################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AccIII AcyI AgeI AlwNI Aor51HI ApaI AscI Asp700I AviII AvrII BanII BbeI BbrPI BbvCI BlnI BlpI Bpu1102I BsaHI BseAI BsiWI BspEI BsrBI BssHII Bst1107I BstZ17I CelII CpoI DraIII EagI EclXI Eco47III Eco52I EcoRI FseI FspI HaeII HgaI Hin1I HpaI KasI KspI MroI NarI NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SgrAI SmaI Sse8387I StuI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AclI 4646 AACGTT AA^CGTT AflII 1685 CTTAAG C^TTAAG Alw44I 4147 GTGCAC G^TGCAC ApaLI 4147 GTGCAC G^TGCAC AseI 11 ATTAAT AT^TAAT AsnI 11 ATTAAT AT^TAAT AvaI 57 CCCGAG C^[CT]CG[GA]G BciVI 4370 GTATCC GTATCC(6/5) BclI 1290 TGATCA T^GATCA BfrI 1685 CTTAAG C^TTAAG BglI 2326 GCCAGCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 2090 TACGTA [CT]AC^GT[GA] BsaBI 2553 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1624 ACCGGA [AT]^CCGG[AT] BsiEI 4273 CGGTCG CG[GA][CT]^CG BsmBI 3923 GAGACG CGTCTC(1/5) BsoBI 57 CCCGAG C^[CT]CG[GA]G BspDI 2896 ATCGAT AT^CGAT BspLU11I 3648 ACATGT A^CATGT BspMI 3552 ACCTGC ACCTGC(4/8) BsrFI 3968 GCCGGC [GA]^CCGG[CT] BssSI 925 CTCGTG CACGAG(-5/-1) BstBI 2981 TTCGAA TT^CGAA BstEII 2797 GGTTACC G^GT[ACGT]ACC BstPI 2797 GGTTACC G^GT[ACGT]ACC CfoI 3895 GCGC GCG^C Cfr10I 3968 GCCGGC [GA]^CCGG[CT] ClaI 2896 ATCGAT AT^CGAT EcoNI 1390 CCTGAGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2797 GGTTACC G^GT[ACGT]ACC EcoRV 3778 GATATC GAT^ATC FbaI 1290 TGATCA T^GATCA HhaI 3895 GCGC GCG^C HinP1I 3895 GCGC G^CGC HincII 1855 GTTGAC GT[CT]^[GA]AC HindII 1855 GTTGAC GT[CT]^[GA]AC HindIII 1116 AAGCTT A^AGCTT MamI 2553 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 559 ACGCGT A^CGCGT NaeI 3968 GCCGGC GCC^GGC NgoMIV 3968 GCCGGC G^CCGGC NspV 2981 TTCGAA TT^CGAA PflMI 1870 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 11 ATTAAT AT^TAAT Psp1406I 4646 AACGTT AA^CGTT SapI 258 GCTCTTC GCTCTTC(1/4) SfuI 2981 TTCGAA TT^CGAA SnaBI 2090 TACGTA TAC^GTA SpeI 737 ACTAGT A^CTAGT Van91I 1870 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 874 GGTACC G^GTACC 1470 GGTACC AhdI 3745 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3905 GACTGTGTGTC Asp718I 874 GGTACC G^GTACC 1470 GGTACC AspEI 3745 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3905 GACTGTGTGTC AspI 3833 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3925 GACGTTGTC BamHI 3002 GGATCC G^GATCC 3797 GGATCC BanI 874 GGTACC G^G[CT][GA]CC 1470 GGTACC BbsI 2005 GAAGAC GAAGAC(2/6) 3886 GAAGAC BcgI 84 CGATCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1512 CGAAGTAGGTGC BcnI 3233 CCCGG CC^[GC]GG 4311 CCCGG BglII 2380 AGATCT A^GATCT 2517 AGATCT BpuAI 2005 GAAGAC GAAGAC(2/6) 3886 GAAGAC BsaI 2818 GAGACC GGTCTC(1/5) 3838 GGTCTC BsgI 3554 CTGCAC GTGCAG(16/14) 4067 CTGCAC BsmI 4599 GAATGC GAATGC(1/-1) 829 GCATTC BspHI 4392 TCATGA T^CATGA 4496 TCATGA DraII 268 AGGACCC [GA]G^G[ACGT]CC[CT] 3831 AGGACCT DrdI 1360 GACTGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3935 GACTTTAAGGTC Eam1105I 3745 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3905 GACTGTGTGTC EcoO109I 268 AGGACCC [GA]G^G[ACGT]CC[CT] 3831 AGGACCT EcoT22I 2637 ATGCAT ATGCA^T 3699 ATGCAT KpnI 874 GGTACC GGTAC^C 1470 GGTACC NciI 3233 CCCGG CC^[GC]GG 4311 CCCGG NsiI 2637 ATGCAT ATGCA^T 3699 ATGCAT PflFI 3833 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3925 GACGTTGTC PpuMI 268 AGGACCC [GA]G^G[AT]CC[CT] 3831 AGGACCT RcaI 4392 TCATGA T^CATGA 4496 TCATGA SexAI 1850 ACCAGGT A^CC[AT]GGT 3834 ACCTGGT SspI 2491 AATATT AAT^ATT 4585 AATATT Tth111I 3833 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3925 GACGTTGTC XcmI 941 CCAGAGAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3607 CCAACAGAAGGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AocI ApoI AspHI AvaII BalI BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DsaI EaeI EarI Eco81I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII