##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
Acc65I AccII AclI AcsI AflII
AgeI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AviII AvrII BalI BamHI BbvCI
BciVI BcnI BfrI BlnI BlpI
Bpu1102I BsaI BsiEI BsiWI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HindIII HpaI KpnI
KspI MfeI MluI MluNI MscI
MunI MvnI NaeI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 152 GACGTC GACGT^C
AccI 474 GTCGAC GT^[AC][GT]AC
AccIII 1231 TCCGGA T^CCGGA
AflIII 759 ACGTGT A^C[GA][CT]GT
AhdI 1021 GACGGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 369 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 1021 GACGGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 43 GGCGCC GGCGC^C
BbrPI 758 CACGTG CAC^GTG
BbsI 663 GAAGAC GAAGAC(2/6)
BcgI 416 CGAGCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1183 TGATCA T^GATCA
BglI 910 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1612 CTGGAG CTGGAG(16/14)
BpuAI 663 GAAGAC GAAGAC(2/6)
BsaAI 758 CACGTG [CT]AC^GT[GA]
BsaBI 249 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1231 TCCGGA [AT]^CCGG[AT]
BseAI 1231 TCCGGA T^CCGGA
BsgI 1080 GTGCAG GTGCAG(16/14)
BsmBI 84 CGTCTC CGTCTC(1/5)
BsmI 575 GAATGC GAATGC(1/-1)
BspEI 1231 TCCGGA T^CCGGA
BspMI 962 ACCTGC ACCTGC(4/8)
BsrBI 603 GAGCGG CCGCTC(-3/-3)
BsrDI 1007 CATTGC GCAATG(2/0)
Cac8I 419 GCAGGC GC[ACGT]^[ACGT]GC
Eam1105I 1021 GACGGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 861 GAAGAG CTCTTC(1/4)
FbaI 1183 TGATCA T^GATCA
HaeII 43 GGCGCC [GA]GCGC^[CT]
HapII 1232 CCGG C^CGG
HgaI 9 GCGTC GACGC(5/10)
HpaII 1232 CCGG C^CGG
KasI 43 GGCGCC G^GCGCC
Ksp632I 861 GAAGAG CTCTTC(1/4)
MamI 249 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1231 TCCGGA T^CCGGA
MspI 1232 CCGG C^CGG
NarI 43 GGCGCC GG^CGCC
NspI 1517 GCATGT [GA]CATG^[CT]
PleI 1455 GAGTC GAGTC(4/5)
PmaCI 758 CACGTG CAC^GTG
PmlI 758 CACGTG CAC^GTG
SalI 474 GTCGAC G^TCGAC
SfoI 43 GGCGCC GGC^GCC
XbaI 1143 TCTAGA T^CTAGA
XcmI 4 CCATGGCGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 842 GTAC GT^AC
1635 GTAC
AspHI 624 GAGCTC G[AT]GC[AT]^C
1044 GAGCTC
BanI 43 GGCGCC G^G[CT][GA]CC
225 GGTGCC
BfaI 900 CTAG C^TAG
1144 CTAG
BglII 1087 AGATCT A^GATCT
1552 AGATCT
BseRI 1168 GAGGAG GAGGAG(10/8)
1267 GAGGAG
BsiHKAI 624 GAGCTC G[AT]GC[AT]^C
1044 GAGCTC
BsmAI 85 GTCTC GTCTC(1/5)
110 GTCTC
BstYI 1087 AGATCT [GA]^GATC[CT]
1552 AGATCT
CfoI 44 GCGC GCG^C
1291 GCGC
HhaI 44 GCGC GCG^C
1291 GCGC
HinP1I 44 GCGC G^CGC
1291 GCGC
MaeI 900 CTAG C^TAG
1144 CTAG
MaeII 153 ACGT A^CGT
759 ACGT
MflI 1087 AGATCT [GA]^GATC[CT]
1552 AGATCT
NcoI 4 CCATGG C^CATGG
1381 CCATGG
PpuMI 18 GGGACCC [GA]G^G[AT]CC[CT]
983 GGGACCT
PstI 834 CTGCAG CTGCA^G
964 CTGCAG
PvuII 369 CAGCTG CAG^CTG
542 CAGCTG
RsaI 842 GTAC GT^AC
1635 GTAC
SacI 624 GAGCTC GAGCT^C
1044 GAGCTC
SmlI 96 CTTGAG C^T[CT][GA]AG
105 CTCAAG
XhoII 1087 AGATCT [GA]^GATC[CT]
1552 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcyI AluI AvaII BanII
BbvI BmyI BsaHI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII Hin1I
HincII HindII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I