################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccIII AcyI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BsiEI BsiWI BsoBI BspDI BspEI BspMI BsrBI BssHII BstBI BstEII BstPI CelII ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FauI FseI FspI HaeII Hin1I KasI KpnI KspI MluI MroI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshBI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 4113 CGCG CG^CG AclI 3311 AACGTT AA^CGTT AflII 3602 CTTAAG C^TTAAG AflIII 3318 ACATGT A^C[GA][CT]GT AgeI 3864 ACCGGT A^CCGGT AhdI 3045 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1597 CCTTAGG CC^T[ACGT]AGG ApaI 812 GGGCCC GGGCC^C AspEI 3045 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BciVI 3989 GGATAC GTATCC(6/5) BclI 826 TGATCA T^GATCA BfrI 3602 CTTAAG C^TTAAG BglII 2770 AGATCT A^GATCT BsaWI 3864 ACCGGT [AT]^CCGG[AT] BseRI 2458 GAGGAG GAGGAG(10/8) BsmBI 3155 CGTCTC CGTCTC(1/5) BsmI 191 GAATGC GAATGC(1/-1) Bsp1407I 2618 TGTACA T^GTACA BspLU11I 3318 ACATGT A^CATGT BsrDI 1344 GCAATG GCAATG(2/0) BsrFI 3864 ACCGGT [GA]^CCGG[CT] BsrGI 2618 TGTACA T^GTACA BssSI 480 CACGAG CACGAG(-5/-1) Bst1107I 122 GTATAC GTA^TAC BstUI 4113 CGCG CG^CG BstXI 3423 CCACATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 122 GTATAC GTA^TAC Bsu36I 1597 CCTTAGG CC^T[ACGT]AGG Cfr10I 3864 ACCGGT [GA]^CCGG[CT] DraIII 1464 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 3045 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1597 CCTTAGG CC^T[ACGT]AGG EcoT22I 1630 ATGCAT ATGCA^T FbaI 826 TGATCA T^GATCA HpaI 3314 GTTAAC GTT^AAC MvnI 4113 CGCG CG^CG NsiI 1630 ATGCAT ATGCA^T PinAI 3864 ACCGGT A^CCGGT Psp1406I 3311 AACGTT AA^CGTT SexAI 4053 ACCAGGT A^CC[AT]GGT SspBI 2618 TGTACA T^GTACA StuI 754 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Asp700I 3027 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3613 GAACACCTTC AvaII 3867 GGTCC G^G[AT]CC 4178 GGTCC BalI 1166 TGGCCA TGG^CCA 1471 TGGCCA BanI 797 GGCACC G^G[CT][GA]CC 2042 GGTGCC BbsI 1667 GAAGAC GAAGAC(2/6) 3053 GTCTTC BbvCI 4182 CCTCAGC CCTCAGC(-5/-2) 382 GCTGAGG BcnI 743 CCCGG CC^[GC]GG 2453 CCGGG BpuAI 1667 GAAGAC GAAGAC(2/6) 3053 GTCTTC CfoI 1866 GCGC GCG^C 4112 GCGC EaeI 1166 TGGCCA [CT]^GGCC[GA] 1471 TGGCCA HgaI 2414 GACGC GACGC(5/10) 3128 GCGTC HhaI 1866 GCGC GCG^C 4112 GCGC HinP1I 1866 GCGC G^CGC 4112 GCGC HincII 3314 GTTAAC GT[CT]^[GA]AC 4205 GTTGAC HindII 3314 GTTAAC GT[CT]^[GA]AC 4205 GTTGAC HindIII 3879 AAGCTT A^AGCTT 4371 AAGCTT MaeII 3312 ACGT A^CGT 4044 ACGT MfeI 1608 CAATTG C^AATTG 4038 CAATTG MluNI 1166 TGGCCA TGG^CCA 1471 TGGCCA MscI 1166 TGGCCA TGG^CCA 1471 TGGCCA MunI 1608 CAATTG C^AATTG 4038 CAATTG NciI 743 CCCGG CC^[GC]GG 2453 CCGGG NheI 1989 GCTAGC G^CTAGC 2164 GCTAGC PstI 2234 CTGCAG CTGCA^G 3981 CTGCAG SapI 2517 GAAGAGC GCTCTTC(1/4) 3775 GAAGAGC SphI 2250 GCATGC GCATG^C 2573 GCATGC TaqI 149 TCGA T^CGA 1812 TCGA TthHB8I 149 TCGA T^CGA 1812 TCGA XcmI 70 CCAGAACAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 590 CCAGAAACAAGCTGG XmnI 3027 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3613 GAACACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI BanII BbvI BfaI BmyI BpmI BsaBI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PshAI PvuII RcaI RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII