################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AclI AflII AocI ApaI AscI AspI AvaI AviII BalI BcgI BciVI BfrI BglI BsaBI BsiEI BsiWI BsmBI BsoBI BsrBI BssHII Bst1107I BstEII BstPI BstZ17I Bsu36I CpoI DraIII DrdI EaeI EagI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FseI FspI HgaI KspI MamI MfeI MluI MluNI MscI MunI NaeI NdeI NgoMIV NotI NruI PaeR7I PflFI PmeI Psp1406I PstI PvuI RsrII SacII SalI SbfI SexAI SfiI SgrAI SmaI SnaBI Sse8387I Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 1293 GTAGAC GT^[AC][GT]AC AccIII 2533 TCCGGA T^CCGGA AcyI 39 GGCGCC G[GA]^CG[CT]C AhdI 3193 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4275 GTGCAC G^TGCAC Aor51HI 32 AGCGCT AGC^GCT ApaLI 4275 GTGCAC G^TGCAC AspEI 3193 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 5487 CCTAGG C^CTAGG BbeI 39 GGCGCC GGCGC^C BbrPI 5383 CACGTG CAC^GTG BclI 352 TGATCA T^GATCA BcnI 4869 CCGGG CC^[GC]GG BlnI 5487 CCTAGG C^CTAGG BsaAI 5383 CACGTG [CT]AC^GT[GA] BsaHI 39 GGCGCC G[GA]^CG[CT]C BsaI 5406 GAGACC GGTCTC(1/5) BseAI 2533 TCCGGA T^CCGGA BseRI 5500 GAGGAG GAGGAG(10/8) BspDI 1407 ATCGAT AT^CGAT BspEI 2533 TCCGGA T^CCGGA BspHI 1787 TCATGA T^CATGA BspLU11I 2133 ACATGT A^CATGT BspMI 5361 ACCTGC ACCTGC(4/8) BssSI 1332 CTCGTG CACGAG(-5/-1) BstBI 1744 TTCGAA TT^CGAA ClaI 1407 ATCGAT AT^CGAT DraII 25 GGGACCC [GA]G^G[ACGT]CC[CT] Eam1105I 3193 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 32 AGCGCT AGC^GCT EcoO109I 25 GGGACCC [GA]G^G[ACGT]CC[CT] FbaI 352 TGATCA T^GATCA Hin1I 39 GGCGCC G[GA]^CG[CT]C HincII 46 GTTAAC GT[CT]^[GA]AC HindII 46 GTTAAC GT[CT]^[GA]AC HpaI 46 GTTAAC GTT^AAC KasI 39 GGCGCC G^GCGCC MaeII 5384 ACGT A^CGT MroI 2533 TCCGGA T^CCGGA NarI 39 GGCGCC GG^CGCC NciI 4869 CCGGG CC^[GC]GG NheI 2987 GCTAGC G^CTAGC NspV 1744 TTCGAA TT^CGAA PacI 2118 TTAATTAA TTAAT^TAA PmaCI 5383 CACGTG CAC^GTG PmlI 5383 CACGTG CAC^GTG PpuMI 25 GGGACCC [GA]G^G[AT]CC[CT] PshAI 2874 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1787 TCATGA T^CATGA SapI 2255 GAAGAGC GCTCTTC(1/4) ScaI 4741 AGTACT AGT^ACT SfoI 39 GGCGCC GGC^GCC SfuI 1744 TTCGAA TT^CGAA SpeI 3507 ACTAGT A^CTAGT SphI 4026 GCATGC GCATG^C SwaI 3976 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 65 CGCG CG^CG 1585 CGCG AflIII 2133 ACATGT A^C[GA][CT]GT 5384 ACGTGT AgeI 1324 ACCGGT A^CCGGT 4071 ACCGGT BamHI 4677 GGATCC G^GATCC 4812 GGATCC BbvCI 1714 CCTCAGC CCTCAGC(-5/-2) 4563 CCTCAGC BpmI 621 CTCCAG CTGGAG(16/14) 5291 CTGGAG Bsp1407I 525 TGTACA T^GTACA 4997 TGTACA BsrGI 525 TGTACA T^GTACA 4997 TGTACA BstAPI 1769 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5096 GCATACTATGC BstUI 65 CGCG CG^CG 1585 CGCG DsaI 73 CCATGG C^C[GA][CT]GG 5401 CCATGG FauI 66 GCGGG CCCGC(4/6) 127 GCGGG HaeIII 153 GGCC GG^CC 303 GGCC MvnI 65 CGCG CG^CG 1585 CGCG NcoI 73 CCATGG C^CATGG 5401 CCATGG PflMI 1694 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5243 CCACAATCTGG PinAI 1324 ACCGGT A^CCGGT 4071 ACCGGT SacI 1704 GAGCTC GAGCT^C 2384 GAGCTC SmlI 2617 CTCAAG C^T[CT][GA]AG 2883 CTTGAG SspBI 525 TGTACA T^GTACA 4997 TGTACA StuI 152 AGGCCT AGG^CCT 302 AGGCCT Van91I 1694 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5243 CCACAATCTGG XbaI 3722 TCTAGA T^CTAGA 5496 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### Acc65I AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I Asp718I AspHI AvaII BanI BanII BbsI BbvI BfaI BglII BlpI BmyI Bpu1102I BpuAI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI