##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AgeI
Alw44I AlwNI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BanI BbeI BbvCI BclI
BcnI BfaI BfrI BglII BlnI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I CfoI
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MslI MspA1I MunI MvnI NarI
NciI NcoI NdeI NheI NotI
NruI PacI PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tth111I
Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AflIII 787 ACGTGT A^C[GA][CT]GT
AhdI 345 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 347 CCTGAGG CC^T[ACGT]AGG
ApaI 828 GGGCCC GGGCC^C
Asp700I 45 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 345 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 375 GAGCAC G[AT]GC[AT]^C
AvaI 370 CTCGAG C^[CT]CG[GA]G
AvaII 352 GGTCC G^G[AT]CC
BalI 655 TGGCCA TGG^CCA
BamHI 728 GGATCC G^GATCC
BanII 828 GGGCCC G[GA]GC[CT]^C
BbrPI 786 CACGTG CAC^GTG
BbsI 45 GAAGAC GAAGAC(2/6)
BcgI 372 CGAGAGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 478 GGATAC GTATCC(6/5)
BglI 830 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2 GCTAAGC GC^T[ACGT]AGC
BpuAI 45 GAAGAC GAAGAC(2/6)
BsaAI 786 CACGTG [CT]AC^GT[GA]
BsaWI 316 ACCGGA [AT]^CCGG[AT]
BsiHKAI 375 GAGCAC G[AT]GC[AT]^C
BsmAI 99 GAGAC GTCTC(1/5)
BsoBI 370 CTCGAG C^[CT]CG[GA]G
BsrDI 446 CATTGC GCAATG(2/0)
BsrFI 610 GCCGGC [GA]^CCGG[CT]
BstBI 264 TTCGAA TT^CGAA
BstXI 658 CCAGCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 728 GGATCC [GA]^GATC[CT]
Bsu36I 347 CCTGAGG CC^T[ACGT]AGG
CelII 2 GCTAAGC GC^T[ACGT]AGC
Cfr10I 610 GCCGGC [GA]^CCGG[CT]
DraIII 590 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 65 CCGTGG C^C[GA][CT]GG
Eam1105I 345 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 347 CCTGAGG CC^T[ACGT]AGG
EcoRI 261 GAATTC G^AATTC
EcoT22I 698 ATGCAT ATGCA^T
HincII 234 GTTGAC GT[CT]^[GA]AC
HindII 234 GTTGAC GT[CT]^[GA]AC
MflI 728 GGATCC [GA]^GATC[CT]
MluNI 655 TGGCCA TGG^CCA
MscI 655 TGGCCA TGG^CCA
NaeI 610 GCCGGC GCC^GGC
NgoMIV 610 GCCGGC G^CCGGC
NsiI 698 ATGCAT ATGCA^T
NspV 264 TTCGAA TT^CGAA
PaeR7I 370 CTCGAG C^TCGAG
PmaCI 786 CACGTG CAC^GTG
PmlI 786 CACGTG CAC^GTG
PpuMI 351 AGGTCCC [GA]G^G[AT]CC[CT]
SfiI 829 GGCCCAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 264 TTCGAA TT^CGAA
SmlI 370 CTCGAG C^T[CT][GA]AG
StuI 643 AGGCCT AGG^CCT
XcmI 133 CCAGAGTCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 370 CTCGAG C^TCGAG
XhoII 728 GGATCC [GA]^GATC[CT]
XmnI 45 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 21 GTCTAC GT^[AC][GT]AC
342 GTAGAC
BmyI 375 GAGCAC G[AGT]GC[ACT]^C
828 GGGCCC
BseRI 274 GAGGAG GAGGAG(10/8)
639 GAGGAG
Bsp1286I 375 GAGCAC G[AGT]GC[ACT]^C
828 GGGCCC
BsrI 905 ACTGG ACTGG(1/-1)
121 CCAGT
DpnII 540 GATC ^GATC
729 GATC
DraII 351 AGGTCCC [GA]G^G[ACGT]CC[CT]
827 GGGGCCC
EaeI 62 TGGCCG [CT]^GGCC[GA]
655 TGGCCA
EarI 465 CTCTTC CTCTTC(1/4)
507 GAAGAG
EcoO109I 351 AGGTCCC [GA]G^G[ACGT]CC[CT]
827 GGGGCCC
EcoT14I 287 CCTTGG C^C[AT][AT]GG
483 CCAAGG
HapII 317 CCGG C^CGG
611 CCGG
HinfI 136 GAGTC G^A[ACGT]TC
844 GAGTC
HpaII 317 CCGG C^CGG
611 CCGG
Ksp632I 465 CTCTTC CTCTTC(1/4)
507 GAAGAG
MaeII 787 ACGT A^CGT
878 ACGT
MboI 540 GATC ^GATC
729 GATC
MspI 317 CCGG C^CGG
611 CCGG
NdeII 540 GATC ^GATC
729 GATC
NspI 614 GCATGT [GA]CATG^[CT]
696 ACATGC
PleI 136 GAGTC GAGTC(4/5)
844 GAGTC
Sau3AI 540 GATC ^GATC
729 GATC
StyI 287 CCTTGG C^C[AT][AT]GG
483 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BpmI BsaJI BsgI BsiYI BslI
BsmFI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EcoRII Fnu4HI FokI
HaeIII HphI ItaI MaeIII MboII
MnlI MseI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI SfaNI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I