##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BlnI
BsaAI BsaBI BsaI BseAI BsgI
BsiWI BsmI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraIII
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MamI MluI MroI MslI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1034 GACGTC GACGT^C
AspHI 1059 GTGCTC G[AT]GC[AT]^C
BalI 528 TGGCCA TGG^CCA
BbsI 599 GAAGAC GAAGAC(2/6)
BbvCI 41 GCTGAGG CCTCAGC(-5/-2)
BglI 108 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 599 GAAGAC GAAGAC(2/6)
BsiHKAI 1059 GTGCTC G[AT]GC[AT]^C
Bsp1407I 407 TGTACA T^GTACA
BspHI 1063 TCATGA T^CATGA
BsrFI 65 GCCGGC [GA]^CCGG[CT]
BsrGI 407 TGTACA T^GTACA
BssHII 250 GCGCGC G^CGCGC
BstXI 531 CCAATACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 65 GCCGGC [GA]^CCGG[CT]
DraI 1102 TTTAAA TTT^AAA
DrdI 320 GACGGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 610 GAAGAG CTCTTC(1/4)
EcoT14I 735 CCATGG C^C[AT][AT]GG
Ksp632I 610 GAAGAG CTCTTC(1/4)
KspI 357 CCGCGG CCGC^GG
MaeII 1035 ACGT A^CGT
MfeI 389 CAATTG C^AATTG
MluNI 528 TGGCCA TGG^CCA
MscI 528 TGGCCA TGG^CCA
MunI 389 CAATTG C^AATTG
NaeI 65 GCCGGC GCC^GGC
NcoI 735 CCATGG C^CATGG
NgoMIV 65 GCCGGC G^CCGGC
PleI 680 GAGTC GAGTC(4/5)
PpuMI 837 AGGACCT [GA]G^G[AT]CC[CT]
PstI 576 CTGCAG CTGCA^G
RcaI 1063 TCATGA T^CATGA
SacII 357 CCGCGG CCGC^GG
SfcI 576 CTGCAG C^T[GA][CT]AG
SgrAI 64 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 407 TGTACA T^GTACA
SspI 1168 AATATT AAT^ATT
StyI 735 CCATGG C^C[AT][AT]GG
TaqI 519 TCGA T^CGA
TspRI 233 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 519 TCGA T^CGA
XbaI 505 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 785 CTTAAG C^TTAAG
908 CTTAAG
AlwI 175 GATCC GGATC(4/5)
304 GATCC
ApaI 717 GGGCCC GGGCC^C
858 GGGCCC
BbeI 323 GGCGCC GGCGC^C
337 GGCGCC
BfaI 506 CTAG C^TAG
822 CTAG
BfrI 785 CTTAAG C^TTAAG
908 CTTAAG
BglII 654 AGATCT A^GATCT
829 AGATCT
BlpI 160 GCTGAGC GC^T[ACGT]AGC
493 GCTGAGC
Bpu1102I 160 GCTGAGC GC^T[ACGT]AGC
493 GCTGAGC
BsaWI 383 TCCGGT [AT]^CCGG[AT]
630 TCCGGT
BsmAI 25 GAGAC GTCTC(1/5)
47 GAGAC
BsmBI 25 GAGACG CGTCTC(1/5)
47 GAGACG
BsmFI 318 GGGAC GGGAC(10/14)
1037 GTCCC
CelII 160 GCTGAGC GC^T[ACGT]AGC
493 GCTGAGC
DraII 717 GGGCCCT [GA]G^G[ACGT]CC[CT]
837 AGGACCT
EagI 138 CGGCCG C^GGCCG
294 CGGCCG
EclXI 138 CGGCCG C^GGCCG
294 CGGCCG
Eco52I 138 CGGCCG C^GGCCG
294 CGGCCG
EcoO109I 717 GGGCCCT [GA]G^G[ACGT]CC[CT]
837 AGGACCT
KasI 323 GGCGCC G^GCGCC
337 GGCGCC
MaeI 506 CTAG C^TAG
822 CTAG
MaeIII 329 GTCAC ^GT[ACGT]AC
1032 GTGAC
MspA1I 357 CCGCGG C[AC]G^C[GT]G
923 CAGCGG
NarI 323 GGCGCC GG^CGCC
337 GGCGCC
SfoI 323 GGCGCC GGC^GCC
337 GGCGCC
Tsp45I 329 GTCAC ^GT[GC]AC
1032 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiEI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MboI MboII MflI
MnlI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI TfiI Tru9I TseI Tsp509I
XhoII