##################################
# NO CUT ENZYMES (  172 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AflII       AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BcnI        BfrI        BglI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT22I     Fnu4HI      FokI        
FseI        FspI        HaeII       HaeIII      HincII      
HindII      HpaI        ItaI        KasI        KpnI        
KspI        MamI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        TseI        
Tth111I     XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AclI          679  AACGTT               AA^CGTT
AcyI          767  GACGCC               G[GA]^CG[CT]C
AfaI          730  GTAC                 GT^AC
AflIII        485  ACATGT               A^C[GA][CT]GT
AvaII         115  GGACC                G^G[AT]CC
AvrII         171  CCTAGG               C^CTAGG
BclI          413  TGATCA               T^GATCA
BfaI          172  CTAG                 C^TAG
BlnI          171  CCTAGG               C^CTAGG
BsaHI         767  GACGCC               G[GA]^CG[CT]C
BsaI            5  GAGACC               GGTCTC(1/5)
BsaWI         464  TCCGGT               [AT]^CCGG[AT]
BsmFI         317  GTCCC                GGGAC(10/14)
BspLU11I      485  ACATGT               A^CATGT
Cfr13I        115  GGACC                G^G[ACGT]CC
DpnII         414  GATC                 ^GATC
EarI           92  CTCTTC               CTCTTC(1/4)
EcoRI         303  GAATTC               G^AATTC
EcoT14I       171  CCTAGG               C^C[AT][AT]GG
FauI           24  GCGGG                CCCGC(4/6)
FbaI          413  TGATCA               T^GATCA
HapII         465  CCGG                 C^CGG
HgaI          767  GACGC                GACGC(5/10)
Hin1I         767  GACGCC               G[GA]^CG[CT]C
HinfI         327  GAGTC                G^A[ACGT]TC
HpaII         465  CCGG                 C^CGG
Ksp632I        92  CTCTTC               CTCTTC(1/4)
MaeI          172  CTAG                 C^TAG
MboI          414  GATC                 ^GATC
MfeI          514  CAATTG               C^AATTG
MslI          417  CACAGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          465  CCGG                 C^CGG
MunI          514  CAATTG               C^AATTG
MwoI          159  GCTTCTCAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         414  GATC                 ^GATC
PflMI         739  CCATTTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          327  GAGTC                GAGTC(4/5)
Psp1406I      679  AACGTT               AA^CGTT
PstI           64  CTGCAG               CTGCA^G
RsaI          730  GTAC                 GT^AC
Sau3AI        414  GATC                 ^GATC
Sau96I        115  GGACC                G^G[ACGT]CC
SmlI          163  CTCAAG               C^T[CT][GA]AG
SphI          616  GCATGC               GCATG^C
StyI          171  CCTAGG               C^C[AT][AT]GG
Van91I        739  CCATTTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BseRI         188  CTCCTC               GAGGAG(10/8)
              640  CTCCTC              
BsiYI         170  CCCTAGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              739  CCATTTTATGG         
BslI          170  CCCTAGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              739  CCATTTTATGG         
BsrI          258  CCAGT                ACTGG(1/-1)
              367  CCAGT               
Cac8I         616  GCATGC               GC[ACGT]^[ACGT]GC
              827  GCACGC              
CfoI          269  GCGC                 GCG^C
              825  GCGC                
DraI           13  TTTAAA               TTT^AAA
              794  TTTAAA              
HhaI          269  GCGC                 GCG^C
              825  GCGC                
HinP1I        269  GCGC                 G^CGC
              825  GCGC                
HindIII       394  AAGCTT               A^AGCTT
              661  AAGCTT              
MaeII         130  ACGT                 A^CGT
              680  ACGT                
MaeIII         75  GTGAC                ^GT[ACGT]AC
              201  GTCAC               
SfaNI         735  GATGC                GCATC(5/9)
              295  GCATC               
SfcI           64  CTGCAG               C^T[GA][CT]AG
              143  CTACAG              
TaqI          222  TCGA                 T^CGA
              890  TCGA                
Tsp45I         75  GTGAC                ^GT[GC]AC
              201  GTCAC               
TspRI         368  CAGTG                CA[GC]TG[ACGT][ACGT]^
              751  CAGTG               
TthHB8I       222  TCGA                 T^CGA
              890  TCGA                
XcmI           10  CCATTTAAACGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              273  CCAACTCCCAAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BsaJI       
BsmAI       BssKI       BstNI       DdeI        EcoRII      
HphI        MboII       MnlI        MseI        MvaI        
NlaIII      NlaIV       NspI        ScrFI       Tru9I       
Tsp509I