##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AcyI AgeI AlwNI
Aor51HI ApaI AscI Asp700I AviII
AvrII BanII BbeI BbrPI BbvCI
BlnI BlpI Bpu1102I BsaHI BseAI
BsiWI BspEI BsrBI BssHII Bst1107I
BstZ17I CelII CpoI DraIII EagI
EclXI Eco47III Eco52I EcoRI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KspI MroI NarI NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SgrAI SmaI Sse8387I
StuI SwaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AclI 4646 AACGTT AA^CGTT
AflII 1685 CTTAAG C^TTAAG
Alw44I 4147 GTGCAC G^TGCAC
ApaLI 4147 GTGCAC G^TGCAC
AseI 11 ATTAAT AT^TAAT
AsnI 11 ATTAAT AT^TAAT
AvaI 57 CCCGAG C^[CT]CG[GA]G
BciVI 4370 GTATCC GTATCC(6/5)
BclI 1290 TGATCA T^GATCA
BfrI 1685 CTTAAG C^TTAAG
BglI 2326 GCCAGCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2090 TACGTA [CT]AC^GT[GA]
BsaBI 2553 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1624 ACCGGA [AT]^CCGG[AT]
BsiEI 4273 CGGTCG CG[GA][CT]^CG
BsmBI 3923 GAGACG CGTCTC(1/5)
BsoBI 57 CCCGAG C^[CT]CG[GA]G
BspDI 2896 ATCGAT AT^CGAT
BspLU11I 3648 ACATGT A^CATGT
BspMI 3552 ACCTGC ACCTGC(4/8)
BsrFI 3968 GCCGGC [GA]^CCGG[CT]
BssSI 925 CTCGTG CACGAG(-5/-1)
BstBI 2981 TTCGAA TT^CGAA
BstEII 2797 GGTTACC G^GT[ACGT]ACC
BstPI 2797 GGTTACC G^GT[ACGT]ACC
CfoI 3895 GCGC GCG^C
Cfr10I 3968 GCCGGC [GA]^CCGG[CT]
ClaI 2896 ATCGAT AT^CGAT
EcoNI 1390 CCTGAGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2797 GGTTACC G^GT[ACGT]ACC
EcoRV 3778 GATATC GAT^ATC
FbaI 1290 TGATCA T^GATCA
HhaI 3895 GCGC GCG^C
HinP1I 3895 GCGC G^CGC
HincII 1855 GTTGAC GT[CT]^[GA]AC
HindII 1855 GTTGAC GT[CT]^[GA]AC
HindIII 1116 AAGCTT A^AGCTT
MamI 2553 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 559 ACGCGT A^CGCGT
NaeI 3968 GCCGGC GCC^GGC
NgoMIV 3968 GCCGGC G^CCGGC
NspV 2981 TTCGAA TT^CGAA
PflMI 1870 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 11 ATTAAT AT^TAAT
Psp1406I 4646 AACGTT AA^CGTT
SapI 258 GCTCTTC GCTCTTC(1/4)
SfuI 2981 TTCGAA TT^CGAA
SnaBI 2090 TACGTA TAC^GTA
SpeI 737 ACTAGT A^CTAGT
Van91I 1870 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 874 GGTACC G^GTACC
1470 GGTACC
AhdI 3745 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3905 GACTGTGTGTC
Asp718I 874 GGTACC G^GTACC
1470 GGTACC
AspEI 3745 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3905 GACTGTGTGTC
AspI 3833 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3925 GACGTTGTC
BamHI 3002 GGATCC G^GATCC
3797 GGATCC
BanI 874 GGTACC G^G[CT][GA]CC
1470 GGTACC
BbsI 2005 GAAGAC GAAGAC(2/6)
3886 GAAGAC
BcgI 84 CGATCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1512 CGAAGTAGGTGC
BcnI 3233 CCCGG CC^[GC]GG
4311 CCCGG
BglII 2380 AGATCT A^GATCT
2517 AGATCT
BpuAI 2005 GAAGAC GAAGAC(2/6)
3886 GAAGAC
BsaI 2818 GAGACC GGTCTC(1/5)
3838 GGTCTC
BsgI 3554 CTGCAC GTGCAG(16/14)
4067 CTGCAC
BsmI 4599 GAATGC GAATGC(1/-1)
829 GCATTC
BspHI 4392 TCATGA T^CATGA
4496 TCATGA
DraII 268 AGGACCC [GA]G^G[ACGT]CC[CT]
3831 AGGACCT
DrdI 1360 GACTGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3935 GACTTTAAGGTC
Eam1105I 3745 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3905 GACTGTGTGTC
EcoO109I 268 AGGACCC [GA]G^G[ACGT]CC[CT]
3831 AGGACCT
EcoT22I 2637 ATGCAT ATGCA^T
3699 ATGCAT
KpnI 874 GGTACC GGTAC^C
1470 GGTACC
NciI 3233 CCCGG CC^[GC]GG
4311 CCCGG
NsiI 2637 ATGCAT ATGCA^T
3699 ATGCAT
PflFI 3833 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3925 GACGTTGTC
PpuMI 268 AGGACCC [GA]G^G[AT]CC[CT]
3831 AGGACCT
RcaI 4392 TCATGA T^CATGA
4496 TCATGA
SexAI 1850 ACCAGGT A^CC[AT]GGT
3834 ACCTGGT
SspI 2491 AATATT AAT^ATT
4585 AATATT
Tth111I 3833 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3925 GACGTTGTC
XcmI 941 CCAGAGAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3607 CCAACAGAAGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AocI ApoI
AspHI AvaII BalI BbvI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
Eco81I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MvnI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII