##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AcyI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII BamHI BbeI BbrPI BcgI
BglI BsaAI BsaBI BsaHI BsiEI
BsiWI Bsp1407I BspDI BsrFI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I FseI FspI
HaeII Hin1I HpaI KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 1391 TCCGGA T^CCGGA
Alw44I 3434 GTGCAC G^TGCAC
AocI 874 CCTGAGG CC^T[ACGT]AGG
ApaLI 3434 GTGCAC G^TGCAC
Asp700I 2497 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 440 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1159 CCTAGG C^CTAGG
BalI 1835 TGGCCA TGG^CCA
BanI 1823 GGTGCC G^G[CT][GA]CC
BciVI 2207 GTATCC GTATCC(6/5)
BcnI 24 CCCGG CC^[GC]GG
BlnI 1159 CCTAGG C^CTAGG
BlpI 4058 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4058 GCTTAGC GC^T[ACGT]AGC
BseAI 1391 TCCGGA T^CCGGA
BsmI 4020 GAATGC GAATGC(1/-1)
BspEI 1391 TCCGGA T^CCGGA
BspMI 2710 ACCTGC ACCTGC(4/8)
BsrBI 3380 CCGCTC CCGCTC(-3/-3)
BssSI 4311 CTCGTG CACGAG(-5/-1)
BstEII 2801 GGTTACC G^GT[ACGT]ACC
BstPI 2801 GGTTACC G^GT[ACGT]ACC
Bsu36I 874 CCTGAGG CC^T[ACGT]AGG
CelII 4058 GCTTAGC GC^T[ACGT]AGC
CfoI 3865 GCGC GCG^C
DraIII 4220 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1835 TGGCCA [CT]^GGCC[GA]
Eco81I 874 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2801 GGTTACC G^GT[ACGT]ACC
EcoRV 5048 GATATC GAT^ATC
EcoT22I 33 ATGCAT ATGCA^T
HhaI 3865 GCGC GCG^C
HinP1I 3865 GCGC G^CGC
HincII 1579 GTCAAC GT[CT]^[GA]AC
HindII 1579 GTCAAC GT[CT]^[GA]AC
MluNI 1835 TGGCCA TGG^CCA
MroI 1391 TCCGGA T^CCGGA
MscI 1835 TGGCCA TGG^CCA
NciI 24 CCCGG CC^[GC]GG
NdeI 3536 CATATG CA^TATG
NsiI 33 ATGCAT ATGCA^T
PaeR7I 2450 CTCGAG C^TCGAG
PflFI 440 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 4398 GAGCTC GAGCT^C
SapI 1712 GAAGAGC GCTCTTC(1/4)
ScaI 802 AGTACT AGT^ACT
Tth111I 440 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4089 CCACAGACTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2450 CTCGAG C^TCGAG
XmnI 2497 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AclI 3096 AACGTT AA^CGTT
4914 AACGTT
AflII 2230 CTTAAG C^TTAAG
2933 CTTAAG
AflIII 4447 ACATGT A^C[GA][CT]GT
5005 ACATGT
ApaI 1155 GGGCCC GGGCC^C
1765 GGGCCC
AseI 1122 ATTAAT AT^TAAT
3050 ATTAAT
AsnI 1122 ATTAAT AT^TAAT
3050 ATTAAT
BfrI 2230 CTTAAG C^TTAAG
2933 CTTAAG
BglII 3066 AGATCT A^GATCT
3112 AGATCT
BsaI 975 GAGACC GGTCTC(1/5)
3241 GGTCTC
BseRI 4406 CTCCTC GAGGAG(10/8)
1 GAGGAG
BsgI 3821 GTGCAG GTGCAG(16/14)
4314 GTGCAG
BsmBI 715 GAGACG CGTCTC(1/5)
4350 GAGACG
BspLU11I 4447 ACATGT A^CATGT
5005 ACATGT
BsrDI 1438 GCAATG GCAATG(2/0)
1559 GCAATG
BstXI 1195 CCACCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1651 CCAGAAGGCTGG
DraI 3699 TTTAAA TTT^AAA
4834 TTTAAA
DrdI 588 GACCCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3826 GACTCTGCAGTC
DsaI 356 CCATGG C^C[GA][CT]GG
2147 CCATGG
EcoNI 3886 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4118 CCTCAGCCAGG
HgaI 886 GCGTC GACGC(5/10)
3844 GCGTC
NcoI 356 CCATGG C^CATGG
2147 CCATGG
PshBI 1122 ATTAAT AT^TAAT
3050 ATTAAT
Psp1406I 3096 AACGTT AA^CGTT
4914 AACGTT
StuI 1162 AGGCCT AGG^CCT
4584 AGGCCT
XbaI 955 TCTAGA T^CTAGA
4457 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbsI BbvCI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII