##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
Aor51HI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BbeI BciVI
BclI BfrI BlnI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoRV FbaI FseI FspI
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NarI NheI
NotI NruI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RsrII
SacI SacII SalI ScaI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SphI SwaI TaqI TthHB8I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 3108 GTAGAC GT^[AC][GT]AC
AcyI 443 GACGCC G[GA]^CG[CT]C
AhdI 1736 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 976 GTGCAC G^TGCAC
ApaLI 976 GTGCAC G^TGCAC
AspEI 1736 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2600 GGATCC G^GATCC
BbvCI 355 CCTCAGC CCTCAGC(-5/-2)
BglII 147 AGATCT A^GATCT
BlpI 1310 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1310 GCTCAGC GC^T[ACGT]AGC
BsaHI 443 GACGCC G[GA]^CG[CT]C
BsmI 925 GCATTC GAATGC(1/-1)
Bsp1407I 1709 TGTACA T^GTACA
BspHI 3906 TCATGA T^CATGA
BsrGI 1709 TGTACA T^GTACA
BssHII 2287 GCGCGC G^CGCGC
BssSI 715 CTCGTG CACGAG(-5/-1)
BstEII 1511 GGTTACC G^GT[ACGT]ACC
BstPI 1511 GGTTACC G^GT[ACGT]ACC
CelII 1310 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1736 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1511 GGTTACC G^GT[ACGT]ACC
EcoRI 3679 GAATTC G^AATTC
EcoT22I 3187 ATGCAT ATGCA^T
Hin1I 443 GACGCC G[GA]^CG[CT]C
NdeI 4185 CATATG CA^TATG
NsiI 3187 ATGCAT ATGCA^T
RcaI 3906 TCATGA T^CATGA
SapI 3864 GAAGAGC GCTCTTC(1/4)
SfiI 734 GGCCGCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 3449 CTCAAG C^T[CT][GA]AG
SspBI 1709 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 1793 CGCG CG^CG
2288 CGCG
AgeI 1809 ACCGGT A^CCGGT
4002 ACCGGT
BcgI 499 GCAGTGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3569 GCAAGTCAGTCG
BsaBI 1539 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4158 GATAGTGATC
BsaWI 1809 ACCGGT [AT]^CCGG[AT]
4002 ACCGGT
BsgI 423 GTGCAG GTGCAG(16/14)
544 GTGCAG
BspLU11I 1082 ACATGT A^CATGT
2300 ACATGT
BsrDI 305 CATTGC GCAATG(2/0)
3083 CATTGC
BstAPI 666 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2764 GCAGGCTGTGC
BstUI 1793 CGCG CG^CG
2288 CGCG
FauI 1327 CCCGC CCCGC(4/6)
1716 GCGGG
HincII 2103 GTCAAC GT[CT]^[GA]AC
3943 GTCAAC
HindII 2103 GTCAAC GT[CT]^[GA]AC
3943 GTCAAC
HindIII 616 AAGCTT A^AGCTT
2040 AAGCTT
MamI 1539 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4158 GATAGTGATC
MvnI 1793 CGCG CG^CG
2288 CGCG
NaeI 1050 GCCGGC GCC^GGC
2063 GCCGGC
NgoMIV 1050 GCCGGC G^CCGGC
2063 GCCGGC
PinAI 1809 ACCGGT A^CCGGT
4002 ACCGGT
PshAI 1133 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4084 GACCAAGGTC
SmaI 1766 CCCGGG CCC^GGG
2668 CCCGGG
SspI 3853 AATATT AAT^ATT
4327 AATATT
XmaCI 1766 CCCGGG C^CCGGG
2668 CCCGGG
XmaI 1766 CCCGGG C^CCGGG
2668 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI Sse8387I StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I Van91I XcmI XhoII