##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BamHI BbrPI BbvCI BciVI BfrI
BglII BsaAI BsaBI BseAI BseRI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeII
MamI MfeI MflI MluI MroI
MslI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AocI 888 CCTCAGG CC^T[ACGT]AGG
AvrII 613 CCTAGG C^CTAGG
BbsI 692 GTCTTC GAAGAC(2/6)
BcgI 339 GCATTACGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 174 TGATCA T^GATCA
BglI 16 GCCCGTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 613 CCTAGG C^CTAGG
BpuAI 692 GTCTTC GAAGAC(2/6)
BsaWI 435 ACCGGA [AT]^CCGG[AT]
BsgI 731 GTGCAG GTGCAG(16/14)
BspMI 77 GCAGGT ACCTGC(4/8)
BsrDI 195 CATTGC GCAATG(2/0)
BsrFI 123 GCCGGT [GA]^CCGG[CT]
BstXI 798 CCAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 888 CCTCAGG CC^T[ACGT]AGG
Cfr10I 123 GCCGGT [GA]^CCGG[CT]
DraII 884 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 624 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 677 GACCTTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 131 CCATGG C^C[GA][CT]GG
Eco81I 888 CCTCAGG CC^T[ACGT]AGG
EcoNI 805 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 884 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 212 GCGGG CCCGC(4/6)
FbaI 174 TGATCA T^GATCA
HgaI 110 GCGTC GACGC(5/10)
MseI 950 TTAA T^TAA
NcoI 131 CCATGG C^CATGG
PpuMI 884 AGGACCT [GA]G^G[AT]CC[CT]
SapI 773 GAAGAGC GCTCTTC(1/4)
SmaI 539 CCCGGG CCC^GGG
TfiI 570 GAATC G^A[AT]TC
Tru9I 950 TTAA T^TAA
TspRI 718 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 539 CCCGGG C^CCGGG
XmaI 539 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 13 GGCGCC G[GA]^CG[CT]C
146 GGCGCC
AfaI 27 GTAC GT^AC
802 GTAC
AvaI 405 CTCGGG C^[CT]CG[GA]G
539 CCCGGG
AvaII 438 GGACC G^G[AT]CC
885 GGACC
BalI 576 TGGCCA TGG^CCA
631 TGGCCA
BanI 13 GGCGCC G^G[CT][GA]CC
146 GGCGCC
BanII 263 GAGCTC G[GA]GC[CT]^C
402 GAGCTC
BbeI 13 GGCGCC GGCGC^C
146 GGCGCC
BcnI 539 CCCGG CC^[GC]GG
540 CCGGG
BlpI 284 GCTCAGC GC^T[ACGT]AGC
563 GCTGAGC
BpmI 399 CTGGAG CTGGAG(16/14)
720 CTGGAG
Bpu1102I 284 GCTCAGC GC^T[ACGT]AGC
563 GCTGAGC
BsaHI 13 GGCGCC G[GA]^CG[CT]C
146 GGCGCC
BsmFI 557 GGGAC GGGAC(10/14)
97 GTCCC
BsoBI 405 CTCGGG C^[CT]CG[GA]G
539 CCCGGG
BssSI 870 CACGAG CACGAG(-5/-1)
87 CTCGTG
BstF5I 277 GGATG GGATG(2/0)
513 GGATG
CelII 284 GCTCAGC GC^T[ACGT]AGC
563 GCTGAGC
DpnII 175 GATC ^GATC
229 GATC
EarI 203 GAAGAG CTCTTC(1/4)
773 GAAGAG
FokI 277 GGATG GGATG(9/13)
513 GGATG
Hin1I 13 GGCGCC G[GA]^CG[CT]C
146 GGCGCC
HphI 782 GGTGA GGTGA(8/7)
177 TCACC
KasI 13 GGCGCC G^GCGCC
146 GGCGCC
Ksp632I 203 GAAGAG CTCTTC(1/4)
773 GAAGAG
MaeIII 675 GTGAC ^GT[ACGT]AC
783 GTGAC
MboI 175 GATC ^GATC
229 GATC
MluNI 576 TGGCCA TGG^CCA
631 TGGCCA
MscI 576 TGGCCA TGG^CCA
631 TGGCCA
NarI 13 GGCGCC GG^CGCC
146 GGCGCC
NciI 539 CCCGG CC^[GC]GG
540 CCGGG
NdeII 175 GATC ^GATC
229 GATC
NlaIV 13 GGCGCC GG[ACGT]^[ACGT]CC
146 GGCGCC
PstI 158 CTGCAG CTGCA^G
791 CTGCAG
PvuII 561 CAGCTG CAG^CTG
847 CAGCTG
RsaI 27 GTAC GT^AC
802 GTAC
SacI 263 GAGCTC GAGCT^C
402 GAGCTC
Sau3AI 175 GATC ^GATC
229 GATC
SfaNI 514 GATGC GCATC(5/9)
448 GCATC
SfoI 13 GGCGCC GGC^GCC
146 GGCGCC
Tsp45I 675 GTGAC ^GT[GC]AC
783 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwNI AspHI BbvI
BfaI BmyI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DdeI EaeI EcoRII EcoT14I
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MboII MnlI MspA1I MspI MvaI
MwoI NlaIII PleI Sau96I ScrFI
SfcI SmlI StyI TaqI TseI
Tsp509I TthHB8I