##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
Acc65I AccI AccIII AclI AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BciVI BclI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BseRI BsgI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrGI BssHII Bst1107I
BstBI BstEII BstF5I BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DrdI Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HgaI KasI KpnI MamI MfeI
MluI MluNI MroI MscI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 647 GACGTC GACGT^C
AcyI 647 GACGTC G[GA]^CG[CT]C
AflII 785 CTTAAG C^TTAAG
AhdI 477 GACCATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 679 GATCC GGATC(4/5)
AspEI 477 GACCATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 366 GGACC G^G[AT]CC
BanI 136 GGTGCC G^G[CT][GA]CC
BbvCI 403 CCTCAGC CCTCAGC(-5/-2)
BcgI 158 GCATCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 70 CCCGG CC^[GC]GG
BfrI 785 CTTAAG C^TTAAG
BglI 51 GCCAGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 647 GACGTC G[GA]^CG[CT]C
BsaI 977 GAGACC GGTCTC(1/5)
BsiWI 75 CGTACG C^GTACG
BsmAI 977 GAGAC GTCTC(1/5)
BsmFI 637 GTCCC GGGAC(10/14)
BspHI 628 TCATGA T^CATGA
BsrBI 12 GAGCGG CCGCTC(-3/-3)
BsrDI 384 CATTGC GCAATG(2/0)
BsrFI 17 GCCGGC [GA]^CCGG[CT]
BssSI 838 CTCGTG CACGAG(-5/-1)
BstNI 52 CCAGG CC^[AT]GG
BstYI 678 AGATCC [GA]^GATC[CT]
CfoI 113 GCGC GCG^C
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
DraI 273 TTTAAA TTT^AAA
DraIII 341 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 15 CGGCCG [CT]^GGCC[GA]
EagI 15 CGGCCG C^GGCCG
Eam1105I 477 GACCATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 15 CGGCCG C^GGCCG
Eco52I 15 CGGCCG C^GGCCG
EcoRII 52 CCAGG ^CC[AT]GG
HaeII 112 AGCGCC [GA]GCGC^[CT]
HhaI 113 GCGC GCG^C
Hin1I 647 GACGTC G[GA]^CG[CT]C
HinP1I 113 GCGC G^CGC
HincII 754 GTTAAC GT[CT]^[GA]AC
HindII 754 GTTAAC GT[CT]^[GA]AC
HpaI 754 GTTAAC GTT^AAC
MaeII 648 ACGT A^CGT
MflI 678 AGATCC [GA]^GATC[CT]
MslI 384 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 52 CCAGG CC^[AT]GG
NaeI 17 GCCGGC GCC^GGC
NciI 70 CCCGG CC^[GC]GG
NgoMIV 17 GCCGGC G^CCGGC
NlaIV 136 GGTGCC GG[ACGT]^[ACGT]CC
NspI 791 ACATGC [GA]CATG^[CT]
PleI 432 GACTC GAGTC(4/5)
PvuI 29 CGATCG CGAT^CG
PvuII 409 CAGCTG CAG^CTG
RcaI 628 TCATGA T^CATGA
SmlI 785 CTTAAG C^T[CT][GA]AG
TfiI 864 GAATC G^A[AT]TC
XhoII 678 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 65 CGCG CG^CG
123 CGCG
AcsI 298 AAATTC [GA]^AATT[CT]
938 AAATTT
AfaI 76 GTAC GT^AC
153 GTAC
AlwNI 406 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
409 CAGCTGCTG
ApoI 298 AAATTC [GA]^AATT[CT]
938 AAATTT
Asp700I 286 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
297 GAAATTCTTC
AspHI 23 GTGCTC G[AT]GC[AT]^C
500 GTGCTC
BfaI 337 CTAG C^TAG
902 CTAG
BsaJI 64 CCGCGG C^C[ACGT][ACGT]GG
122 CCGCGG
BsiHKAI 23 GTGCTC G[AT]GC[AT]^C
500 GTGCTC
BsiYI 46 CCGCTGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
64 CCGCGGCCCGG
BslI 46 CCGCTGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
64 CCGCGGCCCGG
BssKI 52 CCAGG ^CC[ACGT]GG
70 CCCGG
BstAPI 408 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
615 GCAAATTATGC
BstUI 65 CGCG CG^CG
123 CGCG
DpnII 30 GATC ^GATC
679 GATC
DsaI 64 CCGCGG C^C[GA][CT]GG
122 CCGCGG
FauI 56 GCGGG CCCGC(4/6)
124 GCGGG
HindIII 232 AAGCTT A^AGCTT
469 AAGCTT
HinfI 432 GACTC G^A[ACGT]TC
864 GAATC
KspI 64 CCGCGG CCGC^GG
122 CCGCGG
MaeI 337 CTAG C^TAG
902 CTAG
MboI 30 GATC ^GATC
679 GATC
MvnI 65 CGCG CG^CG
123 CGCG
NdeII 30 GATC ^GATC
679 GATC
RsaI 76 GTAC GT^AC
153 GTAC
SacII 64 CCGCGG CCGC^GG
122 CCGCGG
Sau3AI 30 GATC ^GATC
679 GATC
ScrFI 52 CCAGG CC^[ACGT]GG
70 CCCGG
Tsp45I 185 GTGAC ^GT[GC]AC
347 GTGAC
XmnI 286 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
297 GAAATTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BbvI BmyI BsiEI
Bsp1286I BsrI Cac8I Cfr13I DdeI
EarI Fnu4HI HaeIII HapII HpaII
HphI ItaI Ksp632I MaeIII MboII
MnlI MseI MspA1I MspI MwoI
NlaIII Sau96I SfaNI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I