##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AvaII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FseI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MluI MluNI
MscI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI Tsp45I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AfaI 465 GTAC GT^AC
AflII 373 CTTAAG C^TTAAG
Asp700I 412 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 118 GAGCTC G[AT]GC[AT]^C
AspI 127 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 842 CCCGGG C^[CT]CG[GA]G
AviII 142 TGCGCA TGC^GCA
BanII 118 GAGCTC G[GA]GC[CT]^C
BciVI 270 GTATCC GTATCC(6/5)
BclI 687 TGATCA T^GATCA
BfrI 373 CTTAAG C^TTAAG
BglI 757 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 118 GAGCTC G[AGT]GC[ACT]^C
BsiHKAI 118 GAGCTC G[AT]GC[AT]^C
BsmFI 67 GGGAC GGGAC(10/14)
BsoBI 842 CCCGGG C^[CT]CG[GA]G
Bsp1286I 118 GAGCTC G[AGT]GC[ACT]^C
CfoI 143 GCGC GCG^C
DraII 764 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 814 GAAGAG CTCTTC(1/4)
EcoNI 443 CCTTGTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 764 AGGCCCT [GA]G^G[ACGT]CC[CT]
FbaI 687 TGATCA T^GATCA
FspI 142 TGCGCA TGC^GCA
HhaI 143 GCGC GCG^C
HinP1I 143 GCGC G^CGC
HphI 752 TCACC GGTGA(8/7)
Ksp632I 814 GAAGAG CTCTTC(1/4)
MaeIII 855 GTTAC ^GT[ACGT]AC
MfeI 592 CAATTG C^AATTG
MslI 753 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 709 CAGCTG C[AC]G^C[GT]G
MunI 592 CAATTG C^AATTG
NdeI 260 CATATG CA^TATG
NlaIV 27 GGCTCC GG[ACGT]^[ACGT]CC
PflFI 127 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 796 GAGTC GAGTC(4/5)
PstI 560 CTGCAG CTGCA^G
PvuII 709 CAGCTG CAG^CTG
RsaI 465 GTAC GT^AC
SacI 118 GAGCTC GAGCT^C
SapI 814 GAAGAGC GCTCTTC(1/4)
SmaI 842 CCCGGG CCC^GGG
SphI 621 GCATGC GCATG^C
Tsp509I 593 AATT ^AATT
TspRI 297 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 127 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 61 CCATCGGGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 842 CCCGGG C^CCGGG
XmaI 842 CCCGGG C^CCGGG
XmnI 412 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 108 TCCGGA T^CCGGA
339 TCCGGA
AlwI 489 GATCC GGATC(4/5)
723 GGATC
BcnI 842 CCCGG CC^[GC]GG
843 CCGGG
BsaI 19 GAGACC GGTCTC(1/5)
171 GGTCTC
BsaWI 108 TCCGGA [AT]^CCGG[AT]
339 TCCGGA
BseAI 108 TCCGGA T^CCGGA
339 TCCGGA
BseRI 112 GAGGAG GAGGAG(10/8)
115 GAGGAG
BsmBI 254 CGTCTC CGTCTC(1/5)
306 GAGACG
BspEI 108 TCCGGA T^CCGGA
339 TCCGGA
BspMI 145 GCAGGT ACCTGC(4/8)
657 GCAGGT
BstNI 88 CCAGG CC^[AT]GG
402 CCTGG
BstYI 488 AGATCC [GA]^GATC[CT]
723 GGATCT
Cfr13I 765 GGCCC G^G[ACGT]CC
840 GGCCC
DdeI 191 CTCAG C^T[ACGT]AG
304 CTGAG
EcoRII 88 CCAGG ^CC[AT]GG
402 CCTGG
FauI 51 CCCGC CCCGC(4/6)
92 GCGGG
HaeIII 765 GGCC GG^CC
840 GGCC
MflI 488 AGATCC [GA]^GATC[CT]
723 GGATCT
MroI 108 TCCGGA T^CCGGA
339 TCCGGA
MseI 293 TTAA T^TAA
374 TTAA
MvaI 88 CCAGG CC^[AT]GG
402 CCTGG
MwoI 757 GCCATGCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
841 GCCCGGGCAGC
NciI 842 CCCGG CC^[GC]GG
843 CCGGG
NspI 602 ACATGC [GA]CATG^[CT]
621 GCATGC
Sau96I 765 GGCCC G^G[ACGT]CC
840 GGCCC
SfcI 32 CTATAG C^T[GA][CT]AG
560 CTGCAG
SmlI 121 CTCAAG C^T[CT][GA]AG
373 CTTAAG
Tru9I 293 TTAA T^TAA
374 TTAA
XhoII 488 AGATCC [GA]^GATC[CT]
723 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcyI AluI BbvI BfaI
BsaHI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I Cac8I DpnII
Fnu4HI FokI HapII HgaI Hin1I
HinfI HpaII ItaI MaeI MboI
MboII MnlI MspI NdeII NlaIII
Sau3AI ScrFI SfaNI TaqI TfiI
TseI TthHB8I XbaI