##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AccIII AclI AflII Aor51HI ApaI
AscI AviII BamHI BbeI BbvCI
BfrI BlpI Bpu1102I BseAI BspEI
BsrDI BssSI BstBI CelII CpoI
Eco47III EcoRV FseI FspI HaeII
HpaI KasI MluI MroI NarI
NdeI NheI NruI NspV PacI
PmeI Psp1406I RsrII SacI SalI
SapI SbfI SfiI SfoI SfuI
SmaI SpeI SphI Sse8387I SspI
StuI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 3632 GACGTC GACGT^C
Acc65I 3178 GGTACC G^GTACC
AgeI 3175 ACCGGT A^CCGGT
Alw44I 4615 GTGCAC G^TGCAC
ApaLI 4615 GTGCAC G^TGCAC
AseI 4913 ATTAAT AT^TAAT
AsnI 4913 ATTAAT AT^TAAT
Asp718I 3178 GGTACC G^GTACC
AspI 2294 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4372 TGGCCA TGG^CCA
BanI 3178 GGTACC G^G[CT][GA]CC
BanII 1979 GAGCCC G[GA]GC[CT]^C
BbrPI 4717 CACGTG CAC^GTG
BcgI 3892 CGATGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1821 GTATCC GTATCC(6/5)
BclI 191 TGATCA T^GATCA
BglI 105 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiWI 4390 CGTACG C^GTACG
BsmBI 1079 GAGACG CGTCTC(1/5)
BsmI 2834 GAATGC GAATGC(1/-1)
BspDI 393 ATCGAT AT^CGAT
BspLU11I 1767 ACATGT A^CATGT
Bst1107I 3949 GTATAC GTA^TAC
BstEII 5278 GGTGACC G^GT[ACGT]ACC
BstPI 5278 GGTGACC G^GT[ACGT]ACC
BstZ17I 3949 GTATAC GTA^TAC
ClaI 393 ATCGAT AT^CGAT
DraI 1553 TTTAAA TTT^AAA
DrdI 4789 GACGTGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5278 GGTGACC G^GT[ACGT]ACC
EcoRI 3398 GAATTC G^AATTC
EcoT22I 1009 ATGCAT ATGCA^T
FbaI 191 TGATCA T^GATCA
HincII 2346 GTTGAC GT[CT]^[GA]AC
HindII 2346 GTTGAC GT[CT]^[GA]AC
HindIII 2564 AAGCTT A^AGCTT
KpnI 3178 GGTACC GGTAC^C
MfeI 2469 CAATTG C^AATTG
MluNI 4372 TGGCCA TGG^CCA
MscI 4372 TGGCCA TGG^CCA
MunI 2469 CAATTG C^AATTG
NaeI 105 GCCGGC GCC^GGC
NcoI 57 CCATGG C^CATGG
NgoMIV 105 GCCGGC G^CCGGC
NotI 111 GCGGCCGC GC^GGCCGC
NsiI 1009 ATGCAT ATGCA^T
PaeR7I 1129 CTCGAG C^TCGAG
PflFI 2294 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3175 ACCGGT A^CCGGT
PmaCI 4717 CACGTG CAC^GTG
PmlI 4717 CACGTG CAC^GTG
PshAI 270 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4913 ATTAAT AT^TAAT
SexAI 5050 ACCAGGT A^CC[AT]GGT
SgrAI 104 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 4392 TACGTA TAC^GTA
Tth111I 2294 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1129 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 3632 GACGTC G[GA]^CG[CT]C
5508 GACGCC
AflIII 1767 ACATGT A^C[GA][CT]GT
4790 ACGTGT
AhdI 1139 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2505 GACAAGCTGTC
AspEI 1139 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2505 GACAAGCTGTC
AvaI 1129 CTCGAG C^[CT]CG[GA]G
2911 CCCGAG
AvrII 4456 CCTAGG C^CTAGG
5099 CCTAGG
BlnI 4456 CCTAGG C^CTAGG
5099 CCTAGG
BsaAI 4392 TACGTA [CT]AC^GT[GA]
4717 CACGTG
BsaBI 3049 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4548 GATGGAAATC
BsaHI 3632 GACGTC G[GA]^CG[CT]C
5508 GACGCC
BsaWI 3175 ACCGGT [AT]^CCGG[AT]
3181 ACCGGA
BsoBI 1129 CTCGAG C^[CT]CG[GA]G
2911 CCCGAG
BsrBI 3041 CCGCTC CCGCTC(-3/-3)
41 GAGCGG
BstAPI 1092 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5428 GCACTAACTGC
Eam1105I 1139 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2505 GACAAGCTGTC
HgaI 5508 GACGC GACGC(5/10)
5524 GCGTC
Hin1I 3632 GACGTC G[GA]^CG[CT]C
5508 GACGCC
KspI 20 CCGCGG CCGC^GG
115 CCGCGG
MamI 3049 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4548 GATGGAAATC
PvuI 395 CGATCG CGAT^CG
3048 CGATCG
SacII 20 CCGCGG CCGC^GG
115 CCGCGG
ScaI 1884 AGTACT AGT^ACT
1932 AGTACT
SmlI 1129 CTCGAG C^T[CT][GA]AG
2930 CTCAAG
XbaI 2604 TCTAGA T^CTAGA
2886 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspHI BspMI
BsrFI BsrGI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI