##################################
# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BcnI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsiYI
BslI BsmBI BsmI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcsI 961 GAATTT [GA]^AATT[CT]
AfaI 1051 GTAC GT^AC
AflII 846 CTTAAG C^TTAAG
AlwNI 651 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 961 GAATTT [GA]^AATT[CT]
AspHI 682 GAGCTC G[AT]GC[AT]^C
AvaI 624 CTCGAG C^[CT]CG[GA]G
BanII 682 GAGCTC G[GA]GC[CT]^C
BbvCI 1037 CCTCAGC CCTCAGC(-5/-2)
BfrI 846 CTTAAG C^TTAAG
BmyI 682 GAGCTC G[AGT]GC[ACT]^C
BpmI 444 CTGGAG CTGGAG(16/14)
BsaI 460 GAGACC GGTCTC(1/5)
BseRI 537 GAGGAG GAGGAG(10/8)
BsiHKAI 682 GAGCTC G[AT]GC[AT]^C
BsmFI 1087 GGGAC GGGAC(10/14)
BsoBI 624 CTCGAG C^[CT]CG[GA]G
Bsp1286I 682 GAGCTC G[AGT]GC[ACT]^C
BspHI 178 TCATGA T^CATGA
BsrDI 874 CATTGC GCAATG(2/0)
BstBI 391 TTCGAA TT^CGAA
BstYI 350 AGATCC [GA]^GATC[CT]
DraII 652 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 1005 GAAGAG CTCTTC(1/4)
EcoO109I 652 AGGCCCT [GA]G^G[ACGT]CC[CT]
HincII 275 GTTAAC GT[CT]^[GA]AC
HindII 275 GTTAAC GT[CT]^[GA]AC
HindIII 141 AAGCTT A^AGCTT
HpaI 275 GTTAAC GTT^AAC
HphI 1133 TCACC GGTGA(8/7)
Ksp632I 1005 GAAGAG CTCTTC(1/4)
MaeII 916 ACGT A^CGT
MflI 350 AGATCC [GA]^GATC[CT]
MslI 507 CACGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV 904 GGAACC GG[ACGT]^[ACGT]CC
NspV 391 TTCGAA TT^CGAA
PacI 120 TTAATTAA TTAAT^TAA
PaeR7I 624 CTCGAG C^TCGAG
PshAI 195 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 178 TCATGA T^CATGA
RsaI 1051 GTAC GT^AC
SacI 682 GAGCTC GAGCT^C
SfaNI 315 GATGC GCATC(5/9)
SfuI 391 TTCGAA TT^CGAA
SphI 283 GCATGC GCATG^C
XhoI 624 CTCGAG C^TCGAG
XhoII 350 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 781 CCGC CCGC(-3/-1)
272 GCGG
AflIII 700 ACATGT A^C[GA][CT]GT
862 ACATGT
AlwI 351 GATCC GGATC(4/5)
160 GGATC
AseI 119 ATTAAT AT^TAAT
123 ATTAAT
AsnI 119 ATTAAT AT^TAAT
123 ATTAAT
BsmAI 460 GAGAC GTCTC(1/5)
870 GAGAC
BspLU11I 700 ACATGT A^CATGT
862 ACATGT
BspMI 173 GCAGGT ACCTGC(4/8)
813 GCAGGT
Cac8I 283 GCATGC GC[ACGT]^[ACGT]GC
1042 GCTTGC
Cfr13I 653 GGCCC G^G[ACGT]CC
778 GGGCC
MwoI 516 GCCAAAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
771 GCTAGTTGGGC
NcoI 1083 CCATGG C^CATGG
1158 CCATGG
PshBI 119 ATTAAT AT^TAAT
123 ATTAAT
Sau96I 653 GGCCC G^G[ACGT]CC
778 GGGCC
TspRI 14 CACTG CA[GC]TG[ACGT][ACGT]^
362 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI BbsI BbvI BfaI BpuAI
BsaJI BssKI BstF5I BstNI DdeI
DpnII DsaI EcoRII EcoT14I Fnu4HI
FokI HaeIII ItaI MaeI MaeIII
MboI MboII MnlI MseI MvaI
NdeII NlaIII NspI Sau3AI ScrFI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TthHB8I