################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 961 GAATTT [GA]^AATT[CT] AfaI 1051 GTAC GT^AC AflII 846 CTTAAG C^TTAAG AlwNI 651 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 961 GAATTT [GA]^AATT[CT] AspHI 682 GAGCTC G[AT]GC[AT]^C AvaI 624 CTCGAG C^[CT]CG[GA]G BanII 682 GAGCTC G[GA]GC[CT]^C BbvCI 1037 CCTCAGC CCTCAGC(-5/-2) BfrI 846 CTTAAG C^TTAAG BmyI 682 GAGCTC G[AGT]GC[ACT]^C BpmI 444 CTGGAG CTGGAG(16/14) BsaI 460 GAGACC GGTCTC(1/5) BseRI 537 GAGGAG GAGGAG(10/8) BsiHKAI 682 GAGCTC G[AT]GC[AT]^C BsmFI 1087 GGGAC GGGAC(10/14) BsoBI 624 CTCGAG C^[CT]CG[GA]G Bsp1286I 682 GAGCTC G[AGT]GC[ACT]^C BspHI 178 TCATGA T^CATGA BsrDI 874 CATTGC GCAATG(2/0) BstBI 391 TTCGAA TT^CGAA BstYI 350 AGATCC [GA]^GATC[CT] DraII 652 AGGCCCT [GA]G^G[ACGT]CC[CT] EarI 1005 GAAGAG CTCTTC(1/4) EcoO109I 652 AGGCCCT [GA]G^G[ACGT]CC[CT] HincII 275 GTTAAC GT[CT]^[GA]AC HindII 275 GTTAAC GT[CT]^[GA]AC HindIII 141 AAGCTT A^AGCTT HpaI 275 GTTAAC GTT^AAC HphI 1133 TCACC GGTGA(8/7) Ksp632I 1005 GAAGAG CTCTTC(1/4) MaeII 916 ACGT A^CGT MflI 350 AGATCC [GA]^GATC[CT] MslI 507 CACGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIV 904 GGAACC GG[ACGT]^[ACGT]CC NspV 391 TTCGAA TT^CGAA PacI 120 TTAATTAA TTAAT^TAA PaeR7I 624 CTCGAG C^TCGAG PshAI 195 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 178 TCATGA T^CATGA RsaI 1051 GTAC GT^AC SacI 682 GAGCTC GAGCT^C SfaNI 315 GATGC GCATC(5/9) SfuI 391 TTCGAA TT^CGAA SphI 283 GCATGC GCATG^C XhoI 624 CTCGAG C^TCGAG XhoII 350 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 781 CCGC CCGC(-3/-1) 272 GCGG AflIII 700 ACATGT A^C[GA][CT]GT 862 ACATGT AlwI 351 GATCC GGATC(4/5) 160 GGATC AseI 119 ATTAAT AT^TAAT 123 ATTAAT AsnI 119 ATTAAT AT^TAAT 123 ATTAAT BsmAI 460 GAGAC GTCTC(1/5) 870 GAGAC BspLU11I 700 ACATGT A^CATGT 862 ACATGT BspMI 173 GCAGGT ACCTGC(4/8) 813 GCAGGT Cac8I 283 GCATGC GC[ACGT]^[ACGT]GC 1042 GCTTGC Cfr13I 653 GGCCC G^G[ACGT]CC 778 GGGCC MwoI 516 GCCAAAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 771 GCTAGTTGGGC NcoI 1083 CCATGG C^CATGG 1158 CCATGG PshBI 119 ATTAAT AT^TAAT 123 ATTAAT Sau96I 653 GGCCC G^G[ACGT]CC 778 GGGCC TspRI 14 CACTG CA[GC]TG[ACGT][ACGT]^ 362 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AluI BbsI BbvI BfaI BpuAI BsaJI BssKI BstF5I BstNI DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NspI Sau3AI ScrFI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TthHB8I