI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsiEI BsiWI BsmBI Bsp1407I
BspDI BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DrdI EagI EclXI Eco52I
Eco81I EcoNI EcoRI EcoRV FseI
Hin1I HpaI KasI KpnI KspI
MamI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 522 TCCGGA T^CCGGA
AflII 2408 CTTAAG C^TTAAG
Alw44I 1849 GTGCAC G^TGCAC
Aor51HI 229 AGCGCT AGC^GCT
ApaLI 1849 GTGCAC G^TGCAC
AspI 2774 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 411 TGCGCA TGC^GCA
BalI 2041 TGGCCA TGG^CCA
BamHI 421 GGATCC G^GATCC
BbvCI 259 GCTGAGG CCTCAGC(-5/-2)
BclI 1909 TGATCA T^GATCA
BfrI 2408 CTTAAG C^TTAAG
BsaAI 3506 TACGTG [CT]AC^GT[GA]
BseAI 522 TCCGGA T^CCGGA
BspEI 522 TCCGGA T^CCGGA
BsrBI 1836 GAGCGG CCGCTC(-3/-3)
BsrFI 1049 GCCGGT [GA]^CCGG[CT]
BssSI 2154 CACGAG CACGAG(-5/-1)
BstEII 2873 GGTAACC G^GT[ACGT]ACC
BstPI 2873 GGTAACC G^GT[ACGT]ACC
Cfr10I 1049 GCCGGT [GA]^CCGG[CT]
DraI 1118 TTTAAA TTT^AAA
DraIII 1482 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2178 CCATGG C^C[GA][CT]GG
Eco47III 229 AGCGCT AGC^GCT
EcoO65I 2873 GGTAACC G^GT[ACGT]ACC
FbaI 1909 TGATCA T^GATCA
FspI 411 TGCGCA TGC^GCA
HaeII 229 AGCGCT [GA]GCGC^[CT]
MluNI 2041 TGGCCA TGG^CCA
MroI 522 TCCGGA T^CCGGA
MscI 2041 TGGCCA TGG^CCA
NcoI 2178 CCATGG C^CATGG
PaeR7I 1443 CTCGAG C^TCGAG
PflFI 2774 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2323 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2295 AGGACCT [GA]G^G[AT]CC[CT]
SacI 3931 GAGCTC GAGCT^C
SapI 2480 GAAGAGC GCTCTTC(1/4)
Tth111I 2774 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2323 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1443 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 2845 AAATTC [GA]^AATT[CT]
3922 AAATTC
AhdI 302 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2600 GACACAGAGTC
ApaI 143 GGGCCC GGGCC^C
1779 GGGCCC
ApoI 2845 AAATTC [GA]^AATT[CT]
3922 AAATTC
AseI 3548 ATTAAT AT^TAAT
3653 ATTAAT
AsnI 3548 ATTAAT AT^TAAT
3653 ATTAAT
AspEI 302 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2600 GACACAGAGTC
BbsI 1001 GAAGAC GAAGAC(2/6)
2639 GTCTTC
BcnI 92 CCGGG CC^[GC]GG
152 CCGGG
BpuAI 1001 GAAGAC GAAGAC(2/6)
2639 GTCTTC
BsaI 428 GAGACC GGTCTC(1/5)
3668 GAGACC
BsaWI 522 TCCGGA [AT]^CCGG[AT]
3675 ACCGGA
BstAPI 1040 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1481 GCACAAGGTGC
EaeI 246 TGGCCG [CT]^GGCC[GA]
2041 TGGCCA
Eam1105I 302 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2600 GACACAGAGTC
EcoT14I 1536 CCTTGG C^C[AT][AT]GG
2178 CCATGG
EcoT22I 785 ATGCAT ATGCA^T
4173 ATGCAT
HgaI 224 GACGC GACGC(5/10)
2977 GACGC
MaeII 1270 ACGT A^CGT
3507 ACGT
MfeI 4030 CAATTG C^AATTG
4241 CAATTG
MunI 4030 CAATTG C^AATTG
4241 CAATTG
NciI 92 CCGGG CC^[GC]GG
152 CCGGG
NsiI 785 ATGCAT ATGCA^T
4173 ATGCAT
PshBI 3548 ATTAAT AT^TAAT
3653 ATTAAT
PstI 2279 CTGCAG CTGCA^G
4268 CTGCAG
PvuII 66 CAGCTG CAG^CTG
3009 CAGCTG
ScaI 517 AGTACT AGT^ACT
772 AGTACT
SphI 787 GCATGC GCATG^C
1690 GCATGC
SspI 1662 AATATT AAT^ATT
3512 AATATT
StyI 1536 CCTTGG C^C[AT][AT]GG
2178 CCATGG
XbaI 1078 TCTAGA T^CTAGA
4314 TCTAGA
XcmI 1006 CCATAATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2568 CCAAAGAATACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BciVI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII