################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## Acc65I AccII AclI AcsI AflII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BalI BamHI BbvCI BciVI BcnI BfrI BlnI BlpI Bpu1102I BsaI BsiEI BsiWI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HindIII HpaI KpnI KspI MfeI MluI MluNI MscI MunI MvnI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 152 GACGTC GACGT^C AccI 474 GTCGAC GT^[AC][GT]AC AccIII 1231 TCCGGA T^CCGGA AflIII 759 ACGTGT A^C[GA][CT]GT AhdI 1021 GACGGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 369 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 1021 GACGGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 43 GGCGCC GGCGC^C BbrPI 758 CACGTG CAC^GTG BbsI 663 GAAGAC GAAGAC(2/6) BcgI 416 CGAGCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1183 TGATCA T^GATCA BglI 910 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1612 CTGGAG CTGGAG(16/14) BpuAI 663 GAAGAC GAAGAC(2/6) BsaAI 758 CACGTG [CT]AC^GT[GA] BsaBI 249 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1231 TCCGGA [AT]^CCGG[AT] BseAI 1231 TCCGGA T^CCGGA BsgI 1080 GTGCAG GTGCAG(16/14) BsmBI 84 CGTCTC CGTCTC(1/5) BsmI 575 GAATGC GAATGC(1/-1) BspEI 1231 TCCGGA T^CCGGA BspMI 962 ACCTGC ACCTGC(4/8) BsrBI 603 GAGCGG CCGCTC(-3/-3) BsrDI 1007 CATTGC GCAATG(2/0) Cac8I 419 GCAGGC GC[ACGT]^[ACGT]GC Eam1105I 1021 GACGGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 861 GAAGAG CTCTTC(1/4) FbaI 1183 TGATCA T^GATCA HaeII 43 GGCGCC [GA]GCGC^[CT] HapII 1232 CCGG C^CGG HgaI 9 GCGTC GACGC(5/10) HpaII 1232 CCGG C^CGG KasI 43 GGCGCC G^GCGCC Ksp632I 861 GAAGAG CTCTTC(1/4) MamI 249 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1231 TCCGGA T^CCGGA MspI 1232 CCGG C^CGG NarI 43 GGCGCC GG^CGCC NspI 1517 GCATGT [GA]CATG^[CT] PleI 1455 GAGTC GAGTC(4/5) PmaCI 758 CACGTG CAC^GTG PmlI 758 CACGTG CAC^GTG SalI 474 GTCGAC G^TCGAC SfoI 43 GGCGCC GGC^GCC XbaI 1143 TCTAGA T^CTAGA XcmI 4 CCATGGCGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 842 GTAC GT^AC 1635 GTAC AspHI 624 GAGCTC G[AT]GC[AT]^C 1044 GAGCTC BanI 43 GGCGCC G^G[CT][GA]CC 225 GGTGCC BfaI 900 CTAG C^TAG 1144 CTAG BglII 1087 AGATCT A^GATCT 1552 AGATCT BseRI 1168 GAGGAG GAGGAG(10/8) 1267 GAGGAG BsiHKAI 624 GAGCTC G[AT]GC[AT]^C 1044 GAGCTC BsmAI 85 GTCTC GTCTC(1/5) 110 GTCTC BstYI 1087 AGATCT [GA]^GATC[CT] 1552 AGATCT CfoI 44 GCGC GCG^C 1291 GCGC HhaI 44 GCGC GCG^C 1291 GCGC HinP1I 44 GCGC G^CGC 1291 GCGC MaeI 900 CTAG C^TAG 1144 CTAG MaeII 153 ACGT A^CGT 759 ACGT MflI 1087 AGATCT [GA]^GATC[CT] 1552 AGATCT NcoI 4 CCATGG C^CATGG 1381 CCATGG PpuMI 18 GGGACCC [GA]G^G[AT]CC[CT] 983 GGGACCT PstI 834 CTGCAG CTGCA^G 964 CTGCAG PvuII 369 CAGCTG CAG^CTG 542 CAGCTG RsaI 842 GTAC GT^AC 1635 GTAC SacI 624 GAGCTC GAGCT^C 1044 GAGCTC SmlI 96 CTTGAG C^T[CT][GA]AG 105 CTCAAG XhoII 1087 AGATCT [GA]^GATC[CT] 1552 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AcyI AluI AvaII BanII BbvI BmyI BsaHI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII Hin1I HincII HindII HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I