################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvaII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MluI MluNI MscI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Van91I XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AfaI 465 GTAC GT^AC AflII 373 CTTAAG C^TTAAG Asp700I 412 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 118 GAGCTC G[AT]GC[AT]^C AspI 127 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 842 CCCGGG C^[CT]CG[GA]G AviII 142 TGCGCA TGC^GCA BanII 118 GAGCTC G[GA]GC[CT]^C BciVI 270 GTATCC GTATCC(6/5) BclI 687 TGATCA T^GATCA BfrI 373 CTTAAG C^TTAAG BglI 757 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 118 GAGCTC G[AGT]GC[ACT]^C BsiHKAI 118 GAGCTC G[AT]GC[AT]^C BsmFI 67 GGGAC GGGAC(10/14) BsoBI 842 CCCGGG C^[CT]CG[GA]G Bsp1286I 118 GAGCTC G[AGT]GC[ACT]^C CfoI 143 GCGC GCG^C DraII 764 AGGCCCT [GA]G^G[ACGT]CC[CT] EarI 814 GAAGAG CTCTTC(1/4) EcoNI 443 CCTTGTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 764 AGGCCCT [GA]G^G[ACGT]CC[CT] FbaI 687 TGATCA T^GATCA FspI 142 TGCGCA TGC^GCA HhaI 143 GCGC GCG^C HinP1I 143 GCGC G^CGC HphI 752 TCACC GGTGA(8/7) Ksp632I 814 GAAGAG CTCTTC(1/4) MaeIII 855 GTTAC ^GT[ACGT]AC MfeI 592 CAATTG C^AATTG MslI 753 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 709 CAGCTG C[AC]G^C[GT]G MunI 592 CAATTG C^AATTG NdeI 260 CATATG CA^TATG NlaIV 27 GGCTCC GG[ACGT]^[ACGT]CC PflFI 127 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 796 GAGTC GAGTC(4/5) PstI 560 CTGCAG CTGCA^G PvuII 709 CAGCTG CAG^CTG RsaI 465 GTAC GT^AC SacI 118 GAGCTC GAGCT^C SapI 814 GAAGAGC GCTCTTC(1/4) SmaI 842 CCCGGG CCC^GGG SphI 621 GCATGC GCATG^C Tsp509I 593 AATT ^AATT TspRI 297 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 127 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 61 CCATCGGGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 842 CCCGGG C^CCGGG XmaI 842 CCCGGG C^CCGGG XmnI 412 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccIII 108 TCCGGA T^CCGGA 339 TCCGGA AlwI 489 GATCC GGATC(4/5) 723 GGATC BcnI 842 CCCGG CC^[GC]GG 843 CCGGG BsaI 19 GAGACC GGTCTC(1/5) 171 GGTCTC BsaWI 108 TCCGGA [AT]^CCGG[AT] 339 TCCGGA BseAI 108 TCCGGA T^CCGGA 339 TCCGGA BseRI 112 GAGGAG GAGGAG(10/8) 115 GAGGAG BsmBI 254 CGTCTC CGTCTC(1/5) 306 GAGACG BspEI 108 TCCGGA T^CCGGA 339 TCCGGA BspMI 145 GCAGGT ACCTGC(4/8) 657 GCAGGT BstNI 88 CCAGG CC^[AT]GG 402 CCTGG BstYI 488 AGATCC [GA]^GATC[CT] 723 GGATCT Cfr13I 765 GGCCC G^G[ACGT]CC 840 GGCCC DdeI 191 CTCAG C^T[ACGT]AG 304 CTGAG EcoRII 88 CCAGG ^CC[AT]GG 402 CCTGG FauI 51 CCCGC CCCGC(4/6) 92 GCGGG HaeIII 765 GGCC GG^CC 840 GGCC MflI 488 AGATCC [GA]^GATC[CT] 723 GGATCT MroI 108 TCCGGA T^CCGGA 339 TCCGGA MseI 293 TTAA T^TAA 374 TTAA MvaI 88 CCAGG CC^[AT]GG 402 CCTGG MwoI 757 GCCATGCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 841 GCCCGGGCAGC NciI 842 CCCGG CC^[GC]GG 843 CCGGG NspI 602 ACATGC [GA]CATG^[CT] 621 GCATGC Sau96I 765 GGCCC G^G[ACGT]CC 840 GGCCC SfcI 32 CTATAG C^T[GA][CT]AG 560 CTGCAG SmlI 121 CTCAAG C^T[CT][GA]AG 373 CTTAAG Tru9I 293 TTAA T^TAA 374 TTAA XhoII 488 AGATCC [GA]^GATC[CT] 723 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AcyI AluI BbvI BfaI BsaHI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I Cac8I DpnII Fnu4HI FokI HapII HgaI Hin1I HinfI HpaII ItaI MaeI MboI MboII MnlI MspI NdeII NlaIII Sau3AI ScrFI SfaNI TaqI TfiI TseI TthHB8I XbaI