##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AccII       AcyI        AgeI        
AhdI        Aor51HI     AscI        Asp718I     AspEI       
AviII       BamHI       BbeI        BbrPI       BcgI        
BglI        BsaAI       BsaBI       BsaHI       BsiEI       
BsiWI       Bsp1407I    BspDI       BsrFI       BsrGI       
BssHII      Bst1107I    BstBI       BstUI       BstZ17I     
Cfr10I      ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      FseI        FspI        
HaeII       Hin1I       HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII       1391  TCCGGA               T^CCGGA
Alw44I       3434  GTGCAC               G^TGCAC
AocI          874  CCTGAGG              CC^T[ACGT]AGG
ApaLI        3434  GTGCAC               G^TGCAC
Asp700I      2497  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          440  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1159  CCTAGG               C^CTAGG
BalI         1835  TGGCCA               TGG^CCA
BanI         1823  GGTGCC               G^G[CT][GA]CC
BciVI        2207  GTATCC               GTATCC(6/5)
BcnI           24  CCCGG                CC^[GC]GG
BlnI         1159  CCTAGG               C^CTAGG
BlpI         4058  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     4058  GCTTAGC              GC^T[ACGT]AGC
BseAI        1391  TCCGGA               T^CCGGA
BsmI         4020  GAATGC               GAATGC(1/-1)
BspEI        1391  TCCGGA               T^CCGGA
BspMI        2710  ACCTGC               ACCTGC(4/8)
BsrBI        3380  CCGCTC               CCGCTC(-3/-3)
BssSI        4311  CTCGTG               CACGAG(-5/-1)
BstEII       2801  GGTTACC              G^GT[ACGT]ACC
BstPI        2801  GGTTACC              G^GT[ACGT]ACC
Bsu36I        874  CCTGAGG              CC^T[ACGT]AGG
CelII        4058  GCTTAGC              GC^T[ACGT]AGC
CfoI         3865  GCGC                 GCG^C
DraIII       4220  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1835  TGGCCA               [CT]^GGCC[GA]
Eco81I        874  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      2801  GGTTACC              G^GT[ACGT]ACC
EcoRV        5048  GATATC               GAT^ATC
EcoT22I        33  ATGCAT               ATGCA^T
HhaI         3865  GCGC                 GCG^C
HinP1I       3865  GCGC                 G^CGC
HincII       1579  GTCAAC               GT[CT]^[GA]AC
HindII       1579  GTCAAC               GT[CT]^[GA]AC
MluNI        1835  TGGCCA               TGG^CCA
MroI         1391  TCCGGA               T^CCGGA
MscI         1835  TGGCCA               TGG^CCA
NciI           24  CCCGG                CC^[GC]GG
NdeI         3536  CATATG               CA^TATG
NsiI           33  ATGCAT               ATGCA^T
PaeR7I       2450  CTCGAG               C^TCGAG
PflFI         440  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacI         4398  GAGCTC               GAGCT^C
SapI         1712  GAAGAGC              GCTCTTC(1/4)
ScaI          802  AGTACT               AGT^ACT
Tth111I       440  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         4089  CCACAGACTTTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2450  CTCGAG               C^TCGAG
XmnI         2497  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AclI         3096  AACGTT               AA^CGTT
             4914  AACGTT              
AflII        2230  CTTAAG               C^TTAAG
             2933  CTTAAG              
AflIII       4447  ACATGT               A^C[GA][CT]GT
             5005  ACATGT              
ApaI         1155  GGGCCC               GGGCC^C
             1765  GGGCCC              
AseI         1122  ATTAAT               AT^TAAT
             3050  ATTAAT              
AsnI         1122  ATTAAT               AT^TAAT
             3050  ATTAAT              
BfrI         2230  CTTAAG               C^TTAAG
             2933  CTTAAG              
BglII        3066  AGATCT               A^GATCT
             3112  AGATCT              
BsaI          975  GAGACC               GGTCTC(1/5)
             3241  GGTCTC              
BseRI        4406  CTCCTC               GAGGAG(10/8)
                1  GAGGAG              
BsgI         3821  GTGCAG               GTGCAG(16/14)
             4314  GTGCAG              
BsmBI         715  GAGACG               CGTCTC(1/5)
             4350  GAGACG              
BspLU11I     4447  ACATGT               A^CATGT
             5005  ACATGT              
BsrDI        1438  GCAATG               GCAATG(2/0)
             1559  GCAATG              
BstXI        1195  CCACCTGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1651  CCAGAAGGCTGG        
DraI         3699  TTTAAA               TTT^AAA
             4834  TTTAAA              
DrdI          588  GACCCTGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3826  GACTCTGCAGTC        
DsaI          356  CCATGG               C^C[GA][CT]GG
             2147  CCATGG              
EcoNI        3886  CCTGACACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4118  CCTCAGCCAGG         
HgaI          886  GCGTC                GACGC(5/10)
             3844  GCGTC               
NcoI          356  CCATGG               C^CATGG
             2147  CCATGG              
PshBI        1122  ATTAAT               AT^TAAT
             3050  ATTAAT              
Psp1406I     3096  AACGTT               AA^CGTT
             4914  AACGTT              
StuI         1162  AGGCCT               AGG^CCT
             4584  AGGCCT              
XbaI          955  TCTAGA               T^CTAGA
             4457  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BanII       BbsI        BbvCI       BbvI        
BclI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BsaWI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspHI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeIII      HapII       HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII