##################################
# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BcnI BfrI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I Fnu4HI FokI
FseI FspI HaeII HaeIII HincII
HindII HpaI ItaI KasI KpnI
KspI MamI MflI MluI MluNI
MroI MscI MspA1I MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI TseI
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 679 AACGTT AA^CGTT
AcyI 767 GACGCC G[GA]^CG[CT]C
AfaI 730 GTAC GT^AC
AflIII 485 ACATGT A^C[GA][CT]GT
AvaII 115 GGACC G^G[AT]CC
AvrII 171 CCTAGG C^CTAGG
BclI 413 TGATCA T^GATCA
BfaI 172 CTAG C^TAG
BlnI 171 CCTAGG C^CTAGG
BsaHI 767 GACGCC G[GA]^CG[CT]C
BsaI 5 GAGACC GGTCTC(1/5)
BsaWI 464 TCCGGT [AT]^CCGG[AT]
BsmFI 317 GTCCC GGGAC(10/14)
BspLU11I 485 ACATGT A^CATGT
Cfr13I 115 GGACC G^G[ACGT]CC
DpnII 414 GATC ^GATC
EarI 92 CTCTTC CTCTTC(1/4)
EcoRI 303 GAATTC G^AATTC
EcoT14I 171 CCTAGG C^C[AT][AT]GG
FauI 24 GCGGG CCCGC(4/6)
FbaI 413 TGATCA T^GATCA
HapII 465 CCGG C^CGG
HgaI 767 GACGC GACGC(5/10)
Hin1I 767 GACGCC G[GA]^CG[CT]C
HinfI 327 GAGTC G^A[ACGT]TC
HpaII 465 CCGG C^CGG
Ksp632I 92 CTCTTC CTCTTC(1/4)
MaeI 172 CTAG C^TAG
MboI 414 GATC ^GATC
MfeI 514 CAATTG C^AATTG
MslI 417 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 465 CCGG C^CGG
MunI 514 CAATTG C^AATTG
MwoI 159 GCTTCTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 414 GATC ^GATC
PflMI 739 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 327 GAGTC GAGTC(4/5)
Psp1406I 679 AACGTT AA^CGTT
PstI 64 CTGCAG CTGCA^G
RsaI 730 GTAC GT^AC
Sau3AI 414 GATC ^GATC
Sau96I 115 GGACC G^G[ACGT]CC
SmlI 163 CTCAAG C^T[CT][GA]AG
SphI 616 GCATGC GCATG^C
StyI 171 CCTAGG C^C[AT][AT]GG
Van91I 739 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BseRI 188 CTCCTC GAGGAG(10/8)
640 CTCCTC
BsiYI 170 CCCTAGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
739 CCATTTTATGG
BslI 170 CCCTAGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
739 CCATTTTATGG
BsrI 258 CCAGT ACTGG(1/-1)
367 CCAGT
Cac8I 616 GCATGC GC[ACGT]^[ACGT]GC
827 GCACGC
CfoI 269 GCGC GCG^C
825 GCGC
DraI 13 TTTAAA TTT^AAA
794 TTTAAA
HhaI 269 GCGC GCG^C
825 GCGC
HinP1I 269 GCGC G^CGC
825 GCGC
HindIII 394 AAGCTT A^AGCTT
661 AAGCTT
MaeII 130 ACGT A^CGT
680 ACGT
MaeIII 75 GTGAC ^GT[ACGT]AC
201 GTCAC
SfaNI 735 GATGC GCATC(5/9)
295 GCATC
SfcI 64 CTGCAG C^T[GA][CT]AG
143 CTACAG
TaqI 222 TCGA T^CGA
890 TCGA
Tsp45I 75 GTGAC ^GT[GC]AC
201 GTCAC
TspRI 368 CAGTG CA[GC]TG[ACGT][ACGT]^
751 CAGTG
TthHB8I 222 TCGA T^CGA
890 TCGA
XcmI 10 CCATTTAAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
273 CCAACTCCCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AcsI AluI ApoI BsaJI
BsmAI BssKI BstNI DdeI EcoRII
HphI MboII MnlI MseI MvaI
NlaIII NlaIV NspI ScrFI Tru9I
Tsp509I