################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII Acc65I AccII AcyI AgeI AhdI Aor51HI AscI Asp718I AspEI AviII BamHI BbeI BbrPI BcgI BglI BsaAI BsaBI BsaHI BsiEI BsiWI Bsp1407I BspDI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 1391 TCCGGA T^CCGGA Alw44I 3434 GTGCAC G^TGCAC AocI 874 CCTGAGG CC^T[ACGT]AGG ApaLI 3434 GTGCAC G^TGCAC Asp700I 2497 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 440 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1159 CCTAGG C^CTAGG BalI 1835 TGGCCA TGG^CCA BanI 1823 GGTGCC G^G[CT][GA]CC BciVI 2207 GTATCC GTATCC(6/5) BcnI 24 CCCGG CC^[GC]GG BlnI 1159 CCTAGG C^CTAGG BlpI 4058 GCTTAGC GC^T[ACGT]AGC Bpu1102I 4058 GCTTAGC GC^T[ACGT]AGC BseAI 1391 TCCGGA T^CCGGA BsmI 4020 GAATGC GAATGC(1/-1) BspEI 1391 TCCGGA T^CCGGA BspMI 2710 ACCTGC ACCTGC(4/8) BsrBI 3380 CCGCTC CCGCTC(-3/-3) BssSI 4311 CTCGTG CACGAG(-5/-1) BstEII 2801 GGTTACC G^GT[ACGT]ACC BstPI 2801 GGTTACC G^GT[ACGT]ACC Bsu36I 874 CCTGAGG CC^T[ACGT]AGG CelII 4058 GCTTAGC GC^T[ACGT]AGC CfoI 3865 GCGC GCG^C DraIII 4220 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1835 TGGCCA [CT]^GGCC[GA] Eco81I 874 CCTGAGG CC^T[ACGT]AGG EcoO65I 2801 GGTTACC G^GT[ACGT]ACC EcoRV 5048 GATATC GAT^ATC EcoT22I 33 ATGCAT ATGCA^T HhaI 3865 GCGC GCG^C HinP1I 3865 GCGC G^CGC HincII 1579 GTCAAC GT[CT]^[GA]AC HindII 1579 GTCAAC GT[CT]^[GA]AC MluNI 1835 TGGCCA TGG^CCA MroI 1391 TCCGGA T^CCGGA MscI 1835 TGGCCA TGG^CCA NciI 24 CCCGG CC^[GC]GG NdeI 3536 CATATG CA^TATG NsiI 33 ATGCAT ATGCA^T PaeR7I 2450 CTCGAG C^TCGAG PflFI 440 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 4398 GAGCTC GAGCT^C SapI 1712 GAAGAGC GCTCTTC(1/4) ScaI 802 AGTACT AGT^ACT Tth111I 440 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 4089 CCACAGACTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2450 CTCGAG C^TCGAG XmnI 2497 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AclI 3096 AACGTT AA^CGTT 4914 AACGTT AflII 2230 CTTAAG C^TTAAG 2933 CTTAAG AflIII 4447 ACATGT A^C[GA][CT]GT 5005 ACATGT ApaI 1155 GGGCCC GGGCC^C 1765 GGGCCC AseI 1122 ATTAAT AT^TAAT 3050 ATTAAT AsnI 1122 ATTAAT AT^TAAT 3050 ATTAAT BfrI 2230 CTTAAG C^TTAAG 2933 CTTAAG BglII 3066 AGATCT A^GATCT 3112 AGATCT BsaI 975 GAGACC GGTCTC(1/5) 3241 GGTCTC BseRI 4406 CTCCTC GAGGAG(10/8) 1 GAGGAG BsgI 3821 GTGCAG GTGCAG(16/14) 4314 GTGCAG BsmBI 715 GAGACG CGTCTC(1/5) 4350 GAGACG BspLU11I 4447 ACATGT A^CATGT 5005 ACATGT BsrDI 1438 GCAATG GCAATG(2/0) 1559 GCAATG BstXI 1195 CCACCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1651 CCAGAAGGCTGG DraI 3699 TTTAAA TTT^AAA 4834 TTTAAA DrdI 588 GACCCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3826 GACTCTGCAGTC DsaI 356 CCATGG C^C[GA][CT]GG 2147 CCATGG EcoNI 3886 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4118 CCTCAGCCAGG HgaI 886 GCGTC GACGC(5/10) 3844 GCGTC NcoI 356 CCATGG C^CATGG 2147 CCATGG PshBI 1122 ATTAAT AT^TAAT 3050 ATTAAT Psp1406I 3096 AACGTT AA^CGTT 4914 AACGTT StuI 1162 AGGCCT AGG^CCT 4584 AGGCCT XbaI 955 TCTAGA T^CTAGA 4457 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbsI BbvCI BbvI BclI BfaI BmyI BpmI BpuAI BsaJI BsaWI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII