##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AgeI        AocI        AscI        Asp700I     Asp718I     
AvrII       BbeI        BbrPI       BcgI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BsrGI       BssHII      Bst1107I    BstBI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DrdI        EagI        EclXI       Eco52I      
Eco81I      EcoNI       EcoRI       EcoRV       FseI        
Hin1I       HpaI        KasI        KpnI        KspI        
MamI        MluI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       StuI        SwaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccIII        522  TCCGGA               T^CCGGA
AflII        2408  CTTAAG               C^TTAAG
Alw44I       1849  GTGCAC               G^TGCAC
Aor51HI       229  AGCGCT               AGC^GCT
ApaLI        1849  GTGCAC               G^TGCAC
AspI         2774  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         411  TGCGCA               TGC^GCA
BalI         2041  TGGCCA               TGG^CCA
BamHI         421  GGATCC               G^GATCC
BbvCI         259  GCTGAGG              CCTCAGC(-5/-2)
BclI         1909  TGATCA               T^GATCA
BfrI         2408  CTTAAG               C^TTAAG
BsaAI        3506  TACGTG               [CT]AC^GT[GA]
BseAI         522  TCCGGA               T^CCGGA
BspEI         522  TCCGGA               T^CCGGA
BsrBI        1836  GAGCGG               CCGCTC(-3/-3)
BsrFI        1049  GCCGGT               [GA]^CCGG[CT]
BssSI        2154  CACGAG               CACGAG(-5/-1)
BstEII       2873  GGTAACC              G^GT[ACGT]ACC
BstPI        2873  GGTAACC              G^GT[ACGT]ACC
Cfr10I       1049  GCCGGT               [GA]^CCGG[CT]
DraI         1118  TTTAAA               TTT^AAA
DraIII       1482  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         2178  CCATGG               C^C[GA][CT]GG
Eco47III      229  AGCGCT               AGC^GCT
EcoO65I      2873  GGTAACC              G^GT[ACGT]ACC
FbaI         1909  TGATCA               T^GATCA
FspI          411  TGCGCA               TGC^GCA
HaeII         229  AGCGCT               [GA]GCGC^[CT]
MluNI        2041  TGGCCA               TGG^CCA
MroI          522  TCCGGA               T^CCGGA
MscI         2041  TGGCCA               TGG^CCA
NcoI         2178  CCATGG               C^CATGG
PaeR7I       1443  CTCGAG               C^TCGAG
PflFI        2774  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2323  CCAGCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2295  AGGACCT              [GA]G^G[AT]CC[CT]
SacI         3931  GAGCTC               GAGCT^C
SapI         2480  GAAGAGC              GCTCTTC(1/4)
Tth111I      2774  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2323  CCAGCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1443  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcsI         2845  AAATTC               [GA]^AATT[CT]
             3922  AAATTC              
AhdI          302  GACGAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2600  GACACAGAGTC         
ApaI          143  GGGCCC               GGGCC^C
             1779  GGGCCC              
ApoI         2845  AAATTC               [GA]^AATT[CT]
             3922  AAATTC              
AseI         3548  ATTAAT               AT^TAAT
             3653  ATTAAT              
AsnI         3548  ATTAAT               AT^TAAT
             3653  ATTAAT              
AspEI         302  GACGAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2600  GACACAGAGTC         
BbsI         1001  GAAGAC               GAAGAC(2/6)
             2639  GTCTTC              
BcnI           92  CCGGG                CC^[GC]GG
              152  CCGGG               
BpuAI        1001  GAAGAC               GAAGAC(2/6)
             2639  GTCTTC              
BsaI          428  GAGACC               GGTCTC(1/5)
             3668  GAGACC              
BsaWI         522  TCCGGA               [AT]^CCGG[AT]
             3675  ACCGGA              
BstAPI       1040  GCAGCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1481  GCACAAGGTGC         
EaeI          246  TGGCCG               [CT]^GGCC[GA]
             2041  TGGCCA              
Eam1105I      302  GACGAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2600  GACACAGAGTC         
EcoT14I      1536  CCTTGG               C^C[AT][AT]GG
             2178  CCATGG              
EcoT22I       785  ATGCAT               ATGCA^T
             4173  ATGCAT              
HgaI          224  GACGC                GACGC(5/10)
             2977  GACGC               
MaeII        1270  ACGT                 A^CGT
             3507  ACGT                
MfeI         4030  CAATTG               C^AATTG
             4241  CAATTG              
MunI         4030  CAATTG               C^AATTG
             4241  CAATTG              
NciI           92  CCGGG                CC^[GC]GG
              152  CCGGG               
NsiI          785  ATGCAT               ATGCA^T
             4173  ATGCAT              
PshBI        3548  ATTAAT               AT^TAAT
             3653  ATTAAT              
PstI         2279  CTGCAG               CTGCA^G
             4268  CTGCAG              
PvuII          66  CAGCTG               CAG^CTG
             3009  CAGCTG              
ScaI          517  AGTACT               AGT^ACT
              772  AGTACT              
SphI          787  GCATGC               GCATG^C
             1690  GCATGC              
SspI         1662  AATATT               AAT^ATT
             3512  AATATT              
StyI         1536  CCTTGG               C^C[AT][AT]GG
             2178  CCATGG              
XbaI         1078  TCTAGA               T^CTAGA
             4314  TCTAGA              
XcmI         1006  CCATAATGAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2568  CCAAAGAATACTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BciVI       BfaI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspHI       BspLU11I    
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII