################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvrII BalI BbeI BbvCI BciVI BfrI BlnI BseAI BsiEI BsiWI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CpoI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT14I EcoT22I FauI FseI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I StyI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 1580 GACGTC GACGT^C AccI 2522 GTCTAC GT^[AC][GT]AC AocI 2127 CCTCAGG CC^T[ACGT]AGG Asp700I 2453 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 193 TGCGCA TGC^GCA BanI 1595 GGCACC G^G[CT][GA]CC BbrPI 855 CACGTG CAC^GTG BcgI 3082 CGAGGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 67 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1322 AGATCT A^GATCT BlpI 155 GCTGAGC GC^T[ACGT]AGC Bpu1102I 155 GCTGAGC GC^T[ACGT]AGC BsaAI 855 CACGTG [CT]AC^GT[GA] BsaBI 1014 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2015 TCCGGT [AT]^CCGG[AT] BsmBI 167 GAGACG CGTCTC(1/5) Bsp1407I 3187 TGTACA T^GTACA BspDI 1153 ATCGAT AT^CGAT BsrFI 67 GCCGGC [GA]^CCGG[CT] BsrGI 3187 TGTACA T^GTACA Bsu36I 2127 CCTCAGG CC^T[ACGT]AGG CelII 155 GCTGAGC GC^T[ACGT]AGC Cfr10I 67 GCCGGC [GA]^CCGG[CT] ClaI 1153 ATCGAT AT^CGAT DraI 3329 TTTAAA TTT^AAA DrdI 1752 GACATTAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2127 CCTCAGG CC^T[ACGT]AGG EcoNI 3257 CCTGTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 193 TGCGCA TGC^GCA HaeII 1443 GGCGCT [GA]GCGC^[CT] HindIII 1695 AAGCTT A^AGCTT HpaI 992 GTTAAC GTT^AAC MamI 1014 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 67 GCCGGC GCC^GGC NgoMIV 67 GCCGGC G^CCGGC PflMI 437 CCATTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 855 CACGTG CAC^GTG PmlI 855 CACGTG CAC^GTG PstI 2032 CTGCAG CTGCA^G PvuII 2029 CAGCTG CAG^CTG SacI 2364 GAGCTC GAGCT^C SapI 3092 GCTCTTC GCTCTTC(1/4) ScaI 1965 AGTACT AGT^ACT SexAI 1632 ACCAGGT A^CC[AT]GGT SmlI 1879 CTCAAG C^T[CT][GA]AG SpeI 2611 ACTAGT A^CTAGT SphI 200 GCATGC GCATG^C SspBI 3187 TGTACA T^GTACA SspI 1866 AATATT AAT^ATT Van91I 437 CCATTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3339 TCTAGA T^CTAGA XmnI 2453 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 1580 GACGTC G[GA]^CG[CT]C 2076 GACGCC AseI 1620 ATTAAT AT^TAAT 1755 ATTAAT AsnI 1620 ATTAAT AT^TAAT 1755 ATTAAT AvaI 140 CCCGAG C^[CT]CG[GA]G 3080 CCCGAG BamHI 1176 GGATCC G^GATCC 2429 GGATCC BanII 9 GGGCTC G[GA]GC[CT]^C 2364 GAGCTC BclI 1019 TGATCA T^GATCA 2149 TGATCA BcnI 556 CCCGG CC^[GC]GG 2158 CCCGG BsaHI 1580 GACGTC G[GA]^CG[CT]C 2076 GACGCC BsgI 2839 CTGCAC GTGCAG(16/14) 1449 GTGCAG BsoBI 140 CCCGAG C^[CT]CG[GA]G 3080 CCCGAG BspMI 184 GCAGGT ACCTGC(4/8) 1451 GCAGGT BsrBI 1369 CCGCTC CCGCTC(-3/-3) 213 GAGCGG DraII 1130 AGGACCT [GA]G^G[ACGT]CC[CT] 3151 AGGACCT EaeI 179 TGGCCG [CT]^GGCC[GA] 238 CGGCCA EcoO109I 1130 AGGACCT [GA]G^G[ACGT]CC[CT] 3151 AGGACCT FbaI 1019 TGATCA T^GATCA 2149 TGATCA Hin1I 1580 GACGTC G[GA]^CG[CT]C 2076 GACGCC MaeII 856 ACGT A^CGT 1581 ACGT MspA1I 256 CCGCTG C[AC]G^C[GT]G 2029 CAGCTG NciI 556 CCCGG CC^[GC]GG 2158 CCCGG NspI 200 GCATGC [GA]CATG^[CT] 282 ACATGC PpuMI 1130 AGGACCT [GA]G^G[AT]CC[CT] 3151 AGGACCT PshBI 1620 ATTAAT AT^TAAT 1755 ATTAAT SfcI 884 CTATAG C^T[GA][CT]AG 2032 CTGCAG StuI 1260 AGGCCT AGG^CCT 3084 AGGCCT XcmI 437 CCATTGGTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2998 CCACTTTCCTATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraIII EarI EcoRI EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII