##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AclI AflII AocI ApaI
AscI AspI AvaI AviII BalI
BcgI BciVI BfrI BglI BsaBI
BsiEI BsiWI BsmBI BsoBI BsrBI
BssHII Bst1107I BstEII BstPI BstZ17I
Bsu36I CpoI DraIII DrdI EaeI
EagI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FseI FspI
HgaI KspI MamI MfeI MluI
MluNI MscI MunI NaeI NdeI
NgoMIV NotI NruI PaeR7I PflFI
PmeI Psp1406I PstI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SgrAI SmaI SnaBI Sse8387I Tth111I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 1293 GTAGAC GT^[AC][GT]AC
AccIII 2533 TCCGGA T^CCGGA
AcyI 39 GGCGCC G[GA]^CG[CT]C
AhdI 3193 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4275 GTGCAC G^TGCAC
Aor51HI 32 AGCGCT AGC^GCT
ApaLI 4275 GTGCAC G^TGCAC
AspEI 3193 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 5487 CCTAGG C^CTAGG
BbeI 39 GGCGCC GGCGC^C
BbrPI 5383 CACGTG CAC^GTG
BclI 352 TGATCA T^GATCA
BcnI 4869 CCGGG CC^[GC]GG
BlnI 5487 CCTAGG C^CTAGG
BsaAI 5383 CACGTG [CT]AC^GT[GA]
BsaHI 39 GGCGCC G[GA]^CG[CT]C
BsaI 5406 GAGACC GGTCTC(1/5)
BseAI 2533 TCCGGA T^CCGGA
BseRI 5500 GAGGAG GAGGAG(10/8)
BspDI 1407 ATCGAT AT^CGAT
BspEI 2533 TCCGGA T^CCGGA
BspHI 1787 TCATGA T^CATGA
BspLU11I 2133 ACATGT A^CATGT
BspMI 5361 ACCTGC ACCTGC(4/8)
BssSI 1332 CTCGTG CACGAG(-5/-1)
BstBI 1744 TTCGAA TT^CGAA
ClaI 1407 ATCGAT AT^CGAT
DraII 25 GGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 3193 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 32 AGCGCT AGC^GCT
EcoO109I 25 GGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 352 TGATCA T^GATCA
Hin1I 39 GGCGCC G[GA]^CG[CT]C
HincII 46 GTTAAC GT[CT]^[GA]AC
HindII 46 GTTAAC GT[CT]^[GA]AC
HpaI 46 GTTAAC GTT^AAC
KasI 39 GGCGCC G^GCGCC
MaeII 5384 ACGT A^CGT
MroI 2533 TCCGGA T^CCGGA
NarI 39 GGCGCC GG^CGCC
NciI 4869 CCGGG CC^[GC]GG
NheI 2987 GCTAGC G^CTAGC
NspV 1744 TTCGAA TT^CGAA
PacI 2118 TTAATTAA TTAAT^TAA
PmaCI 5383 CACGTG CAC^GTG
PmlI 5383 CACGTG CAC^GTG
PpuMI 25 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 2874 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1787 TCATGA T^CATGA
SapI 2255 GAAGAGC GCTCTTC(1/4)
ScaI 4741 AGTACT AGT^ACT
SfoI 39 GGCGCC GGC^GCC
SfuI 1744 TTCGAA TT^CGAA
SpeI 3507 ACTAGT A^CTAGT
SphI 4026 GCATGC GCATG^C
SwaI 3976 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 65 CGCG CG^CG
1585 CGCG
AflIII 2133 ACATGT A^C[GA][CT]GT
5384 ACGTGT
AgeI 1324 ACCGGT A^CCGGT
4071 ACCGGT
BamHI 4677 GGATCC G^GATCC
4812 GGATCC
BbvCI 1714 CCTCAGC CCTCAGC(-5/-2)
4563 CCTCAGC
BpmI 621 CTCCAG CTGGAG(16/14)
5291 CTGGAG
Bsp1407I 525 TGTACA T^GTACA
4997 TGTACA
BsrGI 525 TGTACA T^GTACA
4997 TGTACA
BstAPI 1769 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5096 GCATACTATGC
BstUI 65 CGCG CG^CG
1585 CGCG
DsaI 73 CCATGG C^C[GA][CT]GG
5401 CCATGG
FauI 66 GCGGG CCCGC(4/6)
127 GCGGG
HaeIII 153 GGCC GG^CC
303 GGCC
MvnI 65 CGCG CG^CG
1585 CGCG
NcoI 73 CCATGG C^CATGG
5401 CCATGG
PflMI 1694 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5243 CCACAATCTGG
PinAI 1324 ACCGGT A^CCGGT
4071 ACCGGT
SacI 1704 GAGCTC GAGCT^C
2384 GAGCTC
SmlI 2617 CTCAAG C^T[CT][GA]AG
2883 CTTGAG
SspBI 525 TGTACA T^GTACA
4997 TGTACA
StuI 152 AGGCCT AGG^CCT
302 AGGCCT
Van91I 1694 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5243 CCACAATCTGG
XbaI 3722 TCTAGA T^CTAGA
5496 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I Asp718I AspHI AvaII BanI
BanII BbsI BbvI BfaI BglII
BlpI BmyI Bpu1102I BpuAI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI