################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII Acc65I AccIII AclI AflII Aor51HI AscI AseI AsnI Asp700I Asp718I AviII AvrII BbeI BciVI BclI BfrI BlnI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoRV FbaI FseI FspI HpaI KasI KpnI KspI MfeI MluI MroI MunI NarI NheI NotI NruI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI SwaI TaqI TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 3108 GTAGAC GT^[AC][GT]AC AcyI 443 GACGCC G[GA]^CG[CT]C AhdI 1736 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 976 GTGCAC G^TGCAC ApaLI 976 GTGCAC G^TGCAC AspEI 1736 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 2600 GGATCC G^GATCC BbvCI 355 CCTCAGC CCTCAGC(-5/-2) BglII 147 AGATCT A^GATCT BlpI 1310 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1310 GCTCAGC GC^T[ACGT]AGC BsaHI 443 GACGCC G[GA]^CG[CT]C BsmI 925 GCATTC GAATGC(1/-1) Bsp1407I 1709 TGTACA T^GTACA BspHI 3906 TCATGA T^CATGA BsrGI 1709 TGTACA T^GTACA BssHII 2287 GCGCGC G^CGCGC BssSI 715 CTCGTG CACGAG(-5/-1) BstEII 1511 GGTTACC G^GT[ACGT]ACC BstPI 1511 GGTTACC G^GT[ACGT]ACC CelII 1310 GCTCAGC GC^T[ACGT]AGC Eam1105I 1736 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1511 GGTTACC G^GT[ACGT]ACC EcoRI 3679 GAATTC G^AATTC EcoT22I 3187 ATGCAT ATGCA^T Hin1I 443 GACGCC G[GA]^CG[CT]C NdeI 4185 CATATG CA^TATG NsiI 3187 ATGCAT ATGCA^T RcaI 3906 TCATGA T^CATGA SapI 3864 GAAGAGC GCTCTTC(1/4) SfiI 734 GGCCGCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 3449 CTCAAG C^T[CT][GA]AG SspBI 1709 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 1793 CGCG CG^CG 2288 CGCG AgeI 1809 ACCGGT A^CCGGT 4002 ACCGGT BcgI 499 GCAGTGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3569 GCAAGTCAGTCG BsaBI 1539 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4158 GATAGTGATC BsaWI 1809 ACCGGT [AT]^CCGG[AT] 4002 ACCGGT BsgI 423 GTGCAG GTGCAG(16/14) 544 GTGCAG BspLU11I 1082 ACATGT A^CATGT 2300 ACATGT BsrDI 305 CATTGC GCAATG(2/0) 3083 CATTGC BstAPI 666 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2764 GCAGGCTGTGC BstUI 1793 CGCG CG^CG 2288 CGCG FauI 1327 CCCGC CCCGC(4/6) 1716 GCGGG HincII 2103 GTCAAC GT[CT]^[GA]AC 3943 GTCAAC HindII 2103 GTCAAC GT[CT]^[GA]AC 3943 GTCAAC HindIII 616 AAGCTT A^AGCTT 2040 AAGCTT MamI 1539 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4158 GATAGTGATC MvnI 1793 CGCG CG^CG 2288 CGCG NaeI 1050 GCCGGC GCC^GGC 2063 GCCGGC NgoMIV 1050 GCCGGC G^CCGGC 2063 GCCGGC PinAI 1809 ACCGGT A^CCGGT 4002 ACCGGT PshAI 1133 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4084 GACCAAGGTC SmaI 1766 CCCGGG CCC^GGG 2668 CCCGGG SspI 3853 AATATT AAT^ATT 4327 AATATT XmaCI 1766 CCCGGG C^CCGGG 2668 CCCGGG XmaI 1766 CCCGGG C^CCGGG 2668 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI Sse8387I StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XcmI XhoII