##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiWI BsmAI BsmBI
BsmFI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoNI
EcoO65I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NarI NcoI NheI
NotI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AflII 176 CTTAAG C^TTAAG
Alw44I 260 GTGCAC G^TGCAC
AocI 362 CCTGAGG CC^T[ACGT]AGG
ApaI 70 GGGCCC GGGCC^C
ApaLI 260 GTGCAC G^TGCAC
AspHI 260 GTGCAC G[AT]GC[AT]^C
BalI 834 TGGCCA TGG^CCA
BbsI 891 GTCTTC GAAGAC(2/6)
BciVI 878 GGATAC GTATCC(6/5)
BfaI 16 CTAG C^TAG
BfrI 176 CTTAAG C^TTAAG
BglII 1060 AGATCT A^GATCT
BpuAI 891 GTCTTC GAAGAC(2/6)
BsiEI 151 CGGCCG CG[GA][CT]^CG
BsiHKAI 260 GTGCAC G[AT]GC[AT]^C
BspHI 1015 TCATGA T^CATGA
BsrDI 861 GCAATG GCAATG(2/0)
BsrFI 159 GCCGGC [GA]^CCGG[CT]
BstAPI 286 GCAAATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 362 CCTGAGG CC^T[ACGT]AGG
CfoI 341 GCGC GCG^C
Cfr10I 159 GCCGGC [GA]^CCGG[CT]
DraII 69 GGGGCCC [GA]G^G[ACGT]CC[CT]
EagI 151 CGGCCG C^GGCCG
EarI 281 GAAGAG CTCTTC(1/4)
EclXI 151 CGGCCG C^GGCCG
Eco52I 151 CGGCCG C^GGCCG
Eco81I 362 CCTGAGG CC^T[ACGT]AGG
EcoO109I 69 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 304 GAATTC G^AATTC
EcoT14I 908 CCAAGG C^C[AT][AT]GG
FauI 167 CCCGC CCCGC(4/6)
HgaI 217 GACGC GACGC(5/10)
HhaI 341 GCGC GCG^C
HinP1I 341 GCGC G^CGC
HindIII 541 AAGCTT A^AGCTT
Ksp632I 281 GAAGAG CTCTTC(1/4)
KspI 145 CCGCGG CCGC^GG
MaeI 16 CTAG C^TAG
MluNI 834 TGGCCA TGG^CCA
MscI 834 TGGCCA TGG^CCA
NaeI 159 GCCGGC GCC^GGC
NdeI 633 CATATG CA^TATG
NgoMIV 159 GCCGGC G^CCGGC
NruI 184 TCGCGA TCG^CGA
PstI 775 CTGCAG CTGCA^G
PvuII 296 CAGCTG CAG^CTG
RcaI 1015 TCATGA T^CATGA
SacII 145 CCGCGG CCGC^GG
SapI 281 GAAGAGC GCTCTTC(1/4)
ScaI 872 AGTACT AGT^ACT
SfiI 143 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1166 CCCGGG CCC^GGG
SmlI 176 CTTAAG C^T[CT][GA]AG
StyI 908 CCAAGG C^C[AT][AT]GG
Tsp45I 680 GTGAC ^GT[GC]AC
XmaCI 1166 CCCGGG C^CCGGG
XmaI 1166 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 296 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
819 CAGTCGCTG
AvaI 124 CTCGGG C^[CT]CG[GA]G
1166 CCCGGG
BglI 72 GCCCACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
144 GCCGCGGCGGC
BsgI 934 CTGCAC GTGCAG(16/14)
293 GTGCAG
BsmI 639 GAATGC GAATGC(1/-1)
1114 GAATGC
BsoBI 124 CTCGGG C^[CT]CG[GA]G
1166 CCCGGG
BstYI 1060 AGATCT [GA]^GATC[CT]
1250 AGATCC
Cac8I 159 GCCGGC GC[ACGT]^[ACGT]GC
773 GCCTGC
DsaI 74 CCACGG C^C[GA][CT]GG
145 CCGCGG
EcoRV 346 GATATC GAT^ATC
1205 GATATC
HphI 679 GGTGA GGTGA(8/7)
695 GGTGA
MaeII 274 ACGT A^CGT
976 ACGT
MflI 1060 AGATCT [GA]^GATC[CT]
1250 AGATCC
MslI 263 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
371 CATGGGTATG
PleI 971 GACTC GAGTC(4/5)
851 GAGTC
SfaNI 604 GATGC GCATC(5/9)
163 GCATC
SspI 1093 AATATT AAT^ATT
1134 AATATT
TspRI 956 CAGTG CA[GC]TG[ACGT][ACGT]^
985 CAGTG
XhoII 1060 AGATCT [GA]^GATC[CT]
1250 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfcI TaqI TfiI Tru9I
TseI Tsp509I TthHB8I