##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvrII BalI BbeI BbvCI BciVI
BfrI BlnI BseAI BsiEI BsiWI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CpoI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT14I EcoT22I FauI FseI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I StyI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 1580 GACGTC GACGT^C
AccI 2522 GTCTAC GT^[AC][GT]AC
AocI 2127 CCTCAGG CC^T[ACGT]AGG
Asp700I 2453 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 193 TGCGCA TGC^GCA
BanI 1595 GGCACC G^G[CT][GA]CC
BbrPI 855 CACGTG CAC^GTG
BcgI 3082 CGAGGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 67 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1322 AGATCT A^GATCT
BlpI 155 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 155 GCTGAGC GC^T[ACGT]AGC
BsaAI 855 CACGTG [CT]AC^GT[GA]
BsaBI 1014 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2015 TCCGGT [AT]^CCGG[AT]
BsmBI 167 GAGACG CGTCTC(1/5)
Bsp1407I 3187 TGTACA T^GTACA
BspDI 1153 ATCGAT AT^CGAT
BsrFI 67 GCCGGC [GA]^CCGG[CT]
BsrGI 3187 TGTACA T^GTACA
Bsu36I 2127 CCTCAGG CC^T[ACGT]AGG
CelII 155 GCTGAGC GC^T[ACGT]AGC
Cfr10I 67 GCCGGC [GA]^CCGG[CT]
ClaI 1153 ATCGAT AT^CGAT
DraI 3329 TTTAAA TTT^AAA
DrdI 1752 GACATTAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2127 CCTCAGG CC^T[ACGT]AGG
EcoNI 3257 CCTGTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 193 TGCGCA TGC^GCA
HaeII 1443 GGCGCT [GA]GCGC^[CT]
HindIII 1695 AAGCTT A^AGCTT
HpaI 992 GTTAAC GTT^AAC
MamI 1014 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 67 GCCGGC GCC^GGC
NgoMIV 67 GCCGGC G^CCGGC
PflMI 437 CCATTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 855 CACGTG CAC^GTG
PmlI 855 CACGTG CAC^GTG
PstI 2032 CTGCAG CTGCA^G
PvuII 2029 CAGCTG CAG^CTG
SacI 2364 GAGCTC GAGCT^C
SapI 3092 GCTCTTC GCTCTTC(1/4)
ScaI 1965 AGTACT AGT^ACT
SexAI 1632 ACCAGGT A^CC[AT]GGT
SmlI 1879 CTCAAG C^T[CT][GA]AG
SpeI 2611 ACTAGT A^CTAGT
SphI 200 GCATGC GCATG^C
SspBI 3187 TGTACA T^GTACA
SspI 1866 AATATT AAT^ATT
Van91I 437 CCATTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3339 TCTAGA T^CTAGA
XmnI 2453 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1580 GACGTC G[GA]^CG[CT]C
2076 GACGCC
AseI 1620 ATTAAT AT^TAAT
1755 ATTAAT
AsnI 1620 ATTAAT AT^TAAT
1755 ATTAAT
AvaI 140 CCCGAG C^[CT]CG[GA]G
3080 CCCGAG
BamHI 1176 GGATCC G^GATCC
2429 GGATCC
BanII 9 GGGCTC G[GA]GC[CT]^C
2364 GAGCTC
BclI 1019 TGATCA T^GATCA
2149 TGATCA
BcnI 556 CCCGG CC^[GC]GG
2158 CCCGG
BsaHI 1580 GACGTC G[GA]^CG[CT]C
2076 GACGCC
BsgI 2839 CTGCAC GTGCAG(16/14)
1449 GTGCAG
BsoBI 140 CCCGAG C^[CT]CG[GA]G
3080 CCCGAG
BspMI 184 GCAGGT ACCTGC(4/8)
1451 GCAGGT
BsrBI 1369 CCGCTC CCGCTC(-3/-3)
213 GAGCGG
DraII 1130 AGGACCT [GA]G^G[ACGT]CC[CT]
3151 AGGACCT
EaeI 179 TGGCCG [CT]^GGCC[GA]
238 CGGCCA
EcoO109I 1130 AGGACCT [GA]G^G[ACGT]CC[CT]
3151 AGGACCT
FbaI 1019 TGATCA T^GATCA
2149 TGATCA
Hin1I 1580 GACGTC G[GA]^CG[CT]C
2076 GACGCC
MaeII 856 ACGT A^CGT
1581 ACGT
MspA1I 256 CCGCTG C[AC]G^C[GT]G
2029 CAGCTG
NciI 556 CCCGG CC^[GC]GG
2158 CCCGG
NspI 200 GCATGC [GA]CATG^[CT]
282 ACATGC
PpuMI 1130 AGGACCT [GA]G^G[AT]CC[CT]
3151 AGGACCT
PshBI 1620 ATTAAT AT^TAAT
1755 ATTAAT
SfcI 884 CTATAG C^T[GA][CT]AG
2032 CTGCAG
StuI 1260 AGGCCT AGG^CCT
3084 AGGCCT
XcmI 437 CCATTGGTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2998 CCACTTTCCTATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraIII
EarI EcoRI EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII