################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## Acc65I AccI AccIII AclI AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BciVI BclI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrGI BssHII Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraII DrdI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HgaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 647 GACGTC GACGT^C AcyI 647 GACGTC G[GA]^CG[CT]C AflII 785 CTTAAG C^TTAAG AhdI 477 GACCATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 679 GATCC GGATC(4/5) AspEI 477 GACCATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 366 GGACC G^G[AT]CC BanI 136 GGTGCC G^G[CT][GA]CC BbvCI 403 CCTCAGC CCTCAGC(-5/-2) BcgI 158 GCATCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 70 CCCGG CC^[GC]GG BfrI 785 CTTAAG C^TTAAG BglI 51 GCCAGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 647 GACGTC G[GA]^CG[CT]C BsaI 977 GAGACC GGTCTC(1/5) BsiWI 75 CGTACG C^GTACG BsmAI 977 GAGAC GTCTC(1/5) BsmFI 637 GTCCC GGGAC(10/14) BspHI 628 TCATGA T^CATGA BsrBI 12 GAGCGG CCGCTC(-3/-3) BsrDI 384 CATTGC GCAATG(2/0) BsrFI 17 GCCGGC [GA]^CCGG[CT] BssSI 838 CTCGTG CACGAG(-5/-1) BstNI 52 CCAGG CC^[AT]GG BstYI 678 AGATCC [GA]^GATC[CT] CfoI 113 GCGC GCG^C Cfr10I 17 GCCGGC [GA]^CCGG[CT] DraI 273 TTTAAA TTT^AAA DraIII 341 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 15 CGGCCG [CT]^GGCC[GA] EagI 15 CGGCCG C^GGCCG Eam1105I 477 GACCATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 15 CGGCCG C^GGCCG Eco52I 15 CGGCCG C^GGCCG EcoRII 52 CCAGG ^CC[AT]GG HaeII 112 AGCGCC [GA]GCGC^[CT] HhaI 113 GCGC GCG^C Hin1I 647 GACGTC G[GA]^CG[CT]C HinP1I 113 GCGC G^CGC HincII 754 GTTAAC GT[CT]^[GA]AC HindII 754 GTTAAC GT[CT]^[GA]AC HpaI 754 GTTAAC GTT^AAC MaeII 648 ACGT A^CGT MflI 678 AGATCC [GA]^GATC[CT] MslI 384 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 52 CCAGG CC^[AT]GG NaeI 17 GCCGGC GCC^GGC NciI 70 CCCGG CC^[GC]GG NgoMIV 17 GCCGGC G^CCGGC NlaIV 136 GGTGCC GG[ACGT]^[ACGT]CC NspI 791 ACATGC [GA]CATG^[CT] PleI 432 GACTC GAGTC(4/5) PvuI 29 CGATCG CGAT^CG PvuII 409 CAGCTG CAG^CTG RcaI 628 TCATGA T^CATGA SmlI 785 CTTAAG C^T[CT][GA]AG TfiI 864 GAATC G^A[AT]TC XhoII 678 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 65 CGCG CG^CG 123 CGCG AcsI 298 AAATTC [GA]^AATT[CT] 938 AAATTT AfaI 76 GTAC GT^AC 153 GTAC AlwNI 406 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 409 CAGCTGCTG ApoI 298 AAATTC [GA]^AATT[CT] 938 AAATTT Asp700I 286 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 297 GAAATTCTTC AspHI 23 GTGCTC G[AT]GC[AT]^C 500 GTGCTC BfaI 337 CTAG C^TAG 902 CTAG BsaJI 64 CCGCGG C^C[ACGT][ACGT]GG 122 CCGCGG BsiHKAI 23 GTGCTC G[AT]GC[AT]^C 500 GTGCTC BsiYI 46 CCGCTGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 64 CCGCGGCCCGG BslI 46 CCGCTGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 64 CCGCGGCCCGG BssKI 52 CCAGG ^CC[ACGT]GG 70 CCCGG BstAPI 408 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 615 GCAAATTATGC BstUI 65 CGCG CG^CG 123 CGCG DpnII 30 GATC ^GATC 679 GATC DsaI 64 CCGCGG C^C[GA][CT]GG 122 CCGCGG FauI 56 GCGGG CCCGC(4/6) 124 GCGGG HindIII 232 AAGCTT A^AGCTT 469 AAGCTT HinfI 432 GACTC G^A[ACGT]TC 864 GAATC KspI 64 CCGCGG CCGC^GG 122 CCGCGG MaeI 337 CTAG C^TAG 902 CTAG MboI 30 GATC ^GATC 679 GATC MvnI 65 CGCG CG^CG 123 CGCG NdeII 30 GATC ^GATC 679 GATC RsaI 76 GTAC GT^AC 153 GTAC SacII 64 CCGCGG CCGC^GG 122 CCGCGG Sau3AI 30 GATC ^GATC 679 GATC ScrFI 52 CCAGG CC^[ACGT]GG 70 CCCGG Tsp45I 185 GTGAC ^GT[GC]AC 347 GTGAC XmnI 286 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 297 GAAATTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AluI BbvI BmyI BsiEI Bsp1286I BsrI Cac8I Cfr13I DdeI EarI Fnu4HI HaeIII HapII HpaII HphI ItaI Ksp632I MaeIII MboII MnlI MseI MspA1I MspI MwoI NlaIII Sau96I SfaNI TaqI Tru9I TseI Tsp509I TspRI TthHB8I