##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflIII      AgeI        AhdI        Aor51HI     
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaII       AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BclI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiWI       BsmAI       BsmBI       
BsmFI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    EcoNI       
EcoO65I     EcoT22I     FbaI        FseI        FspI        
HaeII       Hin1I       HincII      HindII      HpaI        
KasI        KpnI        MamI        MfeI        MluI        
MroI        MunI        NarI        NcoI        NheI        
NotI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SalI        
SbfI        SexAI       SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AflII         176  CTTAAG               C^TTAAG
Alw44I        260  GTGCAC               G^TGCAC
AocI          362  CCTGAGG              CC^T[ACGT]AGG
ApaI           70  GGGCCC               GGGCC^C
ApaLI         260  GTGCAC               G^TGCAC
AspHI         260  GTGCAC               G[AT]GC[AT]^C
BalI          834  TGGCCA               TGG^CCA
BbsI          891  GTCTTC               GAAGAC(2/6)
BciVI         878  GGATAC               GTATCC(6/5)
BfaI           16  CTAG                 C^TAG
BfrI          176  CTTAAG               C^TTAAG
BglII        1060  AGATCT               A^GATCT
BpuAI         891  GTCTTC               GAAGAC(2/6)
BsiEI         151  CGGCCG               CG[GA][CT]^CG
BsiHKAI       260  GTGCAC               G[AT]GC[AT]^C
BspHI        1015  TCATGA               T^CATGA
BsrDI         861  GCAATG               GCAATG(2/0)
BsrFI         159  GCCGGC               [GA]^CCGG[CT]
BstAPI        286  GCAAATTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        362  CCTGAGG              CC^T[ACGT]AGG
CfoI          341  GCGC                 GCG^C
Cfr10I        159  GCCGGC               [GA]^CCGG[CT]
DraII          69  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EagI          151  CGGCCG               C^GGCCG
EarI          281  GAAGAG               CTCTTC(1/4)
EclXI         151  CGGCCG               C^GGCCG
Eco52I        151  CGGCCG               C^GGCCG
Eco81I        362  CCTGAGG              CC^T[ACGT]AGG
EcoO109I       69  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         304  GAATTC               G^AATTC
EcoT14I       908  CCAAGG               C^C[AT][AT]GG
FauI          167  CCCGC                CCCGC(4/6)
HgaI          217  GACGC                GACGC(5/10)
HhaI          341  GCGC                 GCG^C
HinP1I        341  GCGC                 G^CGC
HindIII       541  AAGCTT               A^AGCTT
Ksp632I       281  GAAGAG               CTCTTC(1/4)
KspI          145  CCGCGG               CCGC^GG
MaeI           16  CTAG                 C^TAG
MluNI         834  TGGCCA               TGG^CCA
MscI          834  TGGCCA               TGG^CCA
NaeI          159  GCCGGC               GCC^GGC
NdeI          633  CATATG               CA^TATG
NgoMIV        159  GCCGGC               G^CCGGC
NruI          184  TCGCGA               TCG^CGA
PstI          775  CTGCAG               CTGCA^G
PvuII         296  CAGCTG               CAG^CTG
RcaI         1015  TCATGA               T^CATGA
SacII         145  CCGCGG               CCGC^GG
SapI          281  GAAGAGC              GCTCTTC(1/4)
ScaI          872  AGTACT               AGT^ACT
SfiI          143  GGCCGCGGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1166  CCCGGG               CCC^GGG
SmlI          176  CTTAAG               C^T[CT][GA]AG
StyI          908  CCAAGG               C^C[AT][AT]GG
Tsp45I        680  GTGAC                ^GT[GC]AC
XmaCI        1166  CCCGGG               C^CCGGG
XmaI         1166  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwNI         296  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              819  CAGTCGCTG           
AvaI          124  CTCGGG               C^[CT]CG[GA]G
             1166  CCCGGG              
BglI           72  GCCCACGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              144  GCCGCGGCGGC         
BsgI          934  CTGCAC               GTGCAG(16/14)
              293  GTGCAG              
BsmI          639  GAATGC               GAATGC(1/-1)
             1114  GAATGC              
BsoBI         124  CTCGGG               C^[CT]CG[GA]G
             1166  CCCGGG              
BstYI        1060  AGATCT               [GA]^GATC[CT]
             1250  AGATCC              
Cac8I         159  GCCGGC               GC[ACGT]^[ACGT]GC
              773  GCCTGC              
DsaI           74  CCACGG               C^C[GA][CT]GG
              145  CCGCGG              
EcoRV         346  GATATC               GAT^ATC
             1205  GATATC              
HphI          679  GGTGA                GGTGA(8/7)
              695  GGTGA               
MaeII         274  ACGT                 A^CGT
              976  ACGT                
MflI         1060  AGATCT               [GA]^GATC[CT]
             1250  AGATCC              
MslI          263  CACATGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              371  CATGGGTATG          
PleI          971  GACTC                GAGTC(4/5)
              851  GAGTC               
SfaNI         604  GATGC                GCATC(5/9)
              163  GCATC               
SspI         1093  AATATT               AAT^ATT
             1134  AATATT              
TspRI         956  CAGTG                CA[GC]TG[ACGT][ACGT]^
              985  CAGTG               
XhoII        1060  AGATCT               [GA]^GATC[CT]
             1250  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       Cfr13I      DdeI        DpnII       EaeI        
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       ItaI        MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     TthHB8I