##################################
# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI StuI
StyI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 527 GGTACC G^GTACC
AflIII 18 ACATGT A^C[GA][CT]GT
Asp718I 527 GGTACC G^GTACC
BalI 181 TGGCCA TGG^CCA
BamHI 620 GGATCC G^GATCC
BbvCI 43 CCTCAGC CCTCAGC(-5/-2)
BcnI 227 CCGGG CC^[GC]GG
BfaI 406 CTAG C^TAG
BmyI 73 GTGCCC G[AGT]GC[ACT]^C
BseRI 41 CTCCTC GAGGAG(10/8)
BsmFI 270 GGGAC GGGAC(10/14)
Bsp1286I 73 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 18 ACATGT A^CATGT
BsrFI 205 ACCGGC [GA]^CCGG[CT]
BsrI 108 ACTGG ACTGG(1/-1)
BstEII 286 GGTCACC G^GT[ACGT]ACC
BstPI 286 GGTCACC G^GT[ACGT]ACC
BstYI 620 GGATCC [GA]^GATC[CT]
CfoI 475 GCGC GCG^C
Cfr10I 205 ACCGGC [GA]^CCGG[CT]
DpnII 621 GATC ^GATC
DraII 235 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 17 GACATGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 181 TGGCCA [CT]^GGCC[GA]
EcoO109I 235 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 286 GGTCACC G^GT[ACGT]ACC
EcoRI 249 GAATTC G^AATTC
HaeII 474 GGCGCT [GA]GCGC^[CT]
HhaI 475 GCGC GCG^C
HinP1I 475 GCGC G^CGC
HinfI 481 GAATC G^A[ACGT]TC
KpnI 527 GGTACC GGTAC^C
MaeI 406 CTAG C^TAG
MaeII 388 ACGT A^CGT
MboI 621 GATC ^GATC
MflI 620 GGATCC [GA]^GATC[CT]
MluNI 181 TGGCCA TGG^CCA
MscI 181 TGGCCA TGG^CCA
MspA1I 162 CAGCGG C[AC]G^C[GT]G
NciI 227 CCGGG CC^[GC]GG
NdeII 621 GATC ^GATC
NspI 18 ACATGT [GA]CATG^[CT]
PpuMI 235 AGGTCCT [GA]G^G[AT]CC[CT]
Sau3AI 621 GATC ^GATC
SexAI 68 ACCTGGT A^CC[AT]GGT
SpeI 405 ACTAGT A^CTAGT
SspI 585 AATATT AAT^ATT
TfiI 481 GAATC G^A[AT]TC
TspRI 107 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 620 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 249 GAATTC [GA]^AATT[CT]
630 GAATTT
AfaI 97 GTAC GT^AC
528 GTAC
AluI 258 AGCT AG^CT
605 AGCT
AlwI 621 GATCC GGATC(4/5)
620 GGATC
ApoI 249 GAATTC [GA]^AATT[CT]
630 GAATTT
AvaII 236 GGTCC G^G[AT]CC
331 GGTCC
BanI 72 GGTGCC G^G[CT][GA]CC
527 GGTACC
BpmI 210 CTGGAG CTGGAG(16/14)
240 CTGGAG
BspMI 29 ACCTGC ACCTGC(4/8)
80 ACCTGC
BstF5I 61 CATCC GGATG(2/0)
493 CATCC
BstNI 69 CCTGG CC^[AT]GG
239 CCTGG
EarI 444 CTCTTC CTCTTC(1/4)
231 GAAGAG
EcoRII 69 CCTGG ^CC[AT]GG
239 CCTGG
FokI 61 CATCC GGATG(9/13)
493 CATCC
HapII 206 CCGG C^CGG
227 CCGG
HpaII 206 CCGG C^CGG
227 CCGG
Ksp632I 444 CTCTTC CTCTTC(1/4)
231 GAAGAG
MboII 231 GAAGA GAAGA(8/7)
445 TCTTC
MseI 377 TTAA T^TAA
612 TTAA
MslI 140 CACTAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
355 CATGCAGATG
MspI 206 CCGG C^CGG
227 CCGG
MvaI 69 CCTGG CC^[AT]GG
239 CCTGG
RsaI 97 GTAC GT^AC
528 GTAC
SfaNI 433 GATGC GCATC(5/9)
646 GATGC
Tru9I 377 TTAA T^TAA
612 TTAA
Tsp509I 250 AATT ^AATT
631 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI BbvI BsiYI BslI BssKI
Cfr13I DdeI Fnu4HI HaeIII HphI
ItaI MaeIII MnlI MwoI NlaIII
NlaIV Sau96I ScrFI TseI Tsp45I