##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AcyI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BspMI BsrBI BssHII BstBI
BstEII BstPI CelII ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FauI
FseI FspI HaeII Hin1I KasI
KpnI KspI MluI MroI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshBI
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 4113 CGCG CG^CG
AclI 3311 AACGTT AA^CGTT
AflII 3602 CTTAAG C^TTAAG
AflIII 3318 ACATGT A^C[GA][CT]GT
AgeI 3864 ACCGGT A^CCGGT
AhdI 3045 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1597 CCTTAGG CC^T[ACGT]AGG
ApaI 812 GGGCCC GGGCC^C
AspEI 3045 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 3989 GGATAC GTATCC(6/5)
BclI 826 TGATCA T^GATCA
BfrI 3602 CTTAAG C^TTAAG
BglII 2770 AGATCT A^GATCT
BsaWI 3864 ACCGGT [AT]^CCGG[AT]
BseRI 2458 GAGGAG GAGGAG(10/8)
BsmBI 3155 CGTCTC CGTCTC(1/5)
BsmI 191 GAATGC GAATGC(1/-1)
Bsp1407I 2618 TGTACA T^GTACA
BspLU11I 3318 ACATGT A^CATGT
BsrDI 1344 GCAATG GCAATG(2/0)
BsrFI 3864 ACCGGT [GA]^CCGG[CT]
BsrGI 2618 TGTACA T^GTACA
BssSI 480 CACGAG CACGAG(-5/-1)
Bst1107I 122 GTATAC GTA^TAC
BstUI 4113 CGCG CG^CG
BstXI 3423 CCACATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 122 GTATAC GTA^TAC
Bsu36I 1597 CCTTAGG CC^T[ACGT]AGG
Cfr10I 3864 ACCGGT [GA]^CCGG[CT]
DraIII 1464 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3045 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1597 CCTTAGG CC^T[ACGT]AGG
EcoT22I 1630 ATGCAT ATGCA^T
FbaI 826 TGATCA T^GATCA
HpaI 3314 GTTAAC GTT^AAC
MvnI 4113 CGCG CG^CG
NsiI 1630 ATGCAT ATGCA^T
PinAI 3864 ACCGGT A^CCGGT
Psp1406I 3311 AACGTT AA^CGTT
SexAI 4053 ACCAGGT A^CC[AT]GGT
SspBI 2618 TGTACA T^GTACA
StuI 754 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Asp700I 3027 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3613 GAACACCTTC
AvaII 3867 GGTCC G^G[AT]CC
4178 GGTCC
BalI 1166 TGGCCA TGG^CCA
1471 TGGCCA
BanI 797 GGCACC G^G[CT][GA]CC
2042 GGTGCC
BbsI 1667 GAAGAC GAAGAC(2/6)
3053 GTCTTC
BbvCI 4182 CCTCAGC CCTCAGC(-5/-2)
382 GCTGAGG
BcnI 743 CCCGG CC^[GC]GG
2453 CCGGG
BpuAI 1667 GAAGAC GAAGAC(2/6)
3053 GTCTTC
CfoI 1866 GCGC GCG^C
4112 GCGC
EaeI 1166 TGGCCA [CT]^GGCC[GA]
1471 TGGCCA
HgaI 2414 GACGC GACGC(5/10)
3128 GCGTC
HhaI 1866 GCGC GCG^C
4112 GCGC
HinP1I 1866 GCGC G^CGC
4112 GCGC
HincII 3314 GTTAAC GT[CT]^[GA]AC
4205 GTTGAC
HindII 3314 GTTAAC GT[CT]^[GA]AC
4205 GTTGAC
HindIII 3879 AAGCTT A^AGCTT
4371 AAGCTT
MaeII 3312 ACGT A^CGT
4044 ACGT
MfeI 1608 CAATTG C^AATTG
4038 CAATTG
MluNI 1166 TGGCCA TGG^CCA
1471 TGGCCA
MscI 1166 TGGCCA TGG^CCA
1471 TGGCCA
MunI 1608 CAATTG C^AATTG
4038 CAATTG
NciI 743 CCCGG CC^[GC]GG
2453 CCGGG
NheI 1989 GCTAGC G^CTAGC
2164 GCTAGC
PstI 2234 CTGCAG CTGCA^G
3981 CTGCAG
SapI 2517 GAAGAGC GCTCTTC(1/4)
3775 GAAGAGC
SphI 2250 GCATGC GCATG^C
2573 GCATGC
TaqI 149 TCGA T^CGA
1812 TCGA
TthHB8I 149 TCGA T^CGA
1812 TCGA
XcmI 70 CCAGAACAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
590 CCAGAAACAAGCTGG
XmnI 3027 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3613 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI BanII
BbvI BfaI BmyI BpmI BsaBI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PshAI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII