################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BcnI BfrI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I Fnu4HI FokI FseI FspI HaeII HaeIII HincII HindII HpaI ItaI KasI KpnI KspI MamI MflI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI TseI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AclI 679 AACGTT AA^CGTT AcyI 767 GACGCC G[GA]^CG[CT]C AfaI 730 GTAC GT^AC AflIII 485 ACATGT A^C[GA][CT]GT AvaII 115 GGACC G^G[AT]CC AvrII 171 CCTAGG C^CTAGG BclI 413 TGATCA T^GATCA BfaI 172 CTAG C^TAG BlnI 171 CCTAGG C^CTAGG BsaHI 767 GACGCC G[GA]^CG[CT]C BsaI 5 GAGACC GGTCTC(1/5) BsaWI 464 TCCGGT [AT]^CCGG[AT] BsmFI 317 GTCCC GGGAC(10/14) BspLU11I 485 ACATGT A^CATGT Cfr13I 115 GGACC G^G[ACGT]CC DpnII 414 GATC ^GATC EarI 92 CTCTTC CTCTTC(1/4) EcoRI 303 GAATTC G^AATTC EcoT14I 171 CCTAGG C^C[AT][AT]GG FauI 24 GCGGG CCCGC(4/6) FbaI 413 TGATCA T^GATCA HapII 465 CCGG C^CGG HgaI 767 GACGC GACGC(5/10) Hin1I 767 GACGCC G[GA]^CG[CT]C HinfI 327 GAGTC G^A[ACGT]TC HpaII 465 CCGG C^CGG Ksp632I 92 CTCTTC CTCTTC(1/4) MaeI 172 CTAG C^TAG MboI 414 GATC ^GATC MfeI 514 CAATTG C^AATTG MslI 417 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 465 CCGG C^CGG MunI 514 CAATTG C^AATTG MwoI 159 GCTTCTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 414 GATC ^GATC PflMI 739 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 327 GAGTC GAGTC(4/5) Psp1406I 679 AACGTT AA^CGTT PstI 64 CTGCAG CTGCA^G RsaI 730 GTAC GT^AC Sau3AI 414 GATC ^GATC Sau96I 115 GGACC G^G[ACGT]CC SmlI 163 CTCAAG C^T[CT][GA]AG SphI 616 GCATGC GCATG^C StyI 171 CCTAGG C^C[AT][AT]GG Van91I 739 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BseRI 188 CTCCTC GAGGAG(10/8) 640 CTCCTC BsiYI 170 CCCTAGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 739 CCATTTTATGG BslI 170 CCCTAGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 739 CCATTTTATGG BsrI 258 CCAGT ACTGG(1/-1) 367 CCAGT Cac8I 616 GCATGC GC[ACGT]^[ACGT]GC 827 GCACGC CfoI 269 GCGC GCG^C 825 GCGC DraI 13 TTTAAA TTT^AAA 794 TTTAAA HhaI 269 GCGC GCG^C 825 GCGC HinP1I 269 GCGC G^CGC 825 GCGC HindIII 394 AAGCTT A^AGCTT 661 AAGCTT MaeII 130 ACGT A^CGT 680 ACGT MaeIII 75 GTGAC ^GT[ACGT]AC 201 GTCAC SfaNI 735 GATGC GCATC(5/9) 295 GCATC SfcI 64 CTGCAG C^T[GA][CT]AG 143 CTACAG TaqI 222 TCGA T^CGA 890 TCGA Tsp45I 75 GTGAC ^GT[GC]AC 201 GTCAC TspRI 368 CAGTG CA[GC]TG[ACGT][ACGT]^ 751 CAGTG TthHB8I 222 TCGA T^CGA 890 TCGA XcmI 10 CCATTTAAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 273 CCAACTCCCAAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI AcsI AluI ApoI BsaJI BsmAI BssKI BstNI DdeI EcoRII HphI MboII MnlI MseI MvaI NlaIII NlaIV NspI ScrFI Tru9I Tsp509I