##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BalI BbeI BbrPI BcgI
BciVI BglI BlnI BsaAI BsaWI
BseAI BsiEI BsiWI BsmFI BspDI
BspEI BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I Cfr10I
ClaI CpoI DraII DraIII DsaI
EaeI EagI Eam1105I EclXI Eco52I
EcoO109I EcoRI EcoRV FauI FseI
FspI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NgoMIV NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 4590 CGCG CG^CG
AcyI 1 GGCGTC G[GA]^CG[CT]C
AocI 463 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1217 AGCGCT AGC^GCT
Asp700I 4058 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1058 CTCGAG C^[CT]CG[GA]G
BamHI 858 GGATCC G^GATCC
BanI 3060 GGTGCC G^G[CT][GA]CC
BbvCI 3718 CCTCAGC CCTCAGC(-5/-2)
BclI 843 TGATCA T^GATCA
BcnI 1681 CCGGG CC^[GC]GG
BglII 3250 AGATCT A^GATCT
BsaBI 1132 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1 GGCGTC G[GA]^CG[CT]C
BsmBI 3 CGTCTC CGTCTC(1/5)
BsoBI 1058 CTCGAG C^[CT]CG[GA]G
BsrDI 4165 CATTGC GCAATG(2/0)
BssSI 418 CACGAG CACGAG(-5/-1)
BstUI 4590 CGCG CG^CG
Bsu36I 463 CCTGAGG CC^T[ACGT]AGG
DrdI 2383 GACTGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1217 AGCGCT AGC^GCT
Eco81I 463 CCTGAGG CC^T[ACGT]AGG
EcoT14I 2867 CCAAGG C^C[AT][AT]GG
FbaI 843 TGATCA T^GATCA
HapII 1681 CCGG C^CGG
Hin1I 1 GGCGTC G[GA]^CG[CT]C
HpaII 1681 CCGG C^CGG
MamI 1132 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1681 CCGG C^CGG
MvnI 4590 CGCG CG^CG
NciI 1681 CCGGG CC^[GC]GG
NheI 2208 GCTAGC G^CTAGC
PaeR7I 1058 CTCGAG C^TCGAG
PstI 4143 CTGCAG CTGCA^G
SexAI 2839 ACCAGGT A^CC[AT]GGT
StyI 2867 CCAAGG C^C[AT][AT]GG
XbaI 910 TCTAGA T^CTAGA
XhoI 1058 CTCGAG C^TCGAG
XmnI 4058 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 1199 ACATGT A^C[GA][CT]GT
1488 ACATGT
AseI 2701 ATTAAT AT^TAAT
3521 ATTAAT
AsnI 2701 ATTAAT AT^TAAT
3521 ATTAAT
BanII 768 GGGCTC G[GA]GC[CT]^C
1683 GGGCTC
BpmI 6 CTCCAG CTGGAG(16/14)
3132 CTCCAG
BsaI 1635 GAGACC GGTCTC(1/5)
3834 GGTCTC
BsgI 2005 CTGCAC GTGCAG(16/14)
3038 CTGCAC
Bsp1407I 2433 TGTACA T^GTACA
4114 TGTACA
BspLU11I 1199 ACATGT A^CATGT
1488 ACATGT
BsrGI 2433 TGTACA T^GTACA
4114 TGTACA
BstEII 458 GGTTACC G^GT[ACGT]ACC
1821 GGTGACC
BstPI 458 GGTTACC G^GT[ACGT]ACC
1821 GGTGACC
CfoI 1218 GCGC GCG^C
1400 GCGC
EcoO65I 458 GGTTACC G^GT[ACGT]ACC
1821 GGTGACC
EcoT22I 3417 ATGCAT ATGCA^T
4049 ATGCAT
HaeII 1217 AGCGCT [GA]GCGC^[CT]
1399 GGCGCT
HgaI 2 GCGTC GACGC(5/10)
907 GCGTC
HhaI 1218 GCGC GCG^C
1400 GCGC
HinP1I 1218 GCGC G^CGC
1400 GCGC
HindIII 2410 AAGCTT A^AGCTT
2670 AAGCTT
HpaI 1858 GTTAAC GTT^AAC
2537 GTTAAC
NsiI 3417 ATGCAT ATGCA^T
4049 ATGCAT
PflMI 76 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1501 CCAACAGGTGG
PshBI 2701 ATTAAT AT^TAAT
3521 ATTAAT
PvuII 293 CAGCTG CAG^CTG
3112 CAGCTG
SfcI 540 CTGTAG C^T[GA][CT]AG
4143 CTGCAG
SspBI 2433 TGTACA T^GTACA
4114 TGTACA
Tsp45I 1822 GTGAC ^GT[GC]AC
3825 GTCAC
Van91I 76 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1501 CCAACAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AspHI AvaII
BbsI BbvI BfaI BfrI BlpI
BmyI Bpu1102I BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
Cfr13I DdeI DpnII DraI EarI
EcoNI EcoRII Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI SspI StuI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII