################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BclI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiWI BsmAI BsmBI BsmFI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoO65I EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NcoI NheI NotI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SbfI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AflII 176 CTTAAG C^TTAAG Alw44I 260 GTGCAC G^TGCAC AocI 362 CCTGAGG CC^T[ACGT]AGG ApaI 70 GGGCCC GGGCC^C ApaLI 260 GTGCAC G^TGCAC AspHI 260 GTGCAC G[AT]GC[AT]^C BalI 834 TGGCCA TGG^CCA BbsI 891 GTCTTC GAAGAC(2/6) BciVI 878 GGATAC GTATCC(6/5) BfaI 16 CTAG C^TAG BfrI 176 CTTAAG C^TTAAG BglII 1060 AGATCT A^GATCT BpuAI 891 GTCTTC GAAGAC(2/6) BsiEI 151 CGGCCG CG[GA][CT]^CG BsiHKAI 260 GTGCAC G[AT]GC[AT]^C BspHI 1015 TCATGA T^CATGA BsrDI 861 GCAATG GCAATG(2/0) BsrFI 159 GCCGGC [GA]^CCGG[CT] BstAPI 286 GCAAATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 362 CCTGAGG CC^T[ACGT]AGG CfoI 341 GCGC GCG^C Cfr10I 159 GCCGGC [GA]^CCGG[CT] DraII 69 GGGGCCC [GA]G^G[ACGT]CC[CT] EagI 151 CGGCCG C^GGCCG EarI 281 GAAGAG CTCTTC(1/4) EclXI 151 CGGCCG C^GGCCG Eco52I 151 CGGCCG C^GGCCG Eco81I 362 CCTGAGG CC^T[ACGT]AGG EcoO109I 69 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoRI 304 GAATTC G^AATTC EcoT14I 908 CCAAGG C^C[AT][AT]GG FauI 167 CCCGC CCCGC(4/6) HgaI 217 GACGC GACGC(5/10) HhaI 341 GCGC GCG^C HinP1I 341 GCGC G^CGC HindIII 541 AAGCTT A^AGCTT Ksp632I 281 GAAGAG CTCTTC(1/4) KspI 145 CCGCGG CCGC^GG MaeI 16 CTAG C^TAG MluNI 834 TGGCCA TGG^CCA MscI 834 TGGCCA TGG^CCA NaeI 159 GCCGGC GCC^GGC NdeI 633 CATATG CA^TATG NgoMIV 159 GCCGGC G^CCGGC NruI 184 TCGCGA TCG^CGA PstI 775 CTGCAG CTGCA^G PvuII 296 CAGCTG CAG^CTG RcaI 1015 TCATGA T^CATGA SacII 145 CCGCGG CCGC^GG SapI 281 GAAGAGC GCTCTTC(1/4) ScaI 872 AGTACT AGT^ACT SfiI 143 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1166 CCCGGG CCC^GGG SmlI 176 CTTAAG C^T[CT][GA]AG StyI 908 CCAAGG C^C[AT][AT]GG Tsp45I 680 GTGAC ^GT[GC]AC XmaCI 1166 CCCGGG C^CCGGG XmaI 1166 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 296 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 819 CAGTCGCTG AvaI 124 CTCGGG C^[CT]CG[GA]G 1166 CCCGGG BglI 72 GCCCACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 144 GCCGCGGCGGC BsgI 934 CTGCAC GTGCAG(16/14) 293 GTGCAG BsmI 639 GAATGC GAATGC(1/-1) 1114 GAATGC BsoBI 124 CTCGGG C^[CT]CG[GA]G 1166 CCCGGG BstYI 1060 AGATCT [GA]^GATC[CT] 1250 AGATCC Cac8I 159 GCCGGC GC[ACGT]^[ACGT]GC 773 GCCTGC DsaI 74 CCACGG C^C[GA][CT]GG 145 CCGCGG EcoRV 346 GATATC GAT^ATC 1205 GATATC HphI 679 GGTGA GGTGA(8/7) 695 GGTGA MaeII 274 ACGT A^CGT 976 ACGT MflI 1060 AGATCT [GA]^GATC[CT] 1250 AGATCC MslI 263 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 371 CATGGGTATG PleI 971 GACTC GAGTC(4/5) 851 GAGTC SfaNI 604 GATGC GCATC(5/9) 163 GCATC SspI 1093 AATATT AAT^ATT 1134 AATATT TspRI 956 CAGTG CA[GC]TG[ACGT][ACGT]^ 985 CAGTG XhoII 1060 AGATCT [GA]^GATC[CT] 1250 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI TaqI TfiI Tru9I TseI Tsp509I TthHB8I